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The map label for this gene is prsA [H]

Identifier: 52144275

GI number: 52144275

Start: 1063238

End: 1064101

Strand: Reverse

Name: prsA [H]

Synonym: BCZK0952

Alternate gene names: 52144275

Gene position: 1064101-1063238 (Counterclockwise)

Preceding gene: 52144272

Following gene: 52144276

Centisome position: 20.07

GC content: 33.68

Gene sequence:

>864_bases
ATGAAGAAAGCTATGCTTGCCTTAGCCGCAACAAGTGTAATCGCATTATCAGCATGTGGAACATCATCATCAGACAAAAT
CGTTACATCTAAAGCTGGTGACATTACAAAAGACGAATTCTACGAGCAAATGAAGACACAAGCTGGTAAACAAGTGTTAA
ACAACATGGTTATGGAAAAAGTACTTATTAAAAACTACAAAGTTGAAGACAAAGAAGTAGACAAAAAGTACGATGAAATG
AAAAAACAATACGGTGATCAATTTGATACACTATTAAAACAACAAGGTATTAAAGAAGGAACGTTAAAAACTGGTGTGCG
TGCTCAATTAGCGCAAGAAAAAGCTATCGAGAAAACAATCACTGATAAAGAATTAAAAGAAAACTACAAGCCTGAAATTA
AAGCAAGCCACATTCTTGTAAAAGATGAAGCGACTGCGAAAAAGGTTAAAGAAGAACTTGGACAAGGTAAATCTTTCGAA
GAGTTAGCGAAACAATACTCTGAAGATACTGGTTCAAAAGAAAAAGGTGGAGACTTAGGATTCTTCGGTCCTGGTAAAAT
GGTTAAAGAATTCGAAGATGCTGCTTATAAACTGAAAAAAGATGAAGTAAGCGAGCCTGTGAAATCACAATTCGGTTACC
ACATCATTAAAGTAACAGAAATTAAAGAACCAGAAAAATCATTTGAACAATCAAAAGTTGACATCAAAAAAGAAATCGTT
CAGAAGAAAGCGCAAGATGCTGAATTCATGAACGAACTTATGATGAAAGAAATCAAAAAAGCTGACGTAAAAGTTGACGA
TAAAGATTTAAAAGATCTTTTCGAAGAGAAAAAAGCTGACGCTAAAAAAGAAGAAAAGAAATAA

Upstream 100 bases:

>100_bases
AAAAGCAAAAGTATCAGTGAGACTTAATCGGGCTCTAATGAACTAAAGTTCATTGGTATAGGATAAATTAAAAATACATA
ATAGTAGGAGTGTTTATCGA

Downstream 100 bases:

>100_bases
TGAAATAAAAAGCTCGGGCTTAGGCCCGAGCTTTTTTTGTATACCCTCTTCTCTCGGTAGTTATGTCCGATTCGGCAAAT
GAAATTATATAGTTGGACCT

Product: peptidylprolyl isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKKAMLALAATSVIALSACGTSSSDKIVTSKAGDITKDEFYEQMKTQAGKQVLNNMVMEKVLIKNYKVEDKEVDKKYDEM
KKQYGDQFDTLLKQQGIKEGTLKTGVRAQLAQEKAIEKTITDKELKENYKPEIKASHILVKDEATAKKVKEELGQGKSFE
ELAKQYSEDTGSKEKGGDLGFFGPGKMVKEFEDAAYKLKKDEVSEPVKSQFGYHIIKVTEIKEPEKSFEQSKVDIKKEIV
QKKAQDAEFMNELMMKEIKKADVKVDDKDLKDLFEEKKADAKKEEKK

Sequences:

>Translated_287_residues
MKKAMLALAATSVIALSACGTSSSDKIVTSKAGDITKDEFYEQMKTQAGKQVLNNMVMEKVLIKNYKVEDKEVDKKYDEM
KKQYGDQFDTLLKQQGIKEGTLKTGVRAQLAQEKAIEKTITDKELKENYKPEIKASHILVKDEATAKKVKEELGQGKSFE
ELAKQYSEDTGSKEKGGDLGFFGPGKMVKEFEDAAYKLKKDEVSEPVKSQFGYHIIKVTEIKEPEKSFEQSKVDIKKEIV
QKKAQDAEFMNELMMKEIKKADVKVDDKDLKDLFEEKKADAKKEEKK
>Mature_287_residues
MKKAMLALAATSVIALSACGTSSSDKIVTSKAGDITKDEFYEQMKTQAGKQVLNNMVMEKVLIKNYKVEDKEVDKKYDEM
KKQYGDQFDTLLKQQGIKEGTLKTGVRAQLAQEKAIEKTITDKELKENYKPEIKASHILVKDEATAKKVKEELGQGKSFE
ELAKQYSEDTGSKEKGGDLGFFGPGKMVKEFEDAAYKLKKDEVSEPVKSQFGYHIIKVTEIKEPEKSFEQSKVDIKKEIV
QKKAQDAEFMNELMMKEIKKADVKVDDKDLKDLFEEKKADAKKEEKK

Specific function: Plays a major role in protein secretion by helping the post-translocational extracellular folding of several secreted proteins. Important for the secretion of the protective antigen. The three psrA proteins in this organism show different but overlapping

COG id: COG0760

COG function: function code O; Parvulin-like peptidyl-prolyl isomerase

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PpiC domain [H]

Homologues:

Organism=Homo sapiens, GI38679892, Length=92, Percent_Identity=41.304347826087, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1786238, Length=106, Percent_Identity=34.9056603773585, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790211, Length=84, Percent_Identity=44.047619047619, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17537235, Length=92, Percent_Identity=41.304347826087, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322478, Length=60, Percent_Identity=56.6666666666667, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI21356303, Length=92, Percent_Identity=41.304347826087, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023059
- InterPro:   IPR000297
- InterPro:   IPR023058
- InterPro:   IPR008880 [H]

Pfam domain/function: PF00639 Rotamase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 32536; Mature: 32536

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01096 PPIC_PPIASE_1 ; PS50198 PPIC_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAMLALAATSVIALSACGTSSSDKIVTSKAGDITKDEFYEQMKTQAGKQVLNNMVMEK
CCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VLIKNYKVEDKEVDKKYDEMKKQYGDQFDTLLKQQGIKEGTLKTGVRAQLAQEKAIEKTI
HHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
TDKELKENYKPEIKASHILVKDEATAKKVKEELGQGKSFEELAKQYSEDTGSKEKGGDLG
HHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCC
FFGPGKMVKEFEDAAYKLKKDEVSEPVKSQFGYHIIKVTEIKEPEKSFEQSKVDIKKEIV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
QKKAQDAEFMNELMMKEIKKADVKVDDKDLKDLFEEKKADAKKEEKK
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKAMLALAATSVIALSACGTSSSDKIVTSKAGDITKDEFYEQMKTQAGKQVLNNMVMEK
CCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VLIKNYKVEDKEVDKKYDEMKKQYGDQFDTLLKQQGIKEGTLKTGVRAQLAQEKAIEKTI
HHHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
TDKELKENYKPEIKASHILVKDEATAKKVKEELGQGKSFEELAKQYSEDTGSKEKGGDLG
HHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCC
FFGPGKMVKEFEDAAYKLKKDEVSEPVKSQFGYHIIKVTEIKEPEKSFEQSKVDIKKEIV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
QKKAQDAEFMNELMMKEIKKADVKVDDKDLKDLFEEKKADAKKEEKK
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12721629; 12606539 [H]