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The map label for this gene is glpK

Identifier: 51892333

GI number: 51892333

Start: 1325225

End: 1326721

Strand: Reverse

Name: glpK

Synonym: STH1195

Alternate gene names: 51892333

Gene position: 1326721-1325225 (Counterclockwise)

Preceding gene: 51892334

Following gene: 51892332

Centisome position: 37.2

GC content: 70.61

Gene sequence:

>1497_bases
ATGAACAAGGGTTACGTCCTCGCCCTGGACCAGGGAACCACCTCTTCCCGGGCCATCCTCTTCAACCGGGACGGCCGGCT
CGTGGGGCAGGTGAACCATGAGTTCCGGCAGATCTACCCGCAGCCCGGCTGGGTCGAGCACGACCCCCGGGAGATCTGGT
CCACCCAGCTGCGCGCCGTCCAGGACGTGCTGGCCCGCACCGGCGTCCGGCTGAGCGAGGTGGCCGCGATCGGCATCACC
AACCAGCGGGAGACGACGGTGGTGTGGGACGCCGAAACCGGCGAGCCCGTGTACAACGCGATCGTCTGGCAGTGCCGCCG
CACCGCCCCCATCTGCGCCGAGCTGGCGGCGCGGGGCTGGGCGCCGCGCATCCGGGAGAAGACCGGCCTGGTCATCGACG
CCTACTTCTCGGGAACCAAAGTGAAGTGGATTCTGGACAACGTCCCCGGCGTGCGGGAGAAGGCGGAGCAGGGGCGGCTG
CGCTTCGGCACCATCGACGCCTGGCTCATCTGGAACCTGACCGGCGGGCGGGTGCACGCCACCGACTACTCCAACGCCTC
CCGCACCATGCTGTTCAATATCCACCAGCTCGCGTGGGACGAGGAGATCCTGCACGAACTCGGCATCCCGGCGTCGCTGC
TACCCCGGGCCCTCCCCTCCAGCCACGTCTACGGGGAGACCGATCCCGCGGTGCTCGGCGCGGCCATCCCCATCGCCGGC
GTGGCGGGGGACCAGCAGGCGGCGCTCTTCGGGCAGGCCTGCTTCCAGCCGGGCGATGCCAAGAACACCTACGGGACCGG
CTGCTTCATGCTGATGAACACCGGCGACCGGGCAGTGCCGTCGGAGAGCGGTCTGCTCACCACCATCGCCTGGGGCATCG
GCGACCGGGTGGAGTATGCGCTGGAGGGCTCGATCTTCATCGGCGGCGCGGCGGTGCAGTGGCTGCGGGACGAGCTCCGC
TTCTTCGACCGCGCGGCCGATTCCGAGGCCCTGGCCCTCTCCGTCCCCGACTCGGGCGGGGTGTACGTGGTGCCGGCCTT
CGTGGGCCTCGGAGCTCCGTACTGGGACATGTACGCCCGCGGCATCGTCGTGGGCCTCACCCGGGGCACGGGCCGGGCCC
ACATCACCCGGGCCACGCTGGAGTCCATTGCGTACCAGACCCGCGACGTGCTGGGGGCGATGGAGAAGGACTCGGGCATC
CGGCTGAACCGGCTGAAGGTGGACGGCGGTGCCGTCGCCAACAACTTCCTCATGCAGTTCCAGTCCGACATCCTCGGCGT
ACCGGTCGAGCGGCCGAAGATCGCGGAAACCACGGCCCTGGGTGCGGCCTACCTGGCCGGCCTGGCGACCGGCTTCTGGC
GCAGCCAGGCGGAACTGGCCGACAAGTGGGCCCTGGACCGGGGCTTCACCCCGGCCCTGCCGGCCGAGGAGAGAGAACGG
CTCTACGCCGGCTGGCGACGGGCGGTGGAGCGCGCCCGGGGCTGGGCCGCCGAGTAG

Upstream 100 bases:

>100_bases
GCAGGGCATGACGCGCCAGGCCGGAACCCCCGACGGTTCCGGCCTGGCGCCTATAAGGCCGGGGCGGCGCAAGTCCCCGG
CCGGATGGGAGGCTGCTTGC

Downstream 100 bases:

>100_bases
ACACAGGCGCACGGAGAAGGCCGGGGGCCGAAGCCCCCGGCCTCAGGTTGAAGACAAAATCCGGGTAACCCCTCGCGGGT
GCCCGGATTTTGTTGTACAA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MNKGYVLALDQGTTSSRAILFNRDGRLVGQVNHEFRQIYPQPGWVEHDPREIWSTQLRAVQDVLARTGVRLSEVAAIGIT
NQRETTVVWDAETGEPVYNAIVWQCRRTAPICAELAARGWAPRIREKTGLVIDAYFSGTKVKWILDNVPGVREKAEQGRL
RFGTIDAWLIWNLTGGRVHATDYSNASRTMLFNIHQLAWDEEILHELGIPASLLPRALPSSHVYGETDPAVLGAAIPIAG
VAGDQQAALFGQACFQPGDAKNTYGTGCFMLMNTGDRAVPSESGLLTTIAWGIGDRVEYALEGSIFIGGAAVQWLRDELR
FFDRAADSEALALSVPDSGGVYVVPAFVGLGAPYWDMYARGIVVGLTRGTGRAHITRATLESIAYQTRDVLGAMEKDSGI
RLNRLKVDGGAVANNFLMQFQSDILGVPVERPKIAETTALGAAYLAGLATGFWRSQAELADKWALDRGFTPALPAEERER
LYAGWRRAVERARGWAAE

Sequences:

>Translated_498_residues
MNKGYVLALDQGTTSSRAILFNRDGRLVGQVNHEFRQIYPQPGWVEHDPREIWSTQLRAVQDVLARTGVRLSEVAAIGIT
NQRETTVVWDAETGEPVYNAIVWQCRRTAPICAELAARGWAPRIREKTGLVIDAYFSGTKVKWILDNVPGVREKAEQGRL
RFGTIDAWLIWNLTGGRVHATDYSNASRTMLFNIHQLAWDEEILHELGIPASLLPRALPSSHVYGETDPAVLGAAIPIAG
VAGDQQAALFGQACFQPGDAKNTYGTGCFMLMNTGDRAVPSESGLLTTIAWGIGDRVEYALEGSIFIGGAAVQWLRDELR
FFDRAADSEALALSVPDSGGVYVVPAFVGLGAPYWDMYARGIVVGLTRGTGRAHITRATLESIAYQTRDVLGAMEKDSGI
RLNRLKVDGGAVANNFLMQFQSDILGVPVERPKIAETTALGAAYLAGLATGFWRSQAELADKWALDRGFTPALPAEERER
LYAGWRRAVERARGWAAE
>Mature_498_residues
MNKGYVLALDQGTTSSRAILFNRDGRLVGQVNHEFRQIYPQPGWVEHDPREIWSTQLRAVQDVLARTGVRLSEVAAIGIT
NQRETTVVWDAETGEPVYNAIVWQCRRTAPICAELAARGWAPRIREKTGLVIDAYFSGTKVKWILDNVPGVREKAEQGRL
RFGTIDAWLIWNLTGGRVHATDYSNASRTMLFNIHQLAWDEEILHELGIPASLLPRALPSSHVYGETDPAVLGAAIPIAG
VAGDQQAALFGQACFQPGDAKNTYGTGCFMLMNTGDRAVPSESGLLTTIAWGIGDRVEYALEGSIFIGGAAVQWLRDELR
FFDRAADSEALALSVPDSGGVYVVPAFVGLGAPYWDMYARGIVVGLTRGTGRAHITRATLESIAYQTRDVLGAMEKDSGI
RLNRLKVDGGAVANNFLMQFQSDILGVPVERPKIAETTALGAAYLAGLATGFWRSQAELADKWALDRGFTPALPAEERER
LYAGWRRAVERARGWAAE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=47.4103585657371, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI4504007, Length=501, Percent_Identity=47.5049900199601, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI42794763, Length=506, Percent_Identity=47.2332015810277, Blast_Score=453, Evalue=1e-127,
Organism=Homo sapiens, GI41393575, Length=506, Percent_Identity=47.2332015810277, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI88196792, Length=508, Percent_Identity=35.0393700787402, Blast_Score=342, Evalue=4e-94,
Organism=Escherichia coli, GI1790361, Length=494, Percent_Identity=63.5627530364373, Blast_Score=656, Evalue=0.0,
Organism=Escherichia coli, GI1789168, Length=484, Percent_Identity=25.4132231404959, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1789987, Length=491, Percent_Identity=24.0325865580448, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1790005, Length=478, Percent_Identity=24.6861924686192, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1787789, Length=508, Percent_Identity=24.8031496062992, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1789138, Length=508, Percent_Identity=21.259842519685, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535599, Length=500, Percent_Identity=43, Blast_Score=396, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71986140, Length=523, Percent_Identity=25.4302103250478, Blast_Score=186, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI71986147, Length=273, Percent_Identity=32.6007326007326, Blast_Score=143, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6321755, Length=548, Percent_Identity=36.4963503649635, Blast_Score=312, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24655555, Length=505, Percent_Identity=41.980198019802, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24655560, Length=505, Percent_Identity=41.980198019802, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24655570, Length=505, Percent_Identity=41.980198019802, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24655574, Length=505, Percent_Identity=41.980198019802, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24655565, Length=505, Percent_Identity=41.980198019802, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI17864214, Length=508, Percent_Identity=45.2755905511811, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24654573, Length=444, Percent_Identity=46.8468468468468, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI19922058, Length=526, Percent_Identity=32.6996197718631, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24652871, Length=492, Percent_Identity=33.3333333333333, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI21357867, Length=529, Percent_Identity=28.9224952741021, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24656367, Length=515, Percent_Identity=29.9029126213592, Blast_Score=235, Evalue=5e-62,
Organism=Drosophila melanogaster, GI24656372, Length=369, Percent_Identity=32.520325203252, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI45552909, Length=308, Percent_Identity=31.1688311688312, Blast_Score=159, Evalue=4e-39,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_SYMTH (Q67Q63)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_075024.1
- HSSP:   P0A6F3
- ProteinModelPortal:   Q67Q63
- SMR:   Q67Q63
- GeneID:   2982322
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH1195
- NMPDR:   fig|292459.1.peg.1148
- HOGENOM:   HBG511469
- OMA:   KPSSEVY
- BioCyc:   STHE292459:STH1195-MONOMER
- BRENDA:   2.7.1.30
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 54500; Mature: 54500

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 13-13 BINDING 17-17 BINDING 83-83 BINDING 135-135 BINDING 244-244 BINDING 266-266 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKGYVLALDQGTTSSRAILFNRDGRLVGQVNHEFRQIYPQPGWVEHDPREIWSTQLRAV
CCCCEEEEEECCCCCCEEEEEECCCCEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHH
QDVLARTGVRLSEVAAIGITNQRETTVVWDAETGEPVYNAIVWQCRRTAPICAELAARGW
HHHHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHCCC
APRIREKTGLVIDAYFSGTKVKWILDNVPGVREKAEQGRLRFGTIDAWLIWNLTGGRVHA
CCCHHCCCCEEEEEEECCCEEEEEECCCCCHHHHHHCCCEEEEEEEEEEEEECCCCEEEE
TDYSNASRTMLFNIHQLAWDEEILHELGIPASLLPRALPSSHVYGETDPAVLGAAIPIAG
CCCCCCCCEEEEEHHHHHCCHHHHHHCCCCHHHHHHCCCCCCCCCCCCHHHHHHHCCEEC
VAGDQQAALFGQACFQPGDAKNTYGTGCFMLMNTGDRAVPSESGLLTTIAWGIGDRVEYA
CCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCEEEE
LEGSIFIGGAAVQWLRDELRFFDRAADSEALALSVPDSGGVYVVPAFVGLGAPYWDMYAR
ECCCEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEHHHHCCCCHHHHHHC
GIVVGLTRGTGRAHITRATLESIAYQTRDVLGAMEKDSGIRLNRLKVDGGAVANNFLMQF
EEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHH
QSDILGVPVERPKIAETTALGAAYLAGLATGFWRSQAELADKWALDRGFTPALPAEERER
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LYAGWRRAVERARGWAAE
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNKGYVLALDQGTTSSRAILFNRDGRLVGQVNHEFRQIYPQPGWVEHDPREIWSTQLRAV
CCCCEEEEEECCCCCCEEEEEECCCCEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHH
QDVLARTGVRLSEVAAIGITNQRETTVVWDAETGEPVYNAIVWQCRRTAPICAELAARGW
HHHHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHCCC
APRIREKTGLVIDAYFSGTKVKWILDNVPGVREKAEQGRLRFGTIDAWLIWNLTGGRVHA
CCCHHCCCCEEEEEEECCCEEEEEECCCCCHHHHHHCCCEEEEEEEEEEEEECCCCEEEE
TDYSNASRTMLFNIHQLAWDEEILHELGIPASLLPRALPSSHVYGETDPAVLGAAIPIAG
CCCCCCCCEEEEEHHHHHCCHHHHHHCCCCHHHHHHCCCCCCCCCCCCHHHHHHHCCEEC
VAGDQQAALFGQACFQPGDAKNTYGTGCFMLMNTGDRAVPSESGLLTTIAWGIGDRVEYA
CCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCEEEE
LEGSIFIGGAAVQWLRDELRFFDRAADSEALALSVPDSGGVYVVPAFVGLGAPYWDMYAR
ECCCEEECHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEHHHHCCCCHHHHHHC
GIVVGLTRGTGRAHITRATLESIAYQTRDVLGAMEKDSGIRLNRLKVDGGAVANNFLMQF
EEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHH
QSDILGVPVERPKIAETTALGAAYLAGLATGFWRSQAELADKWALDRGFTPALPAEERER
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LYAGWRRAVERARGWAAE
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA