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The map label for this gene is dacB [H]

Identifier: 51893513

GI number: 51893513

Start: 2562238

End: 2563512

Strand: Reverse

Name: dacB [H]

Synonym: STH2375

Alternate gene names: 51893513

Gene position: 2563512-2562238 (Counterclockwise)

Preceding gene: 51893514

Following gene: 51893512

Centisome position: 71.88

GC content: 69.65

Gene sequence:

>1275_bases
ATGCCATCACACAGGGAGGAGATTGCGCTGAAGCGGCTCAGGGCCATCATCGCCTGCGCTTTGCTGCTGACGCTTCTGCT
TCGCCTGCCGGCCTCGGCGGACGACACCTGGTACGAGACCAGCGCCGAATCCGGTATCCTGATGGACTGCGCCACGGGGC
AGGTGCTCTGGGAGAAGAACCCCGACGAACGCCTGGCGATTGCCTCCACCACGAAGATCATGACCGCCCTCCTGGCCATC
GAGTCCGGCCGCCTGGACGAGACCGTGACCATCCCGCCGGAGGCGGAGCTGATTGAGGGCTCCAAGGTCTACCTGACCCA
GGGGACCACGTACACGCTGCGGGAGCTGCTGGAGGCGCTGATCCTGGAGTCGGCCAACGACGCCGCGGTGGCGATCGCCA
TCTTCCTGGGCGGCAGCGAGGAGGCGTTCGTCCGGCAGATGAACGAAAAGGCCCGGGCGTTGGGCCTCACCGATACCCAG
TTCCGCAACCCGCACGGCCTGGATGAAGACGGGCACTACAGCAGCGCCCGCGACCTGGCGCGCCTCGGCCGGGTGGCCAT
GGCGGATCCGGTCTACCGGGAGATCGCGGGAACCGAGTGGCTCACGTTCCCGTGGCCCGAGATGGAGACCACCCGGGAGA
TTTACAACCGGAACCTGCTCATCCAACGCATGCCGGAGGCCACGGGCGTGAAGAACGGATACACCGAGGCCGCGCTGTTC
ACCCTGGTCGGTTCGGCCGCGGCGGACGGCCGCGAGGTCATCGGCGTGCTCCTGGGCAATCCGGACAAGGAGCAGATGTA
CGCGGACATGGAGCGGCTGCTGCGCCACGGTCTGTACGACTTCTCCCTCACCACTGTGCTTTCGGCCGGCGACCTGCCGG
GTGCTGGTGGCCGGCTGGAGGAGCCGCTGCGCGCCTGGCTGCGGGAGGGGGATGAGGTGGAAATCGCCTACCGCCGGCCG
CTGCCGGGTGAGGAGTGGCCTGAAGGGACCGTGGCGGTGGTCCAGGCCCTGGTCGATGGCCGGGTGATCGCCCATCAGCC
GGTGCTGGCGCCGGTGACGGCGTCTGCCCAGGCGGAGCCGCCGGCTCACAGCGATGGGGGCTCGGGAAAGCCCCCCGGCG
CCGGGGCCGCGGCATGGTACCAACGGGCCGGTGTCTGGATGGCGACGGGCGCGGCGCTGTGCGGTGCGGTCATCGCCACC
GACCGGTTGCAGGCCCGGCGGAGGCAGCGGCGGTTCCGGCGGCACTCGGCAGACCGCCTGTCCGCCAGCGACTGA

Upstream 100 bases:

>100_bases
GCCGGGGCGCGGAAGTGTAGGACCAGCGGCCCCCAGCAGACAGGCTGAGGACCAGCGCACCGTCGCGCTGGTCCTCATGG
CTCGGTGGGATGAGGCCCGG

Downstream 100 bases:

>100_bases
ACGCGGAGACCGCAGAGCGCAGAGCGGAGACCGCAAACCGAAACGGGAGGGGCGCGCCGCAGGGCGGCGCATCCTGGTGG
CACAGAGGCTGCAGGGCCTG

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MPSHREEIALKRLRAIIACALLLTLLLRLPASADDTWYETSAESGILMDCATGQVLWEKNPDERLAIASTTKIMTALLAI
ESGRLDETVTIPPEAELIEGSKVYLTQGTTYTLRELLEALILESANDAAVAIAIFLGGSEEAFVRQMNEKARALGLTDTQ
FRNPHGLDEDGHYSSARDLARLGRVAMADPVYREIAGTEWLTFPWPEMETTREIYNRNLLIQRMPEATGVKNGYTEAALF
TLVGSAAADGREVIGVLLGNPDKEQMYADMERLLRHGLYDFSLTTVLSAGDLPGAGGRLEEPLRAWLREGDEVEIAYRRP
LPGEEWPEGTVAVVQALVDGRVIAHQPVLAPVTASAQAEPPAHSDGGSGKPPGAGAAAWYQRAGVWMATGAALCGAVIAT
DRLQARRRQRRFRRHSADRLSASD

Sequences:

>Translated_424_residues
MPSHREEIALKRLRAIIACALLLTLLLRLPASADDTWYETSAESGILMDCATGQVLWEKNPDERLAIASTTKIMTALLAI
ESGRLDETVTIPPEAELIEGSKVYLTQGTTYTLRELLEALILESANDAAVAIAIFLGGSEEAFVRQMNEKARALGLTDTQ
FRNPHGLDEDGHYSSARDLARLGRVAMADPVYREIAGTEWLTFPWPEMETTREIYNRNLLIQRMPEATGVKNGYTEAALF
TLVGSAAADGREVIGVLLGNPDKEQMYADMERLLRHGLYDFSLTTVLSAGDLPGAGGRLEEPLRAWLREGDEVEIAYRRP
LPGEEWPEGTVAVVQALVDGRVIAHQPVLAPVTASAQAEPPAHSDGGSGKPPGAGAAAWYQRAGVWMATGAALCGAVIAT
DRLQARRRQRRFRRHSADRLSASD
>Mature_423_residues
PSHREEIALKRLRAIIACALLLTLLLRLPASADDTWYETSAESGILMDCATGQVLWEKNPDERLAIASTTKIMTALLAIE
SGRLDETVTIPPEAELIEGSKVYLTQGTTYTLRELLEALILESANDAAVAIAIFLGGSEEAFVRQMNEKARALGLTDTQF
RNPHGLDEDGHYSSARDLARLGRVAMADPVYREIAGTEWLTFPWPEMETTREIYNRNLLIQRMPEATGVKNGYTEAALFT
LVGSAAADGREVIGVLLGNPDKEQMYADMERLLRHGLYDFSLTTVLSAGDLPGAGGRLEEPLRAWLREGDEVEIAYRRPL
PGEEWPEGTVAVVQALVDGRVIAHQPVLAPVTASAQAEPPAHSDGGSGKPPGAGAAAWYQRAGVWMATGAALCGAVIATD
RLQARRRQRRFRRHSADRLSASD

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=264, Percent_Identity=35.6060606060606, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI87082022, Length=362, Percent_Identity=30.6629834254144, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1787062, Length=287, Percent_Identity=34.4947735191638, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87082054, Length=247, Percent_Identity=28.7449392712551, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 46211; Mature: 46080

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSHREEIALKRLRAIIACALLLTLLLRLPASADDTWYETSAESGILMDCATGQVLWEKN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCEEEEECCCCEEECCC
PDERLAIASTTKIMTALLAIESGRLDETVTIPPEAELIEGSKVYLTQGTTYTLRELLEAL
CCCEEEHHHHHHHHHHHHHHHCCCCCCEEECCCCHHEECCCEEEEECCCHHHHHHHHHHH
ILESANDAAVAIAIFLGGSEEAFVRQMNEKARALGLTDTQFRNPHGLDEDGHYSSARDLA
HHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
RLGRVAMADPVYREIAGTEWLTFPWPEMETTREIYNRNLLIQRMPEATGVKNGYTEAALF
HHHHHHHCCHHHHHHCCCCEEECCCCCHHHHHHHHCCCHHEEECCCCCCCCCCCHHHHHH
TLVGSAAADGREVIGVLLGNPDKEQMYADMERLLRHGLYDFSLTTVLSAGDLPGAGGRLE
HHHHHCCCCCHHEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHH
EPLRAWLREGDEVEIAYRRPLPGEEWPEGTVAVVQALVDGRVIAHQPVLAPVTASAQAEP
HHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCC
PAHSDGGSGKPPGAGAAAWYQRAGVWMATGAALCGAVIATDRLQARRRQRRFRRHSADRL
CCCCCCCCCCCCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SASD
CCCC
>Mature Secondary Structure 
PSHREEIALKRLRAIIACALLLTLLLRLPASADDTWYETSAESGILMDCATGQVLWEKN
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCEEEEECCCCEEECCC
PDERLAIASTTKIMTALLAIESGRLDETVTIPPEAELIEGSKVYLTQGTTYTLRELLEAL
CCCEEEHHHHHHHHHHHHHHHCCCCCCEEECCCCHHEECCCEEEEECCCHHHHHHHHHHH
ILESANDAAVAIAIFLGGSEEAFVRQMNEKARALGLTDTQFRNPHGLDEDGHYSSARDLA
HHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHH
RLGRVAMADPVYREIAGTEWLTFPWPEMETTREIYNRNLLIQRMPEATGVKNGYTEAALF
HHHHHHHCCHHHHHHCCCCEEECCCCCHHHHHHHHCCCHHEEECCCCCCCCCCCHHHHHH
TLVGSAAADGREVIGVLLGNPDKEQMYADMERLLRHGLYDFSLTTVLSAGDLPGAGGRLE
HHHHHCCCCCHHEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHH
EPLRAWLREGDEVEIAYRRPLPGEEWPEGTVAVVQALVDGRVIAHQPVLAPVTASAQAEP
HHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCC
PAHSDGGSGKPPGAGAAAWYQRAGVWMATGAALCGAVIATDRLQARRRQRRFRRHSADRL
CCCCCCCCCCCCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SASD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]