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The map label for this gene is blaI [H]

Identifier: 52144236

GI number: 52144236

Start: 1100907

End: 1101305

Strand: Direct

Name: blaI [H]

Synonym: BCZK0993

Alternate gene names: 52144236

Gene position: 1100907-1101305 (Clockwise)

Preceding gene: 52144234

Following gene: 52144233

Centisome position: 20.77

GC content: 33.83

Gene sequence:

>399_bases
ATGACAAATCAATTACCTAAAATATCTGAAGCAGAATTAGAAATTATGAAAGTACTTTGGTCGAAATCTCCACAAACAGC
GAATGAAATTATAGAAGAACTGGAAGATGCAATGGACTGGAAACCGAAAACAATCCGCACATTAATTAATCGTTTAGTAC
AAAAGGAAGCTGTTTCTTACCATCAAGATAAAGGACGTATGTACGCTTATTATCCGTTAGTATCACAAGATAATTATTTG
CAAGTGGAAACAAAATCTTTACTAAAGCGTTTTTGCGGTGCAGCGTTTAAACCATTACTTGTCAATTTCTTAAAAGAGGA
AAAATTGTCTTCAGAAGATATTAACGAACTAAAACGCATTTTAGACGAGAAGACCGAGGAGAATAAGAGGAAGGATTGA

Upstream 100 bases:

>100_bases
TTGAAATTAGTTTTTTTCTAAAATATGGTTGACAATTAAGTATTACATTTGTAATCTTAGTTATAGATTACAAATGTAAT
ACGAGGAAGTGAAATATATT

Downstream 100 bases:

>100_bases
TAAAAATGATAGACATGCTTATAAAGGTATACCTTCCTCATTTTTTTGATTGGCTTATAGAAACGTCTCTTATGGCTAGT
ATATTAGTAGGATTTATTTT

Product: beta-lactamase (penicillinase) repressor

Products: substituted beta-amino acid

Alternate protein names: Beta-lactamase repressor protein; Regulatory protein BlaI [H]

Number of amino acids: Translated: 132; Mature: 131

Protein sequence:

>132_residues
MTNQLPKISEAELEIMKVLWSKSPQTANEIIEELEDAMDWKPKTIRTLINRLVQKEAVSYHQDKGRMYAYYPLVSQDNYL
QVETKSLLKRFCGAAFKPLLVNFLKEEKLSSEDINELKRILDEKTEENKRKD

Sequences:

>Translated_132_residues
MTNQLPKISEAELEIMKVLWSKSPQTANEIIEELEDAMDWKPKTIRTLINRLVQKEAVSYHQDKGRMYAYYPLVSQDNYL
QVETKSLLKRFCGAAFKPLLVNFLKEEKLSSEDINELKRILDEKTEENKRKD
>Mature_131_residues
TNQLPKISEAELEIMKVLWSKSPQTANEIIEELEDAMDWKPKTIRTLINRLVQKEAVSYHQDKGRMYAYYPLVSQDNYLQ
VETKSLLKRFCGAAFKPLLVNFLKEEKLSSEDINELKRILDEKTEENKRKD

Specific function: Transcriptional repressor that constitutively blocks expression of beta-lactamase. Regulates genes involved in antibiotic resistance. Binds DNA as a dimer [H]

COG id: COG3682

COG function: function code K; Predicted transcriptional regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BlaI transcriptional regulatory family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005650
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03965 Pencillinase_R [H]

EC number: 3.5.2.6

Molecular weight: Translated: 15551; Mature: 15420

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNQLPKISEAELEIMKVLWSKSPQTANEIIEELEDAMDWKPKTIRTLINRLVQKEAVSY
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
HQDKGRMYAYYPLVSQDNYLQVETKSLLKRFCGAAFKPLLVNFLKEEKLSSEDINELKRI
HHCCCCEEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LDEKTEENKRKD
HHHHHHHHCCCC
>Mature Secondary Structure 
TNQLPKISEAELEIMKVLWSKSPQTANEIIEELEDAMDWKPKTIRTLINRLVQKEAVSY
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
HQDKGRMYAYYPLVSQDNYLQVETKSLLKRFCGAAFKPLLVNFLKEEKLSSEDINELKRI
HHCCCCEEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LDEKTEENKRKD
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: beta-lactam; H2O

Specific reaction: A beta-lactam + H2O = a substituted beta-amino acid

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3096969; 3305074; 3260234; 3040663; 3498148 [H]