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The map label for this gene is dacB [H]

Identifier: 51892127

GI number: 51892127

Start: 1106849

End: 1108546

Strand: Reverse

Name: dacB [H]

Synonym: STH989

Alternate gene names: 51892127

Gene position: 1108546-1106849 (Counterclockwise)

Preceding gene: 51892128

Following gene: 51892123

Centisome position: 31.09

GC content: 71.44

Gene sequence:

>1698_bases
GTGCAACCGCTCTCCCTTTATGTAAACGGACGGGAGCTTTATCTGCCACAGACCCCCATCCTCCAGGAAAACCGGGTCCT
GGTCCCCATGAGGGCCTACCTCGAATCGCTGGGCGCCGAGGTCGGATGGGAGCCGCCCAACCTGGTCACGGCCCGGATGG
GCGAACACACGGTGTCCCTCCGCATCGGCCAGTACACCGCGCAGGTGGACGGCCGTGAGGTGCCGCTGGACGTCCCGGCG
CAGATCATCGCCGACCGCACCTACGTCCCCCTCCGCTTCCTCTCCGAGGGGCTCGGAGCCGAGGTCGGGTACGACGGCGC
CACGCGCACCGTGACGGTGGTCACCGCACCTCCCGGGCAGCTGGAGGTCATCGACGGGCCCCTGAACGTGCGGGCGGAAC
CGAGCACCACCGCGCCCATCCTGACGACGGTGCCGGTGGGAACCCGGCTGGACATCGTGAGCGAGCAGCCGGGGGCGGAG
TGGACGCAGGTCGCCCTCCCGGGCGGCACACTCGGCTGGGTGGCGAACCGCTACACACGCACCGTGGGGAGCGAGCCGGT
GGTGCAGCCGCTGCTCGCGCTCCTGGAGCAGCGGGCCTACCTCCAGGCCGGCGGACAGTGCATCGGGGCCGTGCCGCTGG
TGAACAACCTGACGTACGTCCCGCTGGTGGAGGCGGTTGAGGCGCTGGGCGGTTCGGTCCGCCGGGCCGGTGACGGCACG
GCCATCGCCGCGGAGCTGGGCGGCCGGCAGCTTCTGATCACGCCCGACTCGGCGACCGCGACCCTGAACGGTCAGGCGGT
CGCGCTCTCCGCCGCCCCCCTGCTGGTCGGCGGCCGGCCGCTGATCCCCGCGCGCGACCTGGCGGACCACCTGGGGGTGA
CCCTCTCCTGGTCTGACGCCACCCGCACCGTGACCCTCGGCCCGGCCGGGGGCACCACCTGCATCCCCGACATCGCGGCG
CAGGCGTACATCCTGGTGGATGTGGCCACGGGAGCGGTGCTCAGCGAGTACAACGCCCGCGCCCCCCGGGCGATCGCCTC
GACCACCAAGATCATGACGGCGCTCCTGGCGGTGGAGCAGGGCCATCCCGACGCGGTGGTCACGGTGAGCGCCAACGCCG
CCAGCCAGCCGGGCACCAGCGTGTACCTGCGCGCCGGCGAGCAGCGCACGCTGCGGGAGCTGCTGTACGGGCTGATGCTG
GTCTCCGGCAACGACGCCGCCGTCGCCATCGCCGAGCACCTGGCCGGCAGCGAGGAGGCCTTCGCCCGGCGCATGAACCT
GCGGGCCGCGGAACTGGGCGCGCAGAACACGTATTTCGTCACGGCCTCCGGGCTCGACGACGACGTAAACCCCTACTCCA
CGGCGGAGGATCTGGCCCGCATCTCCCTGGCCTCCCTGCGCAACCCGCTGTTCCGCTCGTACATGTACCTGCCGCAGGCG
ACGATCCCCGGGCTGTGGGGCAACCGGCAGCTGACCAACAGCAACCTCTTCGTGCTCCGTTACCCCGGCGCCACAGGGGT
CAAGAACGGGTGGACGGAGAAGGCCGGGTACACCCTGGTGGCCTCGGCGTGGCGGGACGGCCGCGAGGTGATGCTGGTCC
TGCTGGGCGGCGAGAGCCGCAGCGCGCTCTACGCCGAGGCGTACAGTCTGATGGACCACGGCTTCGTCCTGGCAGACCAG
TCCTGGCTCCTCCGATGA

Upstream 100 bases:

>100_bases
GTTATTGAGGTGACACACCTTGGCAATACGCCGTAAGCTGGCTGCGGCCCTCGCCGCGACAACCCTGCTGGTCAGCGGGG
CACCGGCCCTCGCGGCCGAC

Downstream 100 bases:

>100_bases
GCGCGAAAAACGACCGCCCTCCGCAGCGCCGGAGGGCGGCTTCTGTTCGGCATTTCTCCATTCAGACCAGCTCGCGGGTG
GGAACGGCGAGGCGAGAGTC

Product: D-alanyl-D-alanine carboxypeptidase-like protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MQPLSLYVNGRELYLPQTPILQENRVLVPMRAYLESLGAEVGWEPPNLVTARMGEHTVSLRIGQYTAQVDGREVPLDVPA
QIIADRTYVPLRFLSEGLGAEVGYDGATRTVTVVTAPPGQLEVIDGPLNVRAEPSTTAPILTTVPVGTRLDIVSEQPGAE
WTQVALPGGTLGWVANRYTRTVGSEPVVQPLLALLEQRAYLQAGGQCIGAVPLVNNLTYVPLVEAVEALGGSVRRAGDGT
AIAAELGGRQLLITPDSATATLNGQAVALSAAPLLVGGRPLIPARDLADHLGVTLSWSDATRTVTLGPAGGTTCIPDIAA
QAYILVDVATGAVLSEYNARAPRAIASTTKIMTALLAVEQGHPDAVVTVSANAASQPGTSVYLRAGEQRTLRELLYGLML
VSGNDAAVAIAEHLAGSEEAFARRMNLRAAELGAQNTYFVTASGLDDDVNPYSTAEDLARISLASLRNPLFRSYMYLPQA
TIPGLWGNRQLTNSNLFVLRYPGATGVKNGWTEKAGYTLVASAWRDGREVMLVLLGGESRSALYAEAYSLMDHGFVLADQ
SWLLR

Sequences:

>Translated_565_residues
MQPLSLYVNGRELYLPQTPILQENRVLVPMRAYLESLGAEVGWEPPNLVTARMGEHTVSLRIGQYTAQVDGREVPLDVPA
QIIADRTYVPLRFLSEGLGAEVGYDGATRTVTVVTAPPGQLEVIDGPLNVRAEPSTTAPILTTVPVGTRLDIVSEQPGAE
WTQVALPGGTLGWVANRYTRTVGSEPVVQPLLALLEQRAYLQAGGQCIGAVPLVNNLTYVPLVEAVEALGGSVRRAGDGT
AIAAELGGRQLLITPDSATATLNGQAVALSAAPLLVGGRPLIPARDLADHLGVTLSWSDATRTVTLGPAGGTTCIPDIAA
QAYILVDVATGAVLSEYNARAPRAIASTTKIMTALLAVEQGHPDAVVTVSANAASQPGTSVYLRAGEQRTLRELLYGLML
VSGNDAAVAIAEHLAGSEEAFARRMNLRAAELGAQNTYFVTASGLDDDVNPYSTAEDLARISLASLRNPLFRSYMYLPQA
TIPGLWGNRQLTNSNLFVLRYPGATGVKNGWTEKAGYTLVASAWRDGREVMLVLLGGESRSALYAEAYSLMDHGFVLADQ
SWLLR
>Mature_565_residues
MQPLSLYVNGRELYLPQTPILQENRVLVPMRAYLESLGAEVGWEPPNLVTARMGEHTVSLRIGQYTAQVDGREVPLDVPA
QIIADRTYVPLRFLSEGLGAEVGYDGATRTVTVVTAPPGQLEVIDGPLNVRAEPSTTAPILTTVPVGTRLDIVSEQPGAE
WTQVALPGGTLGWVANRYTRTVGSEPVVQPLLALLEQRAYLQAGGQCIGAVPLVNNLTYVPLVEAVEALGGSVRRAGDGT
AIAAELGGRQLLITPDSATATLNGQAVALSAAPLLVGGRPLIPARDLADHLGVTLSWSDATRTVTLGPAGGTTCIPDIAA
QAYILVDVATGAVLSEYNARAPRAIASTTKIMTALLAVEQGHPDAVVTVSANAASQPGTSVYLRAGEQRTLRELLYGLML
VSGNDAAVAIAEHLAGSEEAFARRMNLRAAELGAQNTYFVTASGLDDDVNPYSTAEDLARISLASLRNPLFRSYMYLPQA
TIPGLWGNRQLTNSNLFVLRYPGATGVKNGWTEKAGYTLVASAWRDGREVMLVLLGGESRSALYAEAYSLMDHGFVLADQ
SWLLR

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=253, Percent_Identity=36.3636363636364, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1787062, Length=255, Percent_Identity=34.9019607843137, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI87082022, Length=251, Percent_Identity=33.0677290836653, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI87082054, Length=247, Percent_Identity=29.5546558704453, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 59942; Mature: 59942

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPLSLYVNGRELYLPQTPILQENRVLVPMRAYLESLGAEVGWEPPNLVTARMGEHTVSL
CCCEEEEECCEEEECCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEE
RIGQYTAQVDGREVPLDVPAQIIADRTYVPLRFLSEGLGAEVGYDGATRTVTVVTAPPGQ
EEEEEEEECCCCCCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCCCCCCEEEEEEECCCCC
LEVIDGPLNVRAEPSTTAPILTTVPVGTRLDIVSEQPGAEWTQVALPGGTLGWVANRYTR
EEEEECCCCEEECCCCCCCEEEEECCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHH
TVGSEPVVQPLLALLEQRAYLQAGGQCIGAVPLVNNLTYVPLVEAVEALGGSVRRAGDGT
HCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHCCEEHHHHHHHHHCCCCEEECCCCC
AIAAELGGRQLLITPDSATATLNGQAVALSAAPLLVGGRPLIPARDLADHLGVTLSWSDA
EEEEECCCCEEEEECCCCEEEECCCEEEEEECEEEECCCCCCCHHHHHHHCCEEEEECCC
TRTVTLGPAGGTTCIPDIAAQAYILVDVATGAVLSEYNARAPRAIASTTKIMTALLAVEQ
CEEEEECCCCCCCCCHHHHCCEEEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
GHPDAVVTVSANAASQPGTSVYLRAGEQRTLRELLYGLMLVSGNDAAVAIAEHLAGSEEA
CCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHEEEECCCCCEEHHHHHHCCCHHH
FARRMNLRAAELGAQNTYFVTASGLDDDVNPYSTAEDLARISLASLRNPLFRSYMYLPQA
HHHHHCCHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCC
TIPGLWGNRQLTNSNLFVLRYPGATGVKNGWTEKAGYTLVASAWRDGREVMLVLLGGESR
CCCCCCCCCEECCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCC
SALYAEAYSLMDHGFVLADQSWLLR
CHHHHHHHHHHHCCEEEECCHHHCC
>Mature Secondary Structure
MQPLSLYVNGRELYLPQTPILQENRVLVPMRAYLESLGAEVGWEPPNLVTARMGEHTVSL
CCCEEEEECCEEEECCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEE
RIGQYTAQVDGREVPLDVPAQIIADRTYVPLRFLSEGLGAEVGYDGATRTVTVVTAPPGQ
EEEEEEEECCCCCCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCCCCCCEEEEEEECCCCC
LEVIDGPLNVRAEPSTTAPILTTVPVGTRLDIVSEQPGAEWTQVALPGGTLGWVANRYTR
EEEEECCCCEEECCCCCCCEEEEECCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHH
TVGSEPVVQPLLALLEQRAYLQAGGQCIGAVPLVNNLTYVPLVEAVEALGGSVRRAGDGT
HCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHCCEEHHHHHHHHHCCCCEEECCCCC
AIAAELGGRQLLITPDSATATLNGQAVALSAAPLLVGGRPLIPARDLADHLGVTLSWSDA
EEEEECCCCEEEEECCCCEEEECCCEEEEEECEEEECCCCCCCHHHHHHHCCEEEEECCC
TRTVTLGPAGGTTCIPDIAAQAYILVDVATGAVLSEYNARAPRAIASTTKIMTALLAVEQ
CEEEEECCCCCCCCCHHHHCCEEEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
GHPDAVVTVSANAASQPGTSVYLRAGEQRTLRELLYGLMLVSGNDAAVAIAEHLAGSEEA
CCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHEEEECCCCCEEHHHHHHCCCHHH
FARRMNLRAAELGAQNTYFVTASGLDDDVNPYSTAEDLARISLASLRNPLFRSYMYLPQA
HHHHHCCHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCC
TIPGLWGNRQLTNSNLFVLRYPGATGVKNGWTEKAGYTLVASAWRDGREVMLVLLGGESR
CCCCCCCCCEECCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCC
SALYAEAYSLMDHGFVLADQSWLLR
CHHHHHHHHHHHCCEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]