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The map label for this gene is entE [H]

Identifier: 52143109

GI number: 52143109

Start: 2252232

End: 2253848

Strand: Direct

Name: entE [H]

Synonym: BCZK2129

Alternate gene names: 52143109

Gene position: 2252232-2253848 (Clockwise)

Preceding gene: 52143110

Following gene: 52143108

Centisome position: 42.49

GC content: 38.28

Gene sequence:

>1617_bases
ATGTTAACAGGTTATACAAAATGGCCAAAAGAATTTGCCGATCGCTATCGAGAGGAAGGGTGTTGGCTTGGGGAAACATT
TGGCGGAGTGTTAAGAGAGCGTGCTGAAAAATATGGAGATCAAATTGCGGTTGTAAGTGGTAATACGTATATAACATATA
GTGAGCTTGATAAAAAAGTAGATCGCTTAGCGTCAGGTTTACTGAATTTAGGAATAAAGCCAGAAGATCGAGTTGTAATC
CAACTACCTAACGTTATCGAGTTTTTTGAAATGTGTTTTGCGCTATTTCGAATTGGAGCACTTCCTGTTTTTGCATTACC
TTCACATCGCAGTAGTGAAATTAGTTATTTTTGTGAGTTTGGTGAGGCGAGTGCGTACGTTATTTCAGATAAGGCTCTTG
GTTTTGATTATCGAAAACTAGCAAGAGAAGTGCAAGAGAAAGTACCAACTTTACAACATGTAATTGTAGTGGGAGAAGAA
GAAGAGTTTGTGAACATAAGTGATTTATATATAGATCCCGTTCCATTGCCAGAAGTTCAGCCAAGTGATGTTGCGTTTCT
CCAATTATCAGGAGGGACAACAGGACTTTCTAAATTAATTCCTAGAACGCATGATGACTATATTTATAGTTTACGTGTTA
GCGCTGAAATTTGTAATTTAAGTGCAGAGAGCGTCTATATGGCAGTTCTTCCAGTAGCACATAATTACCCAATGAGTTCT
CCTGGAACATTTGGAACTTTTTATGCGGGTGGAAAAGTTGTGCTGGCAACTGGGGGTAGTCCGGATGAGACATTTGCACT
TATCGAAAAAGAAAAAGTTACGATTACTGCTCTCGTACCGCCATTAGCAATGATATGGCTTGACGCTGCATCTTTACGTA
ATAACGATTTATCAAGCTTAGAAGTGATTCAAGTAGGTGGTGCGAAATTTAGTGTTGAAGTTGCAAAACGTATACGTCCT
ACATTTGGATGTACATTACAGCAAGTGTTCGGTATGGCAGAAGGATTAGTGAACTATACAAGATTAGATGATCCGGAAGA
AATTATAATTCATACACAAGGTAGACCAATGTCTGCACTTGATGAAGTACGAGTTGTTGACGAATATGATAACGATGTAA
AACCTGGTGAAGTAGGTAGTTTATTAACACGGGGTCCATATACAATTCGTGGATACTATAAAGCAGAAGAGCATAATGCA
CGATCATTTACAAATGATGGATTTTATCGCACAGGAGATCTTGTAAAAGTAAACGAACAAGGTTACATCATTGTAGAAGG
AAGAGATAAAGATCAAATTAATCGTGGTGGTGAGAAAGTCGCTGCGGAAGAAGTTGAAAATCATCTATTAGCACATGATG
CAGTACATGATGTAGCGATTGTATCTATGCCTGACGATTATTTAGGAGAGCGTACTTGTGCATTTGTAATAGCTCGCGGA
CAAGCTCCGGCAGTAAGTGAATTAAAAAGGTTCTTAAGAGAACGCGGTATAGCAGCTTATAAGATTCCAGATCGAATTGA
ATTTATTGAATCATTCCCGCAAACAGGAGTCGGAAAAGTCAGTAAAAAGGAGCTACGTAAAGTCATTGCTGAAAAACTTA
TTACAGTAAAACAATAA

Upstream 100 bases:

>100_bases
AGAAGATGAATTAGCAGAAACAACAGCTAAGTTTCAAACAATGTTGAAAGCTTTAGGGTTAAATGATAGTTCACTTAATG
AAAAGTAGGGGGCAGAAAGA

Downstream 100 bases:

>100_bases
TAAAAACCGGAAGGAAGTGATTAGATGGCTATTCCATCTATTTCAGTATACAAAATGCCAATTGAATCAGAACTACCAAA
AAATAAAGTGAATTGGACAC

Product: 2,3-dihydroxybenzoate-AMP ligase

Products: diphosphate; (2,3-dihydroxybenzoyl)adenylate

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MLTGYTKWPKEFADRYREEGCWLGETFGGVLRERAEKYGDQIAVVSGNTYITYSELDKKVDRLASGLLNLGIKPEDRVVI
QLPNVIEFFEMCFALFRIGALPVFALPSHRSSEISYFCEFGEASAYVISDKALGFDYRKLAREVQEKVPTLQHVIVVGEE
EEFVNISDLYIDPVPLPEVQPSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLSAESVYMAVLPVAHNYPMSS
PGTFGTFYAGGKVVLATGGSPDETFALIEKEKVTITALVPPLAMIWLDAASLRNNDLSSLEVIQVGGAKFSVEVAKRIRP
TFGCTLQQVFGMAEGLVNYTRLDDPEEIIIHTQGRPMSALDEVRVVDEYDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNA
RSFTNDGFYRTGDLVKVNEQGYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
QAPAVSELKRFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLITVKQ

Sequences:

>Translated_538_residues
MLTGYTKWPKEFADRYREEGCWLGETFGGVLRERAEKYGDQIAVVSGNTYITYSELDKKVDRLASGLLNLGIKPEDRVVI
QLPNVIEFFEMCFALFRIGALPVFALPSHRSSEISYFCEFGEASAYVISDKALGFDYRKLAREVQEKVPTLQHVIVVGEE
EEFVNISDLYIDPVPLPEVQPSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLSAESVYMAVLPVAHNYPMSS
PGTFGTFYAGGKVVLATGGSPDETFALIEKEKVTITALVPPLAMIWLDAASLRNNDLSSLEVIQVGGAKFSVEVAKRIRP
TFGCTLQQVFGMAEGLVNYTRLDDPEEIIIHTQGRPMSALDEVRVVDEYDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNA
RSFTNDGFYRTGDLVKVNEQGYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
QAPAVSELKRFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLITVKQ
>Mature_538_residues
MLTGYTKWPKEFADRYREEGCWLGETFGGVLRERAEKYGDQIAVVSGNTYITYSELDKKVDRLASGLLNLGIKPEDRVVI
QLPNVIEFFEMCFALFRIGALPVFALPSHRSSEISYFCEFGEASAYVISDKALGFDYRKLAREVQEKVPTLQHVIVVGEE
EEFVNISDLYIDPVPLPEVQPSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLSAESVYMAVLPVAHNYPMSS
PGTFGTFYAGGKVVLATGGSPDETFALIEKEKVTITALVPPLAMIWLDAASLRNNDLSSLEVIQVGGAKFSVEVAKRIRP
TFGCTLQQVFGMAEGLVNYTRLDDPEEIIIHTQGRPMSALDEVRVVDEYDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNA
RSFTNDGFYRTGDLVKVNEQGYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
QAPAVSELKRFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLITVKQ

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=548, Percent_Identity=24.8175182481752, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI187761345, Length=536, Percent_Identity=26.4925373134328, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI187761343, Length=536, Percent_Identity=26.4925373134328, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI28416953, Length=522, Percent_Identity=25.6704980842912, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI38505220, Length=499, Percent_Identity=23.8476953907816, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI42544132, Length=475, Percent_Identity=23.7894736842105, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI157311624, Length=492, Percent_Identity=24.1869918699187, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI157311622, Length=492, Percent_Identity=24.1869918699187, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI115511026, Length=506, Percent_Identity=24.3083003952569, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI122937307, Length=498, Percent_Identity=23.6947791164659, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI58082049, Length=499, Percent_Identity=23.6472945891784, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI8923896, Length=567, Percent_Identity=25.2204585537919, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI253970489, Length=126, Percent_Identity=34.9206349206349, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1786810, Length=541, Percent_Identity=48.6136783733826, Blast_Score=493, Evalue=1e-140,
Organism=Escherichia coli, GI145693145, Length=532, Percent_Identity=31.5789473684211, Blast_Score=246, Evalue=4e-66,
Organism=Escherichia coli, GI1788107, Length=539, Percent_Identity=27.8293135435993, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI221142682, Length=523, Percent_Identity=24.4741873804971, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1790505, Length=547, Percent_Identity=23.217550274223, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1789201, Length=356, Percent_Identity=25, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786529, Length=522, Percent_Identity=23.5632183908046, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786801, Length=457, Percent_Identity=22.1006564551422, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71994703, Length=552, Percent_Identity=27.7173913043478, Blast_Score=162, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI71994694, Length=552, Percent_Identity=27.7173913043478, Blast_Score=161, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI71994690, Length=552, Percent_Identity=27.7173913043478, Blast_Score=161, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17538037, Length=534, Percent_Identity=27.9026217228464, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17531443, Length=527, Percent_Identity=25.9962049335863, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17557194, Length=532, Percent_Identity=26.6917293233083, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17560308, Length=559, Percent_Identity=26.654740608229, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17559526, Length=530, Percent_Identity=25.0943396226415, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71996755, Length=521, Percent_Identity=25.1439539347409, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI32563687, Length=490, Percent_Identity=21.8367346938776, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560140, Length=532, Percent_Identity=23.8721804511278, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17558820, Length=482, Percent_Identity=21.7842323651452, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71982997, Length=567, Percent_Identity=24.8677248677249, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71983001, Length=567, Percent_Identity=24.8677248677249, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI193209603, Length=475, Percent_Identity=21.8947368421053, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=364, Percent_Identity=24.7252747252747, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17556356, Length=355, Percent_Identity=23.3802816901408, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=535, Percent_Identity=24.2990654205607, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323182, Length=550, Percent_Identity=25.0909090909091, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6319264, Length=528, Percent_Identity=22.3484848484848, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24581924, Length=547, Percent_Identity=25.7769652650823, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI18859661, Length=519, Percent_Identity=22.9287090558767, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI21355181, Length=531, Percent_Identity=23.1638418079096, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19922652, Length=532, Percent_Identity=22.5563909774436, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24667955, Length=554, Percent_Identity=25.4512635379061, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI62472339, Length=535, Percent_Identity=25.4205607476636, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24656500, Length=532, Percent_Identity=22.9323308270677, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21355225, Length=568, Percent_Identity=22.3591549295775, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24667959, Length=382, Percent_Identity=24.869109947644, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356441, Length=498, Percent_Identity=22.8915662650602, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24648260, Length=533, Percent_Identity=21.3883677298311, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356947, Length=507, Percent_Identity=20.7100591715976, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648253, Length=524, Percent_Identity=21.9465648854962, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281365686, Length=469, Percent_Identity=23.0277185501066, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648255, Length=524, Percent_Identity=21.9465648854962, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21358303, Length=499, Percent_Identity=21.0420841683367, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI19921316, Length=239, Percent_Identity=26.7782426778243, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24653035, Length=296, Percent_Identity=21.2837837837838, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 2.7.7.58

Molecular weight: Translated: 59809; Mature: 59809

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTGYTKWPKEFADRYREEGCWLGETFGGVLRERAEKYGDQIAVVSGNTYITYSELDKKV
CCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCEEEEEECCEEEEHHHHHHHH
DRLASGLLNLGIKPEDRVVIQLPNVIEFFEMCFALFRIGALPVFALPSHRSSEISYFCEF
HHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEC
GEASAYVISDKALGFDYRKLAREVQEKVPTLQHVIVVGEEEEFVNISDLYIDPVPLPEVQ
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEHHHEEECCCCCCCCC
PSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLSAESVYMAVLPVAHNYPMSS
CCCEEEEEECCCCCCHHHHCCCCCCCEEEEEEEEHHHHCCCHHHEEEEEEEHCCCCCCCC
PGTFGTFYAGGKVVLATGGSPDETFALIEKEKVTITALVPPLAMIWLDAASLRNNDLSSL
CCCCEEEECCCEEEEEECCCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHCCCCCCCE
EVIQVGGAKFSVEVAKRIRPTFGCTLQQVFGMAEGLVNYTRLDDPEEIIIHTQGRPMSAL
EEEEECCCEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHH
DEVRVVDEYDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNARSFTNDGFYRTGDLVKVNEQ
HHHHHHHCCCCCCCCCCHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCEECCCEEEECCC
GYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
CEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHCCCEEEEEEECC
QAPAVSELKRFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLITVKQ
CCCCHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTGYTKWPKEFADRYREEGCWLGETFGGVLRERAEKYGDQIAVVSGNTYITYSELDKKV
CCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHCCEEEEEECCEEEEHHHHHHHH
DRLASGLLNLGIKPEDRVVIQLPNVIEFFEMCFALFRIGALPVFALPSHRSSEISYFCEF
HHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEC
GEASAYVISDKALGFDYRKLAREVQEKVPTLQHVIVVGEEEEFVNISDLYIDPVPLPEVQ
CCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEHHHEEECCCCCCCCC
PSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLSAESVYMAVLPVAHNYPMSS
CCCEEEEEECCCCCCHHHHCCCCCCCEEEEEEEEHHHHCCCHHHEEEEEEEHCCCCCCCC
PGTFGTFYAGGKVVLATGGSPDETFALIEKEKVTITALVPPLAMIWLDAASLRNNDLSSL
CCCCEEEECCCEEEEEECCCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHCCCCCCCE
EVIQVGGAKFSVEVAKRIRPTFGCTLQQVFGMAEGLVNYTRLDDPEEIIIHTQGRPMSAL
EEEEECCCEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHH
DEVRVVDEYDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNARSFTNDGFYRTGDLVKVNEQ
HHHHHHHCCCCCCCCCCHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCEECCCEEEECCC
GYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
CEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHCCCEEEEEEECC
QAPAVSELKRFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLITVKQ
CCCCHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 2,3-dihydroxybenzoate

Specific reaction: ATP + 2,3-dihydroxybenzoate = diphosphate + (2,3-dihydroxybenzoyl)adenylate

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]