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The map label for this gene is purA

Identifier: 51894448

GI number: 51894448

Start: 3541642

End: 3542940

Strand: Reverse

Name: purA

Synonym: STH3314

Alternate gene names: 51894448

Gene position: 3542940-3541642 (Counterclockwise)

Preceding gene: 51894449

Following gene: 51894447

Centisome position: 99.35

GC content: 68.75

Gene sequence:

>1299_bases
ATGCCAGTCGTAGTGGTGATGGGCGCGCAGTGGGGCGACGAGGGCAAGGGCAAGTTCGTCGACCTGCTGGCGGAGAGGGC
GCAGGTGGTCGTTCGCTCGACCGGCGGGTCCAACGCCGGACACACTGTGTGGGCCGGCGGTAGGCAGTACAAGCTGCATC
AGGTGCCTTCGGGCATCCTGTATCCCGGCACGCTGTGCGTGATCGGCCACGGCGTGGTGCTGGATCCGCCCAAGCTGCTG
GAGGAGATGGACCGGCTGGCGAGCCAGGGCGTCGATCTTTCCAGCCTGCGGATCAGCGGCGGCGCGCATATCGTCTTCCC
CTTCCACATCCGGCTGGACGAGGCGGAGGAGGACAGGAAGGGCGACCGGAAGATCGGCACCACACGGCGCGGCATCGGGC
CGGCCTATATGGACAAGTTCGCCCGGGTCGGCATCCGCCTCGTCGACATGCTCGACCGCGACGAGTTCCTGCCCAAGCTG
ACGGCCCTGGTCGAGGAGAAGAACCGCATCCTGGAGAAGGTGTACGGCCTGCCGGGCTTCACCGTGGAGGAGATCGCGGA
GCCGTACCTGGAGTACGCGGAGCGGCTGCGGCCGTACGTCGCGAACACGGTGGAGCTGGTCAACGATGCGATCGACGCAG
GCAAGAACGTGCTGTTCGAGGGCGCGCAGGGCCACCTGCTGGACATCGACTTCGGCACGTACCCGTACGTGACTGCGTCG
CACCCGATCGCCGCCGGAGCCATCATCGGCGCCGGCGTCGGGCCGACGAAGGTCTCCCGGGTGGTCGGCGTGGTCAAGGC
GTACACCTCCCGCGTCGGCGAGGGGCCCTTCCCGACCGAACTGCACGGCGAGGAGGCGCACCGCATCCGCGAGGAGGGCC
ACGAGTACGGCACGACCACCGGCCGACCGCGCCGCATCGGCTGGCTCGACCTGGTGATGGTGCGCTACGCCTGCCGGGTC
AGCGGCATCACTGACCTGGCCGTGCCGCACCTGGACACGCTGGCCAAGACGGGCCTGCCCACGCTGAAGGTCTGCGTCGG
CTACCGGATGCCCGACGGCACGGTGACCCGGGAGTTCCCCGTCGGCCTGAAGGCGCTCAGCCAGGTCGAACCCGTGTACG
AGGAACTGCCCAACTGGGAGTGGTCGGCGGAGATGAGCACCGCCAGGCAGTACGACGAGCTGCCGGAAGGCGCCCGGCGG
TACGTCCGGCTGATCGAGGACGTCACCGGCGTCCGCGTCTCCATCCTGGGGGTGGGCAGCGAACGGACGCAGGCGATCTA
CCGCTCCGCCATCTTTTGA

Upstream 100 bases:

>100_bases
CCGCGAGTCGGCGGATGATACACTTTGCCAGGGAATCGGCCAGCGCGGACCGGGGCCCCCGCCACGGGGGCCCGGCGCGT
TTCGTACGGGAGGTGCCGAC

Downstream 100 bases:

>100_bases
TGCGGACGTGACTCTGGAATGCGCGCGCGGGACGGCTGGCAGCCGTCCCGCGATTTTCAAGCCGAAACCGCCTTGCATTC
GGCAACGGGCGCCAGTATAA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MPVVVVMGAQWGDEGKGKFVDLLAERAQVVVRSTGGSNAGHTVWAGGRQYKLHQVPSGILYPGTLCVIGHGVVLDPPKLL
EEMDRLASQGVDLSSLRISGGAHIVFPFHIRLDEAEEDRKGDRKIGTTRRGIGPAYMDKFARVGIRLVDMLDRDEFLPKL
TALVEEKNRILEKVYGLPGFTVEEIAEPYLEYAERLRPYVANTVELVNDAIDAGKNVLFEGAQGHLLDIDFGTYPYVTAS
HPIAAGAIIGAGVGPTKVSRVVGVVKAYTSRVGEGPFPTELHGEEAHRIREEGHEYGTTTGRPRRIGWLDLVMVRYACRV
SGITDLAVPHLDTLAKTGLPTLKVCVGYRMPDGTVTREFPVGLKALSQVEPVYEELPNWEWSAEMSTARQYDELPEGARR
YVRLIEDVTGVRVSILGVGSERTQAIYRSAIF

Sequences:

>Translated_432_residues
MPVVVVMGAQWGDEGKGKFVDLLAERAQVVVRSTGGSNAGHTVWAGGRQYKLHQVPSGILYPGTLCVIGHGVVLDPPKLL
EEMDRLASQGVDLSSLRISGGAHIVFPFHIRLDEAEEDRKGDRKIGTTRRGIGPAYMDKFARVGIRLVDMLDRDEFLPKL
TALVEEKNRILEKVYGLPGFTVEEIAEPYLEYAERLRPYVANTVELVNDAIDAGKNVLFEGAQGHLLDIDFGTYPYVTAS
HPIAAGAIIGAGVGPTKVSRVVGVVKAYTSRVGEGPFPTELHGEEAHRIREEGHEYGTTTGRPRRIGWLDLVMVRYACRV
SGITDLAVPHLDTLAKTGLPTLKVCVGYRMPDGTVTREFPVGLKALSQVEPVYEELPNWEWSAEMSTARQYDELPEGARR
YVRLIEDVTGVRVSILGVGSERTQAIYRSAIF
>Mature_431_residues
PVVVVMGAQWGDEGKGKFVDLLAERAQVVVRSTGGSNAGHTVWAGGRQYKLHQVPSGILYPGTLCVIGHGVVLDPPKLLE
EMDRLASQGVDLSSLRISGGAHIVFPFHIRLDEAEEDRKGDRKIGTTRRGIGPAYMDKFARVGIRLVDMLDRDEFLPKLT
ALVEEKNRILEKVYGLPGFTVEEIAEPYLEYAERLRPYVANTVELVNDAIDAGKNVLFEGAQGHLLDIDFGTYPYVTASH
PIAAGAIIGAGVGPTKVSRVVGVVKAYTSRVGEGPFPTELHGEEAHRIREEGHEYGTTTGRPRRIGWLDLVMVRYACRVS
GITDLAVPHLDTLAKTGLPTLKVCVGYRMPDGTVTREFPVGLKALSQVEPVYEELPNWEWSAEMSTARQYDELPEGARRY
VRLIEDVTGVRVSILGVGSERTQAIYRSAIF

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=431, Percent_Identity=42.92343387471, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI34577063, Length=433, Percent_Identity=42.2632794457275, Blast_Score=318, Evalue=6e-87,
Organism=Homo sapiens, GI40316944, Length=399, Percent_Identity=41.1027568922306, Blast_Score=277, Evalue=2e-74,
Organism=Escherichia coli, GI1790620, Length=424, Percent_Identity=43.8679245283019, Blast_Score=353, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI25146553, Length=437, Percent_Identity=43.2494279176201, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI25146555, Length=438, Percent_Identity=43.1506849315069, Blast_Score=322, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6324109, Length=438, Percent_Identity=39.2694063926941, Blast_Score=314, Evalue=2e-86,
Organism=Drosophila melanogaster, GI21356033, Length=429, Percent_Identity=42.8904428904429, Blast_Score=333, Evalue=1e-91,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_SYMTH (Q67J55)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_077139.1
- ProteinModelPortal:   Q67J55
- SMR:   Q67J55
- GeneID:   2981392
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH3314
- NMPDR:   fig|292459.1.peg.3182
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- BioCyc:   STHE292459:STH3314-MONOMER
- BRENDA:   6.3.4.4
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47445; Mature: 47314

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVVVVMGAQWGDEGKGKFVDLLAERAQVVVRSTGGSNAGHTVWAGGRQYKLHQVPSGIL
CCEEEEECCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEECCCEEEEEECCCCCC
YPGTLCVIGHGVVLDPPKLLEEMDRLASQGVDLSSLRISGGAHIVFPFHIRLDEAEEDRK
CCCEEEEEECCEEECCHHHHHHHHHHHHCCCCHHEEEECCCEEEEEEEEEEECCCHHHCC
GDRKIGTTRRGIGPAYMDKFARVGIRLVDMLDRDEFLPKLTALVEEKNRILEKVYGLPGF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TVEEIAEPYLEYAERLRPYVANTVELVNDAIDAGKNVLFEGAQGHLLDIDFGTYPYVTAS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCEECC
HPIAAGAIIGAGVGPTKVSRVVGVVKAYTSRVGEGPFPTELHGEEAHRIREEGHEYGTTT
CCCHHHHHEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
GRPRRIGWLDLVMVRYACRVSGITDLAVPHLDTLAKTGLPTLKVCVGYRMPDGTVTREFP
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEHHCCEECCCCCCCCCCC
VGLKALSQVEPVYEELPNWEWSAEMSTARQYDELPEGARRYVRLIEDVTGVRVSILGVGS
HHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCC
ERTQAIYRSAIF
HHHHHHHHHHCC
>Mature Secondary Structure 
PVVVVMGAQWGDEGKGKFVDLLAERAQVVVRSTGGSNAGHTVWAGGRQYKLHQVPSGIL
CEEEEECCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEECCCEEEEEECCCCCC
YPGTLCVIGHGVVLDPPKLLEEMDRLASQGVDLSSLRISGGAHIVFPFHIRLDEAEEDRK
CCCEEEEEECCEEECCHHHHHHHHHHHHCCCCHHEEEECCCEEEEEEEEEEECCCHHHCC
GDRKIGTTRRGIGPAYMDKFARVGIRLVDMLDRDEFLPKLTALVEEKNRILEKVYGLPGF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TVEEIAEPYLEYAERLRPYVANTVELVNDAIDAGKNVLFEGAQGHLLDIDFGTYPYVTAS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCEECC
HPIAAGAIIGAGVGPTKVSRVVGVVKAYTSRVGEGPFPTELHGEEAHRIREEGHEYGTTT
CCCHHHHHEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
GRPRRIGWLDLVMVRYACRVSGITDLAVPHLDTLAKTGLPTLKVCVGYRMPDGTVTREFP
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEHHCCEECCCCCCCCCCC
VGLKALSQVEPVYEELPNWEWSAEMSTARQYDELPEGARRYVRLIEDVTGVRVSILGVGS
HHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCC
ERTQAIYRSAIF
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA