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The map label for this gene is liaS [H]

Identifier: 51894232

GI number: 51894232

Start: 3297272

End: 3298378

Strand: Reverse

Name: liaS [H]

Synonym: STH3097

Alternate gene names: 51894232

Gene position: 3298378-3297272 (Counterclockwise)

Preceding gene: 51894240

Following gene: 51894231

Centisome position: 92.49

GC content: 75.16

Gene sequence:

>1107_bases
TTGGATCTACAGGGGGTGTGGCCGGTGCAGGCGGAGTGGGTGTCCCGGGCGGTGGCGGCCCTTCCGGCCGCGGGTTCGGA
GCCGGAGCTGGCTGCCGCCGTGGCCCGGGCAGCCGCGGAGGCGCTGGGCTGGCGCGCCGCGGGGGTGCGGCGCGCCGGCC
GGACGGAGTGGCTGGCCCGGTTTCCCGAAGGCGCCGAGGTGCGCGGCGAAGTCAACCTGCCCCTCGCCGGGGGGCTGGAG
CTGGCCGCGGACGGAGCCGGGGACGCGGAGGGGCTGGCGCTCCTGGGCGCGGTGGCCGGCAAGCTGCTGGAGGCGGCGCA
GGAGCGGGCGCGCCTCGCCCAGCGGGCCGAGCGGGCGGAGCGGCGGGCGCGGGAGGCCGTGGAGCGGTACCGCAGCCAGA
CCGAGCGGCTGGCCGCGCTGCGGGGTGAGCTCAGCGAGGCGCAGCAGCGCCAGCTACTCCTGAGCGAGCGGAACCGGATC
GCCCAGGACCTGCACGACCGGGCCGCCCAGACCGCCTACCTGGTGGCGCTGAAGCTGGACTGGCTGCTCGGCGACCTGCC
GCCTGCCGACCCGCTGCGGGAGGAGCTGGAGCGGCTCCGGGGGCTGGCAGCGGAGGCGGCGGCGCAGACCCGGGAGGCGA
TCTTCGCCCTGCGGGCCCCGGAGCTCAGCGAGGGCGGGCTGCACGGCGGGCTGCGCCGGCTCCTGCGGGAGGCGGAGCGC
GACGGGTTCACCACGCGCCTCACCGTCGCAGGCAGGCCCCGGGCCCTGCCGGCCGAGGTGGAGGACGCCCTCTTCAAGGT
GGGCCAGGAGGCCCTGACCAACGCCCGCAAGCACAGCGGGGGCAGCGCCGTCATGGTGGCGCTCCGCTACGAGCCGGCAG
CGGTGACCCTGGTCGTGCAGGATAATGGCGCGGGGATAAAGAATCGCACTGATATTGAGAAACCAGGAAGGTTTGGTGTT
AGAGGTATGCGTGAGCGGGTGGCCCGGCTGGGGGGACGGCTGGAGCTGGTGGACGGCGACGAGGGCGGTCTCCTGGTCAG
GGCCTCGGTGCCGCTGAAGGGGGTGGACACGCATGCCCATCCGTGTCCTGATCGTGGACGACCATGA

Upstream 100 bases:

>100_bases
GAACGAGCCGCCCGGCGGCTCTTCACCTAGGCGTTCGGTCCTGTTCGCTTCCCCGTTCGGTCGCTGTTCGTAAGTTGTTA
TACCCGAGCGGGCTGCGTAC

Downstream 100 bases:

>100_bases
GCTGCTGAGGGAGGGGCTGAAGTCGCTGCTCTCCCGGGAGGAGGACATGGAGGTGGTGGGGGAGGCCGCGGGGGGACGGG
AGGCCGTCGCCCTCTGCCGG

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MDLQGVWPVQAEWVSRAVAALPAAGSEPELAAAVARAAAEALGWRAAGVRRAGRTEWLARFPEGAEVRGEVNLPLAGGLE
LAADGAGDAEGLALLGAVAGKLLEAAQERARLAQRAERAERRAREAVERYRSQTERLAALRGELSEAQQRQLLLSERNRI
AQDLHDRAAQTAYLVALKLDWLLGDLPPADPLREELERLRGLAAEAAAQTREAIFALRAPELSEGGLHGGLRRLLREAER
DGFTTRLTVAGRPRALPAEVEDALFKVGQEALTNARKHSGGSAVMVALRYEPAAVTLVVQDNGAGIKNRTDIEKPGRFGV
RGMRERVARLGGRLELVDGDEGGLLVRASVPLKGVDTHAHPCPDRGRP

Sequences:

>Translated_368_residues
MDLQGVWPVQAEWVSRAVAALPAAGSEPELAAAVARAAAEALGWRAAGVRRAGRTEWLARFPEGAEVRGEVNLPLAGGLE
LAADGAGDAEGLALLGAVAGKLLEAAQERARLAQRAERAERRAREAVERYRSQTERLAALRGELSEAQQRQLLLSERNRI
AQDLHDRAAQTAYLVALKLDWLLGDLPPADPLREELERLRGLAAEAAAQTREAIFALRAPELSEGGLHGGLRRLLREAER
DGFTTRLTVAGRPRALPAEVEDALFKVGQEALTNARKHSGGSAVMVALRYEPAAVTLVVQDNGAGIKNRTDIEKPGRFGV
RGMRERVARLGGRLELVDGDEGGLLVRASVPLKGVDTHAHPCPDRGRP
>Mature_368_residues
MDLQGVWPVQAEWVSRAVAALPAAGSEPELAAAVARAAAEALGWRAAGVRRAGRTEWLARFPEGAEVRGEVNLPLAGGLE
LAADGAGDAEGLALLGAVAGKLLEAAQERARLAQRAERAERRAREAVERYRSQTERLAALRGELSEAQQRQLLLSERNRI
AQDLHDRAAQTAYLVALKLDWLLGDLPPADPLREELERLRGLAAEAAAQTREAIFALRAPELSEGGLHGGLRRLLREAER
DGFTTRLTVAGRPRALPAEVEDALFKVGQEALTNARKHSGGSAVMVALRYEPAAVTLVVQDNGAGIKNRTDIEKPGRFGV
RGMRERVARLGGRLELVDGDEGGLLVRASVPLKGVDTHAHPCPDRGRP

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=222, Percent_Identity=31.981981981982, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788812, Length=206, Percent_Identity=31.0679611650485, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 39516; Mature: 39516

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLQGVWPVQAEWVSRAVAALPAAGSEPELAAAVARAAAEALGWRAAGVRRAGRTEWLAR
CCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
FPEGAEVRGEVNLPLAGGLELAADGAGDAEGLALLGAVAGKLLEAAQERARLAQRAERAE
CCCCCCCCCEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRAREAVERYRSQTERLAALRGELSEAQQRQLLLSERNRIAQDLHDRAAQTAYLVALKLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLLGDLPPADPLREELERLRGLAAEAAAQTREAIFALRAPELSEGGLHGGLRRLLREAER
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
DGFTTRLTVAGRPRALPAEVEDALFKVGQEALTNARKHSGGSAVMVALRYEPAAVTLVVQ
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEEE
DNGAGIKNRTDIEKPGRFGVRGMRERVARLGGRLELVDGDEGGLLVRASVPLKGVDTHAH
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCEEEEECCCCCCCCCCCC
PCPDRGRP
CCCCCCCC
>Mature Secondary Structure
MDLQGVWPVQAEWVSRAVAALPAAGSEPELAAAVARAAAEALGWRAAGVRRAGRTEWLAR
CCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
FPEGAEVRGEVNLPLAGGLELAADGAGDAEGLALLGAVAGKLLEAAQERARLAQRAERAE
CCCCCCCCCEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRAREAVERYRSQTERLAALRGELSEAQQRQLLLSERNRIAQDLHDRAAQTAYLVALKLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLLGDLPPADPLREELERLRGLAAEAAAQTREAIFALRAPELSEGGLHGGLRRLLREAER
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
DGFTTRLTVAGRPRALPAEVEDALFKVGQEALTNARKHSGGSAVMVALRYEPAAVTLVVQ
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEEE
DNGAGIKNRTDIEKPGRFGVRGMRERVARLGGRLELVDGDEGGLLVRASVPLKGVDTHAH
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCEEEEECCCCCCCCCCCC
PCPDRGRP
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]