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The map label for this gene is prpB [H]

Identifier: 52143124

GI number: 52143124

Start: 2233490

End: 2234398

Strand: Direct

Name: prpB [H]

Synonym: BCZK2113

Alternate gene names: 52143124

Gene position: 2233490-2234398 (Clockwise)

Preceding gene: 52143126

Following gene: 52143125

Centisome position: 42.13

GC content: 39.38

Gene sequence:

>909_bases
ATGGCTTGGGTTGTGAATAAACAGTCAACACAAGAGGAGCTTGCGAATCGCTTCCGCGCTTTAGTAGAAGCAAATGAAAT
TTTGCAAATTCCAGGTGCTCATGACGCAATGGCAGCTTTAGTTGCGAGAAATACAGGATTTTCGGCTCTTTATTTATCAG
GAGCTGCCTATACTGCAAGTAAAGGATTACCTGATTTAGGAATCGTGACGTCTACTGAAGTAGCAGAGAGAGCAAGAGAT
CTAGTAAGAGCGACAGATTTACCAGTTCTTGTTGATATTGATACAGGATTTGGCGGTGTCCTAAACGTAGCGAGAACAGC
TGTAGAAATGGTGGAAGCGAAAGTAGCAGCTGTTCAAATTGAAGATCAACAATTACCAAAAAAATGTGGACATTTAAATG
GTAAGAAACTCGTTACGACAGAAGAATTAGTTCAAAAGATTAAAGCGATTAAAGAAGTTGCACCAAGCTTATATATTGTA
GCGCGCACAGATGCTCGCGGCGTAGAAGGCTTGGATGAAGCGATTGAAAGAGCGAATGCTTATGTAAAAGCAGGTGCAGA
TGCGATATTCCCTGAAGCACTTCAATCAGAAGAGGAGTTCCGTCTATTCAATAGTAAAGTAAATGCACCTTTACTTGCGA
ATATGACTGAATTTGGGAAAACGCCGTATTATAGTGCAGAGGAATTTGCGAATATGGGCTTCCAAATGGTTATTTATCCT
GTAACTTCACTTCGCGTTGCAGCGAAAGCGTATGAGAATGTGTTTACTTTAATTAAGGAAACTGGTTCTCAAAAAGATGC
ACTTTCAAATATGCAAACGAGAAGTGAGTTATATGAAACAATTTCATATCATGACTTTGAAGAACTAGATACTGGTATTG
CAAAAACAGTATTATCAGAGGATCAATAG

Upstream 100 bases:

>100_bases
ACAAATTCATGAAGTTTGTTTAAATGAAGAGAAACTAGAAAATATGAATGTAAACGAATTTGTAGATCTATTCTTAATTT
AATAATAGGGGGAATTTAGA

Downstream 100 bases:

>100_bases
ATAAAACAACTAAGGCGATGAGAATACATCTTTAGCAAGACGAACTGCTTGATGTGAAAATTCATCGCCTTCTGTTTTCA
AAAATAAGATATTCCACTCA

Product: carboxyvinyl-carboxyphosphonate phosphorylmutase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MAWVVNKQSTQEELANRFRALVEANEILQIPGAHDAMAALVARNTGFSALYLSGAAYTASKGLPDLGIVTSTEVAERARD
LVRATDLPVLVDIDTGFGGVLNVARTAVEMVEAKVAAVQIEDQQLPKKCGHLNGKKLVTTEELVQKIKAIKEVAPSLYIV
ARTDARGVEGLDEAIERANAYVKAGADAIFPEALQSEEEFRLFNSKVNAPLLANMTEFGKTPYYSAEEFANMGFQMVIYP
VTSLRVAAKAYENVFTLIKETGSQKDALSNMQTRSELYETISYHDFEELDTGIAKTVLSEDQ

Sequences:

>Translated_302_residues
MAWVVNKQSTQEELANRFRALVEANEILQIPGAHDAMAALVARNTGFSALYLSGAAYTASKGLPDLGIVTSTEVAERARD
LVRATDLPVLVDIDTGFGGVLNVARTAVEMVEAKVAAVQIEDQQLPKKCGHLNGKKLVTTEELVQKIKAIKEVAPSLYIV
ARTDARGVEGLDEAIERANAYVKAGADAIFPEALQSEEEFRLFNSKVNAPLLANMTEFGKTPYYSAEEFANMGFQMVIYP
VTSLRVAAKAYENVFTLIKETGSQKDALSNMQTRSELYETISYHDFEELDTGIAKTVLSEDQ
>Mature_301_residues
AWVVNKQSTQEELANRFRALVEANEILQIPGAHDAMAALVARNTGFSALYLSGAAYTASKGLPDLGIVTSTEVAERARDL
VRATDLPVLVDIDTGFGGVLNVARTAVEMVEAKVAAVQIEDQQLPKKCGHLNGKKLVTTEELVQKIKAIKEVAPSLYIVA
RTDARGVEGLDEAIERANAYVKAGADAIFPEALQSEEEFRLFNSKVNAPLLANMTEFGKTPYYSAEEFANMGFQMVIYPV
TSLRVAAKAYENVFTLIKETGSQKDALSNMQTRSELYETISYHDFEELDTGIAKTVLSEDQ

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=284, Percent_Identity=44.7183098591549, Blast_Score=238, Evalue=4e-64,
Organism=Escherichia coli, GI1790445, Length=259, Percent_Identity=29.3436293436293, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71982926, Length=200, Percent_Identity=30.5, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17561814, Length=200, Percent_Identity=30.5, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32990; Mature: 32859

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWVVNKQSTQEELANRFRALVEANEILQIPGAHDAMAALVARNTGFSALYLSGAAYTAS
CCEECCCCCHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCEEEEECCCHHHHC
KGLPDLGIVTSTEVAERARDLVRATDLPVLVDIDTGFGGVLNVARTAVEMVEAKVAAVQI
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEE
EDQQLPKKCGHLNGKKLVTTEELVQKIKAIKEVAPSLYIVARTDARGVEGLDEAIERANA
CHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHH
YVKAGADAIFPEALQSEEEFRLFNSKVNAPLLANMTEFGKTPYYSAEEFANMGFQMVIYP
HHHCCCHHHCHHHHCCHHHHHHHHHCCCCCHHHCHHHHCCCCCCCHHHHHHCCCEEEEEE
VTSLRVAAKAYENVFTLIKETGSQKDALSNMQTRSELYETISYHDFEELDTGIAKTVLSE
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
DQ
CC
>Mature Secondary Structure 
AWVVNKQSTQEELANRFRALVEANEILQIPGAHDAMAALVARNTGFSALYLSGAAYTAS
CEECCCCCHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCEEEEECCCHHHHC
KGLPDLGIVTSTEVAERARDLVRATDLPVLVDIDTGFGGVLNVARTAVEMVEAKVAAVQI
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEE
EDQQLPKKCGHLNGKKLVTTEELVQKIKAIKEVAPSLYIVARTDARGVEGLDEAIERANA
CHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHH
YVKAGADAIFPEALQSEEEFRLFNSKVNAPLLANMTEFGKTPYYSAEEFANMGFQMVIYP
HHHCCCHHHCHHHHCCHHHHHHHHHCCCCCHHHCHHHHCCCCCCCHHHHHHCCCEEEEEE
VTSLRVAAKAYENVFTLIKETGSQKDALSNMQTRSELYETISYHDFEELDTGIAKTVLSE
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
DQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]