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The map label for this gene is alsT [H]

Identifier: 52142385

GI number: 52142385

Start: 2977200

End: 2978624

Strand: Reverse

Name: alsT [H]

Synonym: BCZK2858

Alternate gene names: 52142385

Gene position: 2978624-2977200 (Counterclockwise)

Preceding gene: 52142382

Following gene: 52142384

Centisome position: 56.19

GC content: 34.81

Gene sequence:

>1425_bases
ATGGAAAGGTTTTTTGTAGATTTAGTCGGATCAACGAATGATTTTTTATGGTCTAAATTGTTAATTATTATGCTTGTTAT
TTGCGGAATTTATTTTACATTTAAATTGAATTTTGTACAGTTTCGAATGTTAAAAGAGATGGTCCGTGTATTAATGGAGG
GGAAAAGTGGTTCTAAAGATCAAATCTCTCCTTTTCAAGCGTTTTGTATTGGTATGGCAGCCCGCGTTGGAACAGGGAAT
ATCACAGGAATTGCGATTGCAATTGCATTAGGTGGTCCTGGAGCTGTATTTTGGATGTGGATTATTTCTATTATTAGCTC
GGCCTCCAGTTTTGTCGAAAGCACATTAGCACAGATATATAAAGTAAAAGATAAAACTGGTTTCCGCGGTGGTCCATCCT
ATTATATGGAAAAAGGGTTGAATAAACGTTGGATGGGAGTATTATTCTCTATTTTAATCACAATTACTTTTGGTCTTGTA
TTCAATTCAGTACAATCAAATACAGTTACAATCGCTTTTCAAAACTCATTTGGTACAGATCGTCTAACGGTTGGGATAAT
CATGGCCATTGCTTTTGCTGCAATTATCTTTGGTGGTGTACAACGTATTGCAAAAATGGCTGAATACAAGGTGGTATTTT
TAGCGGTATTATACATTGGAGTTGCATTATTTGTTGTAGTTACCAACATATCAAAAATGCCGGAAGTTCTTTCTCTTATT
GTTCAAAACGCATTTGGTTTTAAACAAATAGCAGGTGGTTCAATTGGTGCAGCACTTATGAATGGAGTTAAACGCGGTTT
ATTCTCAAATGAAGCTGGTATGGGAAGTGCACCAAACGTTGCCGCAACAGCAACAACAAGCCATCCGGTGAAACAAGGAC
TGATTCAAGCATTTGGTGTATTAACGGATACATTAATCATTTGTACTAGCACTGCCTTTATTATTTTGCTGTCTGATGCA
TATAAACAACCGGGATTAAATGGTATTGCACTGACACAAGCAGCATTAAGTGAACACATCGGTCCTTGGGCATCGGGTTG
TCTTGCTATCTTTGTTTTTTTATTTGGATTTGGTGCGTTAATTGGCAACTATTATTATGGTGAAACAAATATTCGCTTCT
TACATACAAGTAAAGTGTGGTTAATGATATACAGAATAAGTGTATTGGCTATGATTGTATTTGGTTCTGTTGCAAAGATT
CAAATTGTATGGGATTTAGCGGACTTATTTATGGGCTTTATGGTTATTATTAATTTAATTGCTATTACCTTACTTTCAAA
AGTAGCATTTGCGGCATTACAAGATTATATAAACCAGAAAAAAGCTGGTAAAGATCCTATTTTTTATAAAGAAAACATTA
AAGGGCTTGAAAATATTGAATGTTGGGATAGTTATGAGAAAACTTCAAAAAAGAAAAGTGTGTAA

Upstream 100 bases:

>100_bases
ATCCTTGTTGTATTTGAATATGAATGAGGTATTTATGTCAGGGAATATAGGTGATTTTATATAGATTATGATAGATAAAT
TTTTGGTATGGGGTGATAAT

Downstream 100 bases:

>100_bases
TAAGAAGGATATTCAGACTGCAATATGTCTGAATATTTTTCTTGGTTCTAGTGCAAATATTATTGATTATAGTAAAGAAA
AAATCATTCTATATAAAGTT

Product: sodium/alanine symporter family protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MERFFVDLVGSTNDFLWSKLLIIMLVICGIYFTFKLNFVQFRMLKEMVRVLMEGKSGSKDQISPFQAFCIGMAARVGTGN
ITGIAIAIALGGPGAVFWMWIISIISSASSFVESTLAQIYKVKDKTGFRGGPSYYMEKGLNKRWMGVLFSILITITFGLV
FNSVQSNTVTIAFQNSFGTDRLTVGIIMAIAFAAIIFGGVQRIAKMAEYKVVFLAVLYIGVALFVVVTNISKMPEVLSLI
VQNAFGFKQIAGGSIGAALMNGVKRGLFSNEAGMGSAPNVAATATTSHPVKQGLIQAFGVLTDTLIICTSTAFIILLSDA
YKQPGLNGIALTQAALSEHIGPWASGCLAIFVFLFGFGALIGNYYYGETNIRFLHTSKVWLMIYRISVLAMIVFGSVAKI
QIVWDLADLFMGFMVIINLIAITLLSKVAFAALQDYINQKKAGKDPIFYKENIKGLENIECWDSYEKTSKKKSV

Sequences:

>Translated_474_residues
MERFFVDLVGSTNDFLWSKLLIIMLVICGIYFTFKLNFVQFRMLKEMVRVLMEGKSGSKDQISPFQAFCIGMAARVGTGN
ITGIAIAIALGGPGAVFWMWIISIISSASSFVESTLAQIYKVKDKTGFRGGPSYYMEKGLNKRWMGVLFSILITITFGLV
FNSVQSNTVTIAFQNSFGTDRLTVGIIMAIAFAAIIFGGVQRIAKMAEYKVVFLAVLYIGVALFVVVTNISKMPEVLSLI
VQNAFGFKQIAGGSIGAALMNGVKRGLFSNEAGMGSAPNVAATATTSHPVKQGLIQAFGVLTDTLIICTSTAFIILLSDA
YKQPGLNGIALTQAALSEHIGPWASGCLAIFVFLFGFGALIGNYYYGETNIRFLHTSKVWLMIYRISVLAMIVFGSVAKI
QIVWDLADLFMGFMVIINLIAITLLSKVAFAALQDYINQKKAGKDPIFYKENIKGLENIECWDSYEKTSKKKSV
>Mature_474_residues
MERFFVDLVGSTNDFLWSKLLIIMLVICGIYFTFKLNFVQFRMLKEMVRVLMEGKSGSKDQISPFQAFCIGMAARVGTGN
ITGIAIAIALGGPGAVFWMWIISIISSASSFVESTLAQIYKVKDKTGFRGGPSYYMEKGLNKRWMGVLFSILITITFGLV
FNSVQSNTVTIAFQNSFGTDRLTVGIIMAIAFAAIIFGGVQRIAKMAEYKVVFLAVLYIGVALFVVVTNISKMPEVLSLI
VQNAFGFKQIAGGSIGAALMNGVKRGLFSNEAGMGSAPNVAATATTSHPVKQGLIQAFGVLTDTLIICTSTAFIILLSDA
YKQPGLNGIALTQAALSEHIGPWASGCLAIFVFLFGFGALIGNYYYGETNIRFLHTSKVWLMIYRISVLAMIVFGSVAKI
QIVWDLADLFMGFMVIINLIAITLLSKVAFAALQDYINQKKAGKDPIFYKENIKGLENIECWDSYEKTSKKKSV

Specific function: Probably functions as a sodium/glutamine symporter for glutamine uptake [H]

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=476, Percent_Identity=38.0252100840336, Blast_Score=330, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 51765; Mature: 51765

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERFFVDLVGSTNDFLWSKLLIIMLVICGIYFTFKLNFVQFRMLKEMVRVLMEGKSGSKD
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QISPFQAFCIGMAARVGTGNITGIAIAIALGGPGAVFWMWIISIISSASSFVESTLAQIY
CCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVKDKTGFRGGPSYYMEKGLNKRWMGVLFSILITITFGLVFNSVQSNTVTIAFQNSFGTD
HHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCH
RLTVGIIMAIAFAAIIFGGVQRIAKMAEYKVVFLAVLYIGVALFVVVTNISKMPEVLSLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQNAFGFKQIAGGSIGAALMNGVKRGLFSNEAGMGSAPNVAATATTSHPVKQGLIQAFGV
HHHCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHH
LTDTLIICTSTAFIILLSDAYKQPGLNGIALTQAALSEHIGPWASGCLAIFVFLFGFGAL
HHHHHHHHHHHHHHHEEEHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
IGNYYYGETNIRFLHTSKVWLMIYRISVLAMIVFGSVAKIQIVWDLADLFMGFMVIINLI
HCCEEECCCCEEEEECCHHHHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHHHHHH
AITLLSKVAFAALQDYINQKKAGKDPIFYKENIKGLENIECWDSYEKTSKKKSV
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MERFFVDLVGSTNDFLWSKLLIIMLVICGIYFTFKLNFVQFRMLKEMVRVLMEGKSGSKD
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QISPFQAFCIGMAARVGTGNITGIAIAIALGGPGAVFWMWIISIISSASSFVESTLAQIY
CCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVKDKTGFRGGPSYYMEKGLNKRWMGVLFSILITITFGLVFNSVQSNTVTIAFQNSFGTD
HHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCH
RLTVGIIMAIAFAAIIFGGVQRIAKMAEYKVVFLAVLYIGVALFVVVTNISKMPEVLSLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQNAFGFKQIAGGSIGAALMNGVKRGLFSNEAGMGSAPNVAATATTSHPVKQGLIQAFGV
HHHCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHH
LTDTLIICTSTAFIILLSDAYKQPGLNGIALTQAALSEHIGPWASGCLAIFVFLFGFGAL
HHHHHHHHHHHHHHHEEEHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
IGNYYYGETNIRFLHTSKVWLMIYRISVLAMIVFGSVAKIQIVWDLADLFMGFMVIINLI
HCCEEECCCCEEEEECCHHHHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHHHHHH
AITLLSKVAFAALQDYINQKKAGKDPIFYKENIKGLENIECWDSYEKTSKKKSV
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]