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The map label for this gene is prpD [H]

Identifier: 52143126

GI number: 52143126

Start: 2232035

End: 2233471

Strand: Direct

Name: prpD [H]

Synonym: BCZK2112

Alternate gene names: 52143126

Gene position: 2232035-2233471 (Clockwise)

Preceding gene: 52143128

Following gene: 52143124

Centisome position: 42.11

GC content: 36.6

Gene sequence:

>1437_bases
ATGATTAAAACAAATGAAATTAAGCAAAAAGATGCATTATTAGAAGAAATTACGGATTATGTATTAAATAAAGAGGTAAC
AAGTGCAGAAGCATTCAGTACTGCTCGTTATGTATTACTTGATACTCTTGGATGCGGAATTTTAGCACTACAATATCCAG
AGTGTACGAAATTGCTAGGACCAGTTGTACCAGGAACAATCGTGCCAAACGGTACACGTGTACCAGGAACTTCTTATGTA
CTGGATCCAGTGAAAGGTGCATTTAATATCGGATGTATGATCCGTTGGTTAGACTATAACGATACTTGGCTTGCAGCAGA
ATGGGGACATCCATCTGATAACTTAGGCGGAATTTTAGCAGTTGCAGATTATATTAGCCGTGTTCGTATATCAGAAGGAA
AAGAAGCGTTAAAAGTACGTGAAGTATTAGAAATGATGATTAAAGCACATGAAATTCAAGGTGTACTTGCTTTAGAAAAC
AGCTTAAACCGCGTTGGCCTTGACCACGTATTATACGTAAAAGTAGCAACAACTGCTGTAGTTGCGAAAATGCTTGGTGG
AACACGTGAAGAAATCTTTAATGCATTATCACATGCATGGATTGATAATTCTAGTCTTCGTACATATCGTCACGCTCCAA
ATACTGGATCACGTAAATCATGGGCAGCAGGTGATGCAACAAGCCGCGGTGTTCACCTTGCAATGACTGCTTTAAAAGGT
GAAATGGGTTATCCAACAGCATTATCTGCACCGGGCTGGGGATTCCAAGATGTATTATTTAATAAACAAGAATTAAAGTT
AGCTAGACGATTAGAGTCTTATGTAATGGAAAATGTATTATTTAAAGTTTCATATCCAGCAGAATTCCATGCACAAACAG
CTGCAGAATGTGCTGTAAAATTACATCCCGAAATTAAAGAAAGATTAGATGAAATTGACCGTATTACAATTACAACTCAT
GAATCAGCAATTCGTATTATTGATAAAGAAGGTCCATTAAATAACCCAGCTGATCGTGATCATTGTTTACAATATATTAC
GGCAATTGGTTTATTAAAGGGAGATATCGTTGCGGATGATTATGAGGATGCAGTAGCAAATGATCCACGTGTAGATGAAT
TACGTAATAAGATGGTTGTTGTTGAAAACAAACAGTACAGTTTAGATTACCTTGACCCGAACAAGCGCTCAATCGCCAAC
GCTGTTCAAGTTCATTTCAAGGATGGAACTGTAACAGAAAACGTGGAATGTGAATATCCACTTGGTCACCGTTTCCGTAG
AGACGAAGCAATTCCAAAAGTTGTTCAAAAATTCACTGCAAATATGGCAGGTCATTATTCTAGTAAGCAACAAGAACAAA
TTCATGAAGTTTGTTTAAATGAAGAGAAACTAGAAAATATGAATGTAAACGAATTTGTAGATCTATTCTTAATTTAA

Upstream 100 bases:

>100_bases
AAGAACAACTGGGGAGTTGTTAGCGCCGCCCGCCAGATGGGTGTTTGACCGACACCCATCATTAATAATAACGAATTTTC
GACAGAAAGGGAAGGATAGC

Downstream 100 bases:

>100_bases
TAATAGGGGGAATTTAGAATGGCTTGGGTTGTGAATAAACAGTCAACACAAGAGGAGCTTGCGAATCGCTTCCGCGCTTT
AGTAGAAGCAAATGAAATTT

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MIKTNEIKQKDALLEEITDYVLNKEVTSAEAFSTARYVLLDTLGCGILALQYPECTKLLGPVVPGTIVPNGTRVPGTSYV
LDPVKGAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGGILAVADYISRVRISEGKEALKVREVLEMMIKAHEIQGVLALEN
SLNRVGLDHVLYVKVATTAVVAKMLGGTREEIFNALSHAWIDNSSLRTYRHAPNTGSRKSWAAGDATSRGVHLAMTALKG
EMGYPTALSAPGWGFQDVLFNKQELKLARRLESYVMENVLFKVSYPAEFHAQTAAECAVKLHPEIKERLDEIDRITITTH
ESAIRIIDKEGPLNNPADRDHCLQYITAIGLLKGDIVADDYEDAVANDPRVDELRNKMVVVENKQYSLDYLDPNKRSIAN
AVQVHFKDGTVTENVECEYPLGHRFRRDEAIPKVVQKFTANMAGHYSSKQQEQIHEVCLNEEKLENMNVNEFVDLFLI

Sequences:

>Translated_478_residues
MIKTNEIKQKDALLEEITDYVLNKEVTSAEAFSTARYVLLDTLGCGILALQYPECTKLLGPVVPGTIVPNGTRVPGTSYV
LDPVKGAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGGILAVADYISRVRISEGKEALKVREVLEMMIKAHEIQGVLALEN
SLNRVGLDHVLYVKVATTAVVAKMLGGTREEIFNALSHAWIDNSSLRTYRHAPNTGSRKSWAAGDATSRGVHLAMTALKG
EMGYPTALSAPGWGFQDVLFNKQELKLARRLESYVMENVLFKVSYPAEFHAQTAAECAVKLHPEIKERLDEIDRITITTH
ESAIRIIDKEGPLNNPADRDHCLQYITAIGLLKGDIVADDYEDAVANDPRVDELRNKMVVVENKQYSLDYLDPNKRSIAN
AVQVHFKDGTVTENVECEYPLGHRFRRDEAIPKVVQKFTANMAGHYSSKQQEQIHEVCLNEEKLENMNVNEFVDLFLI
>Mature_478_residues
MIKTNEIKQKDALLEEITDYVLNKEVTSAEAFSTARYVLLDTLGCGILALQYPECTKLLGPVVPGTIVPNGTRVPGTSYV
LDPVKGAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGGILAVADYISRVRISEGKEALKVREVLEMMIKAHEIQGVLALEN
SLNRVGLDHVLYVKVATTAVVAKMLGGTREEIFNALSHAWIDNSSLRTYRHAPNTGSRKSWAAGDATSRGVHLAMTALKG
EMGYPTALSAPGWGFQDVLFNKQELKLARRLESYVMENVLFKVSYPAEFHAQTAAECAVKLHPEIKERLDEIDRITITTH
ESAIRIIDKEGPLNNPADRDHCLQYITAIGLLKGDIVADDYEDAVANDPRVDELRNKMVVVENKQYSLDYLDPNKRSIAN
AVQVHFKDGTVTENVECEYPLGHRFRRDEAIPKVVQKFTANMAGHYSSKQQEQIHEVCLNEEKLENMNVNEFVDLFLI

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=480, Percent_Identity=61.25, Blast_Score=624, Evalue=1e-180,
Organism=Saccharomyces cerevisiae, GI6325258, Length=486, Percent_Identity=53.0864197530864, Blast_Score=524, Evalue=1e-149,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 53457; Mature: 53457

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTNEIKQKDALLEEITDYVLNKEVTSAEAFSTARYVLLDTLGCGILALQYPECTKLLG
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHC
PVVPGTIVPNGTRVPGTSYVLDPVKGAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGGILA
CCCCCCCCCCCCCCCCCCEEECCCCCCEEECEEEEEEECCCCEEEEECCCCCCCCCHHHH
VADYISRVRISEGKEALKVREVLEMMIKAHEIQGVLALENSLNRVGLDHVLYVKVATTAV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHCCCCCEEEEHHHHHHH
VAKMLGGTREEIFNALSHAWIDNSSLRTYRHAPNTGSRKSWAAGDATSRGVHLAMTALKG
HHHHHCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCC
EMGYPTALSAPGWGFQDVLFNKQELKLARRLESYVMENVLFKVSYPAEFHAQTAAECAVK
CCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHC
LHPEIKERLDEIDRITITTHESAIRIIDKEGPLNNPADRDHCLQYITAIGLLKGDIVADD
CCHHHHHHHHHHCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEECCC
YEDAVANDPRVDELRNKMVVVENKQYSLDYLDPNKRSIANAVQVHFKDGTVTENVECEYP
CHHHHCCCCCHHHHCCCEEEEECCCEEEECCCCCHHHHHHEEEEEECCCCCCCCCEECCC
LGHRFRRDEAIPKVVQKFTANMAGHYSSKQQEQIHEVCLNEEKLENMNVNEFVDLFLI
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MIKTNEIKQKDALLEEITDYVLNKEVTSAEAFSTARYVLLDTLGCGILALQYPECTKLLG
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHC
PVVPGTIVPNGTRVPGTSYVLDPVKGAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGGILA
CCCCCCCCCCCCCCCCCCEEECCCCCCEEECEEEEEEECCCCEEEEECCCCCCCCCHHHH
VADYISRVRISEGKEALKVREVLEMMIKAHEIQGVLALENSLNRVGLDHVLYVKVATTAV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHCCCCCEEEEHHHHHHH
VAKMLGGTREEIFNALSHAWIDNSSLRTYRHAPNTGSRKSWAAGDATSRGVHLAMTALKG
HHHHHCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCC
EMGYPTALSAPGWGFQDVLFNKQELKLARRLESYVMENVLFKVSYPAEFHAQTAAECAVK
CCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHC
LHPEIKERLDEIDRITITTHESAIRIIDKEGPLNNPADRDHCLQYITAIGLLKGDIVADD
CCHHHHHHHHHHCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEECCC
YEDAVANDPRVDELRNKMVVVENKQYSLDYLDPNKRSIANAVQVHFKDGTVTENVECEYP
CHHHHCCCCCHHHHCCCEEEEECCCEEEECCCCCHHHHHHEEEEEECCCCCCCCCEECCC
LGHRFRRDEAIPKVVQKFTANMAGHYSSKQQEQIHEVCLNEEKLENMNVNEFVDLFLI
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8759838 [H]