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The map label for this gene is cwl [H]

Identifier: 52141864

GI number: 52141864

Start: 3515498

End: 3516667

Strand: Reverse

Name: cwl [H]

Synonym: BCZK3381

Alternate gene names: 52141864

Gene position: 3516667-3515498 (Counterclockwise)

Preceding gene: 52141862

Following gene: 52141867

Centisome position: 66.34

GC content: 35.73

Gene sequence:

>1170_bases
ATGAAAAAAGGATTTTATAATGTTTTAGCTGCATCAATTGTATTTAGTATGGCAACGATTCCAAATTATTCCTATGCTAA
CGAACTTGACAAAACGGTTACAGTTTCACCTGATGAACAAGCTTTAAAAAGTATTGAAAACCATATGAAAGATGAAGATG
GTAGAGGAGAAGATAAAGGAGTAAGAAATGAAGTGCAAGGTGAATTTCTTGTACATATAGTGAAAGAGGTACCTTTATAC
GATTCTAGTAATTTTCAAAAAGAGACAGGGGTTCGTATTTCGAATCAAGTCGTAAAAGCTGAGAAGAGAAAAGGAAACGC
TTACTATGTTCAAACTTCATCGGGAACTGGTTGGATACAAAATAGCGATGGGAATGTAGAAGTTAAAGAAATTCACCCGT
TATTAAGTGAGAAGTTAATTGTAAATGAAGAAACCTCTACATACTCTGAACCATTTGCTTCATACAAAGAAGAAAATGTA
CTGGAGCCACAAACTATTCAAGCAATTGGACAGGCTGGAGAATGGTTCCAAGTAAAAATAAATAATGAGATGAAATGGAT
TCATTCACCTTCAGCAAAATTTGAAGGAACAAAAGCTTCGCTTATACAAGGTGCAGCACCAATACGTACAAAATATGCAG
CAGCTATGTATGCTGCACCAATAGAAGAGAAGACAACGGATATTTATGGTGTACCTTTAAAAGAAATGATTGTACCGAAA
GGAAATGAAAATATTCGTCCGGGTTATGCGATGAACCCGAAGTATATTACGATTCATGAGACAGATAATTACAGTGTGGG
GGCAAATGCTAGAAACCATGCGATATATTTATATAACCAAGCAACGGGAACGGAAGATCGTTCAGCGTCGTGGCATTTTA
CAGTAGATGATAAAGAGATTTATCAACACCTGCCATTAAACGAAAATGCTTGGCATGCAGGAGATGGGGCAGAAGGAACT
GGAAATCGAGAATCAATTGCGATTGAAATTGCTGTAAACGAAGATGGTGATTATAATAAAGCCGTTGAAAATGCTCGTAA
ACTAGCAGCTTATTTAATGGGGGAATTAAATATTCCTTTAGAGAATGTAAAAAAACATCAATTTTGGAGTGGGAACCATT
CTTACAAGGAACAAAAGTGTATTATGATGCGAACCATAAAGATGATATAA

Upstream 100 bases:

>100_bases
TAAATGGTTATGTTGTTTAATAGTTTAGTAGATAACCTATATTTTGGCTGAGGAGGGGAAATTTAACACGAACCTAATTA
TATGCGTGTTAAGTAGTATG

Downstream 100 bases:

>100_bases
CGGGTGGTTGGTACGAAGCAGACATTCGTGAATTAGATAAGCGCGGCATTATGGTTGGAGATGGGAAAGGTTCTTATTGG
CCAGAGCGTCTTGTGACACG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MKKGFYNVLAASIVFSMATIPNYSYANELDKTVTVSPDEQALKSIENHMKDEDGRGEDKGVRNEVQGEFLVHIVKEVPLY
DSSNFQKETGVRISNQVVKAEKRKGNAYYVQTSSGTGWIQNSDGNVEVKEIHPLLSEKLIVNEETSTYSEPFASYKEENV
LEPQTIQAIGQAGEWFQVKINNEMKWIHSPSAKFEGTKASLIQGAAPIRTKYAAAMYAAPIEEKTTDIYGVPLKEMIVPK
GNENIRPGYAMNPKYITIHETDNYSVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEIYQHLPLNENAWHAGDGAEGT
GNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPLENVKKHQFWSGNHSYKEQKCIMMRTIKMI

Sequences:

>Translated_389_residues
MKKGFYNVLAASIVFSMATIPNYSYANELDKTVTVSPDEQALKSIENHMKDEDGRGEDKGVRNEVQGEFLVHIVKEVPLY
DSSNFQKETGVRISNQVVKAEKRKGNAYYVQTSSGTGWIQNSDGNVEVKEIHPLLSEKLIVNEETSTYSEPFASYKEENV
LEPQTIQAIGQAGEWFQVKINNEMKWIHSPSAKFEGTKASLIQGAAPIRTKYAAAMYAAPIEEKTTDIYGVPLKEMIVPK
GNENIRPGYAMNPKYITIHETDNYSVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEIYQHLPLNENAWHAGDGAEGT
GNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPLENVKKHQFWSGNHSYKEQKCIMMRTIKMI
>Mature_389_residues
MKKGFYNVLAASIVFSMATIPNYSYANELDKTVTVSPDEQALKSIENHMKDEDGRGEDKGVRNEVQGEFLVHIVKEVPLY
DSSNFQKETGVRISNQVVKAEKRKGNAYYVQTSSGTGWIQNSDGNVEVKEIHPLLSEKLIVNEETSTYSEPFASYKEENV
LEPQTIQAIGQAGEWFQVKINNEMKWIHSPSAKFEGTKASLIQGAAPIRTKYAAAMYAAPIEEKTTDIYGVPLKEMIVPK
GNENIRPGYAMNPKYITIHETDNYSVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEIYQHLPLNENAWHAGDGAEGT
GNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPLENVKKHQFWSGNHSYKEQKCIMMRTIKMI

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 43713; Mature: 43713

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGFYNVLAASIVFSMATIPNYSYANELDKTVTVSPDEQALKSIENHMKDEDGRGEDKG
CCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCC
VRNEVQGEFLVHIVKEVPLYDSSNFQKETGVRISNQVVKAEKRKGNAYYVQTSSGTGWIQ
CHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEE
NSDGNVEVKEIHPLLSEKLIVNEETSTYSEPFASYKEENVLEPQTIQAIGQAGEWFQVKI
CCCCCEEHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEE
NNEMKWIHSPSAKFEGTKASLIQGAAPIRTKYAAAMYAAPIEEKTTDIYGVPLKEMIVPK
CCCEEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEECCCHHHHCCCC
GNENIRPGYAMNPKYITIHETDNYSVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEI
CCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCEEEEEECCCCCCCCCCEEEEEECHHHH
YQHLPLNENAWHAGDGAEGTGNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPL
HHHCCCCCCCEECCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCH
ENVKKHQFWSGNHSYKEQKCIMMRTIKMI
HHHHHHCEECCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKGFYNVLAASIVFSMATIPNYSYANELDKTVTVSPDEQALKSIENHMKDEDGRGEDKG
CCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCC
VRNEVQGEFLVHIVKEVPLYDSSNFQKETGVRISNQVVKAEKRKGNAYYVQTSSGTGWIQ
CHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEE
NSDGNVEVKEIHPLLSEKLIVNEETSTYSEPFASYKEENVLEPQTIQAIGQAGEWFQVKI
CCCCCEEHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEE
NNEMKWIHSPSAKFEGTKASLIQGAAPIRTKYAAAMYAAPIEEKTTDIYGVPLKEMIVPK
CCCEEEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEECCCHHHHCCCC
GNENIRPGYAMNPKYITIHETDNYSVGANARNHAIYLYNQATGTEDRSASWHFTVDDKEI
CCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCEEEEEECCCCCCCCCCEEEEEECHHHH
YQHLPLNENAWHAGDGAEGTGNRESIAIEIAVNEDGDYNKAVENARKLAAYLMGELNIPL
HHHCCCCCCCEECCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCH
ENVKKHQFWSGNHSYKEQKCIMMRTIKMI
HHHHHHCEECCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]