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The map label for this gene is pulA [H]

Identifier: 52142777

GI number: 52142777

Start: 2596146

End: 2598698

Strand: Reverse

Name: pulA [H]

Synonym: BCZK2463

Alternate gene names: 52142777

Gene position: 2598698-2596146 (Counterclockwise)

Preceding gene: 52142776

Following gene: 52142778

Centisome position: 49.02

GC content: 34.59

Gene sequence:

>2553_bases
ATGACAAAAAGATTAATTAACAAATCAGTTTTATTGCTTACTATAATCGTTATGTTATCATCTGTTTACTCTTTTCAAAA
TGTAAAGGCAGTTTCAAATTCGAAAACAACTGAAGTTATCATTCATTACAAAGAGCAGTCTGGAAATACAAAAGACTGGA
ACCTTTGGATATGGGGAGAAAATTCAAATGGAAAATCATATGAATTTACGGGTGAAGATGAATTTGGAAAGTACGCAAAG
ATTAATATAGATGGTGATTATAACCGTTTAGGATTTATCATTCGTACAAGTGAGTGGGAAAAAGATGGTGGGGATCGTTG
GATTGAAAATATAAAAGACGGTCGTGCTGAAGTATGGATTCTTTCGGGTGATGAAAAAGTGTATAATTCTAAGCCGTCAT
CGGATCTCTCAATTCAAAAAGCAACTATTGATAGTTTCCATGAAATTACTGTAACGACGAATGTCCCGTTTCATATTAAG
GAAAAGAAAATTGAAATGGAAGGCATTAAAATTAAGAGTATTAGTCCTTATGATATAAACAGTGGAGATATTACGAATAA
GGTTAAAATAATTACGGACCAGAAAATTGATTTAAAACAAACATACAAAGTTAAAATAGAAAACTTAGCTGATACGAATA
CTGAAATCGGTAAAGTAATTCGTACTGAGGAGTTTGATAAATTATTTTATTACGGTGGCAACGATTTAGGTAATATATAT
ACACCACAACATACAAAATTCCGTGTATGGGCCCCTACTGCGAGTGAAGCGAAGTTAGTTACATATAAAAAATGGAACGA
TAAAATAGGTACTGAAATAAATATGCAACAAGGTGAAAAGGGCACCTGGAAAGCAGAACTTAAAGGAAATCAAAAAGGTC
TCTATTATACGTATAAGGTGAAAATTGGTGATAAGTGGACAGAAGCCGTTGATCCGTATGCACGTGCTGCTTCTGTAAAT
GGAGATAAAGGTGCGGTTGTTGATTTAGAAGAAACAAATCCGAAAAGATGGAACACTAACAAAAAGCCTAAATTAAAAAA
TCCGGAAGATGCTATTATTTATGAATTGCATGTACGCGATCTTTCCATCCAACCTGAAAGTGGGATTAAGCAGAAAGGGA
AATATTTAGGAGTAACAGAAAAAGGAACAAAAGGGCCTGAAGGTGTGAAAACGGGACTTGATCATATGAAAGATCTTGGT
GTTACACACGTTCAGCTTTTACCGATATTTGATTATGCTTCAGTAAATGAAGAGAAGGTAAACGAACCACAATATAACTG
GGGATATGACCCAAAAAACTTTAACGTTCCAGAGGGGTCCTATTCTACAAATCCATATGAGCCAACTGTTCGAATAACTG
AATTAAAACAAATGATTCAAACACTACATGATAATAATCTTCGAGTGGTGATGGATGTAGTATATAACCATATGTATAAC
GCTGCTGAATCTAATTTCCATAAATTGGTTCCGGGTTATTATTATCGTTACAATGAAGATGGAACATTTGCAAATGGAAC
TGGAGTAGGAAATGATACTGCTTCAGAAAGAAAAATGATGAGGAAATTTATGATAGATTCCGTCACATATTGGGCAAAAG
AATACAATTTAGATGGATTCCGTTTTGATTTAATGGGTATTCATGATTATGAAACGATGAATGAAATACGTAAAGCTGTT
AATCAAATTGATCCTTCTATTATACTGCATGGAGAAGGTTGGGATTTAAATACACCCCTTGCAGCTGAGTTGAAAGCAAA
TCAAAAAAATGCGGAGAAAATGAAAGGAATTGCCCATTTTAACGATAATATACGTGATGGATTGAAAGGTAGTGTGTTTG
AAGAGAAAGAAAATGGATTTGTAAATGGAAAGGAAAACATGGAAGACCGTATTAAAAAAGGCATTACAGCAGGCATTGAC
TACGATACAAATTCGTCGACTTATCTAGATCCAGAGCAGGTATTAACTTATGTGGAAGCGCATGATAACCATACATTATG
GGATAAACTTGAGTTGACTAATCCAGGTGACAGTGAAGAAGCGCGAAAACAAATGCATAAATTATCTTCTTCGATTTTAT
TAACATCACAAGGTATTCCGTTTTTACATGCAGGGCAAGAGTTTATGCGGACGAAATACGGTGATCATAATAGTTACAAA
TCTCCAGATTCGATTAATCAGATGGATTGGCTGCGCCGTGCAGCGTTTAATAATGAAGTAGATTATATGAAGGGTTTAAT
AGAATTACGCAAAAAGTACCCAGCTTTTCGAATGACATCTGCAGAGCAAATAAAAACACATGTATCATTTATTGATGCTC
CGAAAAATACTGTAGCTTATACAATAGAGGGGAATAAAAATGAATACTTTACGGTGGCTCATAATGCAAATAGGGAATCT
GTTGAAATCTCACTTCCATCGAAAGGTCCTTGGAAAGTACTAGTAGACGGGAAGCAGGCGGGAAGTAAACCACTTTATGT
TGTCCATGATAATAAAATTAAAGTTCCTGCGCTAAGTTCTTTAGTTTTAAAAACAGAGAAACCGATTAAATAA

Upstream 100 bases:

>100_bases
GTGAAGCAAAAGAGATTTCTAGATTAATTTAAATTGTGTAATGTAGTACAGGCATAATAAATCGTTCGTACTATTTAAAG
AAAGGGTGTATGTGGTGCAA

Downstream 100 bases:

>100_bases
ATGCCAAAAGCGGAGTGAAGAAGGTTGTTTTCACTTCGCTTTTAAAGTTAAAATAGTATAATTTCACATACTGTAATTAT
ATTACAATTCAATAAAAAAA

Product: pullulanase

Products: NA

Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]

Number of amino acids: Translated: 850; Mature: 849

Protein sequence:

>850_residues
MTKRLINKSVLLLTIIVMLSSVYSFQNVKAVSNSKTTEVIIHYKEQSGNTKDWNLWIWGENSNGKSYEFTGEDEFGKYAK
INIDGDYNRLGFIIRTSEWEKDGGDRWIENIKDGRAEVWILSGDEKVYNSKPSSDLSIQKATIDSFHEITVTTNVPFHIK
EKKIEMEGIKIKSISPYDINSGDITNKVKIITDQKIDLKQTYKVKIENLADTNTEIGKVIRTEEFDKLFYYGGNDLGNIY
TPQHTKFRVWAPTASEAKLVTYKKWNDKIGTEINMQQGEKGTWKAELKGNQKGLYYTYKVKIGDKWTEAVDPYARAASVN
GDKGAVVDLEETNPKRWNTNKKPKLKNPEDAIIYELHVRDLSIQPESGIKQKGKYLGVTEKGTKGPEGVKTGLDHMKDLG
VTHVQLLPIFDYASVNEEKVNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMIQTLHDNNLRVVMDVVYNHMYN
AAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERKMMRKFMIDSVTYWAKEYNLDGFRFDLMGIHDYETMNEIRKAV
NQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKGIAHFNDNIRDGLKGSVFEEKENGFVNGKENMEDRIKKGITAGID
YDTNSSTYLDPEQVLTYVEAHDNHTLWDKLELTNPGDSEEARKQMHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNSYK
SPDSINQMDWLRRAAFNNEVDYMKGLIELRKKYPAFRMTSAEQIKTHVSFIDAPKNTVAYTIEGNKNEYFTVAHNANRES
VEISLPSKGPWKVLVDGKQAGSKPLYVVHDNKIKVPALSSLVLKTEKPIK

Sequences:

>Translated_850_residues
MTKRLINKSVLLLTIIVMLSSVYSFQNVKAVSNSKTTEVIIHYKEQSGNTKDWNLWIWGENSNGKSYEFTGEDEFGKYAK
INIDGDYNRLGFIIRTSEWEKDGGDRWIENIKDGRAEVWILSGDEKVYNSKPSSDLSIQKATIDSFHEITVTTNVPFHIK
EKKIEMEGIKIKSISPYDINSGDITNKVKIITDQKIDLKQTYKVKIENLADTNTEIGKVIRTEEFDKLFYYGGNDLGNIY
TPQHTKFRVWAPTASEAKLVTYKKWNDKIGTEINMQQGEKGTWKAELKGNQKGLYYTYKVKIGDKWTEAVDPYARAASVN
GDKGAVVDLEETNPKRWNTNKKPKLKNPEDAIIYELHVRDLSIQPESGIKQKGKYLGVTEKGTKGPEGVKTGLDHMKDLG
VTHVQLLPIFDYASVNEEKVNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMIQTLHDNNLRVVMDVVYNHMYN
AAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERKMMRKFMIDSVTYWAKEYNLDGFRFDLMGIHDYETMNEIRKAV
NQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKGIAHFNDNIRDGLKGSVFEEKENGFVNGKENMEDRIKKGITAGID
YDTNSSTYLDPEQVLTYVEAHDNHTLWDKLELTNPGDSEEARKQMHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNSYK
SPDSINQMDWLRRAAFNNEVDYMKGLIELRKKYPAFRMTSAEQIKTHVSFIDAPKNTVAYTIEGNKNEYFTVAHNANRES
VEISLPSKGPWKVLVDGKQAGSKPLYVVHDNKIKVPALSSLVLKTEKPIK
>Mature_849_residues
TKRLINKSVLLLTIIVMLSSVYSFQNVKAVSNSKTTEVIIHYKEQSGNTKDWNLWIWGENSNGKSYEFTGEDEFGKYAKI
NIDGDYNRLGFIIRTSEWEKDGGDRWIENIKDGRAEVWILSGDEKVYNSKPSSDLSIQKATIDSFHEITVTTNVPFHIKE
KKIEMEGIKIKSISPYDINSGDITNKVKIITDQKIDLKQTYKVKIENLADTNTEIGKVIRTEEFDKLFYYGGNDLGNIYT
PQHTKFRVWAPTASEAKLVTYKKWNDKIGTEINMQQGEKGTWKAELKGNQKGLYYTYKVKIGDKWTEAVDPYARAASVNG
DKGAVVDLEETNPKRWNTNKKPKLKNPEDAIIYELHVRDLSIQPESGIKQKGKYLGVTEKGTKGPEGVKTGLDHMKDLGV
THVQLLPIFDYASVNEEKVNEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMIQTLHDNNLRVVMDVVYNHMYNA
AESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERKMMRKFMIDSVTYWAKEYNLDGFRFDLMGIHDYETMNEIRKAVN
QIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKGIAHFNDNIRDGLKGSVFEEKENGFVNGKENMEDRIKKGITAGIDY
DTNSSTYLDPEQVLTYVEAHDNHTLWDKLELTNPGDSEEARKQMHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNSYKS
PDSINQMDWLRRAAFNNEVDYMKGLIELRKKYPAFRMTSAEQIKTHVSFIDAPKNTVAYTIEGNKNEYFTVAHNANRESV
EISLPSKGPWKVLVDGKQAGSKPLYVVHDNKIKVPALSSLVLKTEKPIK

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=663, Percent_Identity=22.7752639517345, Blast_Score=119, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR005323
- InterPro:   IPR011840 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; PF03714 PUD [H]

EC number: =3.2.1.41 [H]

Molecular weight: Translated: 97018; Mature: 96887

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRLINKSVLLLTIIVMLSSVYSFQNVKAVSNSKTTEVIIHYKEQSGNTKDWNLWIWGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEEEECCCCCCCCEEEEEEEE
NSNGKSYEFTGEDEFGKYAKINIDGDYNRLGFIIRTSEWEKDGGDRWIENIKDGRAEVWI
CCCCCEEEECCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCEEEEE
LSGDEKVYNSKPSSDLSIQKATIDSFHEITVTTNVPFHIKEKKIEMEGIKIKSISPYDIN
ECCCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCEEEECCEEEECCEEEECCCCCCCC
SGDITNKVKIITDQKIDLKQTYKVKIENLADTNTEIGKVIRTEEFDKLFYYGGNDLGNIY
CCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCC
TPQHTKFRVWAPTASEAKLVTYKKWNDKIGTEINMQQGEKGTWKAELKGNQKGLYYTYKV
CCCCCEEEEECCCCCCCEEEEEEECCCCCCCEEECCCCCCCCEEEEECCCCCCEEEEEEE
KIGDKWTEAVDPYARAASVNGDKGAVVDLEETNPKRWNTNKKPKLKNPEDAIIYELHVRD
EECCCHHHHCCHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEE
LSIQPESGIKQKGKYLGVTEKGTKGPEGVKTGLDHMKDLGVTHVQLLPIFDYASVNEEKV
EEECCCCCHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHC
NEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMIQTLHDNNLRVVMDVVYNHMYN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCEEHHHHHHHHHHH
AAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERKMMRKFMIDSVTYWAKEYNLDGF
HHHCCHHHCCCCEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
RFDLMGIHDYETMNEIRKAVNQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKGIAHF
EEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHEECCCCCHHHHHHHHHHC
NDNIRDGLKGSVFEEKENGFVNGKENMEDRIKKGITAGIDYDTNSSTYLDPEQVLTYVEA
CCCHHCCCCCCCEECCCCCCCCCCCCHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHHHC
HDNHTLWDKLELTNPGDSEEARKQMHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNSYK
CCCCEEEEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCEECCHHHHHHHCCCCCCCC
SPDSINQMDWLRRAAFNNEVDYMKGLIELRKKYPAFRMTSAEQIKTHVSFIDAPKNTVAY
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEECCCHHHHHHHHHEEECCCCEEEE
TIEGNKNEYFTVAHNANRESVEISLPSKGPWKVLVDGKQAGSKPLYVVHDNKIKVPALSS
EEECCCCEEEEEEECCCCCEEEEECCCCCCEEEEEECCCCCCCCEEEEECCEECCCCHHH
LVLKTEKPIK
HHCCCCCCCC
>Mature Secondary Structure 
TKRLINKSVLLLTIIVMLSSVYSFQNVKAVSNSKTTEVIIHYKEQSGNTKDWNLWIWGE
CHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEEEECCCCCCCCEEEEEEEE
NSNGKSYEFTGEDEFGKYAKINIDGDYNRLGFIIRTSEWEKDGGDRWIENIKDGRAEVWI
CCCCCEEEECCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCEEEEE
LSGDEKVYNSKPSSDLSIQKATIDSFHEITVTTNVPFHIKEKKIEMEGIKIKSISPYDIN
ECCCCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCEEEECCEEEECCEEEECCCCCCCC
SGDITNKVKIITDQKIDLKQTYKVKIENLADTNTEIGKVIRTEEFDKLFYYGGNDLGNIY
CCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCC
TPQHTKFRVWAPTASEAKLVTYKKWNDKIGTEINMQQGEKGTWKAELKGNQKGLYYTYKV
CCCCCEEEEECCCCCCCEEEEEEECCCCCCCEEECCCCCCCCEEEEECCCCCCEEEEEEE
KIGDKWTEAVDPYARAASVNGDKGAVVDLEETNPKRWNTNKKPKLKNPEDAIIYELHVRD
EECCCHHHHCCHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEEE
LSIQPESGIKQKGKYLGVTEKGTKGPEGVKTGLDHMKDLGVTHVQLLPIFDYASVNEEKV
EEECCCCCHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHC
NEPQYNWGYDPKNFNVPEGSYSTNPYEPTVRITELKQMIQTLHDNNLRVVMDVVYNHMYN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCEEHHHHHHHHHHH
AAESNFHKLVPGYYYRYNEDGTFANGTGVGNDTASERKMMRKFMIDSVTYWAKEYNLDGF
HHHCCHHHCCCCEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCE
RFDLMGIHDYETMNEIRKAVNQIDPSIILHGEGWDLNTPLAAELKANQKNAEKMKGIAHF
EEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHEECCCCCHHHHHHHHHHC
NDNIRDGLKGSVFEEKENGFVNGKENMEDRIKKGITAGIDYDTNSSTYLDPEQVLTYVEA
CCCHHCCCCCCCEECCCCCCCCCCCCHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHHHC
HDNHTLWDKLELTNPGDSEEARKQMHKLSSSILLTSQGIPFLHAGQEFMRTKYGDHNSYK
CCCCEEEEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCEECCHHHHHHHCCCCCCCC
SPDSINQMDWLRRAAFNNEVDYMKGLIELRKKYPAFRMTSAEQIKTHVSFIDAPKNTVAY
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEECCCHHHHHHHHHEEECCCCEEEE
TIEGNKNEYFTVAHNANRESVEISLPSKGPWKVLVDGKQAGSKPLYVVHDNKIKVPALSS
EEECCCCEEEEEEECCCCCEEEEECCCCCCEEEEEECCCCCCCCEEEEECCEECCCCHHH
LVLKTEKPIK
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9453151; 10360571 [H]