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The map label for this gene is livJ [C]

Identifier: 52143485

GI number: 52143485

Start: 1873773

End: 1874990

Strand: Direct

Name: livJ [C]

Synonym: BCZK1749

Alternate gene names: 52143485

Gene position: 1873773-1874990 (Clockwise)

Preceding gene: 52143487

Following gene: 52143484

Centisome position: 35.35

GC content: 37.77

Gene sequence:

>1218_bases
ATGTCGATGAAAAAACGTAAATGGCTAAGAATGATGGCTACTGGTTGTGTTTTAGGTTCGTTATTCATAACGGCAGCTTG
TTCAGGAAAGAAAACAAGTACAGAGGATGAAAAGACGATTAAGGTAGGGGTTCTTGCATCCTTAACAGGTCCATTAGAAT
CGTATGGAAAACAAACAATGAACGGATTTGAATTAGGGTTAGACTATGCAACTGGTGGAACTGGGAAAGTGGAAGGGAAG
AAGATTAAGTTTGTTGTAGAAGATACGGAAACGAAAGCGGATGTAGCAGTTAAAAAAGCTACGAAATTATTAGAAGAAGA
GAAAGTTGATTTTTTAGTCGGATCGTCTAGTTCAAGTGATACATTAGCGGTTTTACCACTAGCGGAAGAGTACGAAAAAA
TAATGGTTGTAGAACCAGCAGTGGCGGATAGTATTACCGGCAAGAACTGGAATAAATATATTTTTAGAACAGGTAGAAAT
TCCTCTCAAGATGCAGTCGCTGGTGCTGCAGCAATTGCTAAAAAAGATGTGAAAATAGCAACGTTAGCACAAGACAATGC
ATACGGCCGTGAAGGAATTGCGGCATTTAAAGCTGGAGCGAAGAAATTAGGTGCAAATATCGTAAACGAGCAATATGCTG
ATACAAATTCGACTGACTTCACTGCAAATATTCAAAATATCATTAGCTCAAAACCAGATTATTTATTTATCGTTTGGGCA
GGGGCAAACTCACCTTGGAAACAGTTAAAAGATATGAATGTGGAAGCGCAAGGTATTAAAATTTCTACAGGTGCACCAGA
TATACCGGCATTAAAAACGATGGATGCGTTAGTAGGAATGCAAGGTTTTTCTGTTTATTATCATACACTCCCGAAAAATA
AGGTGAATGATTGGTTAGTGGAAGAACATAAAAAACGATTTAATGGTGCGGTGCCTGATTTATTTACAGCAGGAGGAATG
TCAGCGGCAATTTCAATTGTAGAAGCGTTAAAGAAAACAAAAGGTGATACAGATGTAGATACATTAATTAAGAAAATGGA
AGGAATGGAATTTGATACACCGAAAGGAAAGATGAAGTTTAGAGAAAAGGATCATCAAGCGATGCAGACACTTTATTCTA
TCACATTGAAAAAGCAAGATGGTGTTGATTATCCGGTGCCAGTATTAGAGCGAGAATTAACGATGAAAGAGACAGAGCCA
CCAGTTCAAAGTAAATAG

Upstream 100 bases:

>100_bases
ACAGATTTGTTTGTATGATTCAAAGAAAAAGTCATAATTACGGGTGGATAACTTTGGCGAGATTGATTCTATCAGAAAGT
AAAGTATGGGGAGGATCACA

Downstream 100 bases:

>100_bases
ACTGAATTTTCTGTTGTTTTTATATAAAGAGGGGACACCCTCTTTATACGTATTTCTTTCATACTTAAAGCTTGAAAGGG
AGGGATATCGTGACACATTT

Product: branched-chain amino acid ABC transporter, substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSMKKRKWLRMMATGCVLGSLFITAACSGKKTSTEDEKTIKVGVLASLTGPLESYGKQTMNGFELGLDYATGGTGKVEGK
KIKFVVEDTETKADVAVKKATKLLEEEKVDFLVGSSSSSDTLAVLPLAEEYEKIMVVEPAVADSITGKNWNKYIFRTGRN
SSQDAVAGAAAIAKKDVKIATLAQDNAYGREGIAAFKAGAKKLGANIVNEQYADTNSTDFTANIQNIISSKPDYLFIVWA
GANSPWKQLKDMNVEAQGIKISTGAPDIPALKTMDALVGMQGFSVYYHTLPKNKVNDWLVEEHKKRFNGAVPDLFTAGGM
SAAISIVEALKKTKGDTDVDTLIKKMEGMEFDTPKGKMKFREKDHQAMQTLYSITLKKQDGVDYPVPVLERELTMKETEP
PVQSK

Sequences:

>Translated_405_residues
MSMKKRKWLRMMATGCVLGSLFITAACSGKKTSTEDEKTIKVGVLASLTGPLESYGKQTMNGFELGLDYATGGTGKVEGK
KIKFVVEDTETKADVAVKKATKLLEEEKVDFLVGSSSSSDTLAVLPLAEEYEKIMVVEPAVADSITGKNWNKYIFRTGRN
SSQDAVAGAAAIAKKDVKIATLAQDNAYGREGIAAFKAGAKKLGANIVNEQYADTNSTDFTANIQNIISSKPDYLFIVWA
GANSPWKQLKDMNVEAQGIKISTGAPDIPALKTMDALVGMQGFSVYYHTLPKNKVNDWLVEEHKKRFNGAVPDLFTAGGM
SAAISIVEALKKTKGDTDVDTLIKKMEGMEFDTPKGKMKFREKDHQAMQTLYSITLKKQDGVDYPVPVLERELTMKETEP
PVQSK
>Mature_404_residues
SMKKRKWLRMMATGCVLGSLFITAACSGKKTSTEDEKTIKVGVLASLTGPLESYGKQTMNGFELGLDYATGGTGKVEGKK
IKFVVEDTETKADVAVKKATKLLEEEKVDFLVGSSSSSDTLAVLPLAEEYEKIMVVEPAVADSITGKNWNKYIFRTGRNS
SQDAVAGAAAIAKKDVKIATLAQDNAYGREGIAAFKAGAKKLGANIVNEQYADTNSTDFTANIQNIISSKPDYLFIVWAG
ANSPWKQLKDMNVEAQGIKISTGAPDIPALKTMDALVGMQGFSVYYHTLPKNKVNDWLVEEHKKRFNGAVPDLFTAGGMS
AAISIVEALKKTKGDTDVDTLIKKMEGMEFDTPKGKMKFREKDHQAMQTLYSITLKKQDGVDYPVPVLERELTMKETEPP
VQSK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 44142; Mature: 44011

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMKKRKWLRMMATGCVLGSLFITAACSGKKTSTEDEKTIKVGVLASLTGPLESYGKQTM
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHCCCHHHHHHHHHC
NGFELGLDYATGGTGKVEGKKIKFVVEDTETKADVAVKKATKLLEEEKVDFLVGSSSSSD
CHHHEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCC
TLAVLPLAEEYEKIMVVEPAVADSITGKNWNKYIFRTGRNSSQDAVAGAAAIAKKDVKIA
CEEEEECHHCCCEEEEECCHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEE
TLAQDNAYGREGIAAFKAGAKKLGANIVNEQYADTNSTDFTANIQNIISSKPDYLFIVWA
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHCCCCCEEEEEEE
GANSPWKQLKDMNVEAQGIKISTGAPDIPALKTMDALVGMQGFSVYYHTLPKNKVNDWLV
CCCCHHHHHHCCCCEECCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
EEHKKRFNGAVPDLFTAGGMSAAISIVEALKKTKGDTDVDTLIKKMEGMEFDTPKGKMKF
HHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCHH
REKDHQAMQTLYSITLKKQDGVDYPVPVLERELTMKETEPPVQSK
HHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SMKKRKWLRMMATGCVLGSLFITAACSGKKTSTEDEKTIKVGVLASLTGPLESYGKQTM
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHCCCHHHHHHHHHC
NGFELGLDYATGGTGKVEGKKIKFVVEDTETKADVAVKKATKLLEEEKVDFLVGSSSSSD
CHHHEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCC
TLAVLPLAEEYEKIMVVEPAVADSITGKNWNKYIFRTGRNSSQDAVAGAAAIAKKDVKIA
CEEEEECHHCCCEEEEECCHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEE
TLAQDNAYGREGIAAFKAGAKKLGANIVNEQYADTNSTDFTANIQNIISSKPDYLFIVWA
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHCCCCCEEEEEEE
GANSPWKQLKDMNVEAQGIKISTGAPDIPALKTMDALVGMQGFSVYYHTLPKNKVNDWLV
CCCCHHHHHHCCCCEECCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
EEHKKRFNGAVPDLFTAGGMSAAISIVEALKKTKGDTDVDTLIKKMEGMEFDTPKGKMKF
HHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCHH
REKDHQAMQTLYSITLKKQDGVDYPVPVLERELTMKETEPPVQSK
HHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA