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The map label for this gene is ispD

Identifier: 51894258

GI number: 51894258

Start: 3322973

End: 3323683

Strand: Reverse

Name: ispD

Synonym: STH3123

Alternate gene names: 51894258

Gene position: 3323683-3322973 (Counterclockwise)

Preceding gene: 51894259

Following gene: 51894257

Centisome position: 93.2

GC content: 71.73

Gene sequence:

>711_bases
GTGAGCATTCATGCAGGGGGGCTGCAGGTCCCGGTCAGCGTGATCATCCCGGCCGCAGGGTCGGGCAGGCGCATGGGGGG
AGGAACTGCCAAGCAGTTCCTCCCCCTCCGCGGCGAGCCGGTGCTGGTGCGCACCGTCAGGCGGTTCAGCGAGTGCCCCC
TGGTGGACGAGATCGTCATCGCCGCGGGCGACGTGGAGGCGACCCGCGCTCTGGTGGGCCACATGCCCAAGGTGACGCGC
ATCGTCCAGGGCGGCGCGGAGCGGCAGGATTCGGTGTGGGCCGCCCTGCAGGCGGTCCATTCCCGCCCCCGCATCGTCGC
CGTCCACGACGCTGCCCGCCCCCTGCTCACCGCCGACGTGCTGAAGGGCGTGCTTCAGGCGGCGGCCGACCATCCGGCCC
AGGTGGTCGCGGTGCCCGTGCAGGATACCATCAAGCAGGTGGGGCCGGACGGCGTGGTGGTGGCGACGCCGGACCGGTCG
ATGCTGTGGGCGGTGCAGACCCCGCAGGTGTTCTGGGCTGATGTGCTGGTACGGGCCTTCCGCCAGGCGATCGCGGACGG
TTTCCTGGGCACGGACGACGCGTCGCTGGTGGAGCGCATCGGCGTGCCCGTGCGGGTCTACCGGGGCCATCCGGGCAACC
TCAAGCTGACCACGCCGACGGACTTCCGCCTGGCGGAGCTGCTGCTGGAGGAGGAGGCGCGACAATGCTGA

Upstream 100 bases:

>100_bases
TCGGAGACACCATCGACGTGCTGGTCACGTCGGTCATCCAGACGGCAGCCGGCCGCATGATCTTCGCCAAGCCGAAGATG
ACGGTGGAGAAGGTGGCGCC

Downstream 100 bases:

>100_bases
TCGGCTTCGGTTACGACGTTCACCGGCTGGTCCCCGGGAGGCCGCTGGTCCTGGGCGGCGTGGAGATCCCCAGCAAGCGG
GGCTTGGAGGGGCACTCCGA

Product: 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MSIHAGGLQVPVSVIIPAAGSGRRMGGGTAKQFLPLRGEPVLVRTVRRFSECPLVDEIVIAAGDVEATRALVGHMPKVTR
IVQGGAERQDSVWAALQAVHSRPRIVAVHDAARPLLTADVLKGVLQAAADHPAQVVAVPVQDTIKQVGPDGVVVATPDRS
MLWAVQTPQVFWADVLVRAFRQAIADGFLGTDDASLVERIGVPVRVYRGHPGNLKLTTPTDFRLAELLLEEEARQC

Sequences:

>Translated_236_residues
MSIHAGGLQVPVSVIIPAAGSGRRMGGGTAKQFLPLRGEPVLVRTVRRFSECPLVDEIVIAAGDVEATRALVGHMPKVTR
IVQGGAERQDSVWAALQAVHSRPRIVAVHDAARPLLTADVLKGVLQAAADHPAQVVAVPVQDTIKQVGPDGVVVATPDRS
MLWAVQTPQVFWADVLVRAFRQAIADGFLGTDDASLVERIGVPVRVYRGHPGNLKLTTPTDFRLAELLLEEEARQC
>Mature_235_residues
SIHAGGLQVPVSVIIPAAGSGRRMGGGTAKQFLPLRGEPVLVRTVRRFSECPLVDEIVIAAGDVEATRALVGHMPKVTRI
VQGGAERQDSVWAALQAVHSRPRIVAVHDAARPLLTADVLKGVLQAAADHPAQVVAVPVQDTIKQVGPDGVVVATPDRSM
LWAVQTPQVFWADVLVRAFRQAIADGFLGTDDASLVERIGVPVRVYRGHPGNLKLTTPTDFRLAELLLEEEARQC

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=237, Percent_Identity=28.2700421940928, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI157671913, Length=131, Percent_Identity=30.5343511450382, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1789104, Length=231, Percent_Identity=35.9307359307359, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_SYMTH (Q67JP5)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_076949.1
- ProteinModelPortal:   Q67JP5
- SMR:   Q67JP5
- GeneID:   2979926
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH3123
- HOGENOM:   HBG672839
- OMA:   PSNIKVT
- BioCyc:   STHE292459:STH3123-MONOMER
- BRENDA:   2.7.7.60
- HAMAP:   MF_00108
- InterPro:   IPR001228
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25240; Mature: 25108

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIHAGGLQVPVSVIIPAAGSGRRMGGGTAKQFLPLRGEPVLVRTVRRFSECPLVDEIVI
CCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCHHHHEE
AAGDVEATRALVGHMPKVTRIVQGGAERQDSVWAALQAVHSRPRIVAVHDAARPLLTADV
ECCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
LKGVLQAAADHPAQVVAVPVQDTIKQVGPDGVVVATPDRSMLWAVQTPQVFWADVLVRAF
HHHHHHHHCCCCCEEEEECHHHHHHHCCCCCEEEECCCCCEEEEECCCHHHHHHHHHHHH
RQAIADGFLGTDDASLVERIGVPVRVYRGHPGNLKLTTPTDFRLAELLLEEEARQC
HHHHHCCCCCCCHHHHHHHCCCCEEEEECCCCCEEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIHAGGLQVPVSVIIPAAGSGRRMGGGTAKQFLPLRGEPVLVRTVRRFSECPLVDEIVI
CCCCCCCCCCEEEEEECCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCHHHHEE
AAGDVEATRALVGHMPKVTRIVQGGAERQDSVWAALQAVHSRPRIVAVHDAARPLLTADV
ECCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
LKGVLQAAADHPAQVVAVPVQDTIKQVGPDGVVVATPDRSMLWAVQTPQVFWADVLVRAF
HHHHHHHHCCCCCEEEEECHHHHHHHCCCCCEEEECCCCCEEEEECCCHHHHHHHHHHHH
RQAIADGFLGTDDASLVERIGVPVRVYRGHPGNLKLTTPTDFRLAELLLEEEARQC
HHHHHCCCCCCCHHHHHHHCCCCEEEEECCCCCEEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA