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The map label for this gene is iaaH [H]

Identifier: 51892828

GI number: 51892828

Start: 1836740

End: 1838080

Strand: Reverse

Name: iaaH [H]

Synonym: STH1690

Alternate gene names: 51892828

Gene position: 1838080-1836740 (Counterclockwise)

Preceding gene: 51892829

Following gene: 51892827

Centisome position: 51.54

GC content: 72.93

Gene sequence:

>1341_bases
TTGAAGGAGGTGTTACCCGTGGACATTTCAACCCGCGTCCAGCAACTGGCTCCGGACCTCGTCCGGGTGCGCCGCGACCT
GCACGCACACGCGGAGAGCGGCTGGACGGAGTTCCGCACCGCGGCGCTGGTGGTGCAGCGGCTCAGCGCCCTGGGTTACG
AAGTCCGCTACGGCCCCGAGGTGCTGAGCCCGGACCACATGGCGGGCCTGCCGCCCCGGGAGGAGCTGGACCGGCACCTG
GAGCGCGCCGTCGCCCAGGGAGCCGACCCGGAGGTGGTCCGCCGGATGGCCGGGGGCTTCACCGCCGTCGTCGGCGTGCT
CCGCTGCGGCGAGGGTCCCACCGTCGCCCTCCGGTTCGACATGGACGCCCTGGACGTGACCGAGACGGATGACCCCGGCC
ACCGGCCGAACCGGGAGGGCTTCGCCTCCCGCAATCCGGGGGCCATGCACGCCTGTGGCCACGACGGCCACACGGCCATC
GGGCTGGGGGTGGCCGCCGTCCTGGCTGACCTGAAGGACGAGCTGCGCGGCACCGTCAAGCTGCTCTTCCAGCCCGCCGA
GGAGGGCGTCCGCGGCGCCCGGGCCATGGTGGAGGCCGGGGTCTGCGACGACGTGGACTACCTGCTGGGCGCGCACCTGG
GCTTCGCCGCGACCGAGGTCGGCACGGTGATCGCCGGCGCGGGCGGCTTCCTGGCCACTTCGAAGCTGGACGCGGTCTTC
ACGGGCGTCCCCTCCCATGCGGGAGCGGCCCCGGAGGCCGGCCGAAACGCCCTGCTGGCCGCCGCCGCGGCCGCCCTCAA
CCTGCACGCGATCGCCCGCCACTCCAAAGGCGCGTCCCGGATCAACGTGGGCGTGCTGCAGGCCGGATCCGGCCGCAACG
TCATCCCCGCCCGCGCGGTCATGAAGCTGGAGACGCGCGGCGAGACGACGGCGATCGACGCCTACATGCGGGAGGAGGCG
GAGCGCATTATCCGGGCGGCCGCCGCAATGCACGGCGTGCAGGTGGAGATCCTGCCGATAGGCAGTGCCGCAGGCGGCGC
CAGCGACCCTGACCTGGCCGAGGTCGTCGCCCGGGCCGCCGCGGAGACGCCTTCGGTGACCAGGATCCTGCCTGCCGGCG
ACCTGGGAGGCAGCGAGGACTTCGCCTGGATGATGGACCGCGTGCAGAGGCGGGGCGGCAAGGCGGTCTACATGATGATC
GGCACGCAGATCGCGGCCCCTCATCACGATTCCCGGTTCGACTTTGACGAGCGGGCCCTGCCGATCGGCGTGGAGGTGAT
GGCCCGGGCCGTGCTGCGGCTGGCCGGGGGCGGCATCCCGCAAAACTCCCGGAAGAGGTGA

Upstream 100 bases:

>100_bases
GTTGGGCCCTCCGGGACGCAGCGGCCCGGCGCCTCTCGGTCCACCGTCGCACACCTTCCCCCGGGCTTTCGCAAACGCCA
GCGGTGACGTATAATCCTGG

Downstream 100 bases:

>100_bases
CCTGTGATGACCGAGCGCACCCGTCTGCTGGACACGTTTCTGACTCTGGTGACCACGGACACTCAGACGAAGCGAGAGGG
CCGGATGGCCGACCTGTTGG

Product: putative amino acid amidohydrolase

Products: NA

Alternate protein names: IAA-Asp hydrolase [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKEVLPVDISTRVQQLAPDLVRVRRDLHAHAESGWTEFRTAALVVQRLSALGYEVRYGPEVLSPDHMAGLPPREELDRHL
ERAVAQGADPEVVRRMAGGFTAVVGVLRCGEGPTVALRFDMDALDVTETDDPGHRPNREGFASRNPGAMHACGHDGHTAI
GLGVAAVLADLKDELRGTVKLLFQPAEEGVRGARAMVEAGVCDDVDYLLGAHLGFAATEVGTVIAGAGGFLATSKLDAVF
TGVPSHAGAAPEAGRNALLAAAAAALNLHAIARHSKGASRINVGVLQAGSGRNVIPARAVMKLETRGETTAIDAYMREEA
ERIIRAAAAMHGVQVEILPIGSAAGGASDPDLAEVVARAAAETPSVTRILPAGDLGGSEDFAWMMDRVQRRGGKAVYMMI
GTQIAAPHHDSRFDFDERALPIGVEVMARAVLRLAGGGIPQNSRKR

Sequences:

>Translated_446_residues
MKEVLPVDISTRVQQLAPDLVRVRRDLHAHAESGWTEFRTAALVVQRLSALGYEVRYGPEVLSPDHMAGLPPREELDRHL
ERAVAQGADPEVVRRMAGGFTAVVGVLRCGEGPTVALRFDMDALDVTETDDPGHRPNREGFASRNPGAMHACGHDGHTAI
GLGVAAVLADLKDELRGTVKLLFQPAEEGVRGARAMVEAGVCDDVDYLLGAHLGFAATEVGTVIAGAGGFLATSKLDAVF
TGVPSHAGAAPEAGRNALLAAAAAALNLHAIARHSKGASRINVGVLQAGSGRNVIPARAVMKLETRGETTAIDAYMREEA
ERIIRAAAAMHGVQVEILPIGSAAGGASDPDLAEVVARAAAETPSVTRILPAGDLGGSEDFAWMMDRVQRRGGKAVYMMI
GTQIAAPHHDSRFDFDERALPIGVEVMARAVLRLAGGGIPQNSRKR
>Mature_446_residues
MKEVLPVDISTRVQQLAPDLVRVRRDLHAHAESGWTEFRTAALVVQRLSALGYEVRYGPEVLSPDHMAGLPPREELDRHL
ERAVAQGADPEVVRRMAGGFTAVVGVLRCGEGPTVALRFDMDALDVTETDDPGHRPNREGFASRNPGAMHACGHDGHTAI
GLGVAAVLADLKDELRGTVKLLFQPAEEGVRGARAMVEAGVCDDVDYLLGAHLGFAATEVGTVIAGAGGFLATSKLDAVF
TGVPSHAGAAPEAGRNALLAAAAAALNLHAIARHSKGASRINVGVLQAGSGRNVIPARAVMKLETRGETTAIDAYMREEA
ERIIRAAAAMHGVQVEILPIGSAAGGASDPDLAEVVARAAAETPSVTRILPAGDLGGSEDFAWMMDRVQRRGGKAVYMMI
GTQIAAPHHDSRFDFDERALPIGVEVMARAVLRLAGGGIPQNSRKR

Specific function: Hydrolyzes indole-3-acetyl-N-aspartic acid to indole-3- acetic acid [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=429, Percent_Identity=51.5151515151515, Blast_Score=382, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: NA

Molecular weight: Translated: 46943; Mature: 46943

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEVLPVDISTRVQQLAPDLVRVRRDLHAHAESGWTEFRTAALVVQRLSALGYEVRYGPE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCC
VLSPDHMAGLPPREELDRHLERAVAQGADPEVVRRMAGGFTAVVGVLRCGEGPTVALRFD
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEC
MDALDVTETDDPGHRPNREGFASRNPGAMHACGHDGHTAIGLGVAAVLADLKDELRGTVK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLFQPAEEGVRGARAMVEAGVCDDVDYLLGAHLGFAATEVGTVIAGAGGFLATSKLDAVF
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
TGVPSHAGAAPEAGRNALLAAAAAALNLHAIARHSKGASRINVGVLQAGSGRNVIPARAV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHH
MKLETRGETTAIDAYMREEAERIIRAAAAMHGVQVEILPIGSAAGGASDPDLAEVVARAA
HEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHH
AETPSVTRILPAGDLGGSEDFAWMMDRVQRRGGKAVYMMIGTQIAAPHHDSRFDFDERAL
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCEEECCCCCCCCCCCCCCC
PIGVEVMARAVLRLAGGGIPQNSRKR
CCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKEVLPVDISTRVQQLAPDLVRVRRDLHAHAESGWTEFRTAALVVQRLSALGYEVRYGPE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCC
VLSPDHMAGLPPREELDRHLERAVAQGADPEVVRRMAGGFTAVVGVLRCGEGPTVALRFD
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHCCCCCEEEEEEC
MDALDVTETDDPGHRPNREGFASRNPGAMHACGHDGHTAIGLGVAAVLADLKDELRGTVK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLFQPAEEGVRGARAMVEAGVCDDVDYLLGAHLGFAATEVGTVIAGAGGFLATSKLDAVF
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
TGVPSHAGAAPEAGRNALLAAAAAALNLHAIARHSKGASRINVGVLQAGSGRNVIPARAV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHH
MKLETRGETTAIDAYMREEAERIIRAAAAMHGVQVEILPIGSAAGGASDPDLAEVVARAA
HEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHH
AETPSVTRILPAGDLGGSEDFAWMMDRVQRRGGKAVYMMIGTQIAAPHHDSRFDFDERAL
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCEEECCCCCCCCCCCCCCC
PIGVEVMARAVLRLAGGGIPQNSRKR
CCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9747708 [H]