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The map label for this gene is ispF

Identifier: 51894257

GI number: 51894257

Start: 3322506

End: 3322979

Strand: Reverse

Name: ispF

Synonym: STH3122

Alternate gene names: 51894257

Gene position: 3322979-3322506 (Counterclockwise)

Preceding gene: 51894258

Following gene: 51894256

Centisome position: 93.18

GC content: 71.73

Gene sequence:

>474_bases
ATGCTGATCGGCTTCGGTTACGACGTTCACCGGCTGGTCCCCGGGAGGCCGCTGGTCCTGGGCGGCGTGGAGATCCCCAG
CAAGCGGGGCTTGGAGGGGCACTCCGACGCGGACGTGGCCCTGCACGCCCTCATGGACGCCTTGCTCGGGGCCGCCGGGC
TGGGAGACATCGGCACGCACTTTCCGCCGACGGACCCGGCGTGGGCGGGGGCCAACTCCCTGGACCTGCTCCGCCGGGTG
GTGGGTCTGCTGGCCGAGGAGGGTTACCGGCCCGTAAACTGCGACATCACCGTGGTGGCCGAGCGGCCGAAGATCGGGCC
CTACGTGCCGGCGATGCGGGAGCGGATCGGCGCCGTGCTGGGCGTCGAGCCGCGCCGGGTCAGCATCAAGGCCACGACCA
ACGAGCAGATGGGCTTCGTCGGTCGCGAAGAGGGCATCGCCGCCTATGCGGTCGCCCTCATCGCCGGGCGGTGA

Upstream 100 bases:

>100_bases
TGCCCGTGCGGGTCTACCGGGGCCATCCGGGCAACCTCAAGCTGACCACGCCGACGGACTTCCGCCTGGCGGAGCTGCTG
CTGGAGGAGGAGGCGCGACA

Downstream 100 bases:

>100_bases
GGGACCCCGCTTGACATCTTCGGCGCCCCCCCGTAAAATCAGAGCAAACGTAAAGGCAGCGAACGGGAGAAGTACCTGCG
GGAGCCTGTACAGAGAGCCA

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MLIGFGYDVHRLVPGRPLVLGGVEIPSKRGLEGHSDADVALHALMDALLGAAGLGDIGTHFPPTDPAWAGANSLDLLRRV
VGLLAEEGYRPVNCDITVVAERPKIGPYVPAMRERIGAVLGVEPRRVSIKATTNEQMGFVGREEGIAAYAVALIAGR

Sequences:

>Translated_157_residues
MLIGFGYDVHRLVPGRPLVLGGVEIPSKRGLEGHSDADVALHALMDALLGAAGLGDIGTHFPPTDPAWAGANSLDLLRRV
VGLLAEEGYRPVNCDITVVAERPKIGPYVPAMRERIGAVLGVEPRRVSIKATTNEQMGFVGREEGIAAYAVALIAGR
>Mature_157_residues
MLIGFGYDVHRLVPGRPLVLGGVEIPSKRGLEGHSDADVALHALMDALLGAAGLGDIGTHFPPTDPAWAGANSLDLLRRV
VGLLAEEGYRPVNCDITVVAERPKIGPYVPAMRERIGAVLGVEPRRVSIKATTNEQMGFVGREEGIAAYAVALIAGR

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=53.2467532467532, Blast_Score=148, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_SYMTH (Q67JP6)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_076948.1
- ProteinModelPortal:   Q67JP6
- SMR:   Q67JP6
- GeneID:   2979925
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH3122
- NMPDR:   fig|292459.1.peg.2993
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   CLSK2523411
- BioCyc:   STHE292459:STH3122-MONOMER
- BRENDA:   4.6.1.12
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16571; Mature: 16571

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGFGYDVHRLVPGRPLVLGGVEIPSKRGLEGHSDADVALHALMDALLGAAGLGDIGTH
CEEECCCCHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
FPPTDPAWAGANSLDLLRRVVGLLAEEGYRPVNCDITVVAERPKIGPYVPAMRERIGAVL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
GVEPRRVSIKATTNEQMGFVGREEGIAAYAVALIAGR
CCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MLIGFGYDVHRLVPGRPLVLGGVEIPSKRGLEGHSDADVALHALMDALLGAAGLGDIGTH
CEEECCCCHHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
FPPTDPAWAGANSLDLLRRVVGLLAEEGYRPVNCDITVVAERPKIGPYVPAMRERIGAVL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
GVEPRRVSIKATTNEQMGFVGREEGIAAYAVALIAGR
CCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA