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The map label for this gene is polA [H]

Identifier: 52140922

GI number: 52140922

Start: 4440563

End: 4443238

Strand: Reverse

Name: polA [H]

Synonym: BCZK4328

Alternate gene names: 52140922

Gene position: 4443238-4440563 (Counterclockwise)

Preceding gene: 52140921

Following gene: 52140924

Centisome position: 83.82

GC content: 36.32

Gene sequence:

>2676_bases
GTGAAAAAAATCATTTCAAATTTGTATGGGGAGGTTTCTGATTTGGAAAAAAAGGTCGTATTAGTAGATGGTAATAATAT
CGCGTATCGTGCTTTCTTTGCACTACCGCTTTTAAATAACGACAAGGGTATACATACGAACGCAATTTACGGTTTTACAA
TGATGTTAATGAGAATATTAGAGGAAGAAAAACCGACGCATATGTTAGTTGCATTTGATGCGGGTAAAACAACGTTCCGT
CATAAAACGTATAGTGAGTATAAAGGAGGACGTCAAAAGACTCCACCTGAATTATCAGAGCAATTCCCGTTTATTCGTGA
GATGCTCGATGCATTTAATGTACCGCGTTATGAATTAGAAAATTATGAAGCGGATGACATTATGGGAACGCTAGCGAAAG
AAGCGAGTGAACAAGGAGCTAGTGTAAAAGTTATTTCAGGAGATAAAGATTTACTTCAACTTGTTTCTGATAACACGCTT
GTATGCATTCCTAGAAAAGGGATTACGGAAGTAGATGAATATACGGAAGAAGCTTTGTTTGAGAAATACAGCTTATCACC
GAAGCAAATTATTGATATGAAAGGTTTAATGGGAGACCAATCAGATAATATTCCAGGTGTACCAGGAGTTGGTGAAAAAA
CTGCGATTAAATTGTTAACACAGTTTGGAACGGTTGAAGAAGTGTATGAAAATATAGATCAAGTAAGCGGGAAGAAATTA
AAAGAAAAGCTGGAAGCAAATAAAGATCAAGCTCTTATGAGTAAAGATCTTGCAACCATTATTACAGACGCACCGATTAC
TGTGAATGTGGATGATATGGAGTATAAAGGATATGAAGCAAGTGATGTCATTCCAATGTTCGAGAATTTAGGATTTACAT
CTCTTTTAAACAAATTAGGTGTTACGCCAGAAGAAACGGCTCCAGCTGAATTAGATGATATTACATTTGATATTGTAGAA
GAAGTTACAGAAGAAATGCTTCAGCAAGATAGTGCGCTTATCGTTGAAGTACAAGAAGATAACTATCATAAAGCAGACAT
TCAAGGTTTCGGTATTCAAAATGAAAATGGATGTTACTTTATTCAGACAGATATTGCACTTAAATCAGATGCTTTTAAAG
AGTGGCTTGCAGATGGAGAAATGAGAAAGTATACATTTGATGCGAAACGTGCGATCGTTGCGCTGAAATGGAACGGTATA
GATATGCAAGGGATTGACTTTGATCTATTAATCGCTGCTTACTTACTTGATCCGGCTGATACAGATAAAGATTTCCGTAC
TGTAGCGAAAATGAAAGAAACGCATGCTGTGAAATCTGATGAAGAAGTTTACGGAAAAGGTGCGAAGCGTGCTGTTCCAG
AGTTAGAGATAGTAGCGGAGCATGTAGCTCGTAAAGTGCATGTATTATATGATGTAAAGCAAACATTCGTTGAAGAGTTA
GAAAAGAATGAGCAATATGAACTGTTTACAGAGTTAGAATTACCACTTGCACGTGTATTAGCTGATATGGAAGTAAAAGG
TGTAAAAGTTGATACAGAACGTCTTCGTAATATGGGAGAAGAACTTGCAGGTAGATTAAAGGAAATGGAACAGGAAATTT
ATAAACTGGCGGGAACAGAATTTAATATTAATTCACCGAAGCAGCTTGGTGTGATTCTGTTTGAGAATTTAAACTTACCG
GTTATTAAGAAGACGAAAACAGGTTATTCTACGTCAGCAGATGTATTAGACAAGCTGATGGATCACCATGAAATTATTCC
AAACATTTTACATTACCGTCAATTAGGAAAACTCAATTCAACTTATATTGAAGGTTTATTAAAGGTTGTACATGAAGATT
CATCTAAAATTCATACTCGTTTCAATCAAGTATTAACGCAAACGGGCCGATTAAGTTCAACGGATCCAAACTTGCAAAAT
ATCCCGATTCGATTAGAAGAAGGAAGAAAGATTCGTCAGGCATTCGTTCCATCAGAAGAAGGATGGATTATGTACGCGGC
CGATTATTCACAAATTGAACTTCGTGTATTAGCTCATATTGCAAATGATAAAGGGCTAGTTGAAGCGTTCCAACATGACA
TGGATATTCATACAAAAACAGCTATGGATGTATTTGGCGTTGAAAAAGATGAAGTAACTTCAAATATGAGACGACAAGCG
AAAGCTGTTAACTTCGGAATTGTGTATGGTATTAGTGATTATGGTCTTTCACAAAACTTAGGAATTACAAGAAAAGCAGC
AGCGGAATTTATTGAAAAGTATTTAGAAAGTTTCCCTGGTGTACAAGAATACATGGATGACATCGTAAAAGATGCGAAGC
AAAAAGGATATGTGGCTACATTATTAAATCGTCGCCGTTACATTCCGGAAATTACGAGTCGTAATTTCAACTTGCGTAGC
TTTGCTGAGCGTACAGCTATGAATACACCAATTCAAGGTACTGCAGCAGATATTATTAAAAAAGCGATGATTATTATGGC
AGATCGTTTAGAAGAAGAAGGATTACAAGCTCGTCTTCTATTGCAAGTACACGATGAATTAATATTTGAGGCACCAAAAG
AAGAAATTGAAAAATTAGAGAAGCTTGTACCAGAAGTAATGGAGCATGCAATTGAACTGGCAGTTCCACTGAAAGTTGAT
TATTCTTACGGTCCAACTTGGTACGACGCAAAATAA

Upstream 100 bases:

>100_bases
CAATATAAGTCTGTTTTATATATGAAAGAAAATAACCGTTATTTTTCTTCTCTCTATTAGTCATGTTAATATAGAGGGAG
GAAAGAAACGGTTTTTTTCT

Downstream 100 bases:

>100_bases
GGAAGTGATGAAATGCCTGAATTACCAGAGGTTGAAAACGTCAGACGGACACTTGAAAATCTTGTAACAGGAAAAACAAT
TGAAGACGTCATTGTTACCT

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 891; Mature: 891

Protein sequence:

>891_residues
MKKIISNLYGEVSDLEKKVVLVDGNNIAYRAFFALPLLNNDKGIHTNAIYGFTMMLMRILEEEKPTHMLVAFDAGKTTFR
HKTYSEYKGGRQKTPPELSEQFPFIREMLDAFNVPRYELENYEADDIMGTLAKEASEQGASVKVISGDKDLLQLVSDNTL
VCIPRKGITEVDEYTEEALFEKYSLSPKQIIDMKGLMGDQSDNIPGVPGVGEKTAIKLLTQFGTVEEVYENIDQVSGKKL
KEKLEANKDQALMSKDLATIITDAPITVNVDDMEYKGYEASDVIPMFENLGFTSLLNKLGVTPEETAPAELDDITFDIVE
EVTEEMLQQDSALIVEVQEDNYHKADIQGFGIQNENGCYFIQTDIALKSDAFKEWLADGEMRKYTFDAKRAIVALKWNGI
DMQGIDFDLLIAAYLLDPADTDKDFRTVAKMKETHAVKSDEEVYGKGAKRAVPELEIVAEHVARKVHVLYDVKQTFVEEL
EKNEQYELFTELELPLARVLADMEVKGVKVDTERLRNMGEELAGRLKEMEQEIYKLAGTEFNINSPKQLGVILFENLNLP
VIKKTKTGYSTSADVLDKLMDHHEIIPNILHYRQLGKLNSTYIEGLLKVVHEDSSKIHTRFNQVLTQTGRLSSTDPNLQN
IPIRLEEGRKIRQAFVPSEEGWIMYAADYSQIELRVLAHIANDKGLVEAFQHDMDIHTKTAMDVFGVEKDEVTSNMRRQA
KAVNFGIVYGISDYGLSQNLGITRKAAAEFIEKYLESFPGVQEYMDDIVKDAKQKGYVATLLNRRRYIPEITSRNFNLRS
FAERTAMNTPIQGTAADIIKKAMIIMADRLEEEGLQARLLLQVHDELIFEAPKEEIEKLEKLVPEVMEHAIELAVPLKVD
YSYGPTWYDAK

Sequences:

>Translated_891_residues
MKKIISNLYGEVSDLEKKVVLVDGNNIAYRAFFALPLLNNDKGIHTNAIYGFTMMLMRILEEEKPTHMLVAFDAGKTTFR
HKTYSEYKGGRQKTPPELSEQFPFIREMLDAFNVPRYELENYEADDIMGTLAKEASEQGASVKVISGDKDLLQLVSDNTL
VCIPRKGITEVDEYTEEALFEKYSLSPKQIIDMKGLMGDQSDNIPGVPGVGEKTAIKLLTQFGTVEEVYENIDQVSGKKL
KEKLEANKDQALMSKDLATIITDAPITVNVDDMEYKGYEASDVIPMFENLGFTSLLNKLGVTPEETAPAELDDITFDIVE
EVTEEMLQQDSALIVEVQEDNYHKADIQGFGIQNENGCYFIQTDIALKSDAFKEWLADGEMRKYTFDAKRAIVALKWNGI
DMQGIDFDLLIAAYLLDPADTDKDFRTVAKMKETHAVKSDEEVYGKGAKRAVPELEIVAEHVARKVHVLYDVKQTFVEEL
EKNEQYELFTELELPLARVLADMEVKGVKVDTERLRNMGEELAGRLKEMEQEIYKLAGTEFNINSPKQLGVILFENLNLP
VIKKTKTGYSTSADVLDKLMDHHEIIPNILHYRQLGKLNSTYIEGLLKVVHEDSSKIHTRFNQVLTQTGRLSSTDPNLQN
IPIRLEEGRKIRQAFVPSEEGWIMYAADYSQIELRVLAHIANDKGLVEAFQHDMDIHTKTAMDVFGVEKDEVTSNMRRQA
KAVNFGIVYGISDYGLSQNLGITRKAAAEFIEKYLESFPGVQEYMDDIVKDAKQKGYVATLLNRRRYIPEITSRNFNLRS
FAERTAMNTPIQGTAADIIKKAMIIMADRLEEEGLQARLLLQVHDELIFEAPKEEIEKLEKLVPEVMEHAIELAVPLKVD
YSYGPTWYDAK
>Mature_891_residues
MKKIISNLYGEVSDLEKKVVLVDGNNIAYRAFFALPLLNNDKGIHTNAIYGFTMMLMRILEEEKPTHMLVAFDAGKTTFR
HKTYSEYKGGRQKTPPELSEQFPFIREMLDAFNVPRYELENYEADDIMGTLAKEASEQGASVKVISGDKDLLQLVSDNTL
VCIPRKGITEVDEYTEEALFEKYSLSPKQIIDMKGLMGDQSDNIPGVPGVGEKTAIKLLTQFGTVEEVYENIDQVSGKKL
KEKLEANKDQALMSKDLATIITDAPITVNVDDMEYKGYEASDVIPMFENLGFTSLLNKLGVTPEETAPAELDDITFDIVE
EVTEEMLQQDSALIVEVQEDNYHKADIQGFGIQNENGCYFIQTDIALKSDAFKEWLADGEMRKYTFDAKRAIVALKWNGI
DMQGIDFDLLIAAYLLDPADTDKDFRTVAKMKETHAVKSDEEVYGKGAKRAVPELEIVAEHVARKVHVLYDVKQTFVEEL
EKNEQYELFTELELPLARVLADMEVKGVKVDTERLRNMGEELAGRLKEMEQEIYKLAGTEFNINSPKQLGVILFENLNLP
VIKKTKTGYSTSADVLDKLMDHHEIIPNILHYRQLGKLNSTYIEGLLKVVHEDSSKIHTRFNQVLTQTGRLSSTDPNLQN
IPIRLEEGRKIRQAFVPSEEGWIMYAADYSQIELRVLAHIANDKGLVEAFQHDMDIHTKTAMDVFGVEKDEVTSNMRRQA
KAVNFGIVYGISDYGLSQNLGITRKAAAEFIEKYLESFPGVQEYMDDIVKDAKQKGYVATLLNRRRYIPEITSRNFNLRS
FAERTAMNTPIQGTAADIIKKAMIIMADRLEEEGLQARLLLQVHDELIFEAPKEEIEKLEKLVPEVMEHAIELAVPLKVD
YSYGPTWYDAK

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=516, Percent_Identity=34.3023255813954, Blast_Score=267, Evalue=3e-71,
Organism=Homo sapiens, GI139394648, Length=634, Percent_Identity=26.6561514195584, Blast_Score=213, Evalue=6e-55,
Organism=Escherichia coli, GI1790294, Length=925, Percent_Identity=37.7297297297297, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI226510970, Length=226, Percent_Identity=30.5309734513274, Blast_Score=124, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI86563272, Length=410, Percent_Identity=28.5365853658537, Blast_Score=176, Evalue=5e-44,
Organism=Drosophila melanogaster, GI17933644, Length=419, Percent_Identity=32.9355608591885, Blast_Score=236, Evalue=7e-62,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 100994; Mature: 100994

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIISNLYGEVSDLEKKVVLVDGNNIAYRAFFALPLLNNDKGIHTNAIYGFTMMLMRIL
CHHHHHHHCCCHHCCCCEEEEEECCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHH
EEEKPTHMLVAFDAGKTTFRHKTYSEYKGGRQKTPPELSEQFPFIREMLDAFNVPRYELE
HCCCCCEEEEEEECCCCHHCCCCHHHHCCCCCCCCCCHHHCCHHHHHHHHHHCCCCHHHC
NYEADDIMGTLAKEASEQGASVKVISGDKDLLQLVSDNTLVCIPRKGITEVDEYTEEALF
CCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHH
EKYSLSPKQIIDMKGLMGDQSDNIPGVPGVGEKTAIKLLTQFGTVEEVYENIDQVSGKKL
HHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHH
KEKLEANKDQALMSKDLATIITDAPITVNVDDMEYKGYEASDVIPMFENLGFTSLLNKLG
HHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHCCCHHHHHHHHC
VTPEETAPAELDDITFDIVEEVTEEMLQQDSALIVEVQEDNYHKADIQGFGIQNENGCYF
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEECCCCCCCCEEE
IQTDIALKSDAFKEWLADGEMRKYTFDAKRAIVALKWNGIDMQGIDFDLLIAAYLLDPAD
EEECCHHHHHHHHHHHCCCCHHEEEECCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCC
TDKDFRTVAKMKETHAVKSDEEVYGKGAKRAVPELEIVAEHVARKVHVLYDVKQTFVEEL
CCHHHHHHHHHHHHHCCCCCHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKNEQYELFTELELPLARVLADMEVKGVKVDTERLRNMGEELAGRLKEMEQEIYKLAGTE
HCCCCEEHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FNINSPKQLGVILFENLNLPVIKKTKTGYSTSADVLDKLMDHHEIIPNILHYRQLGKLNS
CCCCCCHHCCEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCH
TYIEGLLKVVHEDSSKIHTRFNQVLTQTGRLSSTDPNLQNIPIRLEEGRKIRQAFVPSEE
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCHHHHHCCCCCC
GWIMYAADYSQIELRVLAHIANDKGLVEAFQHDMDIHTKTAMDVFGVEKDEVTSNMRRQA
CEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHH
KAVNFGIVYGISDYGLSQNLGITRKAAAEFIEKYLESFPGVQEYMDDIVKDAKQKGYVAT
HHHCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHH
LLNRRRYIPEITSRNFNLRSFAERTAMNTPIQGTAADIIKKAMIIMADRLEEEGLQARLL
HHHHHHCCCHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHH
LQVHDELIFEAPKEEIEKLEKLVPEVMEHAIELAVPLKVDYSYGPTWYDAK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKKIISNLYGEVSDLEKKVVLVDGNNIAYRAFFALPLLNNDKGIHTNAIYGFTMMLMRIL
CHHHHHHHCCCHHCCCCEEEEEECCCEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHH
EEEKPTHMLVAFDAGKTTFRHKTYSEYKGGRQKTPPELSEQFPFIREMLDAFNVPRYELE
HCCCCCEEEEEEECCCCHHCCCCHHHHCCCCCCCCCCHHHCCHHHHHHHHHHCCCCHHHC
NYEADDIMGTLAKEASEQGASVKVISGDKDLLQLVSDNTLVCIPRKGITEVDEYTEEALF
CCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHH
EKYSLSPKQIIDMKGLMGDQSDNIPGVPGVGEKTAIKLLTQFGTVEEVYENIDQVSGKKL
HHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHH
KEKLEANKDQALMSKDLATIITDAPITVNVDDMEYKGYEASDVIPMFENLGFTSLLNKLG
HHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHCCCHHHHHHHHC
VTPEETAPAELDDITFDIVEEVTEEMLQQDSALIVEVQEDNYHKADIQGFGIQNENGCYF
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEECCCCCCCCEEE
IQTDIALKSDAFKEWLADGEMRKYTFDAKRAIVALKWNGIDMQGIDFDLLIAAYLLDPAD
EEECCHHHHHHHHHHHCCCCHHEEEECCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCCC
TDKDFRTVAKMKETHAVKSDEEVYGKGAKRAVPELEIVAEHVARKVHVLYDVKQTFVEEL
CCHHHHHHHHHHHHHCCCCCHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKNEQYELFTELELPLARVLADMEVKGVKVDTERLRNMGEELAGRLKEMEQEIYKLAGTE
HCCCCEEHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
FNINSPKQLGVILFENLNLPVIKKTKTGYSTSADVLDKLMDHHEIIPNILHYRQLGKLNS
CCCCCCHHCCEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCH
TYIEGLLKVVHEDSSKIHTRFNQVLTQTGRLSSTDPNLQNIPIRLEEGRKIRQAFVPSEE
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCHHHHHCCCCCC
GWIMYAADYSQIELRVLAHIANDKGLVEAFQHDMDIHTKTAMDVFGVEKDEVTSNMRRQA
CEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHH
KAVNFGIVYGISDYGLSQNLGITRKAAAEFIEKYLESFPGVQEYMDDIVKDAKQKGYVAT
HHHCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHH
LLNRRRYIPEITSRNFNLRSFAERTAMNTPIQGTAADIIKKAMIIMADRLEEEGLQARLL
HHHHHHCCCHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHH
LQVHDELIFEAPKEEIEKLEKLVPEVMEHAIELAVPLKVDYSYGPTWYDAK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]