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The map label for this gene is leuB

Identifier: 51894171

GI number: 51894171

Start: 3251667

End: 3252773

Strand: Reverse

Name: leuB

Synonym: STH3035

Alternate gene names: 51894171

Gene position: 3252773-3251667 (Counterclockwise)

Preceding gene: 51894172

Following gene: 51894166

Centisome position: 91.21

GC content: 70.37

Gene sequence:

>1107_bases
TTGGCTCAGCCTACCTACCACATTGCCTGCCTCCCGGGCGACGGGATCGGTCCCGAGGTCACCCGGGGGGCAGTGACCGT
GCTGCAGGCAGCCGCGGCTGCCTACGGTTTCCGCCTGGAGTTTTCGGAGTACCTGGTGGGCGGAGCCGCTTACGACGCTG
TGGGAACCCCCTTTCCGGACGAGACGCGGGATGCGTGTGACCGCGCGGATGCCATCCTCTTCGGCGCCGTGGGCGGTCCC
CGCTACGAGGGGCTGCCGTGGGACCTGCGCCCGGAGGCCGGCCTGCTGGCCATCCGGAAGCGCTACGACCTGTACGCGAA
CCTGCGTCCCATCCTGCTCTACCCCCCGCTGAAGGACGCCTCGCCGCTGAAGAACGAGATCATCGGCGGCGGCGTCGATT
TCATCATCGTGCGGGAGCTGGTGGGCGGCATCTACTTTGGCGAGCCTCGGGGCATCGAGACCCTGCCCGACGGCACTCGC
CGGGGCGTGAACACCGAGGTGTACACCGACGCCGAGATCGCCCGCATCGCGCGCATGGGCTTCGAAATCGCCCGGGGGCG
CAGGCGTAGGCTGACCTCGGTGGACAAGGGCAACGTGATGGAGGCCGGCAAGCTGTGGCGGACCGTGGTCGACGAGGTGG
CCCGGGAGTTCCCCGACGTCGAGGTGGAGCATCTCCTGGCGGACAACGCGGCGATGCAGATCCTGCGCCGCCCCGGCGAT
TTCGACGTAATGCTGGCCTCCAACCTGTTCGGGGACTTTCTCTCCGACGAGGCGGCCATGCTGACCGGCTCCATCGGCCT
GCTGCCCTCGGCCTCGCTCGGGGCGGCGCGCAACCGATTCGGCCTGCCCAAGGGCTTCTATGAACCCATCCACGGCTCCG
CGCCGGACATCGCCGGCCAGGACCGGGCCAACCCCCTGGCCGCCATCCTCTGCGGCGCCATGCTCCTGCGTCACTCGCTG
GGCCGGGAGGACGCGGCCCGGGCGGTGGAACAGGCGGTGGCTGCGGTCCTGGAAGAGGGTCTGCGCACCGCCGACATCAC
CGTGCCCGGCACTGCGGTCCTCGGCACGGCGGCGATGGCCCGCGCGGTCGCCGACCGGGTCCACTGA

Upstream 100 bases:

>100_bases
TGCGCATGGAGAGGTTCCTCCAGCGGGCGGGGGAAAAGCGGTTGCCTTAAATGCTTTAACGTGCTAAACTTCCTCCAAAC
CCGATGGAGGTGTTCCAGAG

Downstream 100 bases:

>100_bases
CGGGTACGGGGGCTGTCTCCAGGGTCATCGGGGATGACCTCGGGACAGCCCCCGGCGCTTACTCGTCGTCGTCGTCCTTC
TCCTTGCCGCCGGCGTTCAC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MAQPTYHIACLPGDGIGPEVTRGAVTVLQAAAAAYGFRLEFSEYLVGGAAYDAVGTPFPDETRDACDRADAILFGAVGGP
RYEGLPWDLRPEAGLLAIRKRYDLYANLRPILLYPPLKDASPLKNEIIGGGVDFIIVRELVGGIYFGEPRGIETLPDGTR
RGVNTEVYTDAEIARIARMGFEIARGRRRRLTSVDKGNVMEAGKLWRTVVDEVAREFPDVEVEHLLADNAAMQILRRPGD
FDVMLASNLFGDFLSDEAAMLTGSIGLLPSASLGAARNRFGLPKGFYEPIHGSAPDIAGQDRANPLAAILCGAMLLRHSL
GREDAARAVEQAVAAVLEEGLRTADITVPGTAVLGTAAMARAVADRVH

Sequences:

>Translated_368_residues
MAQPTYHIACLPGDGIGPEVTRGAVTVLQAAAAAYGFRLEFSEYLVGGAAYDAVGTPFPDETRDACDRADAILFGAVGGP
RYEGLPWDLRPEAGLLAIRKRYDLYANLRPILLYPPLKDASPLKNEIIGGGVDFIIVRELVGGIYFGEPRGIETLPDGTR
RGVNTEVYTDAEIARIARMGFEIARGRRRRLTSVDKGNVMEAGKLWRTVVDEVAREFPDVEVEHLLADNAAMQILRRPGD
FDVMLASNLFGDFLSDEAAMLTGSIGLLPSASLGAARNRFGLPKGFYEPIHGSAPDIAGQDRANPLAAILCGAMLLRHSL
GREDAARAVEQAVAAVLEEGLRTADITVPGTAVLGTAAMARAVADRVH
>Mature_367_residues
AQPTYHIACLPGDGIGPEVTRGAVTVLQAAAAAYGFRLEFSEYLVGGAAYDAVGTPFPDETRDACDRADAILFGAVGGPR
YEGLPWDLRPEAGLLAIRKRYDLYANLRPILLYPPLKDASPLKNEIIGGGVDFIIVRELVGGIYFGEPRGIETLPDGTRR
GVNTEVYTDAEIARIARMGFEIARGRRRRLTSVDKGNVMEAGKLWRTVVDEVAREFPDVEVEHLLADNAAMQILRRPGDF
DVMLASNLFGDFLSDEAAMLTGSIGLLPSASLGAARNRFGLPKGFYEPIHGSAPDIAGQDRANPLAAILCGAMLLRHSLG
REDAARAVEQAVAAVLEEGLRTADITVPGTAVLGTAAMARAVADRVH

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=336, Percent_Identity=32.7380952380952, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI28178816, Length=321, Percent_Identity=27.7258566978193, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI28178821, Length=321, Percent_Identity=27.7258566978193, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI4758582, Length=359, Percent_Identity=28.6908077994429, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI28178838, Length=345, Percent_Identity=28.1159420289855, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI28178819, Length=155, Percent_Identity=32.9032258064516, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI87081683, Length=364, Percent_Identity=52.1978021978022, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=37.3595505617978, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1787381, Length=407, Percent_Identity=26.7813267813268, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71986051, Length=367, Percent_Identity=30.2452316076294, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17550882, Length=351, Percent_Identity=30.4843304843305, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17505779, Length=345, Percent_Identity=29.2753623188406, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25144293, Length=354, Percent_Identity=28.8135593220339, Blast_Score=115, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=352, Percent_Identity=43.75, Blast_Score=271, Evalue=9e-74,
Organism=Saccharomyces cerevisiae, GI6322097, Length=384, Percent_Identity=32.8125, Blast_Score=156, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6324709, Length=371, Percent_Identity=30.7277628032345, Blast_Score=136, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324291, Length=348, Percent_Identity=29.0229885057471, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24643268, Length=346, Percent_Identity=30.9248554913295, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24643270, Length=346, Percent_Identity=30.9248554913295, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24661184, Length=349, Percent_Identity=29.512893982808, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI281362242, Length=341, Percent_Identity=27.8592375366569, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24648872, Length=341, Percent_Identity=27.8592375366569, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI161078633, Length=345, Percent_Identity=27.536231884058, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24650122, Length=345, Percent_Identity=27.536231884058, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI161078637, Length=345, Percent_Identity=27.536231884058, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078635, Length=345, Percent_Identity=27.536231884058, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078639, Length=342, Percent_Identity=27.7777777777778, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI20130355, Length=222, Percent_Identity=30.1801801801802, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_SYMTH (Q67JY2)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_076862.1
- ProteinModelPortal:   Q67JY2
- SMR:   Q67JY2
- GeneID:   2981045
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH3035
- NMPDR:   fig|292459.1.peg.2907
- HOGENOM:   HBG518924
- OMA:   NTMIYSE
- BioCyc:   STHE292459:STH3035-MONOMER
- BRENDA:   1.1.1.85
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 39411; Mature: 39280

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 99-99 BINDING 109-109 BINDING 138-138 BINDING 228-228

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQPTYHIACLPGDGIGPEVTRGAVTVLQAAAAAYGFRLEFSEYLVGGAAYDAVGTPFPD
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEEHHHHHHCCHHHHCCCCCCCC
ETRDACDRADAILFGAVGGPRYEGLPWDLRPEAGLLAIRKRYDLYANLRPILLYPPLKDA
HHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCC
SPLKNEIIGGGVDFIIVRELVGGIYFGEPRGIETLPDGTRRGVNTEVYTDAEIARIARMG
CHHHHHHCCCCHHHHHHHHHHCCEECCCCCCCCCCCCCHHCCCCCEEECHHHHHHHHHHH
FEIARGRRRRLTSVDKGNVMEAGKLWRTVVDEVAREFPDVEVEHLLADNAAMQILRRPGD
HHHHHCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
FDVMLASNLFGDFLSDEAAMLTGSIGLLPSASLGAARNRFGLPKGFYEPIHGSAPDIAGQ
CEEEEHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCC
DRANPLAAILCGAMLLRHSLGREDAARAVEQAVAAVLEEGLRTADITVPGTAVLGTAAMA
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHH
RAVADRVH
HHHHHHCC
>Mature Secondary Structure 
AQPTYHIACLPGDGIGPEVTRGAVTVLQAAAAAYGFRLEFSEYLVGGAAYDAVGTPFPD
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEEEHHHHHHCCHHHHCCCCCCCC
ETRDACDRADAILFGAVGGPRYEGLPWDLRPEAGLLAIRKRYDLYANLRPILLYPPLKDA
HHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCC
SPLKNEIIGGGVDFIIVRELVGGIYFGEPRGIETLPDGTRRGVNTEVYTDAEIARIARMG
CHHHHHHCCCCHHHHHHHHHHCCEECCCCCCCCCCCCCHHCCCCCEEECHHHHHHHHHHH
FEIARGRRRRLTSVDKGNVMEAGKLWRTVVDEVAREFPDVEVEHLLADNAAMQILRRPGD
HHHHHCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
FDVMLASNLFGDFLSDEAAMLTGSIGLLPSASLGAARNRFGLPKGFYEPIHGSAPDIAGQ
CEEEEHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCC
DRANPLAAILCGAMLLRHSLGREDAARAVEQAVAAVLEEGLRTADITVPGTAVLGTAAMA
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHH
RAVADRVH
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA