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The map label for this gene is cls [H]

Identifier: 52140214

GI number: 52140214

Start: 5146120

End: 5147313

Strand: Direct

Name: cls [H]

Synonym: BCZK5046

Alternate gene names: 52140214

Gene position: 5146120-5147313 (Clockwise)

Preceding gene: 52140224

Following gene: 52140215

Centisome position: 97.08

GC content: 34.59

Gene sequence:

>1194_bases
ATGTTCTTCTTACCTCTCTTATTAATAGGTATTGCTCTCTGGATTGTAATCGATCTTTCTTATGGGAGAATTGTTCATTT
AAGGCGTGTACGTTCTCGTTCTTTCCCTTTACGTCAAAGTGATTTTCACCTTTATACATACGGAAAAGATTTGTATGACA
CTCTATTTACTGATATAAAACAGGCGAAACACCATGTGCATATTTTGTTCTTCATTGTAAAAAACGATAAAATCAGCCGA
GAATTTTTAAAGTTACTTATTAATAAGGCAAAGGAAGGAATTGAAGTAAGACTTTTACTCGATAGATTGGGAAGCCATCA
TTTATCAAACGAGGCCATTCGCTCCCTGCAAAAACATGGTGTCTCTTTTTCATTTTGTCACAAAGTAAAGTTTCCTTTTC
CTTTCTTTTCTGCAAATCAAAGAAACCATAGAAAAATTACAGTTATCGACGGTAAAATTGGTTACATTGGGGGATTTAAT
ATTGGTGAAGAGTATTTGGGACATAATGAGCGGTTAGGATTATGGAGGGACTATCATTTACGTCTTACAGGCGAAGGAGT
ACAAGATTTACAAAAGCAATTTTTACATGACTGGTTTGATGATACAAACCAAAATTTATTGGACGCTTCTCTTTATTTTC
CGAAACAGCAACCCGGTACTATCGAACATCAATTTATTCCAACTGATGGTGCCTATTTACAACATACTTTTTTACATTTA
ATTGAAGGTGCAAAAAAGGAACTCTTTATCGGTACACCCTATTTCATTCCTGGAAGGAAAATTATGAACGCATTATTAGA
AGCACGAAAACGCGGGGTTCAAATCACGATTCTCGTTCCAGAAAAAGCGGATCATGCCCTTGTTCGAGAAGCAAAATTCC
CGTATTGCCGAAAGTTAATACAAGCTGGATGTAATATTTACGCATTTCAACAAGGGTTTTTTCACGCTAAAGTTATTATA
GTTGATGACTACATTTGTGATATCGGAACTGCAAACTTTGATATGAGAAGTTTATATATTAACCATGAAATTAACTGCCT
TTTATATGATAAACATTTTATACAAACAGTAAGAAACAAATTCCATAAGGATCTAGGAAACGCATCATTACTTTCCTTTA
AAGATGTTAGCCCGCATTCTCTTATTGATAGAGGAAAAGAATGGATAGGAACAATACTCGCTTTCTTCCTATAG

Upstream 100 bases:

>100_bases
TATTCCCTACTGATTATTAGCCTGCAAATAACGGGATACTATGAATGTTTTATTTATAAAAAACGAATGGTGAAAAGACC
GTAAAGGAGGGAATTTACTC

Downstream 100 bases:

>100_bases
GAAAGATAATGCATAAAGGATGTGTATTCATTATGATTATGCGGTTCGGATATGTCTCACATGCAATGGCACTCTGGGAC
TGCTCTCCGGCTAAAACGAT

Product: cardiolipin synthetase domain-containing protein

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MFFLPLLLIGIALWIVIDLSYGRIVHLRRVRSRSFPLRQSDFHLYTYGKDLYDTLFTDIKQAKHHVHILFFIVKNDKISR
EFLKLLINKAKEGIEVRLLLDRLGSHHLSNEAIRSLQKHGVSFSFCHKVKFPFPFFSANQRNHRKITVIDGKIGYIGGFN
IGEEYLGHNERLGLWRDYHLRLTGEGVQDLQKQFLHDWFDDTNQNLLDASLYFPKQQPGTIEHQFIPTDGAYLQHTFLHL
IEGAKKELFIGTPYFIPGRKIMNALLEARKRGVQITILVPEKADHALVREAKFPYCRKLIQAGCNIYAFQQGFFHAKVII
VDDYICDIGTANFDMRSLYINHEINCLLYDKHFIQTVRNKFHKDLGNASLLSFKDVSPHSLIDRGKEWIGTILAFFL

Sequences:

>Translated_397_residues
MFFLPLLLIGIALWIVIDLSYGRIVHLRRVRSRSFPLRQSDFHLYTYGKDLYDTLFTDIKQAKHHVHILFFIVKNDKISR
EFLKLLINKAKEGIEVRLLLDRLGSHHLSNEAIRSLQKHGVSFSFCHKVKFPFPFFSANQRNHRKITVIDGKIGYIGGFN
IGEEYLGHNERLGLWRDYHLRLTGEGVQDLQKQFLHDWFDDTNQNLLDASLYFPKQQPGTIEHQFIPTDGAYLQHTFLHL
IEGAKKELFIGTPYFIPGRKIMNALLEARKRGVQITILVPEKADHALVREAKFPYCRKLIQAGCNIYAFQQGFFHAKVII
VDDYICDIGTANFDMRSLYINHEINCLLYDKHFIQTVRNKFHKDLGNASLLSFKDVSPHSLIDRGKEWIGTILAFFL
>Mature_397_residues
MFFLPLLLIGIALWIVIDLSYGRIVHLRRVRSRSFPLRQSDFHLYTYGKDLYDTLFTDIKQAKHHVHILFFIVKNDKISR
EFLKLLINKAKEGIEVRLLLDRLGSHHLSNEAIRSLQKHGVSFSFCHKVKFPFPFFSANQRNHRKITVIDGKIGYIGGFN
IGEEYLGHNERLGLWRDYHLRLTGEGVQDLQKQFLHDWFDDTNQNLLDASLYFPKQQPGTIEHQFIPTDGAYLQHTFLHL
IEGAKKELFIGTPYFIPGRKIMNALLEARKRGVQITILVPEKADHALVREAKFPYCRKLIQAGCNIYAFQQGFFHAKVII
VDDYICDIGTANFDMRSLYINHEINCLLYDKHFIQTVRNKFHKDLGNASLLSFKDVSPHSLIDRGKEWIGTILAFFL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=327, Percent_Identity=29.0519877675841, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1787007, Length=339, Percent_Identity=27.4336283185841, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87081826, Length=404, Percent_Identity=23.2673267326733, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 46229; Mature: 46229

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS50035 PLD ; PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFLPLLLIGIALWIVIDLSYGRIVHLRRVRSRSFPLRQSDFHLYTYGKDLYDTLFTDIK
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHH
QAKHHVHILFFIVKNDKISREFLKLLINKAKEGIEVRLLLDRLGSHHLSNEAIRSLQKHG
HHCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCHHHHHHHHHCC
VSFSFCHKVKFPFPFFSANQRNHRKITVIDGKIGYIGGFNIGEEYLGHNERLGLWRDYHL
CCEEEEEEECCCCCCCCCCCCCCCEEEEEECCEEEEECCCCCHHHCCCCCCCCCEEEEEE
RLTGEGVQDLQKQFLHDWFDDTNQNLLDASLYFPKQQPGTIEHQFIPTDGAYLQHTFLHL
EEECCCHHHHHHHHHHHHHCCCCCCEEEHHEECCCCCCCCCCEEEECCCCHHHHHHHHHH
IEGAKKELFIGTPYFIPGRKIMNALLEARKRGVQITILVPEKADHALVREAKFPYCRKLI
HHCCCCEEEECCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHCCCHHHHHHH
QAGCNIYAFQQGFFHAKVIIVDDYICDIGTANFDMRSLYINHEINCLLYDKHFIQTVRNK
HCCCCEEEEECCCEEEEEEEEECEEECCCCCCCEEEEEEECCCEEEEEECHHHHHHHHHH
FHKDLGNASLLSFKDVSPHSLIDRGKEWIGTILAFFL
HHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MFFLPLLLIGIALWIVIDLSYGRIVHLRRVRSRSFPLRQSDFHLYTYGKDLYDTLFTDIK
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHH
QAKHHVHILFFIVKNDKISREFLKLLINKAKEGIEVRLLLDRLGSHHLSNEAIRSLQKHG
HHCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCHHHHHHHHHCC
VSFSFCHKVKFPFPFFSANQRNHRKITVIDGKIGYIGGFNIGEEYLGHNERLGLWRDYHL
CCEEEEEEECCCCCCCCCCCCCCCEEEEEECCEEEEECCCCCHHHCCCCCCCCCEEEEEE
RLTGEGVQDLQKQFLHDWFDDTNQNLLDASLYFPKQQPGTIEHQFIPTDGAYLQHTFLHL
EEECCCHHHHHHHHHHHHHCCCCCCEEEHHEECCCCCCCCCCEEEECCCCHHHHHHHHHH
IEGAKKELFIGTPYFIPGRKIMNALLEARKRGVQITILVPEKADHALVREAKFPYCRKLI
HHCCCCEEEECCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHCCCHHHHHHH
QAGCNIYAFQQGFFHAKVIIVDDYICDIGTANFDMRSLYINHEINCLLYDKHFIQTVRNK
HCCCCEEEEECCCEEEEEEEEECEEECCCCCCCEEEEEEECCCEEEEEECHHHHHHHHHH
FHKDLGNASLLSFKDVSPHSLIDRGKEWIGTILAFFL
HHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]