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The map label for this gene is rfbF [H]

Identifier: 52142172

GI number: 52142172

Start: 3196587

End: 3197354

Strand: Direct

Name: rfbF [H]

Synonym: BCZK3069

Alternate gene names: 52142172

Gene position: 3196587-3197354 (Clockwise)

Preceding gene: 52142174

Following gene: 52142173

Centisome position: 60.3

GC content: 36.07

Gene sequence:

>768_bases
ATGAAAGCTGTTATTCTTGCTGGTGGATATGGGACAAGGATTGGGGAAGAAACACATTTAAAACCGAAACCTATGATTGA
AATTGGAACAAAGCCTATTTTATGGCACATTATGAGTTTGTATAGCCATTACGGCATTACAGAGTTTATTATTTGCTTAG
GGTATAAAGGATATGCAATTAAAGAATTCTTTCTAAATTACAACTTACACATGTCAGATTTCACGATACATTTAAAGGAT
AATACAATTACTAGCCATTCTCATCGTGTAGAACCGTGGAAAGTTACACTAATCGATACTGGTGTAAATACAGAAACGGG
AGGACGAGTGAAAAAGATTCAAAATTTTGTTGGAGACGAACCTTTCTGCCTCACTTATGGCGATGGATTAAGTAACGTAA
ATATAAAAGAACTTATTGCATTTCATAAAAAACATGGAAAAATGGCAACTGTTACCGCCGTACAACCTCCTGGTAGATTC
GGTTCTCTCATATTAGATAAACAATCTGTAACATCCTTTCAAGAGAAGCCACTTGGGGATGGCGGATGGGTTAACGGTGG
ATTTTTCGTTTTAAATCCTGAAGTTTTCAATTACATTAGTGGAGATAAGTCTGTTTTTGAAACAGACACTTTAGTTCAGT
TAGTAAACAAAAACGAACTATCAGCATATCAACATGCTGGTTTTTGGCATCCGATGGATACATTGCGCGATAAAAATAAA
TTAGTCGAGCTATGGGAAAGTAATAACGCTCCGTGGAAGGTTTGGTAA

Upstream 100 bases:

>100_bases
ATCATGCTTCACATATTTTTATGCACTTAGTCAAAACGAAAACCATTTGCATACAATATGAATACTGTATATATTATATT
TTTTATTTTAGGAGGAAAAA

Downstream 100 bases:

>100_bases
TATGGCTTCATCATCTTTTTGGAATAAAAAAAAAGTATTTATTACGGGACATACTGGATTTAAAGGTTCCTGGCTCACCC
TTTTCTTAACTTCTTTAGGT

Product: glucose-1-phosphate cytidylyltransferase

Products: NA

Alternate protein names: CDP-glucose pyrophosphorylase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKAVILAGGYGTRIGEETHLKPKPMIEIGTKPILWHIMSLYSHYGITEFIICLGYKGYAIKEFFLNYNLHMSDFTIHLKD
NTITSHSHRVEPWKVTLIDTGVNTETGGRVKKIQNFVGDEPFCLTYGDGLSNVNIKELIAFHKKHGKMATVTAVQPPGRF
GSLILDKQSVTSFQEKPLGDGGWVNGGFFVLNPEVFNYISGDKSVFETDTLVQLVNKNELSAYQHAGFWHPMDTLRDKNK
LVELWESNNAPWKVW

Sequences:

>Translated_255_residues
MKAVILAGGYGTRIGEETHLKPKPMIEIGTKPILWHIMSLYSHYGITEFIICLGYKGYAIKEFFLNYNLHMSDFTIHLKD
NTITSHSHRVEPWKVTLIDTGVNTETGGRVKKIQNFVGDEPFCLTYGDGLSNVNIKELIAFHKKHGKMATVTAVQPPGRF
GSLILDKQSVTSFQEKPLGDGGWVNGGFFVLNPEVFNYISGDKSVFETDTLVQLVNKNELSAYQHAGFWHPMDTLRDKNK
LVELWESNNAPWKVW
>Mature_255_residues
MKAVILAGGYGTRIGEETHLKPKPMIEIGTKPILWHIMSLYSHYGITEFIICLGYKGYAIKEFFLNYNLHMSDFTIHLKD
NTITSHSHRVEPWKVTLIDTGVNTETGGRVKKIQNFVGDEPFCLTYGDGLSNVNIKELIAFHKKHGKMATVTAVQPPGRF
GSLILDKQSVTSFQEKPLGDGGWVNGGFFVLNPEVFNYISGDKSVFETDTLVQLVNKNELSAYQHAGFWHPMDTLRDKNK
LVELWESNNAPWKVW

Specific function: Involved in the biosynthesis of the tyvelose, a 3,6- dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate. This enzyme can utilize either CTP or UTP as the nucle

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate cytidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=248, Percent_Identity=25.4032258064516, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI11761619, Length=248, Percent_Identity=25.4032258064516, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI133931050, Length=246, Percent_Identity=26.0162601626016, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320148, Length=249, Percent_Identity=28.5140562248996, Blast_Score=103, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21355443, Length=240, Percent_Identity=27.0833333333333, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24644084, Length=240, Percent_Identity=27.0833333333333, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013446
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.33 [H]

Molecular weight: Translated: 28848; Mature: 28848

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVILAGGYGTRIGEETHLKPKPMIEIGTKPILWHIMSLYSHYGITEFIICLGYKGYAI
CCEEEEECCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCHHHHHHHHCCCCCEE
KEFFLNYNLHMSDFTIHLKDNTITSHSHRVEPWKVTLIDTGVNTETGGRVKKIQNFVGDE
EEEEEECEEEEEEEEEEEECCEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCC
PFCLTYGDGLSNVNIKELIAFHKKHGKMATVTAVQPPGRFGSLILDKQSVTSFQEKPLGD
CEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECHHHHHHHHHCCCCC
GGWVNGGFFVLNPEVFNYISGDKSVFETDTLVQLVNKNELSAYQHAGFWHPMDTLRDKNK
CCEECCCEEEECHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHH
LVELWESNNAPWKVW
HHHHHHCCCCCEEEC
>Mature Secondary Structure
MKAVILAGGYGTRIGEETHLKPKPMIEIGTKPILWHIMSLYSHYGITEFIICLGYKGYAI
CCEEEEECCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCHHHHHHHHCCCCCEE
KEFFLNYNLHMSDFTIHLKDNTITSHSHRVEPWKVTLIDTGVNTETGGRVKKIQNFVGDE
EEEEEECEEEEEEEEEEEECCEEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCC
PFCLTYGDGLSNVNIKELIAFHKKHGKMATVTAVQPPGRFGSLILDKQSVTSFQEKPLGD
CEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECHHHHHHHHHCCCCC
GGWVNGGFFVLNPEVFNYISGDKSVFETDTLVQLVNKNELSAYQHAGFWHPMDTLRDKNK
CCEECCCEEEECHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHH
LVELWESNNAPWKVW
HHHHHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]