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The map label for this gene is rlmI [H]

Identifier: 51894272

GI number: 51894272

Start: 3337969

End: 3339141

Strand: Reverse

Name: rlmI [H]

Synonym: STH3137

Alternate gene names: 51894272

Gene position: 3339141-3337969 (Counterclockwise)

Preceding gene: 51894275

Following gene: 51894271

Centisome position: 93.63

GC content: 68.2

Gene sequence:

>1173_bases
ATGGCGGTTGTGCAGTTGGCCCGGGGCCGCCACCGGCGGGTCCAGGCGGGTCATCCGTGGATTTTTCAGGGCGAGGTGGA
GGAGATCCGGGGCGAGTTCGCTCCCGGGGACGTGGTGACCGTGGTGGACTTCCGGGGCCGGCTGCTGGGCAAGGGGTACA
TCAACCCCGCGTCGCAGATCCTCGTCCGCATGCTGACGGCCCACGATGAGCCCGTCGACCGGGAGTTCTTCCGGCGCCGC
CTCGAACGGGCCTGGGCGTACCGGCAGAAGCTGCTTCCGGACACGGGGGCCTGCCGGGTGGTCTTCGGCGAGGCGGACTT
CCTGCCCGGATTGATCGTGGACAAGTTCGGCGACATCCTGGTGGTGCAGACCCTCGCGCTCGGCATCGACAAATGGCTGC
CGGATATTGTCGATATCCTTGTCGACCTGCTGCGGCCGCGGGGCATCTACGAGCGGAACGACGTGCCGGTGCGCCGTCTG
GAGGGGTTGGAGGAGCGTAAGGGCTTCCTGCGGGGCGAGTTCGACCCGCGGATCACGATTGTCGAAAACGGGCTGGAGAT
CGCGGTGGACGTGGCCGAGGGCCAGAAGACCGGGTACTACCTGGACCAGCGGGAGAACCGCCACGCCGTGCGCCGCTACG
CGGCGGGCGGGCGCGTGCTGGACTGCTTCTGCAACGTGGGCGGCTTTGCGCTCAACGCGGCCGCAGCCGGCGCCAGGGAG
GTCGTCGCGGTGGACGCCTCCGCGGAGGCGATCGCCGCCGCGGAGGAGAACGCCGCGCGCAACGGCCTGCGCGACCGGAT
CCGGTTCCAGGTGGGCAACGCCTTCGACATTCTGCGACAGATGGAGGCGCAGAGGGAACGGTTCGACGTCATCGTGCTGG
ATCCGCCCGCCTTCGCCAAGAACAAGGCAGCACTGGAAGGCGCGGCGCGGGGATACAAGGAGATCAACCTGCGCGCCCTC
CGTATGCTGACCGAGGGCGGGTTCCTCGTCACCTGCTCCTGCTCGTACCACATGACGCCGGACCTGTTCAAGGCCGTGGT
GGCGGAGGCGGCCCAGGACGCCCGCCGGCGCCTCAGGCTGGTCGAGGAGCGGGCACAGGCCGTCGACCACCCGATCGTGG
TCGGGTACGACGAGAGCCACTACCTGAAGTGTCTCATCTACGAAGTCATTTAA

Upstream 100 bases:

>100_bases
GGTAGGATGCCCGGCTGCGTTGGCTTCCATGACGCGCCGGTGTCGGGGGCAGGCTAACGTTGCGCTGGTGCCGAATCTGT
CTGTGGAGGGAGATGTGTCC

Downstream 100 bases:

>100_bases
CGGGCGTCCCGGCCGTTTCTCCAGGGAGGTTCCGAACGTCAAATAAGGTCAGAGGGAGTCTCCGGCGGCCCCTGGCGGGG
AAAAATGCGGTAGAGGCGCA

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MAVVQLARGRHRRVQAGHPWIFQGEVEEIRGEFAPGDVVTVVDFRGRLLGKGYINPASQILVRMLTAHDEPVDREFFRRR
LERAWAYRQKLLPDTGACRVVFGEADFLPGLIVDKFGDILVVQTLALGIDKWLPDIVDILVDLLRPRGIYERNDVPVRRL
EGLEERKGFLRGEFDPRITIVENGLEIAVDVAEGQKTGYYLDQRENRHAVRRYAAGGRVLDCFCNVGGFALNAAAAGARE
VVAVDASAEAIAAAEENAARNGLRDRIRFQVGNAFDILRQMEAQRERFDVIVLDPPAFAKNKAALEGAARGYKEINLRAL
RMLTEGGFLVTCSCSYHMTPDLFKAVVAEAAQDARRRLRLVEERAQAVDHPIVVGYDESHYLKCLIYEVI

Sequences:

>Translated_390_residues
MAVVQLARGRHRRVQAGHPWIFQGEVEEIRGEFAPGDVVTVVDFRGRLLGKGYINPASQILVRMLTAHDEPVDREFFRRR
LERAWAYRQKLLPDTGACRVVFGEADFLPGLIVDKFGDILVVQTLALGIDKWLPDIVDILVDLLRPRGIYERNDVPVRRL
EGLEERKGFLRGEFDPRITIVENGLEIAVDVAEGQKTGYYLDQRENRHAVRRYAAGGRVLDCFCNVGGFALNAAAAGARE
VVAVDASAEAIAAAEENAARNGLRDRIRFQVGNAFDILRQMEAQRERFDVIVLDPPAFAKNKAALEGAARGYKEINLRAL
RMLTEGGFLVTCSCSYHMTPDLFKAVVAEAAQDARRRLRLVEERAQAVDHPIVVGYDESHYLKCLIYEVI
>Mature_389_residues
AVVQLARGRHRRVQAGHPWIFQGEVEEIRGEFAPGDVVTVVDFRGRLLGKGYINPASQILVRMLTAHDEPVDREFFRRRL
ERAWAYRQKLLPDTGACRVVFGEADFLPGLIVDKFGDILVVQTLALGIDKWLPDIVDILVDLLRPRGIYERNDVPVRRLE
GLEERKGFLRGEFDPRITIVENGLEIAVDVAEGQKTGYYLDQRENRHAVRRYAAGGRVLDCFCNVGGFALNAAAAGAREV
VAVDASAEAIAAAEENAARNGLRDRIRFQVGNAFDILRQMEAQRERFDVIVLDPPAFAKNKAALEGAARGYKEINLRALR
MLTEGGFLVTCSCSYHMTPDLFKAVVAEAAQDARRRLRLVEERAQAVDHPIVVGYDESHYLKCLIYEVI

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=383, Percent_Identity=43.0809399477807, Blast_Score=314, Evalue=7e-87,
Organism=Escherichia coli, GI1787181, Length=195, Percent_Identity=27.1794871794872, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43755; Mature: 43624

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVQLARGRHRRVQAGHPWIFQGEVEEIRGEFAPGDVVTVVDFRGRLLGKGYINPASQI
CCCEEHHCCCCCCCCCCCCEEECCCHHHHCCCCCCCCEEEEEECCCCEECCCCCCHHHHH
LVRMLTAHDEPVDREFFRRRLERAWAYRQKLLPDTGACRVVFGEADFLPGLIVDKFGDIL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHCCHHH
VVQTLALGIDKWLPDIVDILVDLLRPRGIYERNDVPVRRLEGLEERKGFLRGEFDPRITI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHHCCCCCCCCCCEEEE
VENGLEIAVDVAEGQKTGYYLDQRENRHAVRRYAAGGRVLDCFCNVGGFALNAAAAGARE
EECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCEEHHHHCCCCE
VVAVDASAEAIAAAEENAARNGLRDRIRFQVGNAFDILRQMEAQRERFDVIVLDPPAFAK
EEEECCCHHHHHHHHHHHHHCCHHHHHHEECCCHHHHHHHHHHHHHHCCEEEECCCCCCC
NKAALEGAARGYKEINLRALRMLTEGGFLVTCSCSYHMTPDLFKAVVAEAAQDARRRLRL
CHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
VEERAQAVDHPIVVGYDESHYLKCLIYEVI
HHHHHHHCCCCEEEECCCCCHHHHEEEECC
>Mature Secondary Structure 
AVVQLARGRHRRVQAGHPWIFQGEVEEIRGEFAPGDVVTVVDFRGRLLGKGYINPASQI
CCEEHHCCCCCCCCCCCCEEECCCHHHHCCCCCCCCEEEEEECCCCEECCCCCCHHHHH
LVRMLTAHDEPVDREFFRRRLERAWAYRQKLLPDTGACRVVFGEADFLPGLIVDKFGDIL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHCCHHH
VVQTLALGIDKWLPDIVDILVDLLRPRGIYERNDVPVRRLEGLEERKGFLRGEFDPRITI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHHCCCCCCCCCCEEEE
VENGLEIAVDVAEGQKTGYYLDQRENRHAVRRYAAGGRVLDCFCNVGGFALNAAAAGARE
EECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCEEHHHHCCCCE
VVAVDASAEAIAAAEENAARNGLRDRIRFQVGNAFDILRQMEAQRERFDVIVLDPPAFAK
EEEECCCHHHHHHHHHHHHHCCHHHHHHEECCCHHHHHHHHHHHHHHCCEEEECCCCCCC
NKAALEGAARGYKEINLRALRMLTEGGFLVTCSCSYHMTPDLFKAVVAEAAQDARRRLRL
CHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
VEERAQAVDHPIVVGYDESHYLKCLIYEVI
HHHHHHHCCCCEEEECCCCCHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA