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The map label for this gene is hisF [H]

Identifier: 52143934

GI number: 52143934

Start: 1388270

End: 1389028

Strand: Direct

Name: hisF [H]

Synonym: BCZK1295

Alternate gene names: 52143934

Gene position: 1388270-1389028 (Clockwise)

Preceding gene: 52143935

Following gene: 52143932

Centisome position: 26.19

GC content: 40.84

Gene sequence:

>759_bases
ATGTTAGCGAAACGCATTATTCCATGTCTAGATGTGAAAGAAGGGCGAGTCGTAAAGGGTGTAAATTTTATAGGGTTACA
AGATGTCGGTGATCCTGTTGAAATAGCAGCTTTATATAATGATGCGGGAGCAGATGAAATAGTATTTTTAGATATTACGG
CAACACATGAAGGACGAAAAACAATTATAGATGTTGTAGAAAAAACGGCTTCAAAAGTATTTATTCCTCTTACAGTTGGC
GGAGGAATTTCAAGTGTGAAAGATATGTACAATTTACTAAGAGCGGGAGCAGATAAAGTTTCAATTAACTCAGCAGCAGT
GCGAAATCCAAAATTAATCGAAGAAGGGGCACAGCACTTTGGTTCACAATGTATTGTCGTCGCAATTGATGCTAGAAAAG
TAGCAGAAGGTAAGTGGAATGTATATGTGAACGGCGGAAGAGTTGATACGGGAATGGATGCAATCGAGTGGGCAAAGTGC
GTTGTTATGCTCGGGGCCGGCGAAATTTTGTTAACGAGTATGGATGCAGATGGAACGAAAAATGGATATGACCTTCGTTT
AACAGAAGAAATTTCAAAAAGTGTTTCCGTACCAGTCATCGCGTCAGGTGGATGTGGTCATGCTGATCACATTATAGAAG
TGTTTCAAAAGACAACAGTTGATGCAGCGTTAGCGGCATCAATCTTTCATTACGGTGAGGCGACGATAGGGGGCGTAAAG
AGAAAGTTAAGAAATGCAAACGTCGAGGTGCGGTTATGA

Upstream 100 bases:

>100_bases
TGAAAAAGTTAAATGATATGAACATATACGGCGTCATAATTGGTAAGGCTCTTTACGAGAAAACGATTGATTTAGAAGAA
GTACTAGAGGTAACAAAGTT

Downstream 100 bases:

>100_bases
AACCTAACTTTTCAAAAGGATTACTACCAGCAGTTGTAATTGAAGAAGGTACAAAAGAAGTTTTAATGCTAGCTTATATG
AATGAAGAAGCATATGAAAA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAALYNDAGADEIVFLDITATHEGRKTIIDVVEKTASKVFIPLTVG
GGISSVKDMYNLLRAGADKVSINSAAVRNPKLIEEGAQHFGSQCIVVAIDARKVAEGKWNVYVNGGRVDTGMDAIEWAKC
VVMLGAGEILLTSMDADGTKNGYDLRLTEEISKSVSVPVIASGGCGHADHIIEVFQKTTVDAALAASIFHYGEATIGGVK
RKLRNANVEVRL

Sequences:

>Translated_252_residues
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAALYNDAGADEIVFLDITATHEGRKTIIDVVEKTASKVFIPLTVG
GGISSVKDMYNLLRAGADKVSINSAAVRNPKLIEEGAQHFGSQCIVVAIDARKVAEGKWNVYVNGGRVDTGMDAIEWAKC
VVMLGAGEILLTSMDADGTKNGYDLRLTEEISKSVSVPVIASGGCGHADHIIEVFQKTTVDAALAASIFHYGEATIGGVK
RKLRNANVEVRL
>Mature_252_residues
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAALYNDAGADEIVFLDITATHEGRKTIIDVVEKTASKVFIPLTVG
GGISSVKDMYNLLRAGADKVSINSAAVRNPKLIEEGAQHFGSQCIVVAIDARKVAEGKWNVYVNGGRVDTGMDAIEWAKC
VVMLGAGEILLTSMDADGTKNGYDLRLTEEISKSVSVPVIASGGCGHADHIIEVFQKTTVDAALAASIFHYGEATIGGVK
RKLRNANVEVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=46.8992248062016, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI87082028, Length=210, Percent_Identity=29.5238095238095, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=33.7579617834395, Blast_Score=156, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 26821; Mature: 26821

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAALYNDAGADEIVFLDITATHEGRK
CCHHHCCCCCCCCCCCEEECEEEEEECCCCCCEEEEEEECCCCCCEEEEEEEEECCCCHH
TIIDVVEKTASKVFIPLTVGGGISSVKDMYNLLRAGADKVSINSAAVRNPKLIEEGAQHF
HHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCHHCCCCHHHHHHHHHH
GSQCIVVAIDARKVAEGKWNVYVNGGRVDTGMDAIEWAKCVVMLGAGEILLTSMDADGTK
CCEEEEEEEECEECCCCEEEEEEECCEECCCCHHHHHHHEEEEECCCEEEEEECCCCCCC
NGYDLRLTEEISKSVSVPVIASGGCGHADHIIEVFQKTTVDAALAASIFHYGEATIGGVK
CCEEEEEHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RKLRNANVEVRL
HHHHCCCEEEEC
>Mature Secondary Structure
MLAKRIIPCLDVKEGRVVKGVNFIGLQDVGDPVEIAALYNDAGADEIVFLDITATHEGRK
CCHHHCCCCCCCCCCCEEECEEEEEECCCCCCEEEEEEECCCCCCEEEEEEEEECCCCHH
TIIDVVEKTASKVFIPLTVGGGISSVKDMYNLLRAGADKVSINSAAVRNPKLIEEGAQHF
HHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHCCCCEEEECCHHCCCCHHHHHHHHHH
GSQCIVVAIDARKVAEGKWNVYVNGGRVDTGMDAIEWAKCVVMLGAGEILLTSMDADGTK
CCEEEEEEEECEECCCCEEEEEEECCEECCCCHHHHHHHEEEEECCCEEEEEECCCCCCC
NGYDLRLTEEISKSVSVPVIASGGCGHADHIIEVFQKTTVDAALAASIFHYGEATIGGVK
CCEEEEEHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RKLRNANVEVRL
HHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA