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The map label for this gene is iscS [H]

Identifier: 51893528

GI number: 51893528

Start: 2574086

End: 2575294

Strand: Reverse

Name: iscS [H]

Synonym: STH2390

Alternate gene names: 51893528

Gene position: 2575294-2574086 (Counterclockwise)

Preceding gene: 51893529

Following gene: 51893527

Centisome position: 72.22

GC content: 70.31

Gene sequence:

>1209_bases
ATGAGTGAGAAGGGGAGCAGAACTGCCATGCGGACGGTGTACTGTGACCACGCCGCGACCACCCGCTGTCATCCCGAGGT
GGCCCGGCTCGTCTACGAGTTCCTGACGGAGCATTACGGCAACCCTTCTTCCATCCACGCGGTCGGCCGCCGGGCCCGCA
AGGGGGTGGAGGAGGCACGGGCGCAGGTGGCCGCCCTGATCGGCGCCCAGCCCGGCGAGATCTTCTTCACCAGCGGCGGC
ACCGAGGCCGACAACTGGGCCCTGCGCGGCGCCATGGCTGCCAACCGGGCCAAGGGCAGGCACCTGATCATCTCGCCCTT
CGAGCATCACGCGGTGCTGGACACGGCTGAGGCCCTGGTGCGGGAAGGGTACGAGGTCACGCTGGTGGACGTGGAGCCGG
GCACCGGCATCATCCGGCCCGAGGCGCTGCTGGCGGCGATGCGCCCCGACACCGTGCTGATCTCGATCATGGCGGTGAAC
AACGAGATCGGCACCGTGCAGGACATCGCGGCCCTGTGCGCCGCGGCCAAGGCGAAGGATCCCGACGTCATCTTCCACAC
CGACGCCGTGCAGGCGGCGGGCTACGTCCCGCTGGACGTGAAGGCGCTCGGGGTGGACCTGCTCTCCATCTCCAGCCACA
AGATCTACGGCCCGAAGGGCGTCGGGGCGCTGTACGTGCGCAAGGGGTTCCGGTTCGGGCCCGGCCTCACCACCGGCGGC
GGGCAGGAGCGGAAGATGCGGGCCGGCACGGAGAACGTGCCCGGCATCGTGGGCTTCGGCCTGGCGGCGGAGATCGCGGC
CAGGGAGCTGGAGCGCCGGGCTGCTCACGCCCGGGAGATGCGGGACCGGTTCCTGCGGGGGGTCCTGGCGATCGAAGGCG
TGCGGCTGAACGGCGTCGACCCGTTCGTACACCCGGAGCTGCGCCACCCGGGCAACGCCAACGTCTCGGTGGACGGGATC
GAGGGCGAGGCGCTGCTGCTGCGCCTGGACATGGCGGGGGTCTACGCCTCGTCGGGGTCGGCCTGCACCTCCGGATCGCT
GCAGCCCAGCCACGTGTTGCTGGCGGTGGGCTGCAGCCGGGAGGAGGCCCAGTCGTCGCTGCGATTCTCCTTCGGGGAGG
AGAACACCGCGGAGGACGTGGACTACGCGGTCGAGCAGTTCGTCAAGTCGGTCGAGTTCTTGCGAAAGCTTGCGGCGCCG
GGTCGCTGA

Upstream 100 bases:

>100_bases
GCGCCGGCTGGGGTGCCGGTCCGGTCTTGCAGCCCGCCCACGCGCAGCGGCGGGGGCACGTCCCGTTGTGTGGCTTCCGA
AGCCCGTCTGCGGGGCCCAG

Downstream 100 bases:

>100_bases
CCCGGCGCACGTGTGAGCAGGGGCCGGGACGGGCGCGAGGCCGGTAAGAAGTGCCTGCCGGGCGCCGTTACGGTCGCACC
CGACGGGCTACACTATACGT

Product: cysteine desulfurase

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSEKGSRTAMRTVYCDHAATTRCHPEVARLVYEFLTEHYGNPSSIHAVGRRARKGVEEARAQVAALIGAQPGEIFFTSGG
TEADNWALRGAMAANRAKGRHLIISPFEHHAVLDTAEALVREGYEVTLVDVEPGTGIIRPEALLAAMRPDTVLISIMAVN
NEIGTVQDIAALCAAAKAKDPDVIFHTDAVQAAGYVPLDVKALGVDLLSISSHKIYGPKGVGALYVRKGFRFGPGLTTGG
GQERKMRAGTENVPGIVGFGLAAEIAARELERRAAHAREMRDRFLRGVLAIEGVRLNGVDPFVHPELRHPGNANVSVDGI
EGEALLLRLDMAGVYASSGSACTSGSLQPSHVLLAVGCSREEAQSSLRFSFGEENTAEDVDYAVEQFVKSVEFLRKLAAP
GR

Sequences:

>Translated_402_residues
MSEKGSRTAMRTVYCDHAATTRCHPEVARLVYEFLTEHYGNPSSIHAVGRRARKGVEEARAQVAALIGAQPGEIFFTSGG
TEADNWALRGAMAANRAKGRHLIISPFEHHAVLDTAEALVREGYEVTLVDVEPGTGIIRPEALLAAMRPDTVLISIMAVN
NEIGTVQDIAALCAAAKAKDPDVIFHTDAVQAAGYVPLDVKALGVDLLSISSHKIYGPKGVGALYVRKGFRFGPGLTTGG
GQERKMRAGTENVPGIVGFGLAAEIAARELERRAAHAREMRDRFLRGVLAIEGVRLNGVDPFVHPELRHPGNANVSVDGI
EGEALLLRLDMAGVYASSGSACTSGSLQPSHVLLAVGCSREEAQSSLRFSFGEENTAEDVDYAVEQFVKSVEFLRKLAAP
GR
>Mature_401_residues
SEKGSRTAMRTVYCDHAATTRCHPEVARLVYEFLTEHYGNPSSIHAVGRRARKGVEEARAQVAALIGAQPGEIFFTSGGT
EADNWALRGAMAANRAKGRHLIISPFEHHAVLDTAEALVREGYEVTLVDVEPGTGIIRPEALLAAMRPDTVLISIMAVNN
EIGTVQDIAALCAAAKAKDPDVIFHTDAVQAAGYVPLDVKALGVDLLSISSHKIYGPKGVGALYVRKGFRFGPGLTTGGG
QERKMRAGTENVPGIVGFGLAAEIAARELERRAAHAREMRDRFLRGVLAIEGVRLNGVDPFVHPELRHPGNANVSVDGIE
GEALLLRLDMAGVYASSGSACTSGSLQPSHVLLAVGCSREEAQSSLRFSFGEENTAEDVDYAVEQFVKSVEFLRKLAAPG
R

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=395, Percent_Identity=45.3164556962025, Blast_Score=316, Evalue=3e-86,
Organism=Homo sapiens, GI156713448, Length=422, Percent_Identity=36.7298578199052, Blast_Score=225, Evalue=7e-59,
Organism=Escherichia coli, GI48994898, Length=393, Percent_Identity=48.0916030534351, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI1787970, Length=305, Percent_Identity=28.5245901639344, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789175, Length=413, Percent_Identity=25.90799031477, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25143064, Length=404, Percent_Identity=40.3465346534653, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17533177, Length=321, Percent_Identity=31.4641744548287, Blast_Score=157, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6319831, Length=395, Percent_Identity=45.8227848101266, Blast_Score=320, Evalue=2e-88,
Organism=Drosophila melanogaster, GI20129463, Length=402, Percent_Identity=45.0248756218905, Blast_Score=294, Evalue=8e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42960; Mature: 42829

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKGSRTAMRTVYCDHAATTRCHPEVARLVYEFLTEHYGNPSSIHAVGRRARKGVEEAR
CCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AQVAALIGAQPGEIFFTSGGTEADNWALRGAMAANRAKGRHLIISPFEHHAVLDTAEALV
HHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHH
REGYEVTLVDVEPGTGIIRPEALLAAMRPDTVLISIMAVNNEIGTVQDIAALCAAAKAKD
HCCCEEEEEEECCCCCCCCHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCC
PDVIFHTDAVQAAGYVPLDVKALGVDLLSISSHKIYGPKGVGALYVRKGFRFGPGLTTGG
CCEEEECCHHHHCCCCCEEHHHHCEEEEEECCCEEECCCCCCEEEEECCCEECCCCCCCC
GQERKMRAGTENVPGIVGFGLAAEIAARELERRAAHAREMRDRFLRGVLAIEGVRLNGVD
CCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCC
PFVHPELRHPGNANVSVDGIEGEALLLRLDMAGVYASSGSACTSGSLQPSHVLLAVGCSR
CCCCCCCCCCCCCCEEEECCCCCEEEEEEEECEEEECCCCCCCCCCCCCCEEEEEECCCH
EEAQSSLRFSFGEENTAEDVDYAVEQFVKSVEFLRKLAAPGR
HHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SEKGSRTAMRTVYCDHAATTRCHPEVARLVYEFLTEHYGNPSSIHAVGRRARKGVEEAR
CCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AQVAALIGAQPGEIFFTSGGTEADNWALRGAMAANRAKGRHLIISPFEHHAVLDTAEALV
HHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHH
REGYEVTLVDVEPGTGIIRPEALLAAMRPDTVLISIMAVNNEIGTVQDIAALCAAAKAKD
HCCCEEEEEEECCCCCCCCHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCC
PDVIFHTDAVQAAGYVPLDVKALGVDLLSISSHKIYGPKGVGALYVRKGFRFGPGLTTGG
CCEEEECCHHHHCCCCCEEHHHHCEEEEEECCCEEECCCCCCEEEEECCCEECCCCCCCC
GQERKMRAGTENVPGIVGFGLAAEIAARELERRAAHAREMRDRFLRGVLAIEGVRLNGVD
CCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCC
PFVHPELRHPGNANVSVDGIEGEALLLRLDMAGVYASSGSACTSGSLQPSHVLLAVGCSR
CCCCCCCCCCCCCCEEEECCCCCEEEEEEEECEEEECCCCCCCCCCCCCCEEEEEECCCH
EEAQSSLRFSFGEENTAEDVDYAVEQFVKSVEFLRKLAAPGR
HHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]