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The map label for this gene is miaB

Identifier: 51892897

GI number: 51892897

Start: 1905007

End: 1906419

Strand: Reverse

Name: miaB

Synonym: STH1759

Alternate gene names: 51892897

Gene position: 1906419-1905007 (Counterclockwise)

Preceding gene: 51892898

Following gene: 51892896

Centisome position: 53.46

GC content: 67.52

Gene sequence:

>1413_bases
GTGGCGCAGATGCAGATCGATCCGGAAGCGGGCCGGCGCATCCACGGCAAGCCCGAGCCCCGGGTGCACATCGAGACCTT
CGGGTGCCAGATGAACGAGCACGACAGCGAGATCATGTACGGCATCCTCGCCCAGATGGGCTACGTGAAGGCCCAGGGGC
CTGACGACGCCGACCTCCTGCTGTTCAACACCTGCGCCGTGCGGGAGTCGGCGGTGGAGCACGCCTTCGGCCGCATCGGC
CAGCTGAAGCCGCTGAAGTACACCAACCCCGACCTCATCATCGGGGTGTGCGGCTGCGTGCCCCAGGTGGAGGGCCAGGT
GGAGCGCATCAAGCGGATGTTCCCGTACCTGGACCTGATCTTCGGCACCCACAACATCCACCGGCTGCCCGAGCTGGTGG
AGCGGGCCAGGAGCGAGCGGGAGACCGTGGTGGACGTGTGGGAGTCGATGGGCGACGACTTCCCGGACATCCTGCCGGCG
GCCCGGGAGGGCGACCTGAAGGCCTGGGTCACGATCATGTACGGGTGCGACAAGCACTGCACCTACTGCATTGTGCCCAC
CACCCGCGGCAAGGAGCGCAGCCGTCCCTACGAGGTCATCCTCGCCGAGGTCCAGGAGCTGGCGCGCCAGGGCTTCAAGG
AGATCACCCTGCTGGGCCAGAACGTCAACGCGTACGGAAAGGACCTGTACGGCCGCCACGGCGAGGGGGCCTTCGACTTC
GGCGACCTGATCGAGCTCATCGACCGGAACAGCCCCGGCATCGAGCGCATCCGCTTCACCACCAACCACCCCAAGGACTT
TACCCGCAAGATGGTGGAGCAGATCGCCCGCGCCGAGAAGGTCTGCGAGTGGTTCCACCTGCCGGTGCAGTCGGGCTCCG
ACAGCGTGCTCCGGCGCATGAAGCGCTCGTACAACCGGAAGCAGTACCTGCGGCTGGTCGGGTGGATCCGGGAGCTGATC
CCCGACGCGGTGATCACCACCGACATCATCGTGGGCTTCCCCGGCGAGACGGAGGAGGAGTTCCAGGAGACGCTGAGCCT
CGTGGAGGAGGTCCAGTACGACGCCGCCTTCATGTTCATGTACTCCGAGCGGGCCGGCACCCCGGCCGCCCAGATGGAGG
ACCGGCTCAGCGTGCCCGAGAAGAAGGAGCGGCTGCAGCGGCTGATGGAGGTGCAGAACCGCATCGCCCGCGCCAAGAAC
GAGGCGCGGGTCGGCAAGGTGTACGACATCCTGGTGGAGGGGCTGGACAAGGGCAAGCCCGACGTGGTCTTCGGGTGGAC
CCGGGGCAACATCCTGGTCACGTTCCCCGGCGACGAGTCCCTCAGGGGCCGGATCGTGCCCGTGCGCATCACCCGCGCCG
GCACCTGGACCCTGGAGGGCGAGCTGGTGGAGAGCCCGGTGACGCTGGCCTAG

Upstream 100 bases:

>100_bases
GATGATGAAGGACGGCACCCTGGAGCTGCTGCCCATGGCGGAGCAGGGGGCGACCGAGGACCTGACGCCCGGCGGCTTCG
ACCACCTGGAGCCCGCCGGG

Downstream 100 bases:

>100_bases
TCAGCACGATGGGGGGAGATGACGTGGCGGTTGCGACCCGTCGCGATGTGTGGTTGCTGGCCCGGGAGCTGGCCGAGGCC
ATCGCGGAGACGCCGGAGGT

Product: 2-methylthioadenine synthetase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MAQMQIDPEAGRRIHGKPEPRVHIETFGCQMNEHDSEIMYGILAQMGYVKAQGPDDADLLLFNTCAVRESAVEHAFGRIG
QLKPLKYTNPDLIIGVCGCVPQVEGQVERIKRMFPYLDLIFGTHNIHRLPELVERARSERETVVDVWESMGDDFPDILPA
AREGDLKAWVTIMYGCDKHCTYCIVPTTRGKERSRPYEVILAEVQELARQGFKEITLLGQNVNAYGKDLYGRHGEGAFDF
GDLIELIDRNSPGIERIRFTTNHPKDFTRKMVEQIARAEKVCEWFHLPVQSGSDSVLRRMKRSYNRKQYLRLVGWIRELI
PDAVITTDIIVGFPGETEEEFQETLSLVEEVQYDAAFMFMYSERAGTPAAQMEDRLSVPEKKERLQRLMEVQNRIARAKN
EARVGKVYDILVEGLDKGKPDVVFGWTRGNILVTFPGDESLRGRIVPVRITRAGTWTLEGELVESPVTLA

Sequences:

>Translated_470_residues
MAQMQIDPEAGRRIHGKPEPRVHIETFGCQMNEHDSEIMYGILAQMGYVKAQGPDDADLLLFNTCAVRESAVEHAFGRIG
QLKPLKYTNPDLIIGVCGCVPQVEGQVERIKRMFPYLDLIFGTHNIHRLPELVERARSERETVVDVWESMGDDFPDILPA
AREGDLKAWVTIMYGCDKHCTYCIVPTTRGKERSRPYEVILAEVQELARQGFKEITLLGQNVNAYGKDLYGRHGEGAFDF
GDLIELIDRNSPGIERIRFTTNHPKDFTRKMVEQIARAEKVCEWFHLPVQSGSDSVLRRMKRSYNRKQYLRLVGWIRELI
PDAVITTDIIVGFPGETEEEFQETLSLVEEVQYDAAFMFMYSERAGTPAAQMEDRLSVPEKKERLQRLMEVQNRIARAKN
EARVGKVYDILVEGLDKGKPDVVFGWTRGNILVTFPGDESLRGRIVPVRITRAGTWTLEGELVESPVTLA
>Mature_469_residues
AQMQIDPEAGRRIHGKPEPRVHIETFGCQMNEHDSEIMYGILAQMGYVKAQGPDDADLLLFNTCAVRESAVEHAFGRIGQ
LKPLKYTNPDLIIGVCGCVPQVEGQVERIKRMFPYLDLIFGTHNIHRLPELVERARSERETVVDVWESMGDDFPDILPAA
REGDLKAWVTIMYGCDKHCTYCIVPTTRGKERSRPYEVILAEVQELARQGFKEITLLGQNVNAYGKDLYGRHGEGAFDFG
DLIELIDRNSPGIERIRFTTNHPKDFTRKMVEQIARAEKVCEWFHLPVQSGSDSVLRRMKRSYNRKQYLRLVGWIRELIP
DAVITTDIIVGFPGETEEEFQETLSLVEEVQYDAAFMFMYSERAGTPAAQMEDRLSVPEKKERLQRLMEVQNRIARAKNE
ARVGKVYDILVEGLDKGKPDVVFGWTRGNILVTFPGDESLRGRIVPVRITRAGTWTLEGELVESPVTLA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=38.4615384615385, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI28872782, Length=479, Percent_Identity=37.9958246346555, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI93277076, Length=452, Percent_Identity=31.6371681415929, Blast_Score=188, Evalue=9e-48,
Organism=Escherichia coli, GI1786882, Length=452, Percent_Identity=43.3628318584071, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI1787057, Length=409, Percent_Identity=29.3398533007335, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17553146, Length=470, Percent_Identity=35.531914893617, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI71996771, Length=308, Percent_Identity=28.5714285714286, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI21356207, Length=480, Percent_Identity=37.2916666666667, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI19922432, Length=471, Percent_Identity=31.8471337579618, Blast_Score=191, Evalue=8e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_SYMTH (Q67NJ9)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_075588.1
- ProteinModelPortal:   Q67NJ9
- SMR:   Q67NJ9
- GeneID:   2979304
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH1759
- NMPDR:   fig|292459.1.peg.1696
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- BioCyc:   STHE292459:STH1759-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 53518; Mature: 53387

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQMQIDPEAGRRIHGKPEPRVHIETFGCQMNEHDSEIMYGILAQMGYVKAQGPDDADLL
CCCCCCCHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEECCCCCCCCEE
LFNTCAVRESAVEHAFGRIGQLKPLKYTNPDLIIGVCGCVPQVEGQVERIKRMFPYLDLI
EEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHH
FGTHNIHRLPELVERARSERETVVDVWESMGDDFPDILPAAREGDLKAWVTIMYGCDKHC
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHEEEEEEECCCCCC
TYCIVPTTRGKERSRPYEVILAEVQELARQGFKEITLLGQNVNAYGKDLYGRHGEGAFDF
EEEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHEEEHCCCCCCHHHHHHCCCCCCCCCCH
GDLIELIDRNSPGIERIRFTTNHPKDFTRKMVEQIARAEKVCEWFHLPVQSGSDSVLRRM
HHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
KRSYNRKQYLRLVGWIRELIPDAVITTDIIVGFPGETEEEFQETLSLVEEVQYDAAFMFM
HHHHCHHHHHHHHHHHHHHCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEE
YSERAGTPAAQMEDRLSVPEKKERLQRLMEVQNRIARAKNEARVGKVYDILVEGLDKGKP
EECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DVVFGWTRGNILVTFPGDESLRGRIVPVRITRAGTWTLEGELVESPVTLA
CEEEEEECCCEEEECCCCCCCCCEEEEEEEEECCEEEECCHHHCCCCCCC
>Mature Secondary Structure 
AQMQIDPEAGRRIHGKPEPRVHIETFGCQMNEHDSEIMYGILAQMGYVKAQGPDDADLL
CCCCCCHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEECCCCCCCCEE
LFNTCAVRESAVEHAFGRIGQLKPLKYTNPDLIIGVCGCVPQVEGQVERIKRMFPYLDLI
EEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHH
FGTHNIHRLPELVERARSERETVVDVWESMGDDFPDILPAAREGDLKAWVTIMYGCDKHC
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHEEEEEEECCCCCC
TYCIVPTTRGKERSRPYEVILAEVQELARQGFKEITLLGQNVNAYGKDLYGRHGEGAFDF
EEEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHEEEHCCCCCCHHHHHHCCCCCCCCCCH
GDLIELIDRNSPGIERIRFTTNHPKDFTRKMVEQIARAEKVCEWFHLPVQSGSDSVLRRM
HHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
KRSYNRKQYLRLVGWIRELIPDAVITTDIIVGFPGETEEEFQETLSLVEEVQYDAAFMFM
HHHHCHHHHHHHHHHHHHHCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEE
YSERAGTPAAQMEDRLSVPEKKERLQRLMEVQNRIARAKNEARVGKVYDILVEGLDKGKP
EECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DVVFGWTRGNILVTFPGDESLRGRIVPVRITRAGTWTLEGELVESPVTLA
CEEEEEECCCEEEECCCCCCCCCEEEEEEEEECCEEEECCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA