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The map label for this gene is hydN2 [H]

Identifier: 51894428

GI number: 51894428

Start: 3518337

End: 3518921

Strand: Reverse

Name: hydN2 [H]

Synonym: STH3294

Alternate gene names: 51894428

Gene position: 3518921-3518337 (Counterclockwise)

Preceding gene: 51894429

Following gene: 51894427

Centisome position: 98.68

GC content: 67.52

Gene sequence:

>585_bases
ATGTGCGCGCTCAACGCCTTTGTCGTGGCGGATCCCCATAAGTGTATCGGCTGCAAGGTCTGTGAGGTTTCCTGCTTCGT
GGTCCACTCCGACGTGCCCGTGAAGACGGTCGGCCAGGTGACCACGCCCATCATCCCCCGGCTGTACCTGGTGCACACTG
CCGAGGTCACCATGCCGGTGCAGTGCCGGCACTGCGAGGACGCTCCCTGCGCCAACGCCTGCCCGGTGGGCGCGATCACG
CAGAAGGACGGCATCATCGACGTCGACCAGGAGACCTGCATCGGCTGCAAGACCTGCGTCCTGGCCTGTCCGTTCGGGGC
CATGGAGATGGTGCCCTACTACAAGAACGGCGAGCAGGTGAAGCAGCTGCGGCTGACCGAGGAGACCGGCGAGGGGCCGG
TGCTGCAGGATGTGTTCGTGGCCAGCAAGTGCGACCGGTGCATCGGGGTCGAGGGCGGCCCGGCCTGCGTCGCCAACTGC
CCGCGGGAGGCGCTGCAGGTCGTCCGACCCGATGAGCTGCGCCGGACCCGGAACCTGGAGGCCGCCCTGGCCGTGCTCGA
GTCCGTGAAGAACGTCGTCGGGTAG

Upstream 100 bases:

>100_bases
AGCGCCCTGGACCCGGTCTCGAAGACCCCGGAGTACAAGGTCTGTGCCGTACGGGTAGAAAAGGTCAGCGCCTAAACTTC
TCGAGAGGAGGCCTGCGACG

Downstream 100 bases:

>100_bases
GAGGGACCGCCGTGATTTTCATCGATCAGGAACTCTGCACCGGTTGCCGTCGCTGTGCCGAAGTCTGCCCGGTGGACGCC
ATCGTCGGCGAGCCGGGGAA

Product: electron transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MCALNAFVVADPHKCIGCKVCEVSCFVVHSDVPVKTVGQVTTPIIPRLYLVHTAEVTMPVQCRHCEDAPCANACPVGAIT
QKDGIIDVDQETCIGCKTCVLACPFGAMEMVPYYKNGEQVKQLRLTEETGEGPVLQDVFVASKCDRCIGVEGGPACVANC
PREALQVVRPDELRRTRNLEAALAVLESVKNVVG

Sequences:

>Translated_194_residues
MCALNAFVVADPHKCIGCKVCEVSCFVVHSDVPVKTVGQVTTPIIPRLYLVHTAEVTMPVQCRHCEDAPCANACPVGAIT
QKDGIIDVDQETCIGCKTCVLACPFGAMEMVPYYKNGEQVKQLRLTEETGEGPVLQDVFVASKCDRCIGVEGGPACVANC
PREALQVVRPDELRRTRNLEAALAVLESVKNVVG
>Mature_194_residues
MCALNAFVVADPHKCIGCKVCEVSCFVVHSDVPVKTVGQVTTPIIPRLYLVHTAEVTMPVQCRHCEDAPCANACPVGAIT
QKDGIIDVDQETCIGCKTCVLACPFGAMEMVPYYKNGEQVKQLRLTEETGEGPVLQDVFVASKCDRCIGVEGGPACVANC
PREALQVVRPDELRRTRNLEAALAVLESVKNVVG

Specific function: Electron transport from formate to hydrogen [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789067, Length=173, Percent_Identity=43.3526011560694, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1788811, Length=165, Percent_Identity=41.2121212121212, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI2367245, Length=174, Percent_Identity=40.8045977011494, Blast_Score=125, Evalue=1e-30,
Organism=Escherichia coli, GI87082114, Length=195, Percent_Identity=38.974358974359, Blast_Score=122, Evalue=1e-29,
Organism=Escherichia coli, GI87082179, Length=175, Percent_Identity=36.5714285714286, Blast_Score=114, Evalue=3e-27,
Organism=Escherichia coli, GI1789079, Length=182, Percent_Identity=36.2637362637363, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI87082180, Length=177, Percent_Identity=32.7683615819209, Blast_Score=103, Evalue=6e-24,
Organism=Escherichia coli, GI1787122, Length=196, Percent_Identity=30.6122448979592, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI1787872, Length=196, Percent_Identity=29.5918367346939, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI226510944, Length=170, Percent_Identity=31.7647058823529, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI2367345, Length=171, Percent_Identity=29.2397660818713, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1787963, Length=117, Percent_Identity=35.8974358974359, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 20847; Mature: 20847

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
10.8 %Cys+Met (Translated Protein)
8.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
10.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCALNAFVVADPHKCIGCKVCEVSCFVVHSDVPVKTVGQVTTPIIPRLYLVHTAEVTMPV
CCCCCEEEEECCCHHCCEEEEEEEEEEEECCCCCHHHHHHHCCHHCEEEEEEECCEEECE
QCRHCEDAPCANACPVGAITQKDGIIDVDQETCIGCKTCVLACPFGAMEMVPYYKNGEQV
EECCCCCCCCCCCCCCCCEECCCCEEECCCHHHHHHHHHHHCCCCCHHHHCCCCCCCHHH
KQLRLTEETGEGPVLQDVFVASKCDRCIGVEGGPACVANCPREALQVVRPDELRRTRNLE
HHEEECCCCCCCCCHHHHHHHHHCCHHCCCCCCCHHHHCCCHHHHHHCCCHHHHHHHHHH
AALAVLESVKNVVG
HHHHHHHHHHHHCC
>Mature Secondary Structure
MCALNAFVVADPHKCIGCKVCEVSCFVVHSDVPVKTVGQVTTPIIPRLYLVHTAEVTMPV
CCCCCEEEEECCCHHCCEEEEEEEEEEEECCCCCHHHHHHHCCHHCEEEEEEECCEEECE
QCRHCEDAPCANACPVGAITQKDGIIDVDQETCIGCKTCVLACPFGAMEMVPYYKNGEQV
EECCCCCCCCCCCCCCCCEECCCCEEECCCHHHHHHHHHHHCCCCCHHHHCCCCCCCHHH
KQLRLTEETGEGPVLQDVFVASKCDRCIGVEGGPACVANCPREALQVVRPDELRRTRNLE
HHEEECCCCCCCCCHHHHHHHHHCCHHCCCCCCCHHHHCCCHHHHHHCCCHHHHHHHHHH
AALAVLESVKNVVG
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]