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The map label for this gene is cwl [H]

Identifier: 52141867

GI number: 52141867

Start: 3514980

End: 3515564

Strand: Reverse

Name: cwl [H]

Synonym: BCZK3380

Alternate gene names: 52141867

Gene position: 3515564-3514980 (Counterclockwise)

Preceding gene: 52141864

Following gene: 52141866

Centisome position: 66.32

GC content: 37.78

Gene sequence:

>585_bases
TTGGAGTGGGAACCATTCTTACAAGGAACAAAAGTGTATTATGATGCGAACCATAAAGATGATATAACGGGTGGTTGGTA
CGAAGCAGACATTCGTGAATTAGATAAGCGCGGCATTATGGTTGGAGATGGGAAAGGTTCTTATTGGCCAGAGCGTCTTG
TGACACGTGGTGAATTTGCGAACTTCATTTCAAGATCATTGCAGTTACCTGAAGGATCATCGAATTTTAGTGACTTAAAT
GCAGCACATCCATCGCTAATAGATGGTATTAATCGTGCAGCAAGTGCCGGGATAATAAGTGGTAGAGGAAATGGGATATT
TGCTCCAAATGATACGATTAAACGAGATGAAGTTGTTATCATGATTGACCGTGCACTGCAATATAAAAAAATTAAAGGTA
ACTTAGTTCCACTGCCATTCTCGGATCAAGATTTAGCATATGATAAACAAGCTGTACAACGTGTTTATGGATTAGGCATT
GTAAAAGGAAATGAAAATAATGAATTTATGCCGAAAGGTTCAGCAACGCGTGGAGAATCAGCAGCATTTATTAATAGAAT
GTTAGAAGTAATAGAAAGTAATTAA

Upstream 100 bases:

>100_bases
ATGGTGATTATAATAAAGCCGTTGAAAATGCTCGTAAACTAGCAGCTTATTTAATGGGGGAATTAAATATTCCTTTAGAG
AATGTAAAAAAACATCAATT

Downstream 100 bases:

>100_bases
TAAAAAAGCATTCGCTTTTGCGAGTGCTTTTTTATTTTTCAATATGTCACGGAAGGGAGTGGAGGTTTTTGAGTGGAATA
AGGAATGAAAGTGGAGGTGA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Alpha-amylase/pullulanase; Pullulanase type II; Alpha-amylase; 1,4-alpha-D-glucan glucanohydrolase; Pullulanase; 1,4-alpha-D-glucan glucanohydrolase; Alpha-dextrin endo-1,6-alpha-glucosidase [H]

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MEWEPFLQGTKVYYDANHKDDITGGWYEADIRELDKRGIMVGDGKGSYWPERLVTRGEFANFISRSLQLPEGSSNFSDLN
AAHPSLIDGINRAASAGIISGRGNGIFAPNDTIKRDEVVIMIDRALQYKKIKGNLVPLPFSDQDLAYDKQAVQRVYGLGI
VKGNENNEFMPKGSATRGESAAFINRMLEVIESN

Sequences:

>Translated_194_residues
MEWEPFLQGTKVYYDANHKDDITGGWYEADIRELDKRGIMVGDGKGSYWPERLVTRGEFANFISRSLQLPEGSSNFSDLN
AAHPSLIDGINRAASAGIISGRGNGIFAPNDTIKRDEVVIMIDRALQYKKIKGNLVPLPFSDQDLAYDKQAVQRVYGLGI
VKGNENNEFMPKGSATRGESAAFINRMLEVIESN
>Mature_194_residues
MEWEPFLQGTKVYYDANHKDDITGGWYEADIRELDKRGIMVGDGKGSYWPERLVTRGEFANFISRSLQLPEGSSNFSDLN
AAHPSLIDGINRAASAGIISGRGNGIFAPNDTIKRDEVVIMIDRALQYKKIKGNLVPLPFSDQDLAYDKQAVQRVYGLGI
VKGNENNEFMPKGSATRGESAAFINRMLEVIESN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted, cell wall. Note=It C-terminus may serve as an S-layer anchor [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 3 SLH (S-layer homology) domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013784
- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR002044
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR001119 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N; PF00395 SLH [H]

EC number: =3.2.1.1; =3.2.1.41 [H]

Molecular weight: Translated: 21547; Mature: 21547

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWEPFLQGTKVYYDANHKDDITGGWYEADIRELDKRGIMVGDGKGSYWPERLVTRGEFA
CCCCCCCCCCEEEECCCCCCCCCCCEEHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHH
NFISRSLQLPEGSSNFSDLNAAHPSLIDGINRAASAGIISGRGNGIFAPNDTIKRDEVVI
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEECCCCEEECCCCCCCCCCEEE
MIDRALQYKKIKGNLVPLPFSDQDLAYDKQAVQRVYGLGIVKGNENNEFMPKGSATRGES
EECCCHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCHH
AAFINRMLEVIESN
HHHHHHHHHHHHCC
>Mature Secondary Structure
MEWEPFLQGTKVYYDANHKDDITGGWYEADIRELDKRGIMVGDGKGSYWPERLVTRGEFA
CCCCCCCCCCEEEECCCCCCCCCCCEEHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHH
NFISRSLQLPEGSSNFSDLNAAHPSLIDGINRAASAGIISGRGNGIFAPNDTIKRDEVVI
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEECCCCEEECCCCCCCCCCEEE
MIDRALQYKKIKGNLVPLPFSDQDLAYDKQAVQRVYGLGIVKGNENNEFMPKGSATRGES
EECCCHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCHH
AAFINRMLEVIESN
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8195085 [H]