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The map label for this gene is leuC

Identifier: 51893254

GI number: 51893254

Start: 2258049

End: 2259494

Strand: Reverse

Name: leuC

Synonym: STH2116

Alternate gene names: 51893254

Gene position: 2259494-2258049 (Counterclockwise)

Preceding gene: 51893259

Following gene: 51893253

Centisome position: 63.36

GC content: 70.75

Gene sequence:

>1446_bases
ATGGGGCAAGGGGTGATCGGCATGAGCGGACGGACCTTGCTGGACAAGATCTGGGAGCGGCACGTGGTGCGCCGGGAGCC
CGGGAAGCCGGACCTGCTGTACATCGATCTGCACCTGGTGCACGAGGTGACCTCTCCCCAGGCCTTCGAGGGGCTGCGCA
TGGCGGGGCGGAGGGTCCGGCGGCCGGACCTGACCATCGCCACCATGGACCACAACGTGCCCACCACCGACCGGTCGCTT
CCCCTGACCGACGAGATCGCCGCGCGGCAGATGGCCGCCCTGGAGCGGAACTGCAGCGAGTTCGGGGTCAGGCTCTTTGA
CCTGTACAGCCCGTTTCAGGGCATCGTGCACGTCATCGGCCCGGAGCTGGGCCTCACCCAGCCGGGGCTGACCATCGTCT
GCGGCGACAGCCACACCGCCACCCACGGCGCCTTCGGCGCCCTGGCCTTCGGCATCGGCACCAGCGAGGTAGAGCACGTC
CTCGCCACGCAGTGTCTCTGGCAGCACAAGCCGAAGGTGATGGAGATCCGGGTGAACGGGAAGCTGCCGCCCGGGACGAC
GGCCAAGGACCTCATCCTGGGCATCATCGGCCAGATCGGCACCGACGGCGCCACCGGGTACGTGATCGAGTACACCGGCG
AGGCGATCCGGTCGCTGTCGATGGAGGGCCGAATGACCGTCTGCAACATGTCCATCGAAGCCGGAGCCCGGGCGGGGATG
ATCGCCCCGGACGAGACCACCTTCGCCTATCTGAAAGGCCGGCCTCACGCCCCCCAGGGGGAGCTGTGGGAGGCGGCGGT
GGCCGACTGGCGCACGCTGGCCTCAGACCCCGACGCCGTCTACGACCGGGTGGTGGAGTTCGACGCCGGACAGCTGGCGC
CGGTCGTCTCGTGGGGTACCAACCCCGGGCAGGTGGTGCCGGTCACCGGCCGGATCCCGGATCCCCGGGACTTCGCCGAC
CCCGCCCAGCGCAAGGCGGCGGAGGCGGCCCTGGCCTACATGGACCTGGAGCCCGGTACCCCGATCCAGGACATCCGCAT
CGACCGGGTCTTCATCGGCTCCTGCACCAACGGGCGCATCGAGGACCTGCGGGCCGCCGCGGCGGTGGTGAAGGGGCGCA
AGGTCGCCCCCGGGGTGCGGGCGATGGTCGTCCCCGGCTCCGGCCAGGTGAAGGCGCAGGCCGAGGCGGAGGGGCTGGAC
CAGATCTTCCGGGAGGCGGGCTTCGAGTGGCGGGAGGCCGGTTGCTCGATGTGCCTCGGGATGAATCCCGACATCCTGGC
CCCCGGGGAACGGTGCGCATCTACCTCCAACCGCAACTTCGAGGGCAGGCAGGGCAAGGGCGGCCGGACGCACCTGGTCA
GCCCGGCCATGGCGGCGGCCGCGGCCATCGCCGGCCACTTCGTCGACGTGCGTGAACTCCTGGCGGAAGGGGGTGCGGCC
CGGTGA

Upstream 100 bases:

>100_bases
AGCGCAGGTGGTGCAGTTCCACACGAGCACCCCCATAGGTTAAACCTATCATCATCATAGTTATCATATGTTGGAGTTCT
GGTGCAAGCTCCGGTTATAT

Downstream 100 bases:

>100_bases
GGCCCTTCACACGGGAGACGGGGCTGGCGGTGCCGCTGGACCGGGTGAACGTGGACACCGACCAGATCATCCCCAAGCAG
TTCCTGAAGCGCATCGAGCG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MGQGVIGMSGRTLLDKIWERHVVRREPGKPDLLYIDLHLVHEVTSPQAFEGLRMAGRRVRRPDLTIATMDHNVPTTDRSL
PLTDEIAARQMAALERNCSEFGVRLFDLYSPFQGIVHVIGPELGLTQPGLTIVCGDSHTATHGAFGALAFGIGTSEVEHV
LATQCLWQHKPKVMEIRVNGKLPPGTTAKDLILGIIGQIGTDGATGYVIEYTGEAIRSLSMEGRMTVCNMSIEAGARAGM
IAPDETTFAYLKGRPHAPQGELWEAAVADWRTLASDPDAVYDRVVEFDAGQLAPVVSWGTNPGQVVPVTGRIPDPRDFAD
PAQRKAAEAALAYMDLEPGTPIQDIRIDRVFIGSCTNGRIEDLRAAAAVVKGRKVAPGVRAMVVPGSGQVKAQAEAEGLD
QIFREAGFEWREAGCSMCLGMNPDILAPGERCASTSNRNFEGRQGKGGRTHLVSPAMAAAAAIAGHFVDVRELLAEGGAA
R

Sequences:

>Translated_481_residues
MGQGVIGMSGRTLLDKIWERHVVRREPGKPDLLYIDLHLVHEVTSPQAFEGLRMAGRRVRRPDLTIATMDHNVPTTDRSL
PLTDEIAARQMAALERNCSEFGVRLFDLYSPFQGIVHVIGPELGLTQPGLTIVCGDSHTATHGAFGALAFGIGTSEVEHV
LATQCLWQHKPKVMEIRVNGKLPPGTTAKDLILGIIGQIGTDGATGYVIEYTGEAIRSLSMEGRMTVCNMSIEAGARAGM
IAPDETTFAYLKGRPHAPQGELWEAAVADWRTLASDPDAVYDRVVEFDAGQLAPVVSWGTNPGQVVPVTGRIPDPRDFAD
PAQRKAAEAALAYMDLEPGTPIQDIRIDRVFIGSCTNGRIEDLRAAAAVVKGRKVAPGVRAMVVPGSGQVKAQAEAEGLD
QIFREAGFEWREAGCSMCLGMNPDILAPGERCASTSNRNFEGRQGKGGRTHLVSPAMAAAAAIAGHFVDVRELLAEGGAA
R
>Mature_480_residues
GQGVIGMSGRTLLDKIWERHVVRREPGKPDLLYIDLHLVHEVTSPQAFEGLRMAGRRVRRPDLTIATMDHNVPTTDRSLP
LTDEIAARQMAALERNCSEFGVRLFDLYSPFQGIVHVIGPELGLTQPGLTIVCGDSHTATHGAFGALAFGIGTSEVEHVL
ATQCLWQHKPKVMEIRVNGKLPPGTTAKDLILGIIGQIGTDGATGYVIEYTGEAIRSLSMEGRMTVCNMSIEAGARAGMI
APDETTFAYLKGRPHAPQGELWEAAVADWRTLASDPDAVYDRVVEFDAGQLAPVVSWGTNPGQVVPVTGRIPDPRDFADP
AQRKAAEAALAYMDLEPGTPIQDIRIDRVFIGSCTNGRIEDLRAAAAVVKGRKVAPGVRAMVVPGSGQVKAQAEAEGLDQ
IFREAGFEWREAGCSMCLGMNPDILAPGERCASTSNRNFEGRQGKGGRTHLVSPAMAAAAAIAGHFVDVRELLAEGGAAR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=370, Percent_Identity=31.3513513513514, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI8659555, Length=433, Percent_Identity=27.7136258660508, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI41352693, Length=367, Percent_Identity=27.5204359673025, Blast_Score=106, Evalue=5e-23,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=66.9546436285097, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=453, Percent_Identity=29.8013245033113, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87081781, Length=376, Percent_Identity=24.2021276595745, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI2367097, Length=334, Percent_Identity=25.748502994012, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=392, Percent_Identity=32.1428571428571, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI32564738, Length=392, Percent_Identity=32.1428571428571, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI25149342, Length=324, Percent_Identity=31.7901234567901, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17568399, Length=447, Percent_Identity=27.2930648769575, Blast_Score=118, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=64.4067796610169, Blast_Score=603, Evalue=1e-173,
Organism=Saccharomyces cerevisiae, GI6320440, Length=460, Percent_Identity=27.6086956521739, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6323335, Length=367, Percent_Identity=31.0626702997275, Blast_Score=153, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6322261, Length=397, Percent_Identity=30.2267002518892, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI281365315, Length=384, Percent_Identity=30.7291666666667, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI17864292, Length=384, Percent_Identity=30.7291666666667, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI161076999, Length=384, Percent_Identity=30.7291666666667, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI28571643, Length=400, Percent_Identity=28.75, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24645686, Length=455, Percent_Identity=27.6923076923077, Blast_Score=112, Evalue=8e-25,
Organism=Drosophila melanogaster, GI17137564, Length=443, Percent_Identity=27.765237020316, Blast_Score=111, Evalue=9e-25,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_SYMTH (Q67MJ2)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_075945.1
- ProteinModelPortal:   Q67MJ2
- SMR:   Q67MJ2
- GeneID:   2982127
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH2116
- NMPDR:   fig|292459.1.peg.2043
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   STHE292459:STH2116-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 51568; Mature: 51436

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQGVIGMSGRTLLDKIWERHVVRREPGKPDLLYIDLHLVHEVTSPQAFEGLRMAGRRVR
CCCCEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEHHCCCCHHHHHHHHHHHHCC
RPDLTIATMDHNVPTTDRSLPLTDEIAARQMAALERNCSEFGVRLFDLYSPFQGIVHVIG
CCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHCHHHHHHHHHHHHHHHHHC
PELGLTQPGLTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQCLWQHKPKVMEIRVNG
CCCCCCCCCCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECC
KLPPGTTAKDLILGIIGQIGTDGATGYVIEYTGEAIRSLSMEGRMTVCNMSIEAGARAGM
CCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHCCCCCEEEEEEEHHCCCCCCC
IAPDETTFAYLKGRPHAPQGELWEAAVADWRTLASDPDAVYDRVVEFDAGQLAPVVSWGT
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCC
NPGQVVPVTGRIPDPRDFADPAQRKAAEAALAYMDLEPGTPIQDIRIDRVFIGSCTNGRI
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEECCCCCCHHHEEEEEEEEECCCCCHH
EDLRAAAAVVKGRKVAPGVRAMVVPGSGQVKAQAEAEGLDQIFREAGFEWREAGCSMCLG
HHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHCCCHHHHCCCCEEEC
MNPDILAPGERCASTSNRNFEGRQGKGGRTHLVSPAMAAAAAIAGHFVDVRELLAEGGAA
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCC
R
C
>Mature Secondary Structure 
GQGVIGMSGRTLLDKIWERHVVRREPGKPDLLYIDLHLVHEVTSPQAFEGLRMAGRRVR
CCCEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEHHCCCCHHHHHHHHHHHHCC
RPDLTIATMDHNVPTTDRSLPLTDEIAARQMAALERNCSEFGVRLFDLYSPFQGIVHVIG
CCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHCHHHHHHHHHHHHHHHHHC
PELGLTQPGLTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQCLWQHKPKVMEIRVNG
CCCCCCCCCCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECC
KLPPGTTAKDLILGIIGQIGTDGATGYVIEYTGEAIRSLSMEGRMTVCNMSIEAGARAGM
CCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHCCCCCEEEEEEEHHCCCCCCC
IAPDETTFAYLKGRPHAPQGELWEAAVADWRTLASDPDAVYDRVVEFDAGQLAPVVSWGT
CCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCC
NPGQVVPVTGRIPDPRDFADPAQRKAAEAALAYMDLEPGTPIQDIRIDRVFIGSCTNGRI
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHEEEECCCCCCHHHEEEEEEEEECCCCCHH
EDLRAAAAVVKGRKVAPGVRAMVVPGSGQVKAQAEAEGLDQIFREAGFEWREAGCSMCLG
HHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHCCCHHHHCCCCEEEC
MNPDILAPGERCASTSNRNFEGRQGKGGRTHLVSPAMAAAAAIAGHFVDVRELLAEGGAA
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA