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The map label for this gene is bmr3 [H]

Identifier: 52140793

GI number: 52140793

Start: 4561663

End: 4563099

Strand: Direct

Name: bmr3 [H]

Synonym: BCZK4459

Alternate gene names: 52140793

Gene position: 4561663-4563099 (Clockwise)

Preceding gene: 52140794

Following gene: 52140788

Centisome position: 86.05

GC content: 38.76

Gene sequence:

>1437_bases
ATGAGAAAAAAAGTCATGATGTCTTTAATGCTAATGACGTTTCTTTCTGCAGTAGAAGGAACGATTGTTAGTACAGCAAT
CCCTCGTATAACGAGTGATTTGTCAGGCGTTGAACTTGTTAGCTGGGTTTACGCAATTTACATGCTTGCCACAGCGGTTT
CAACTCCAATATACGGAAAGCTAGCTGATTTATTTGGCCGTAAAAAAGTTTTACTTATTGGAGCTACAATCTTTTTAGTT
GGTTCTGCACTTTGCGGTGTCGTTACATCAATGGAACAATTAATCTTCTTCCGCGCTCTTCAAGGTATTGGTGCTGGTGC
TGTTATGCCAATTACTATGACAATCATTGGTGACTTATATAGCGAAGCGAAAGACCGCGCGAAAGCACAAGGCTGGATGA
GTGCCGTTTGGGGCGTCTCAGGTGTTATCGGACCATTAGTCGGCGGATTTTTAGTTGATTCTCTTTCTTGGCGCTACATC
TTCTTCTTAAACGTTCCATTTGGAATTATCGCTTGCTTAATGATTGCAATTTATTACAAAGAATCTATTAAGCCTGCTAA
GCACCATATCGACTATCTTGGTGCAACAGTATTCTCACTAAGTACGATTGCTCTACTATATGCATTATTAACAGGTAGCA
GTAAACAAAACTGGGGAGATGTAACAATTATCGGCCTATTAATCTTCGCCGTCGTTTCATTCATTATTTTCTTATTCATC
GAGAAAAAATCTCCGGAACCATTAATTCCGCTAGCACTTTTCTCTAACCGTACATTATCTACGATAAATATATTAACGCT
TATCGCTGGCGCAATGATTATTAGTATTACAATGTACCTTCCAATTTGGAGCCAAGGTGTATTAGGAAAAAATGCGACAG
AAGCCGGACTTATTTTAATGCCAATCCCTGTTATGTGGACATTTGGCGCTATTTTCTCTGGTAACTTAGTCGGCAAATTA
AAAACGAAACAAATCATTTTACTTGGAGCAAGCATTTTATCAGTCGCTACGTTCTTATTATTTACATTATCAACAAATTC
ACCATCGTTCCTTATTTATGTGGCAGTCGGATTATTCGGCTTAGGAATGGGGCTTGTAACACCGATTTACATGGTAACAA
TTCAAGCAGCCGTACCAGCTCATACACGTGGAACAGCAGTAGGTCTAAACACATTTATTAATACATTTAGCCAAACATTA
GGTGCTGCAATATTCGGTACAATTTTCAATACGATGATTCACGCACGTGGTATTAAAAACCTTGATCTTGTATCTGGGGG
ACACGGCGGAGCTACAGCAAACGTAGTAACTGAATCACAATCTGCATTAGCATCAAGTGTACACGTTATTTATATGAGCA
CTTTCGTATTGGCAGTATGTACATTAATTATCGCTTGGCTTTTATTAAAACCAGCTACACAAACAAGTGAGCAATAA

Upstream 100 bases:

>100_bases
GCTCCATGTAACTTCCCATTGCTTTTTTGTTGCTTTATATACCTTAGTACCTTTATATTTTATATTGTTGAATATTTGAA
AAAGGGTAAGGGGAAAACAT

Downstream 100 bases:

>100_bases
GAAAGAGGGCGACCATTTTGGTCATCCTCTTTTACTCTTCATTCTCCACAATATGTTTCAGCATGGACAATTTATTATCC
CAAAAACGTTCATAATACGA

Product: multidrug resistance protein B

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MRKKVMMSLMLMTFLSAVEGTIVSTAIPRITSDLSGVELVSWVYAIYMLATAVSTPIYGKLADLFGRKKVLLIGATIFLV
GSALCGVVTSMEQLIFFRALQGIGAGAVMPITMTIIGDLYSEAKDRAKAQGWMSAVWGVSGVIGPLVGGFLVDSLSWRYI
FFLNVPFGIIACLMIAIYYKESIKPAKHHIDYLGATVFSLSTIALLYALLTGSSKQNWGDVTIIGLLIFAVVSFIIFLFI
EKKSPEPLIPLALFSNRTLSTINILTLIAGAMIISITMYLPIWSQGVLGKNATEAGLILMPIPVMWTFGAIFSGNLVGKL
KTKQIILLGASILSVATFLLFTLSTNSPSFLIYVAVGLFGLGMGLVTPIYMVTIQAAVPAHTRGTAVGLNTFINTFSQTL
GAAIFGTIFNTMIHARGIKNLDLVSGGHGGATANVVTESQSALASSVHVIYMSTFVLAVCTLIIAWLLLKPATQTSEQ

Sequences:

>Translated_478_residues
MRKKVMMSLMLMTFLSAVEGTIVSTAIPRITSDLSGVELVSWVYAIYMLATAVSTPIYGKLADLFGRKKVLLIGATIFLV
GSALCGVVTSMEQLIFFRALQGIGAGAVMPITMTIIGDLYSEAKDRAKAQGWMSAVWGVSGVIGPLVGGFLVDSLSWRYI
FFLNVPFGIIACLMIAIYYKESIKPAKHHIDYLGATVFSLSTIALLYALLTGSSKQNWGDVTIIGLLIFAVVSFIIFLFI
EKKSPEPLIPLALFSNRTLSTINILTLIAGAMIISITMYLPIWSQGVLGKNATEAGLILMPIPVMWTFGAIFSGNLVGKL
KTKQIILLGASILSVATFLLFTLSTNSPSFLIYVAVGLFGLGMGLVTPIYMVTIQAAVPAHTRGTAVGLNTFINTFSQTL
GAAIFGTIFNTMIHARGIKNLDLVSGGHGGATANVVTESQSALASSVHVIYMSTFVLAVCTLIIAWLLLKPATQTSEQ
>Mature_478_residues
MRKKVMMSLMLMTFLSAVEGTIVSTAIPRITSDLSGVELVSWVYAIYMLATAVSTPIYGKLADLFGRKKVLLIGATIFLV
GSALCGVVTSMEQLIFFRALQGIGAGAVMPITMTIIGDLYSEAKDRAKAQGWMSAVWGVSGVIGPLVGGFLVDSLSWRYI
FFLNVPFGIIACLMIAIYYKESIKPAKHHIDYLGATVFSLSTIALLYALLTGSSKQNWGDVTIIGLLIFAVVSFIIFLFI
EKKSPEPLIPLALFSNRTLSTINILTLIAGAMIISITMYLPIWSQGVLGKNATEAGLILMPIPVMWTFGAIFSGNLVGKL
KTKQIILLGASILSVATFLLFTLSTNSPSFLIYVAVGLFGLGMGLVTPIYMVTIQAAVPAHTRGTAVGLNTFINTFSQTL
GAAIFGTIFNTMIHARGIKNLDLVSGGHGGATANVVTESQSALASSVHVIYMSTFVLAVCTLIIAWLLLKPATQTSEQ

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=430, Percent_Identity=26.7441860465116, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788710, Length=500, Percent_Identity=25.4, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI87081983, Length=413, Percent_Identity=27.6029055690073, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1790195, Length=362, Percent_Identity=27.9005524861878, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788392, Length=294, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790146, Length=161, Percent_Identity=27.9503105590062, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788509, Length=264, Percent_Identity=25.3787878787879, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI48994889, Length=202, Percent_Identity=29.7029702970297, Blast_Score=63, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=468, Percent_Identity=24.7863247863248, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323735, Length=309, Percent_Identity=27.831715210356, Blast_Score=113, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6319770, Length=368, Percent_Identity=26.3586956521739, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319783, Length=382, Percent_Identity=24.0837696335079, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321663, Length=463, Percent_Identity=22.6781857451404, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6324954, Length=410, Percent_Identity=22.9268292682927, Blast_Score=73, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=141, Percent_Identity=29.7872340425532, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323520, Length=292, Percent_Identity=26.7123287671233, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51111; Mature: 51111

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKVMMSLMLMTFLSAVEGTIVSTAIPRITSDLSGVELVSWVYAIYMLATAVSTPIYGK
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHH
LADLFGRKKVLLIGATIFLVGSALCGVVTSMEQLIFFRALQGIGAGAVMPITMTIIGDLY
HHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
SEAKDRAKAQGWMSAVWGVSGVIGPLVGGFLVDSLSWRYIFFLNVPFGIIACLMIAIYYK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHH
ESIKPAKHHIDYLGATVFSLSTIALLYALLTGSSKQNWGDVTIIGLLIFAVVSFIIFLFI
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EKKSPEPLIPLALFSNRTLSTINILTLIAGAMIISITMYLPIWSQGVLGKNATEAGLILM
CCCCCCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
PIPVMWTFGAIFSGNLVGKLKTKQIILLGASILSVATFLLFTLSTNSPSFLIYVAVGLFG
EHHHHHHHHHHHCCCCEEEECCCEEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LGMGLVTPIYMVTIQAAVPAHTRGTAVGLNTFINTFSQTLGAAIFGTIFNTMIHARGIKN
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LDLVSGGHGGATANVVTESQSALASSVHVIYMSTFVLAVCTLIIAWLLLKPATQTSEQ
CEEECCCCCCCEEHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRKKVMMSLMLMTFLSAVEGTIVSTAIPRITSDLSGVELVSWVYAIYMLATAVSTPIYGK
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHH
LADLFGRKKVLLIGATIFLVGSALCGVVTSMEQLIFFRALQGIGAGAVMPITMTIIGDLY
HHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
SEAKDRAKAQGWMSAVWGVSGVIGPLVGGFLVDSLSWRYIFFLNVPFGIIACLMIAIYYK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHH
ESIKPAKHHIDYLGATVFSLSTIALLYALLTGSSKQNWGDVTIIGLLIFAVVSFIIFLFI
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EKKSPEPLIPLALFSNRTLSTINILTLIAGAMIISITMYLPIWSQGVLGKNATEAGLILM
CCCCCCCCEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
PIPVMWTFGAIFSGNLVGKLKTKQIILLGASILSVATFLLFTLSTNSPSFLIYVAVGLFG
EHHHHHHHHHHHCCCCEEEECCCEEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LGMGLVTPIYMVTIQAAVPAHTRGTAVGLNTFINTFSQTLGAAIFGTIFNTMIHARGIKN
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LDLVSGGHGGATANVVTESQSALASSVHVIYMSTFVLAVCTLIIAWLLLKPATQTSEQ
CEEECCCCCCCEEHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]