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The map label for this gene is amiB [H]

Identifier: 52143606

GI number: 52143606

Start: 1713237

End: 1714478

Strand: Direct

Name: amiB [H]

Synonym: BCZK1628

Alternate gene names: 52143606

Gene position: 1713237-1714478 (Clockwise)

Preceding gene: 52143608

Following gene: 52143605

Centisome position: 32.32

GC content: 35.99

Gene sequence:

>1242_bases
ATGAAAAATAGAATAATCGCAGCAGGAGTAATCGCAGCTAGTATATTATCTTATTCATCTAGTAGTTTCGCACAAACAAA
AACGTTTCCAGATGTCCAAGCAGGGCACTGGGCAGAAGATTCTATTAACTACTTAGCAGAAAAAGGCGCAGTTACAGGTA
ACGAGAAAGGAATGTTCGAGCCTGGAAAGGAAATCACTCGTGCAGAAGCAGCTACAATGATGGCTAAAATCTTAAACTTG
CCAATCGATAAAAATGCTAAACCATCTTACGCTGATTCTCAAAAACATTGGGCTACTCCAATCATCGCAGCTGTAGAAAA
AGCTGGTGTTGTTAAAGGTACAGGCAATGGATTTGAGCCAGACGGAAAAATCGACCGCGTGTCAATGGCATCTCTTCTTG
TAGAAGCTTACAAATTAGAGTCTAAAGTAAACGGTACTCCAGCAACTAAATTCAAAGATTTAGAAACATTAAATTGGGGT
AAAGAAAAAGCTAATATCTTAGTTGAATTGGGTATTTCTGTTGGTACTGGTGACAAATGGGAACCTAAGAAAATTCTAAC
TAAAGCAGAAGCAGCTCAATTTATAAAAAAGGCAGATTCTCTTAAAATAGGTAACCCTTTAGTAGAACAGGTAATTATTA
TTGATCCGGGCCATGGAGGAACAGACCCTGGGAAGGCAACGCAAGGATTACATGAAAGTGATATTGTATTAGATACATCT
AAAAGATTACAAAGTTTAATAGAGAAACAAACTCCATTCCGCGTGATGTTAACACGTGAAACAGATATTCGCCTTGATGA
AAAACAAGGAGAAGACCTTAAAAAGCGTGTGGATTTCGCAAAGGAACATAATGGAGATATTTTTGTAAGTATCCATGCAA
ATGCTTCTCAAAAACATGATGGATATGGAACAGAAACTTTTTATTATAAAGGATCTGAAAAGAAAGAATTAACTGAACGT
GAAAAAGATAGTTATATGTTAGCTAATAAAATACAAAAGAGATTAGTTAAAGCTCTAGATACAAGAGATCGTGGCGTAAA
ACCAGAAGATTTTTATGTACTAAGAGAAAATGAAATGCCAGCAGTACTAACGGAGCTAGCATTCTTAGATAATAGTACGG
ATTATGAGAAGTTAGCTTCAGAATCTGGAAGACAAATAGCTGCTGAGGCAATTTATGAGGGGATTTTAGATTATTATGAG
TGGAAAGGCTTTGATGTTTCTAAATCCCATTTAACAAAATAA

Upstream 100 bases:

>100_bases
AATACAAAATTATACAAATTTCAATTGACTATTACTAATTTTCAGAACTATAATTAGTCTGTTATATAAATACATTGTAC
TTGAGAGGGGAATATATTCT

Downstream 100 bases:

>100_bases
TAATGTTGATTACTAAGCCCATCGCCTTTATATAGATGGGCTTTTTATGTGATTTTATGTAATTTTGTATCGGAATAAAA
AGATATATCGTATTATGCTG

Product: S-layer protein and N-acetylmuramoyl-L-alanine amidase fusion protein

Products: NA

Alternate protein names: Surface array protein; Surface layer protein [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MKNRIIAAGVIAASILSYSSSSFAQTKTFPDVQAGHWAEDSINYLAEKGAVTGNEKGMFEPGKEITRAEAATMMAKILNL
PIDKNAKPSYADSQKHWATPIIAAVEKAGVVKGTGNGFEPDGKIDRVSMASLLVEAYKLESKVNGTPATKFKDLETLNWG
KEKANILVELGISVGTGDKWEPKKILTKAEAAQFIKKADSLKIGNPLVEQVIIIDPGHGGTDPGKATQGLHESDIVLDTS
KRLQSLIEKQTPFRVMLTRETDIRLDEKQGEDLKKRVDFAKEHNGDIFVSIHANASQKHDGYGTETFYYKGSEKKELTER
EKDSYMLANKIQKRLVKALDTRDRGVKPEDFYVLRENEMPAVLTELAFLDNSTDYEKLASESGRQIAAEAIYEGILDYYE
WKGFDVSKSHLTK

Sequences:

>Translated_413_residues
MKNRIIAAGVIAASILSYSSSSFAQTKTFPDVQAGHWAEDSINYLAEKGAVTGNEKGMFEPGKEITRAEAATMMAKILNL
PIDKNAKPSYADSQKHWATPIIAAVEKAGVVKGTGNGFEPDGKIDRVSMASLLVEAYKLESKVNGTPATKFKDLETLNWG
KEKANILVELGISVGTGDKWEPKKILTKAEAAQFIKKADSLKIGNPLVEQVIIIDPGHGGTDPGKATQGLHESDIVLDTS
KRLQSLIEKQTPFRVMLTRETDIRLDEKQGEDLKKRVDFAKEHNGDIFVSIHANASQKHDGYGTETFYYKGSEKKELTER
EKDSYMLANKIQKRLVKALDTRDRGVKPEDFYVLRENEMPAVLTELAFLDNSTDYEKLASESGRQIAAEAIYEGILDYYE
WKGFDVSKSHLTK
>Mature_413_residues
MKNRIIAAGVIAASILSYSSSSFAQTKTFPDVQAGHWAEDSINYLAEKGAVTGNEKGMFEPGKEITRAEAATMMAKILNL
PIDKNAKPSYADSQKHWATPIIAAVEKAGVVKGTGNGFEPDGKIDRVSMASLLVEAYKLESKVNGTPATKFKDLETLNWG
KEKANILVELGISVGTGDKWEPKKILTKAEAAQFIKKADSLKIGNPLVEQVIIIDPGHGGTDPGKATQGLHESDIVLDTS
KRLQSLIEKQTPFRVMLTRETDIRLDEKQGEDLKKRVDFAKEHNGDIFVSIHANASQKHDGYGTETFYYKGSEKKELTER
EKDSYMLANKIQKRLVKALDTRDRGVKPEDFYVLRENEMPAVLTELAFLDNSTDYEKLASESGRQIAAEAIYEGILDYYE
WKGFDVSKSHLTK

Specific function: The S-layer is a paracrystalline mono-layered assembly of proteins which coat the surface of bacteria [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall, S-layer [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 SLH (S-layer homology) domains [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=239, Percent_Identity=28.0334728033473, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87082163, Length=225, Percent_Identity=29.7777777777778, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003343
- InterPro:   IPR008964
- InterPro:   IPR001119 [H]

Pfam domain/function: PF02368 Big_2; PF00395 SLH [H]

EC number: 3.5.1.28

Molecular weight: Translated: 45752; Mature: 45752

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRIIAAGVIAASILSYSSSSFAQTKTFPDVQAGHWAEDSINYLAEKGAVTGNEKGMFE
CCCCEEEHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCC
PGKEITRAEAATMMAKILNLPIDKNAKPSYADSQKHWATPIIAAVEKAGVVKGTGNGFEP
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCEECCCCCCCC
DGKIDRVSMASLLVEAYKLESKVNGTPATKFKDLETLNWGKEKANILVELGISVGTGDKW
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCHHCCEEEEECCEECCCCCC
EPKKILTKAEAAQFIKKADSLKIGNPLVEQVIIIDPGHGGTDPGKATQGLHESDIVLDTS
CHHHHHHHHHHHHHHHHHCCCCCCCHHHCEEEEEECCCCCCCCCHHHCCCCCCCEEEECH
KRLQSLIEKQTPFRVMLTRETDIRLDEKQGEDLKKRVDFAKEHNGDIFVSIHANASQKHD
HHHHHHHHHCCCEEEEEEECCCCEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYGTETFYYKGSEKKELTEREKDSYMLANKIQKRLVKALDTRDRGVKPEDFYVLRENEMP
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
AVLTELAFLDNSTDYEKLASESGRQIAAEAIYEGILDYYEWKGFDVSKSHLTK
HHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCC
>Mature Secondary Structure
MKNRIIAAGVIAASILSYSSSSFAQTKTFPDVQAGHWAEDSINYLAEKGAVTGNEKGMFE
CCCCEEEHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCC
PGKEITRAEAATMMAKILNLPIDKNAKPSYADSQKHWATPIIAAVEKAGVVKGTGNGFEP
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCEECCCCCCCC
DGKIDRVSMASLLVEAYKLESKVNGTPATKFKDLETLNWGKEKANILVELGISVGTGDKW
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCHHCCEEEEECCEECCCCCC
EPKKILTKAEAAQFIKKADSLKIGNPLVEQVIIIDPGHGGTDPGKATQGLHESDIVLDTS
CHHHHHHHHHHHHHHHHHCCCCCCCHHHCEEEEEECCCCCCCCCHHHCCCCCCCEEEECH
KRLQSLIEKQTPFRVMLTRETDIRLDEKQGEDLKKRVDFAKEHNGDIFVSIHANASQKHD
HHHHHHHHHCCCEEEEEEECCCCEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYGTETFYYKGSEKKELTEREKDSYMLANKIQKRLVKALDTRDRGVKPEDFYVLRENEMP
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
AVLTELAFLDNSTDYEKLASESGRQIAAEAIYEGILDYYEWKGFDVSKSHLTK
HHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836294; 12721629 [H]