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The map label for this gene is purD [H]

Identifier: 51893987

GI number: 51893987

Start: 3070325

End: 3071593

Strand: Reverse

Name: purD [H]

Synonym: STH2849

Alternate gene names: 51893987

Gene position: 3071593-3070325 (Counterclockwise)

Preceding gene: 51893988

Following gene: 51893986

Centisome position: 86.13

GC content: 70.76

Gene sequence:

>1269_bases
ATGAAGGTCCTGATCGTCGGCGGCGGAGGACGCGAGCACGCCCTCGCCTGGAAGGCGATCCAGAGCAGGCACATCGACGA
GCTGCACGCGGCGCCGGGGAACCCCGGCATCGGCCGGTTCGCGTGGTGCCATCCCGACGTGGAGGCCACCGATGTCGCCG
GGCAGGTGGACCTGGCCCGCCGGCTGGGGATCGACCTGGTCATCGTGGGCCCCGAGGCTCCGCTGGCGGCGGGGCTGGTG
GATGCGCTGACCGCCGCAGGCATCCCGGCTTTCGGCCCCACGGCCGCGGCGGCTCGCATCGAGGCCTCCAAAGCCTACGC
CAAGGAGGTCATGACCGCCGCCGGCGTGCCGACCGCGGCATACGCCGACTTCACGGATTACCAGCAGGCACTGGCGTACA
TCGAGGCCCGCCCGGGTCCCGTCGTCATCAAGGCGGACGGGCTCGCGGCGGGCAAGGGTGTGCAGGTCTGTGCTGACCTG
GACGAGGCACGTGCTGCGCTGCGGGCGGCGATGGTGGAGGGCGCCTTCGACCAGGCCGGAAGCCGGGTCGTCATCGAGGA
GGTCCTGGAGGGACAGGAGGTCTCCGTCCTGGCCTTCAGCGACGGGCGCACGGTGAAGCAGATGGTCGCCGCCCAGGACC
ACAAGCGGGCGGGCGAGGGTGACACCGGCCCCAACACGGGCGGGATGGGCGCCTACGCACCGGTGCCGGCCTACACCGCA
GACATGGCCGAGGCGGTGCAGCGGCGCATCCTGGAACCGACCATCGCCGAGCTTGCCCGGCGGGGCACGCCGTTCGTGGG
CTGCCTCTTCGCCGGCCTCATGCTGACCGCCGACGGTCCGAAGGTGATCGAGTTCAACGCACGCTTCGGCGACCCGGAAA
CCCAGGCCGTGCTCCCGCTTCTGGAGAACGACCTGCTCGAGGTGATCCAGGCCTGCCTGGAGGCGCGGCTGCACGAGGTG
GATCTCCGCTTCCGCCCCGGCGCCGCCGTCAACGTGGTGCTCGCCTCCGCCGGCTATCCCGGGAAGTACGTCAAGGGCCT
GCCCATCAGCGGGCTGGTGGAGGCCGACCAGGTCGGCGTCACCGTGTTCCACGCGGGCACGGCCTTCAACGAGGACGGTC
AGGTGGTCACCGCAGGTGGCCGGGTGCTCGGGGTGATGGCGACGGGCCCCACTCTGCGGGATGCGCTGGCAATGGCGTAC
GCCGGGGTGGATCAGATTCAGTTCGAAGGCAAGACCTATCGACGCGATATTGGCTGGCGTGCGCTCTGA

Upstream 100 bases:

>100_bases
CGGGCGCGGTGGTCAGCCTTTTGGGGTAGAAATAATGGAAAAATGCTGGGTATGCCAGGAACGGCGAGGTCGGCAGGGAA
TAAGTAAGGAGTGTGAGAAA

Downstream 100 bases:

>100_bases
GCGGCCGTCCCAACGGTCATCCTATGGGCTACGGGGAAGCTGGCTCCGAGCCCTGCCGGCGTTAAGTGACGGATGAAAGG
TCGATGATGCGTGTCTCTGG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKVLIVGGGGREHALAWKAIQSRHIDELHAAPGNPGIGRFAWCHPDVEATDVAGQVDLARRLGIDLVIVGPEAPLAAGLV
DALTAAGIPAFGPTAAAARIEASKAYAKEVMTAAGVPTAAYADFTDYQQALAYIEARPGPVVIKADGLAAGKGVQVCADL
DEARAALRAAMVEGAFDQAGSRVVIEEVLEGQEVSVLAFSDGRTVKQMVAAQDHKRAGEGDTGPNTGGMGAYAPVPAYTA
DMAEAVQRRILEPTIAELARRGTPFVGCLFAGLMLTADGPKVIEFNARFGDPETQAVLPLLENDLLEVIQACLEARLHEV
DLRFRPGAAVNVVLASAGYPGKYVKGLPISGLVEADQVGVTVFHAGTAFNEDGQVVTAGGRVLGVMATGPTLRDALAMAY
AGVDQIQFEGKTYRRDIGWRAL

Sequences:

>Translated_422_residues
MKVLIVGGGGREHALAWKAIQSRHIDELHAAPGNPGIGRFAWCHPDVEATDVAGQVDLARRLGIDLVIVGPEAPLAAGLV
DALTAAGIPAFGPTAAAARIEASKAYAKEVMTAAGVPTAAYADFTDYQQALAYIEARPGPVVIKADGLAAGKGVQVCADL
DEARAALRAAMVEGAFDQAGSRVVIEEVLEGQEVSVLAFSDGRTVKQMVAAQDHKRAGEGDTGPNTGGMGAYAPVPAYTA
DMAEAVQRRILEPTIAELARRGTPFVGCLFAGLMLTADGPKVIEFNARFGDPETQAVLPLLENDLLEVIQACLEARLHEV
DLRFRPGAAVNVVLASAGYPGKYVKGLPISGLVEADQVGVTVFHAGTAFNEDGQVVTAGGRVLGVMATGPTLRDALAMAY
AGVDQIQFEGKTYRRDIGWRAL
>Mature_422_residues
MKVLIVGGGGREHALAWKAIQSRHIDELHAAPGNPGIGRFAWCHPDVEATDVAGQVDLARRLGIDLVIVGPEAPLAAGLV
DALTAAGIPAFGPTAAAARIEASKAYAKEVMTAAGVPTAAYADFTDYQQALAYIEARPGPVVIKADGLAAGKGVQVCADL
DEARAALRAAMVEGAFDQAGSRVVIEEVLEGQEVSVLAFSDGRTVKQMVAAQDHKRAGEGDTGPNTGGMGAYAPVPAYTA
DMAEAVQRRILEPTIAELARRGTPFVGCLFAGLMLTADGPKVIEFNARFGDPETQAVLPLLENDLLEVIQACLEARLHEV
DLRFRPGAAVNVVLASAGYPGKYVKGLPISGLVEADQVGVTVFHAGTAFNEDGQVVTAGGRVLGVMATGPTLRDALAMAY
AGVDQIQFEGKTYRRDIGWRAL

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=428, Percent_Identity=49.0654205607477, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI209869995, Length=428, Percent_Identity=49.0654205607477, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI209869993, Length=428, Percent_Identity=49.0654205607477, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI28416899, Length=428, Percent_Identity=49.0654205607477, Blast_Score=395, Evalue=1e-110,
Organism=Escherichia coli, GI1790438, Length=424, Percent_Identity=48.8207547169811, Blast_Score=385, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17567511, Length=426, Percent_Identity=42.4882629107981, Blast_Score=303, Evalue=1e-82,
Organism=Saccharomyces cerevisiae, GI6321203, Length=443, Percent_Identity=41.3092550790068, Blast_Score=320, Evalue=3e-88,
Organism=Drosophila melanogaster, GI62471619, Length=429, Percent_Identity=47.7855477855478, Blast_Score=355, Evalue=5e-98,
Organism=Drosophila melanogaster, GI24582400, Length=429, Percent_Identity=47.7855477855478, Blast_Score=354, Evalue=5e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44015; Mature: 44015

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLIVGGGGREHALAWKAIQSRHIDELHAAPGNPGIGRFAWCHPDVEATDVAGQVDLAR
CEEEEEECCCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEECCCCCHHHCCCHHHHHH
RLGIDLVIVGPEAPLAAGLVDALTAAGIPAFGPTAAAARIEASKAYAKEVMTAAGVPTAA
HCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHH
YADFTDYQQALAYIEARPGPVVIKADGLAAGKGVQVCADLDEARAALRAAMVEGAFDQAG
HCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCC
SRVVIEEVLEGQEVSVLAFSDGRTVKQMVAAQDHKRAGEGDTGPNTGGMGAYAPVPAYTA
CEEHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
DMAEAVQRRILEPTIAELARRGTPFVGCLFAGLMLTADGPKVIEFNARFGDPETQAVLPL
HHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHEEECCCCEEEEEECCCCCCHHHHHHHH
LENDLLEVIQACLEARLHEVDLRFRPGAAVNVVLASAGYPGKYVKGLPISGLVEADQVGV
HHHHHHHHHHHHHHHHHHHCCEEECCCCEEEEEEEECCCCCCCCCCCCCCCCEECCCCCE
TVFHAGTAFNEDGQVVTAGGRVLGVMATGPTLRDALAMAYAGVDQIQFEGKTYRRDIGWR
EEEECCCEECCCCCEEEECCEEEEEEECCCHHHHHHHHHHCCCHHEEECCCHHHHCCCCC
AL
CC
>Mature Secondary Structure
MKVLIVGGGGREHALAWKAIQSRHIDELHAAPGNPGIGRFAWCHPDVEATDVAGQVDLAR
CEEEEEECCCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEECCCCCHHHCCCHHHHHH
RLGIDLVIVGPEAPLAAGLVDALTAAGIPAFGPTAAAARIEASKAYAKEVMTAAGVPTAA
HCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHH
YADFTDYQQALAYIEARPGPVVIKADGLAAGKGVQVCADLDEARAALRAAMVEGAFDQAG
HCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCC
SRVVIEEVLEGQEVSVLAFSDGRTVKQMVAAQDHKRAGEGDTGPNTGGMGAYAPVPAYTA
CEEHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
DMAEAVQRRILEPTIAELARRGTPFVGCLFAGLMLTADGPKVIEFNARFGDPETQAVLPL
HHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHEEECCCCEEEEEECCCCCCHHHHHHHH
LENDLLEVIQACLEARLHEVDLRFRPGAAVNVVLASAGYPGKYVKGLPISGLVEADQVGV
HHHHHHHHHHHHHHHHHHHCCEEECCCCEEEEEEEECCCCCCCCCCCCCCCCEECCCCCE
TVFHAGTAFNEDGQVVTAGGRVLGVMATGPTLRDALAMAYAGVDQIQFEGKTYRRDIGWR
EEEECCCEECCCCCEEEECCEEEEEEECCCHHHHHHHHHHCCCHHEEECCCHHHHCCCCC
AL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]