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The map label for this gene is argF [H]

Identifier: 52141366

GI number: 52141366

Start: 4009157

End: 4010107

Strand: Reverse

Name: argF [H]

Synonym: BCZK3883

Alternate gene names: 52141366

Gene position: 4010107-4009157 (Counterclockwise)

Preceding gene: 52141365

Following gene: 52141368

Centisome position: 75.65

GC content: 36.59

Gene sequence:

>951_bases
ATGTCAACTGTACAAGTACCGAAATTAAATACGAAAGATCTTTTAACATTAGAAGAACTAACGCAAGAAGAAATTATTTC
TTTAATTGAGTTCGCTATATATTTAAAAAATAATAAGCAAGAGCCTTTACTACAAGGAAAAATATTAGGCCTTATCTTTG
ATAAGCATTCGACTCGTACTCGTGTATCTTTTGAAGCAGGAATGGTACAGCTCGGGGGACATGGTATGTTTTTAAGTGGG
AAAGAGATGCAAATGGGCAGAGGAGAAACTGTTTCAGATACTGCGAAAGTATTATCGCAGTATATTGACGGGATCATGAT
ACGTACATTCTCACACGCGGATGTAGAAGAGCTTGCAAAAGAATCGAGCATTCCTGTTATTAACGGTTTAACTGACGATC
ATCATCCTTGTCAAGCATTGGCAGACTTAATGACAATATATGAAGAAGTAAATACATTTAAAGGAATAAAATTGGCTTAC
GTAGGTGATGGAAATAATGTATGTCATTCATTGTTGTTAGCGAGTGCAAAAGTCGGAATGCATATGACTGTTGCAACGCC
TATAGGGTATGAACCGAATGAAGAGATTGTAAAAAAAGCATTAGCGATTGCCAAGGAAACAGGAGCTGAAATTGAAATTT
TGCATAACCCTGAATTAGCGGTGAATGAAGCAGATTTCATTTATACTGACGTTTGGATGAGCATGGGGCAAGAGGGCGAA
GAAGAGAAATATACTTTATTTCAACCATATCAAATTAATAAAGAACTTGTTAAGCATGCGAAGCAAACATATCGTTTCTT
ACACTGTTTACCTGCCCATCGTGAAGAAGAGGTAACAGGAGAAATTATAGATGGACCGCAGTCTATCGTCTTTGAACAAG
CTGGTAATCGATTGCATGCTCAAAAAGCGTTATTAGTGAGTTTATTTAAAAATGTAGAAGAGCCTTCCTAA

Upstream 100 bases:

>100_bases
GCCACTCATTGTAACGAATGAAGAGTTAGAACAGGCAGTATATATGATAAAAAAAGTAGTTTGTACAAAAAACGTATCAA
TTATATAAGGGGGAAGTTTC

Downstream 100 bases:

>100_bases
AGGATGTACACCCCAGTTGCTAGATACGAATAACAACTGGGGTGTATTTTTTATATTAAAATGTCATATGTATATTTAGT
TAACCATTTCGATATCATCT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSTVQVPKLNTKDLLTLEELTQEEIISLIEFAIYLKNNKQEPLLQGKILGLIFDKHSTRTRVSFEAGMVQLGGHGMFLSG
KEMQMGRGETVSDTAKVLSQYIDGIMIRTFSHADVEELAKESSIPVINGLTDDHHPCQALADLMTIYEEVNTFKGIKLAY
VGDGNNVCHSLLLASAKVGMHMTVATPIGYEPNEEIVKKALAIAKETGAEIEILHNPELAVNEADFIYTDVWMSMGQEGE
EEKYTLFQPYQINKELVKHAKQTYRFLHCLPAHREEEVTGEIIDGPQSIVFEQAGNRLHAQKALLVSLFKNVEEPS

Sequences:

>Translated_316_residues
MSTVQVPKLNTKDLLTLEELTQEEIISLIEFAIYLKNNKQEPLLQGKILGLIFDKHSTRTRVSFEAGMVQLGGHGMFLSG
KEMQMGRGETVSDTAKVLSQYIDGIMIRTFSHADVEELAKESSIPVINGLTDDHHPCQALADLMTIYEEVNTFKGIKLAY
VGDGNNVCHSLLLASAKVGMHMTVATPIGYEPNEEIVKKALAIAKETGAEIEILHNPELAVNEADFIYTDVWMSMGQEGE
EEKYTLFQPYQINKELVKHAKQTYRFLHCLPAHREEEVTGEIIDGPQSIVFEQAGNRLHAQKALLVSLFKNVEEPS
>Mature_315_residues
STVQVPKLNTKDLLTLEELTQEEIISLIEFAIYLKNNKQEPLLQGKILGLIFDKHSTRTRVSFEAGMVQLGGHGMFLSGK
EMQMGRGETVSDTAKVLSQYIDGIMIRTFSHADVEELAKESSIPVINGLTDDHHPCQALADLMTIYEEVNTFKGIKLAYV
GDGNNVCHSLLLASAKVGMHMTVATPIGYEPNEEIVKKALAIAKETGAEIEILHNPELAVNEADFIYTDVWMSMGQEGEE
EKYTLFQPYQINKELVKHAKQTYRFLHCLPAHREEEVTGEIIDGPQSIVFEQAGNRLHAQKALLVSLFKNVEEPS

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=310, Percent_Identity=44.1935483870968, Blast_Score=271, Evalue=6e-73,
Organism=Homo sapiens, GI18105007, Length=304, Percent_Identity=26.9736842105263, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1790703, Length=324, Percent_Identity=37.6543209876543, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1786469, Length=327, Percent_Identity=37.3088685015291, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=31.0679611650485, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI48994908, Length=358, Percent_Identity=25.9776536312849, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI193204318, Length=323, Percent_Identity=27.2445820433437, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322373, Length=320, Percent_Identity=38.4375, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6322331, Length=270, Percent_Identity=31.4814814814815, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24642586, Length=309, Percent_Identity=28.1553398058252, Blast_Score=101, Evalue=6e-22,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 35272; Mature: 35141

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVQVPKLNTKDLLTLEELTQEEIISLIEFAIYLKNNKQEPLLQGKILGLIFDKHSTRT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCEEEEEEEECCCCCE
RVSFEAGMVQLGGHGMFLSGKEMQMGRGETVSDTAKVLSQYIDGIMIRTFSHADVEELAK
EEEEECCEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHH
ESSIPVINGLTDDHHPCQALADLMTIYEEVNTFKGIKLAYVGDGNNVCHSLLLASAKVGM
HCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHCCE
HMTVATPIGYEPNEEIVKKALAIAKETGAEIEILHNPELAVNEADFIYTDVWMSMGQEGE
EEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEECCCHHHHHHHHHHCCCCCC
EEKYTLFQPYQINKELVKHAKQTYRFLHCLPAHREEEVTGEIIDGPQSIVFEQAGNRLHA
CCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHCCHHHH
QKALLVSLFKNVEEPS
HHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STVQVPKLNTKDLLTLEELTQEEIISLIEFAIYLKNNKQEPLLQGKILGLIFDKHSTRT
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCEEEEEEEECCCCCE
RVSFEAGMVQLGGHGMFLSGKEMQMGRGETVSDTAKVLSQYIDGIMIRTFSHADVEELAK
EEEEECCEEEECCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHH
ESSIPVINGLTDDHHPCQALADLMTIYEEVNTFKGIKLAYVGDGNNVCHSLLLASAKVGM
HCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHCCE
HMTVATPIGYEPNEEIVKKALAIAKETGAEIEILHNPELAVNEADFIYTDVWMSMGQEGE
EEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEECCCHHHHHHHHHHCCCCCC
EEKYTLFQPYQINKELVKHAKQTYRFLHCLPAHREEEVTGEIIDGPQSIVFEQAGNRLHA
CCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHCCHHHH
QKALLVSLFKNVEEPS
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA