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The map label for this gene is metH [H]

Identifier: 51893638

GI number: 51893638

Start: 2684297

End: 2686876

Strand: Reverse

Name: metH [H]

Synonym: STH2500

Alternate gene names: 51893638

Gene position: 2686876-2684297 (Counterclockwise)

Preceding gene: 51893639

Following gene: 51893637

Centisome position: 75.34

GC content: 72.95

Gene sequence:

>2580_bases
ATGAAGCTGGAGGACATGCTGGGACGCGAGGTCCTGGTCTTCGACGGCGCCATGGGCACCATGCTCCAGGCCCAGGGGCT
GGCGCCGGGGGCGTGCCCGGACGTGTGGAACCTGGAGCGGCCGGAGGACGTGATCGCCGTCCACCGGGCGTACGTGGAGG
CCGGGGCGCAGATCCTGGAGACCAACACGTTCGGCTCCACCCCGATCCGGCTCGGCCACTACGGCCTGCAGGACCGGTGC
CGCGACATCGTCGTCGCCGGGGTGCGCTGCGCCCGGGAGGCCGCCGCGGGCCGGGCCTGGGTCGCGGGCTCGATGGGGCC
GCTGGGGGCGCTGGTGGAGCCTCTGGGGGAGCTCCCCTTCGATGAGGCCTACGCACAGTTCGCCGTCCAGGCCCGGGCGT
TTGCCGAGGCGCAGCCGGACTTCATCATCATCGAGACCATCGCCGACCTGAACGAGCTGCGGGCGGCGGTGCTGGCGTGC
AGGGACCACGCCCCGGGCATCCGGATCATCGCCCAGATCACCCTGGATCCCAGCGGCCGGGCGTTCACCGGCACCGACCC
CGAGACCGCGGCGCTGGTGCTGCAGTCGCTGGGCGCCGACGTGATCGGCTTCAACTGCTCGGTGGGCCCCGACCTGCTGG
TGGAGGCGGTCGCCCGCATGGCCCGGGTCGCCCGGGTGCCCATCTCGGTGCAGCCCAACGCCGGGCTGCCGCTGCTGCAG
CCCGACGGCGCGACCCGGTTCCCCATGGGACCTGAGGAGTTCGCGGCCTACGGGCCGAAGCTGGTGGAGGCCGGGGCGGC
GCTGGTGGGGGGCTGCTGCGGCACGACGCCGGAGCATATCCGGCGGCTGCGGGCGGCGGTGGAGGGGCTGAAGCCCCCGG
CCCGGCCCGGCCCCCTGGCCCACACGCTGGGCCTCGCCTCCCGCACCCGTTCGCTGTTCTTCGTGGAGCAGAACCTGCCG
GTGGTGATCGGCGAGCGCATCAACCCCACGGGCCGGAAGCTGCTGACCAGGGACATCCGCGAAGGCGCCTTCCAGCGGGT
ACGGGCCGAGGCGAAGCAGCAGGTGGAGGCCGGGGCCGCGGTGCTGGACGTGAACGTGGGCGTGCCCCTCATCGACGAGC
CTGCGGCCATGGCCCGGGCCGTGCGGGTGATCCAGGACGCGGTGGACGTGCCCCTCTGCCTGGACTCCCCGGACCCGGCG
GCCCTGGAGGCGGGGCTGAAGGCCTGCGTGGGCAAGCCGCTGCTCAACTCCTTCAGCCTGGAGGAAGGGCGGGCCGAGGC
GGTGCTGCCGCTGGCCAGGCGCTACGGCGCGGCGGTGCTGGGGCTCACCATCGACGAGAAGGGGATCCCCGCCTCCGCCG
AGCAGCGGCTGGCGATCGCCCGGCGGCTGGTGGCCGCGGCGGAGGCGCACGGCATCCCCCGGCACGACGTGGTCATCGAC
CCGCTGGCCCTCACCGCCGGCGCCCAGCAGGCGGAGGCGAAGGAGACGCTGAAGGCCATCCGCTGGATCACCGACGAGCT
GGGGGTCCTGGCCTCCCTGGGCGTCTCCAACATCTCCTTCGGACTGCCCAACCGGCACTTCCTCAACGCCGTGTACCTGA
GCATGGCGGTGACGGAGGGGCTCGCGATGGCGATCATGAACCCCCTGGACGAGCGGATGATGGACACCGTCCGGGCCCTG
CGGCTCTTCCTCAACCGGGACCGCAACGCCGCCGTCTACATGCAGGCGGTGGGGCCGAAGCGGCTGGTGCACACGGTGGA
GGAGGCGCTGAAGGCGGCCGCGAAGGCGGGGGAGATCGCCCCCGCTGCCGTAACGCCCTCGTCCGGCGCCGCGTCGGGCC
CTGCCCCCGCCGGGGGCCGGGACGGGGCGGCGACCCCGGCCGAGCCTGCGACACCTGCGCCGGCGGACCTCGCCGACGAG
CTCGGCCGGCGGCTCCACGACGCCATCCTCGAGGGCGACCGGGAGGCCATCGTGCCCCTGGTGGAGCAGGGGCTGGCCGA
GGGCCGGGATCCGATGGACATGCTCAACCGACATCTGATCCCCGCCATCGAGGAGGTGGGGCGCCTCTTCGGCGAGGGCA
TCTACTTCCTGCCCCAGCTCATGCTCTCCGCCCAGGCCATGAAGAAGGCGTTTGCCCGGCTGAAGCCGGAGATCCAGAAG
GCCAAGGTGGGCCAGACCGAGATCGGGACGGTGGTCCTGGCCACGGTGCAGGGCGACATCCACGACATCGGGAAGAACAT
CGTCGCGGTCCTGCTGGAGAACTACGGGTTCCGGGTGGTGGACCTGGGGCGGGACGTGAAGAACGAGGTGGTCCTGGAGG
AGGCCCGGAAGGTGGGGGCCGACATGGTGGGCCTCTCCGCGCTGATGACCACCACCATGCCCCAGATGAAGCGGGTGATC
GAGCTGTTCGCCCGGGAGGGCTTCGACTGCCCGGTCATCGTGGGTGGGGCGGCCACGACGAAGGCGTTCGCCGAGCAGAT
CGGCGCCCGGGGACACGGGCGGGACGCGCAGGAGGCGGTGGCCCTGGCGCTGGAGGTGCTGAAGGAGCGCCGGGCGGGGC
GGAAGGGGGACGCAGGGTGA

Upstream 100 bases:

>100_bases
CTACCGGGTGCGGACCAGCCGGCGGTGAGCGGCCGCTGTGTGAGGTTCAGGCGGTGGGGCCGCCGCCGGAGGCGGGAACG
GCCGGAAGGAGGAGCAGACG

Downstream 100 bases:

>100_bases
ACGTCCAGGTGAAGGCCGCGGGCCGGACGTTTTCGGTCCCCTCGGGGATGCCGCTCGCGCGGGCGCTGAACCAGCACGAG
CCCTTCGTGGAGACCCCCTG

Product: 5-methyltetrahydrofolate S-homocysteine methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 859; Mature: 859

Protein sequence:

>859_residues
MKLEDMLGREVLVFDGAMGTMLQAQGLAPGACPDVWNLERPEDVIAVHRAYVEAGAQILETNTFGSTPIRLGHYGLQDRC
RDIVVAGVRCAREAAAGRAWVAGSMGPLGALVEPLGELPFDEAYAQFAVQARAFAEAQPDFIIIETIADLNELRAAVLAC
RDHAPGIRIIAQITLDPSGRAFTGTDPETAALVLQSLGADVIGFNCSVGPDLLVEAVARMARVARVPISVQPNAGLPLLQ
PDGATRFPMGPEEFAAYGPKLVEAGAALVGGCCGTTPEHIRRLRAAVEGLKPPARPGPLAHTLGLASRTRSLFFVEQNLP
VVIGERINPTGRKLLTRDIREGAFQRVRAEAKQQVEAGAAVLDVNVGVPLIDEPAAMARAVRVIQDAVDVPLCLDSPDPA
ALEAGLKACVGKPLLNSFSLEEGRAEAVLPLARRYGAAVLGLTIDEKGIPASAEQRLAIARRLVAAAEAHGIPRHDVVID
PLALTAGAQQAEAKETLKAIRWITDELGVLASLGVSNISFGLPNRHFLNAVYLSMAVTEGLAMAIMNPLDERMMDTVRAL
RLFLNRDRNAAVYMQAVGPKRLVHTVEEALKAAAKAGEIAPAAVTPSSGAASGPAPAGGRDGAATPAEPATPAPADLADE
LGRRLHDAILEGDREAIVPLVEQGLAEGRDPMDMLNRHLIPAIEEVGRLFGEGIYFLPQLMLSAQAMKKAFARLKPEIQK
AKVGQTEIGTVVLATVQGDIHDIGKNIVAVLLENYGFRVVDLGRDVKNEVVLEEARKVGADMVGLSALMTTTMPQMKRVI
ELFAREGFDCPVIVGGAATTKAFAEQIGARGHGRDAQEAVALALEVLKERRAGRKGDAG

Sequences:

>Translated_859_residues
MKLEDMLGREVLVFDGAMGTMLQAQGLAPGACPDVWNLERPEDVIAVHRAYVEAGAQILETNTFGSTPIRLGHYGLQDRC
RDIVVAGVRCAREAAAGRAWVAGSMGPLGALVEPLGELPFDEAYAQFAVQARAFAEAQPDFIIIETIADLNELRAAVLAC
RDHAPGIRIIAQITLDPSGRAFTGTDPETAALVLQSLGADVIGFNCSVGPDLLVEAVARMARVARVPISVQPNAGLPLLQ
PDGATRFPMGPEEFAAYGPKLVEAGAALVGGCCGTTPEHIRRLRAAVEGLKPPARPGPLAHTLGLASRTRSLFFVEQNLP
VVIGERINPTGRKLLTRDIREGAFQRVRAEAKQQVEAGAAVLDVNVGVPLIDEPAAMARAVRVIQDAVDVPLCLDSPDPA
ALEAGLKACVGKPLLNSFSLEEGRAEAVLPLARRYGAAVLGLTIDEKGIPASAEQRLAIARRLVAAAEAHGIPRHDVVID
PLALTAGAQQAEAKETLKAIRWITDELGVLASLGVSNISFGLPNRHFLNAVYLSMAVTEGLAMAIMNPLDERMMDTVRAL
RLFLNRDRNAAVYMQAVGPKRLVHTVEEALKAAAKAGEIAPAAVTPSSGAASGPAPAGGRDGAATPAEPATPAPADLADE
LGRRLHDAILEGDREAIVPLVEQGLAEGRDPMDMLNRHLIPAIEEVGRLFGEGIYFLPQLMLSAQAMKKAFARLKPEIQK
AKVGQTEIGTVVLATVQGDIHDIGKNIVAVLLENYGFRVVDLGRDVKNEVVLEEARKVGADMVGLSALMTTTMPQMKRVI
ELFAREGFDCPVIVGGAATTKAFAEQIGARGHGRDAQEAVALALEVLKERRAGRKGDAG
>Mature_859_residues
MKLEDMLGREVLVFDGAMGTMLQAQGLAPGACPDVWNLERPEDVIAVHRAYVEAGAQILETNTFGSTPIRLGHYGLQDRC
RDIVVAGVRCAREAAAGRAWVAGSMGPLGALVEPLGELPFDEAYAQFAVQARAFAEAQPDFIIIETIADLNELRAAVLAC
RDHAPGIRIIAQITLDPSGRAFTGTDPETAALVLQSLGADVIGFNCSVGPDLLVEAVARMARVARVPISVQPNAGLPLLQ
PDGATRFPMGPEEFAAYGPKLVEAGAALVGGCCGTTPEHIRRLRAAVEGLKPPARPGPLAHTLGLASRTRSLFFVEQNLP
VVIGERINPTGRKLLTRDIREGAFQRVRAEAKQQVEAGAAVLDVNVGVPLIDEPAAMARAVRVIQDAVDVPLCLDSPDPA
ALEAGLKACVGKPLLNSFSLEEGRAEAVLPLARRYGAAVLGLTIDEKGIPASAEQRLAIARRLVAAAEAHGIPRHDVVID
PLALTAGAQQAEAKETLKAIRWITDELGVLASLGVSNISFGLPNRHFLNAVYLSMAVTEGLAMAIMNPLDERMMDTVRAL
RLFLNRDRNAAVYMQAVGPKRLVHTVEEALKAAAKAGEIAPAAVTPSSGAASGPAPAGGRDGAATPAEPATPAPADLADE
LGRRLHDAILEGDREAIVPLVEQGLAEGRDPMDMLNRHLIPAIEEVGRLFGEGIYFLPQLMLSAQAMKKAFARLKPEIQK
AKVGQTEIGTVVLATVQGDIHDIGKNIVAVLLENYGFRVVDLGRDVKNEVVLEEARKVGADMVGLSALMTTTMPQMKRVI
ELFAREGFDCPVIVGGAATTKAFAEQIGARGHGRDAQEAVALALEVLKERRAGRKGDAG

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=918, Percent_Identity=30.5010893246187, Blast_Score=333, Evalue=4e-91,
Organism=Homo sapiens, GI157266337, Length=391, Percent_Identity=26.8542199488491, Blast_Score=84, Evalue=8e-16,
Organism=Homo sapiens, GI13162290, Length=337, Percent_Identity=25.8160237388724, Blast_Score=78, Evalue=4e-14,
Organism=Escherichia coli, GI1790450, Length=882, Percent_Identity=33.5600907029478, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI1786456, Length=300, Percent_Identity=29.3333333333333, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17535405, Length=916, Percent_Identity=30.6768558951965, Blast_Score=327, Evalue=1e-89,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 90900; Mature: 90900

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS50970 HCY ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLEDMLGREVLVFDGAMGTMLQAQGLAPGACPDVWNLERPEDVIAVHRAYVEAGAQILE
CCCHHHHCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHEEE
TNTFGSTPIRLGHYGLQDRCRDIVVAGVRCAREAAAGRAWVAGSMGPLGALVEPLGELPF
CCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCH
DEAYAQFAVQARAFAEAQPDFIIIETIADLNELRAAVLACRDHAPGIRIIAQITLDPSGR
HHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCC
AFTGTDPETAALVLQSLGADVIGFNCSVGPDLLVEAVARMARVARVPISVQPNAGLPLLQ
EECCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCC
PDGATRFPMGPEEFAAYGPKLVEAGAALVGGCCGTTPEHIRRLRAAVEGLKPPARPGPLA
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHH
HTLGLASRTRSLFFVEQNLPVVIGERINPTGRKLLTRDIREGAFQRVRAEAKQQVEAGAA
HHHHHHHHCCEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VLDVNVGVPLIDEPAAMARAVRVIQDAVDVPLCLDSPDPAALEAGLKACVGKPLLNSFSL
EEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCHHHHCCCC
EEGRAEAVLPLARRYGAAVLGLTIDEKGIPASAEQRLAIARRLVAAAEAHGIPRHDVVID
CCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEC
PLALTAGAQQAEAKETLKAIRWITDELGVLASLGVSNISFGLPNRHFLNAVYLSMAVTEG
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LAMAIMNPLDERMMDTVRALRLFLNRDRNAAVYMQAVGPKRLVHTVEEALKAAAKAGEIA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCC
PAAVTPSSGAASGPAPAGGRDGAATPAEPATPAPADLADELGRRLHDAILEGDREAIVPL
CCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
VEQGLAEGRDPMDMLNRHLIPAIEEVGRLFGEGIYFLPQLMLSAQAMKKAFARLKPEIQK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
AKVGQTEIGTVVLATVQGDIHDIGKNIVAVLLENYGFRVVDLGRDVKNEVVLEEARKVGA
HHCCCCHHCEEEEEEECCHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHCH
DMVGLSALMTTTMPQMKRVIELFAREGFDCPVIVGGAATTKAFAEQIGARGHGRDAQEAV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHCCCCCCCCHHHHH
ALALEVLKERRAGRKGDAG
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKLEDMLGREVLVFDGAMGTMLQAQGLAPGACPDVWNLERPEDVIAVHRAYVEAGAQILE
CCCHHHHCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHEEE
TNTFGSTPIRLGHYGLQDRCRDIVVAGVRCAREAAAGRAWVAGSMGPLGALVEPLGELPF
CCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHCCCH
DEAYAQFAVQARAFAEAQPDFIIIETIADLNELRAAVLACRDHAPGIRIIAQITLDPSGR
HHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCC
AFTGTDPETAALVLQSLGADVIGFNCSVGPDLLVEAVARMARVARVPISVQPNAGLPLLQ
EECCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCC
PDGATRFPMGPEEFAAYGPKLVEAGAALVGGCCGTTPEHIRRLRAAVEGLKPPARPGPLA
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHH
HTLGLASRTRSLFFVEQNLPVVIGERINPTGRKLLTRDIREGAFQRVRAEAKQQVEAGAA
HHHHHHHHCCEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VLDVNVGVPLIDEPAAMARAVRVIQDAVDVPLCLDSPDPAALEAGLKACVGKPLLNSFSL
EEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCHHHHCCCC
EEGRAEAVLPLARRYGAAVLGLTIDEKGIPASAEQRLAIARRLVAAAEAHGIPRHDVVID
CCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEC
PLALTAGAQQAEAKETLKAIRWITDELGVLASLGVSNISFGLPNRHFLNAVYLSMAVTEG
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LAMAIMNPLDERMMDTVRALRLFLNRDRNAAVYMQAVGPKRLVHTVEEALKAAAKAGEIA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCC
PAAVTPSSGAASGPAPAGGRDGAATPAEPATPAPADLADELGRRLHDAILEGDREAIVPL
CCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
VEQGLAEGRDPMDMLNRHLIPAIEEVGRLFGEGIYFLPQLMLSAQAMKKAFARLKPEIQK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
AKVGQTEIGTVVLATVQGDIHDIGKNIVAVLLENYGFRVVDLGRDVKNEVVLEEARKVGA
HHCCCCHHCEEEEEEECCHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHCH
DMVGLSALMTTTMPQMKRVIELFAREGFDCPVIVGGAATTKAFAEQIGARGHGRDAQEAV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHCCCCCCCCHHHHH
ALALEVLKERRAGRKGDAG
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]