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The map label for this gene is dosC [H]

Identifier: 51893273

GI number: 51893273

Start: 2281469

End: 2282944

Strand: Reverse

Name: dosC [H]

Synonym: STH2135

Alternate gene names: 51893273

Gene position: 2282944-2281469 (Counterclockwise)

Preceding gene: 51893274

Following gene: 51893271

Centisome position: 64.02

GC content: 70.05

Gene sequence:

>1476_bases
ATGGGGCACAGACAACCGAACGACGTCGGCGCGGTCACCGGCCTGCACTGGAGCGGGCGGGTGTTTATGGCCGCGCTTTC
GGCGTTCGCTGTCGCCTCTGCCTTCGCCGGCTGGCTTCTGACCCGGCGGCCGCCCATGTGGTCCCTGGCCTGGTGGGGAC
CCGGAATCGGCGCTGCGCTGGGCTTCGTGCTCACGTTCAGCGCCTTCTGGCGCGTGTACGTGACCGTTCCCTGGCTGCCG
TGGGCTTTCCTGGCTCTGGACGGGCTGGCGGTGATCCTGTTCTGGTCCGATCCTGACTCGCCGTTCGGCCCGCTGCTGCT
CCTCCTGCTCTCGGCGGTGGTCGCAGCCCGGTTCCGCAAGTGGTTCCTGATGGCGCACGTATGGATCCTGGCGGTGGCCC
TGGCCGGCGCCTGGTTCCTGGGGGCCGATGCTTCCGCCCTGCTGTTCCTGGTCTCCGGGCTCAGCGGCGTGATCGGGGTT
ATCCGGGCCATCTACCTGCGCTTCCGCGAGCAGGCGATCCAGACCGACCACCTGCGGCGGCTGATGGAGCTGCTGCCGGT
GCTGAAGGCGCACGGGGTGCAGGACGTGGTGGAGGCGGCCGTGCGCCACATCGTGCGCGCGACGAACAGCCGGACGGGCG
TGGTGCTGCTCCTCGACGAGGAGGCCCAGGTGCTCCGCCCGCACTTCCTGTACAGTGAGCCGCCCCTGTCGCCTGAGGAG
AAGGAGGCGGTCATGAACACGACCATCGAGGTCGGCGTGGGGCTGTCCGGGTGGGTGGCCAAGCACGGCCAGTCCATCCT
CACCAACGACGCGGCCCGGGATCCCCGCGCCGCCCACGTGCCCGGCACGCCGCACGAGGATGAGTCGATCCTGGTGGTGC
CGCTGATCGCCGACGGCCGGCTCTACGGCGTGCTGCGGGTGGACCGGCCGGGCGTGGATCAGTACACCATCGGCGACCTG
CACCTCCTGGAACTCATGGCCGCCCACGTCTCCGATGCCATCTCCCGCGCACAGCTGGAGGAGCGGCTGGCCCGCCGGGA
CGCGCTGACCGGCGTCTACAACCGGCACTACCTCAACGAGTGGTCGCAGCACGCGATGCGGGAAGTGCGCGAGGTCTCGG
TGCTGCTGATCGACCTGCGCAAGTTCAAGGAGGTCAACGACCGCTGGGGGCACCTGACGGGCGACCGGGTCCTGCGGCAG
TGCGCGGAGCTGATCTGCGGCAGCGTGCGCACCGGGGACCTGGTGGTGCGGTACGGCGGTGATGAGTTCCTGGTGGTGCT
CGCCAACACCACCCGGGCCGAGGCGGAGAGGATCGCCGCCCGGATCCAGGAGCGGGTGGCGGAGTGGAACGCGCAGCAGG
GGGAGGGCGAGCCCCGCCTGATCCTGGACATCGGCGTCGACACCGCCCAGCAGTCGGAGTTCCAGCAGCTCCTGGACCGC
GCCGACGAGCGCATGTACGCCTGCAAGTACGCCTGA

Upstream 100 bases:

>100_bases
CCGGGCTTTGACCGTTCGCCCCCTGTTCGCCAGCAGGAAAAATGCGCAGGAGCGGCGAATCCCCCGCAGGAAGTTCGATC
GCGGAATCGGGGGTGCCGGC

Downstream 100 bases:

>100_bases
GCCGTGACGCATCCCTGAGGCGTCACGGCTCTTCCGCGCCCTCCGGCTGCATCCCCAGCGCGGCCTGCACGCGCGGCAGG
TGCTCCGCCGCGGGGGCGCC

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MGHRQPNDVGAVTGLHWSGRVFMAALSAFAVASAFAGWLLTRRPPMWSLAWWGPGIGAALGFVLTFSAFWRVYVTVPWLP
WAFLALDGLAVILFWSDPDSPFGPLLLLLLSAVVAARFRKWFLMAHVWILAVALAGAWFLGADASALLFLVSGLSGVIGV
IRAIYLRFREQAIQTDHLRRLMELLPVLKAHGVQDVVEAAVRHIVRATNSRTGVVLLLDEEAQVLRPHFLYSEPPLSPEE
KEAVMNTTIEVGVGLSGWVAKHGQSILTNDAARDPRAAHVPGTPHEDESILVVPLIADGRLYGVLRVDRPGVDQYTIGDL
HLLELMAAHVSDAISRAQLEERLARRDALTGVYNRHYLNEWSQHAMREVREVSVLLIDLRKFKEVNDRWGHLTGDRVLRQ
CAELICGSVRTGDLVVRYGGDEFLVVLANTTRAEAERIAARIQERVAEWNAQQGEGEPRLILDIGVDTAQQSEFQQLLDR
ADERMYACKYA

Sequences:

>Translated_491_residues
MGHRQPNDVGAVTGLHWSGRVFMAALSAFAVASAFAGWLLTRRPPMWSLAWWGPGIGAALGFVLTFSAFWRVYVTVPWLP
WAFLALDGLAVILFWSDPDSPFGPLLLLLLSAVVAARFRKWFLMAHVWILAVALAGAWFLGADASALLFLVSGLSGVIGV
IRAIYLRFREQAIQTDHLRRLMELLPVLKAHGVQDVVEAAVRHIVRATNSRTGVVLLLDEEAQVLRPHFLYSEPPLSPEE
KEAVMNTTIEVGVGLSGWVAKHGQSILTNDAARDPRAAHVPGTPHEDESILVVPLIADGRLYGVLRVDRPGVDQYTIGDL
HLLELMAAHVSDAISRAQLEERLARRDALTGVYNRHYLNEWSQHAMREVREVSVLLIDLRKFKEVNDRWGHLTGDRVLRQ
CAELICGSVRTGDLVVRYGGDEFLVVLANTTRAEAERIAARIQERVAEWNAQQGEGEPRLILDIGVDTAQQSEFQQLLDR
ADERMYACKYA
>Mature_490_residues
GHRQPNDVGAVTGLHWSGRVFMAALSAFAVASAFAGWLLTRRPPMWSLAWWGPGIGAALGFVLTFSAFWRVYVTVPWLPW
AFLALDGLAVILFWSDPDSPFGPLLLLLLSAVVAARFRKWFLMAHVWILAVALAGAWFLGADASALLFLVSGLSGVIGVI
RAIYLRFREQAIQTDHLRRLMELLPVLKAHGVQDVVEAAVRHIVRATNSRTGVVLLLDEEAQVLRPHFLYSEPPLSPEEK
EAVMNTTIEVGVGLSGWVAKHGQSILTNDAARDPRAAHVPGTPHEDESILVVPLIADGRLYGVLRVDRPGVDQYTIGDLH
LLELMAAHVSDAISRAQLEERLARRDALTGVYNRHYLNEWSQHAMREVREVSVLLIDLRKFKEVNDRWGHLTGDRVLRQC
AELICGSVRTGDLVVRYGGDEFLVVLANTTRAEAERIAARIQERVAEWNAQQGEGEPRLILDIGVDTAQQSEFQQLLDRA
DERMYACKYA

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=149, Percent_Identity=40.2684563758389, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787262, Length=180, Percent_Identity=31.1111111111111, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI87081881, Length=220, Percent_Identity=33.6363636363636, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI87082007, Length=153, Percent_Identity=35.9477124183007, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786584, Length=157, Percent_Identity=31.8471337579618, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787816, Length=149, Percent_Identity=34.2281879194631, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788085, Length=119, Percent_Identity=31.9327731092437, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787541, Length=114, Percent_Identity=39.4736842105263, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87081974, Length=147, Percent_Identity=31.9727891156463, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87081977, Length=173, Percent_Identity=30.635838150289, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787802, Length=183, Percent_Identity=28.9617486338798, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788381, Length=152, Percent_Identity=30.9210526315789, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1788956, Length=157, Percent_Identity=32.484076433121, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 54615; Mature: 54483

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHRQPNDVGAVTGLHWSGRVFMAALSAFAVASAFAGWLLTRRPPMWSLAWWGPGIGAAL
CCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHH
GFVLTFSAFWRVYVTVPWLPWAFLALDGLAVILFWSDPDSPFGPLLLLLLSAVVAARFRK
HHHHHHHHHHHHHEECCCHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
WFLMAHVWILAVALAGAWFLGADASALLFLVSGLSGVIGVIRAIYLRFREQAIQTDHLRR
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMELLPVLKAHGVQDVVEAAVRHIVRATNSRTGVVLLLDEEAQVLRPHFLYSEPPLSPEE
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHCCHHCCCCCCCCCCH
KEAVMNTTIEVGVGLSGWVAKHGQSILTNDAARDPRAAHVPGTPHEDESILVVPLIADGR
HHHHHHHHHHCCCCCHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCE
LYGVLRVDRPGVDQYTIGDLHLLELMAAHVSDAISRAQLEERLARRDALTGVYNRHYLNE
EEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSQHAMREVREVSVLLIDLRKFKEVNDRWGHLTGDRVLRQCAELICGSVRTGDLVVRYGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECC
DEFLVVLANTTRAEAERIAARIQERVAEWNAQQGEGEPRLILDIGVDTAQQSEFQQLLDR
CEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
ADERMYACKYA
HHHHHHHCCCC
>Mature Secondary Structure 
GHRQPNDVGAVTGLHWSGRVFMAALSAFAVASAFAGWLLTRRPPMWSLAWWGPGIGAAL
CCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHH
GFVLTFSAFWRVYVTVPWLPWAFLALDGLAVILFWSDPDSPFGPLLLLLLSAVVAARFRK
HHHHHHHHHHHHHEECCCHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
WFLMAHVWILAVALAGAWFLGADASALLFLVSGLSGVIGVIRAIYLRFREQAIQTDHLRR
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMELLPVLKAHGVQDVVEAAVRHIVRATNSRTGVVLLLDEEAQVLRPHFLYSEPPLSPEE
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHCCHHCCCCCCCCCCH
KEAVMNTTIEVGVGLSGWVAKHGQSILTNDAARDPRAAHVPGTPHEDESILVVPLIADGR
HHHHHHHHHHCCCCCHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCE
LYGVLRVDRPGVDQYTIGDLHLLELMAAHVSDAISRAQLEERLARRDALTGVYNRHYLNE
EEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSQHAMREVREVSVLLIDLRKFKEVNDRWGHLTGDRVLRQCAELICGSVRTGDLVVRYGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECC
DEFLVVLANTTRAEAERIAARIQERVAEWNAQQGEGEPRLILDIGVDTAQQSEFQQLLDR
CEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
ADERMYACKYA
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]