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The map label for this gene is argG

Identifier: 51894010

GI number: 51894010

Start: 3097033

End: 3098238

Strand: Reverse

Name: argG

Synonym: STH2874

Alternate gene names: 51894010

Gene position: 3098238-3097033 (Counterclockwise)

Preceding gene: 51894011

Following gene: 51894009

Centisome position: 86.88

GC content: 65.59

Gene sequence:

>1206_bases
ATGGCGAAGAAGAAGTGCGTGCTGGCCTACTCAGGCGGGCTGGATACCTCGGTGGCGATCCACTGGATCAAGGAGAACTA
CGACGCCGATGTGGTCGCGCTGGCGGTGGACGTGGGCGCCAACGACAAGGACCTGGAGTTCATCCGGCAGAAGGCGCTTT
CCATCGGCGCCGTGAAGAGCTACGTCTACGATGCCAGGAAAGAGTTCGCCTACGACTTCATCCTGCCGACGCTCCAGGCC
AACGCCTGTTACGAGTCCAAGTACTACCTCTCGGCGGCGCTTTCCCGGCCGCTGATCGCGAAGCTGCTGGTCCAGATCGC
CGAGGAGGAAGGGGCCGACTTCGTCGCCCACGGCTGCACGGGCAAGGGCAATGACCAGGTGCGGTTCGAGGTCTCCGTGG
CCGCCCTCAACCCCAACCTGCAGGTGCTCGCCCCGGTGCGGGAGTGGGGATGGAGCCGGGAGGAGGAGATCGACTACGCC
CAGAAACATGGCATCCCGGTCCCGGTGGGCAAGGAGAACCCGTTCTCCATCGACGTGAACCTGTGGGGCCGCTCCTGCGA
GGCCGGCGTCCTGGAGGACCCGTGGGCGGAGGCGCCGGAGGAGGCCTTCGAGTGGACCGTGAATCCGAAGGATGCCCCCG
ACGAGCCCGAGTACGTGGAGATCGGCTTCGAGCAGGGCGTGCCGGTCTCGTTGAACGGCGAGGCGCTGGACCTGGTGACC
CTGATCGAGCGGCTGCACGCCATCGCCGGCCGCCACGGGGTGGGCCGCATCGACCACGTGGAGAACCGACTGGTCGGCAT
CAAGTCCCGGGAGGTGTACGAGATGCCGGCGGCGGCGGTGCTCACCCTGGCGCACAAGGAGCTGGAGACCATCACCCTGC
CCCGGGAGCTGCACCACTTCAAGCTGGGCCTGGAGCAGAAGTACGCCGAACTGGTCTACAACGGCCTCTGGTTCCACCCG
CTGAAGGAGGCCCTGGACGCTTTCATCAAGAAGTCCCAGGAGACGGTGACCGGCACGGTGCGGGTGAGACTCTTCAAGGG
CTCGGCCTTCTGCGTGGGCCGGACTTCGCCCTACACGCTCTACTCCTACGACCTGGCCACCTACGACAAGGCGGACAAGT
TCGACCACAAGGCGGCCAAGGGGTTCATCGACATCTTCGGCCTGCCCACCAAGGTCTACGCCGCCGTGCAGAAGAGCAAG
GCCTGA

Upstream 100 bases:

>100_bases
TCTAAATCTATTCACCCCTATTGCATACTTATTCACCTAGGAGTATAATCATGCACCAAGAGGCCCGGGGCGCCGGGCGA
GGACGAGGAGGCTCCGATCG

Downstream 100 bases:

>100_bases
GACGCATACGGATGACTGGCGACCGCCCGGACATCGGACCCGGGCGGTCGGTTGTCTGCGGAGGACCCGCATGCCTGCCG
GGGGCAGGAAAGCCGCTTCC

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MAKKKCVLAYSGGLDTSVAIHWIKENYDADVVALAVDVGANDKDLEFIRQKALSIGAVKSYVYDARKEFAYDFILPTLQA
NACYESKYYLSAALSRPLIAKLLVQIAEEEGADFVAHGCTGKGNDQVRFEVSVAALNPNLQVLAPVREWGWSREEEIDYA
QKHGIPVPVGKENPFSIDVNLWGRSCEAGVLEDPWAEAPEEAFEWTVNPKDAPDEPEYVEIGFEQGVPVSLNGEALDLVT
LIERLHAIAGRHGVGRIDHVENRLVGIKSREVYEMPAAAVLTLAHKELETITLPRELHHFKLGLEQKYAELVYNGLWFHP
LKEALDAFIKKSQETVTGTVRVRLFKGSAFCVGRTSPYTLYSYDLATYDKADKFDHKAAKGFIDIFGLPTKVYAAVQKSK
A

Sequences:

>Translated_401_residues
MAKKKCVLAYSGGLDTSVAIHWIKENYDADVVALAVDVGANDKDLEFIRQKALSIGAVKSYVYDARKEFAYDFILPTLQA
NACYESKYYLSAALSRPLIAKLLVQIAEEEGADFVAHGCTGKGNDQVRFEVSVAALNPNLQVLAPVREWGWSREEEIDYA
QKHGIPVPVGKENPFSIDVNLWGRSCEAGVLEDPWAEAPEEAFEWTVNPKDAPDEPEYVEIGFEQGVPVSLNGEALDLVT
LIERLHAIAGRHGVGRIDHVENRLVGIKSREVYEMPAAAVLTLAHKELETITLPRELHHFKLGLEQKYAELVYNGLWFHP
LKEALDAFIKKSQETVTGTVRVRLFKGSAFCVGRTSPYTLYSYDLATYDKADKFDHKAAKGFIDIFGLPTKVYAAVQKSK
A
>Mature_400_residues
AKKKCVLAYSGGLDTSVAIHWIKENYDADVVALAVDVGANDKDLEFIRQKALSIGAVKSYVYDARKEFAYDFILPTLQAN
ACYESKYYLSAALSRPLIAKLLVQIAEEEGADFVAHGCTGKGNDQVRFEVSVAALNPNLQVLAPVREWGWSREEEIDYAQ
KHGIPVPVGKENPFSIDVNLWGRSCEAGVLEDPWAEAPEEAFEWTVNPKDAPDEPEYVEIGFEQGVPVSLNGEALDLVTL
IERLHAIAGRHGVGRIDHVENRLVGIKSREVYEMPAAAVLTLAHKELETITLPRELHHFKLGLEQKYAELVYNGLWFHPL
KEALDAFIKKSQETVTGTVRVRLFKGSAFCVGRTSPYTLYSYDLATYDKADKFDHKAAKGFIDIFGLPTKVYAAVQKSKA

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=407, Percent_Identity=44.2260442260442, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI16950633, Length=407, Percent_Identity=44.2260442260442, Blast_Score=320, Evalue=2e-87,
Organism=Escherichia coli, GI1789563, Length=400, Percent_Identity=29.25, Blast_Score=134, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324514, Length=417, Percent_Identity=40.2877697841727, Blast_Score=291, Evalue=1e-79,
Organism=Drosophila melanogaster, GI21358151, Length=403, Percent_Identity=39.2059553349876, Blast_Score=270, Evalue=1e-72,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_SYMTH (Q67KE1)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_076701.1
- ProteinModelPortal:   Q67KE1
- SMR:   Q67KE1
- GeneID:   2982529
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH2874
- NMPDR:   fig|292459.1.peg.2749
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- ProtClustDB:   CLSK2765221
- BioCyc:   STHE292459:STH2874-MONOMER
- BRENDA:   6.3.4.5
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44594; Mature: 44463

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 88-88 BINDING 118-118 BINDING 120-120 BINDING 124-124 BINDING 124-124 BINDING 125-125 BINDING 128-128 BINDING 176-176 BINDING 185-185 BINDING 261-261 BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKKCVLAYSGGLDTSVAIHWIKENYDADVVALAVDVGANDKDLEFIRQKALSIGAVKS
CCCCCEEEEECCCCCCEEEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
YVYDARKEFAYDFILPTLQANACYESKYYLSAALSRPLIAKLLVQIAEEEGADFVAHGCT
HHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCC
GKGNDQVRFEVSVAALNPNLQVLAPVREWGWSREEEIDYAQKHGIPVPVGKENPFSIDVN
CCCCCEEEEEEEEEEECCCCEEEEEHHHCCCCCHHHCCHHHHCCCCCCCCCCCCEEEEEE
LWGRSCEAGVLEDPWAEAPEEAFEWTVNPKDAPDEPEYVEIGFEQGVPVSLNGEALDLVT
ECCCCCCCCCCCCCCCCCCHHHHEEECCCCCCCCCCCEEEEECCCCCCEEECCCHHHHHH
LIERLHAIAGRHGVGRIDHVENRLVGIKSREVYEMPAAAVLTLAHKELETITLPRELHHF
HHHHHHHHHCCCCCCCHHHHHHHEECCCCCHHHHCCHHHHHHHHHHHHHEEECCHHHHHH
KLGLEQKYAELVYNGLWFHPLKEALDAFIKKSQETVTGTVRVRLFKGSAFCVGRTSPYTL
HCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCEEEEECCCCCEE
YSYDLATYDKADKFDHKAAKGFIDIFGLPTKVYAAVQKSKA
EEECCCCCCCCHHHHHHHHCCEEEEECCCHHHHHHHHHCCC
>Mature Secondary Structure 
AKKKCVLAYSGGLDTSVAIHWIKENYDADVVALAVDVGANDKDLEFIRQKALSIGAVKS
CCCCEEEEECCCCCCEEEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
YVYDARKEFAYDFILPTLQANACYESKYYLSAALSRPLIAKLLVQIAEEEGADFVAHGCT
HHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCC
GKGNDQVRFEVSVAALNPNLQVLAPVREWGWSREEEIDYAQKHGIPVPVGKENPFSIDVN
CCCCCEEEEEEEEEEECCCCEEEEEHHHCCCCCHHHCCHHHHCCCCCCCCCCCCEEEEEE
LWGRSCEAGVLEDPWAEAPEEAFEWTVNPKDAPDEPEYVEIGFEQGVPVSLNGEALDLVT
ECCCCCCCCCCCCCCCCCCHHHHEEECCCCCCCCCCCEEEEECCCCCCEEECCCHHHHHH
LIERLHAIAGRHGVGRIDHVENRLVGIKSREVYEMPAAAVLTLAHKELETITLPRELHHF
HHHHHHHHHCCCCCCCHHHHHHHEECCCCCHHHHCCHHHHHHHHHHHHHEEECCHHHHHH
KLGLEQKYAELVYNGLWFHPLKEALDAFIKKSQETVTGTVRVRLFKGSAFCVGRTSPYTL
HCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCEEEEECCCCCEE
YSYDLATYDKADKFDHKAAKGFIDIFGLPTKVYAAVQKSKA
EEECCCCCCCCHHHHHHHHCCEEEEECCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA