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The map label for this gene is napA

Identifier: 51892055

GI number: 51892055

Start: 1036792

End: 1039056

Strand: Reverse

Name: napA

Synonym: STH917

Alternate gene names: 51892055

Gene position: 1039056-1036792 (Counterclockwise)

Preceding gene: 51892056

Following gene: 51892054

Centisome position: 29.14

GC content: 66.67

Gene sequence:

>2265_bases
ATGAAGTTCACCCGCCGTTCGTTCGTGAAGGCGTCGGCCCTCGCCACCGCCATGGTCGCGGCCGGATGCTCGCCGCAGCC
CGTCGCGCCCAAGCAGAACCCCGAGACGGAAGGCGCCACGTGGTACAAGACTGTCTGCCGCTACTGCGGCGTCGGTTGCG
GCGTGATGGTGGCTGCGAAGGACAACCGGGTGGTCGCGGTGAAGGGCGACACGGAGAACCCGGTCAACAAGGGACTTCTG
TGCGTCAAGGGCTATTATCTGGACCGGATCATGAACACCGAGGAAGGGCGGATCCTGAAGCCACTCATCCGGAAGAACGG
CCAGCTCACCGAAGCCTCCTGGGACGAGGCGCTCGACCTGGTGGCCGCCAGGTTCCGCGAGGCCATCGACCAGCACGGCC
CCGATTCGGTCGGCTTCTACGGATCCGGCCAGAACCTCGCCGAGGAGACCTACATCGCCAACAAGCTCTTCAAGGGCTGC
ATCGGCACCAACAACGTCGAGGGCAACCCGCGCACGTGCATGGCCTCCGCCGTGGCCGGCTTCCTCTCCACGTTCGGCAA
GGACGAGCCCATGGGCAACCTGGACGACATCGAGCATGCCGACACCTTCTTCATCATCGGCTCCAACACCGCGGAGGCCC
ATCCCATCATCTACTCCCGCATCACCACCCGCAAGCAGACGGGGAAGGACGTCAAGGTCATCCTGGCCGATCCGCGCAGG
CACCGGGTGGCCGACATCGCCGACATCTTCCTGCCCTTCCGGCCCGGCACCGACCTCGCCCTGCTGAACGCCTTCGCCCA
GGTGATCATCGAGGAAGGCCTCCACGACCCGGACTTCATCGAGCGTCACACCACGTTCCAGGATGGTAACGGCGCCATCA
CCTTCGAACAGTATGTCGCATTCCTGCAGGACTACACCCCGGAGAAGGTCGCCCCCATCACCGGCCTCTCGCCTGACGAC
ATCCGGGCGGCTGCCCGGGAGATCGGCGCCCGGGGCCGCAAGACCATGACGCTGTGGTGCATGGGCATCAACCAGCGCAC
GGTGGGCACGTGGCTGAACAACGCCATCTACAACCTGCACCTGCTGACGGGCAAGATCTGCCAGCCCGGCAACAGCCCCT
TCTCCCTCACCGGACAGCCCTCGGCCTGCGGCTCGGTGCGCGAGGGCGGCGGCCTGTCCCACCTGCTGCCCTGCGGCCGC
CAGGTGACCAACGAGCAGCACCGCAAGGAAGTGGCCGCGGTGTGGGGCGTCGACTATACCCGGATGTCGGACAAGGTCGG
TTACCACACCATGGAGATGTTCCGGGCCGCCGGCGACGGGCGCATCAAGGCCCTGCTGATCTCCTGCACCAACCCCGGCC
ACTCGCTGCCCAACCTGAACTCGGTGCGGGCCTCCCTGGAGAAGACCTTCCTCGTGGTCATGGACGCCTTCCACAACCGC
ACCACCGAGCTGGCGGACGTGGTGCTGCCCTCCGCCCTCTGGTGTGAGAAGGAGGGCATCTACGGCAACACCGAGCGCCG
GACCCAGCACCTGGCCAAGGCCGTCGAGCCCAAGGGTGAGGCCAGGCCCGACGTCTGGATCCTGCTGGAGATCGCCAAGC
GACTGGGCTACGGCGAGTACTTCAGCCACTACACCTCCAACGAGGTGATCTGGGAAGAGTTCCGGAAGATGGGCGGCGGC
GGCACCGGCTACGACTACGCGCCGTACGAGCGGTACAAGCAGGAGCGCGGCCTCCGCTGGCCGGTGAACGACAAGCAGCC
CGCCGGGACCACCCTGCGGTATGTGGAAGGCGACGACCCCTTCGTGCCGGAGGGCGCGGGGATCTACTTCTACGGCAAGC
CCGACGGCAAGGCGGTCATCTACGCCCGGCCCCACAGGGATCCGGCCGAGGTGCCCGACGCCGAGTACCCGTTTTACCTC
TCCACCGGGCGGATCCTCGAACACTGGCACACCATCACCATGACGAAGCGGGTGCCCGAGATCATGAAGGGCGCGGGCGA
GTTCTACTGCGAGATCCATGAGGAGGACGCCGCCCGGCTCGGCATCCAGAACGGCGACCTGGTGAAGCTGACCAGCCGCC
GCGGGCAGGTGGTCGCCACGGCGCGCGTCGGCGGCCGGGCCGTGCCCCAGAAGGGGCTGGTGTTCCTGCTCATGCACGAC
GACCGCACCGAGCGGCTCGCCAACTTCCTGACCAACGACGCGGTCGACGAGACCTCCAAGCAGATGGAGTACAAGGTCTG
CGCCGTGCGGGTTGAGAAGGCGTAG

Upstream 100 bases:

>100_bases
GCACGGAAGCGTCGGTCAGGACACTTGACCACCTGAGGGCCATTCCCGGCGTCTACTCGGTCCTGCCGGCGTACATTCAA
TCCGTTGAGGAGGATCGGAC

Downstream 100 bases:

>100_bases
GCGGTGCGGGCGATGATGAAGAAGATCGGGTGGCGCAGGCTGCGCCTCGCCACCCAGCTCCTTTTCTGGGCGCTCCTCCT
GCTGCCGCTCGCGGGCTTCC

Product: nitrate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 754; Mature: 754

Protein sequence:

>754_residues
MKFTRRSFVKASALATAMVAAGCSPQPVAPKQNPETEGATWYKTVCRYCGVGCGVMVAAKDNRVVAVKGDTENPVNKGLL
CVKGYYLDRIMNTEEGRILKPLIRKNGQLTEASWDEALDLVAARFREAIDQHGPDSVGFYGSGQNLAEETYIANKLFKGC
IGTNNVEGNPRTCMASAVAGFLSTFGKDEPMGNLDDIEHADTFFIIGSNTAEAHPIIYSRITTRKQTGKDVKVILADPRR
HRVADIADIFLPFRPGTDLALLNAFAQVIIEEGLHDPDFIERHTTFQDGNGAITFEQYVAFLQDYTPEKVAPITGLSPDD
IRAAAREIGARGRKTMTLWCMGINQRTVGTWLNNAIYNLHLLTGKICQPGNSPFSLTGQPSACGSVREGGGLSHLLPCGR
QVTNEQHRKEVAAVWGVDYTRMSDKVGYHTMEMFRAAGDGRIKALLISCTNPGHSLPNLNSVRASLEKTFLVVMDAFHNR
TTELADVVLPSALWCEKEGIYGNTERRTQHLAKAVEPKGEARPDVWILLEIAKRLGYGEYFSHYTSNEVIWEEFRKMGGG
GTGYDYAPYERYKQERGLRWPVNDKQPAGTTLRYVEGDDPFVPEGAGIYFYGKPDGKAVIYARPHRDPAEVPDAEYPFYL
STGRILEHWHTITMTKRVPEIMKGAGEFYCEIHEEDAARLGIQNGDLVKLTSRRGQVVATARVGGRAVPQKGLVFLLMHD
DRTERLANFLTNDAVDETSKQMEYKVCAVRVEKA

Sequences:

>Translated_754_residues
MKFTRRSFVKASALATAMVAAGCSPQPVAPKQNPETEGATWYKTVCRYCGVGCGVMVAAKDNRVVAVKGDTENPVNKGLL
CVKGYYLDRIMNTEEGRILKPLIRKNGQLTEASWDEALDLVAARFREAIDQHGPDSVGFYGSGQNLAEETYIANKLFKGC
IGTNNVEGNPRTCMASAVAGFLSTFGKDEPMGNLDDIEHADTFFIIGSNTAEAHPIIYSRITTRKQTGKDVKVILADPRR
HRVADIADIFLPFRPGTDLALLNAFAQVIIEEGLHDPDFIERHTTFQDGNGAITFEQYVAFLQDYTPEKVAPITGLSPDD
IRAAAREIGARGRKTMTLWCMGINQRTVGTWLNNAIYNLHLLTGKICQPGNSPFSLTGQPSACGSVREGGGLSHLLPCGR
QVTNEQHRKEVAAVWGVDYTRMSDKVGYHTMEMFRAAGDGRIKALLISCTNPGHSLPNLNSVRASLEKTFLVVMDAFHNR
TTELADVVLPSALWCEKEGIYGNTERRTQHLAKAVEPKGEARPDVWILLEIAKRLGYGEYFSHYTSNEVIWEEFRKMGGG
GTGYDYAPYERYKQERGLRWPVNDKQPAGTTLRYVEGDDPFVPEGAGIYFYGKPDGKAVIYARPHRDPAEVPDAEYPFYL
STGRILEHWHTITMTKRVPEIMKGAGEFYCEIHEEDAARLGIQNGDLVKLTSRRGQVVATARVGGRAVPQKGLVFLLMHD
DRTERLANFLTNDAVDETSKQMEYKVCAVRVEKA
>Mature_754_residues
MKFTRRSFVKASALATAMVAAGCSPQPVAPKQNPETEGATWYKTVCRYCGVGCGVMVAAKDNRVVAVKGDTENPVNKGLL
CVKGYYLDRIMNTEEGRILKPLIRKNGQLTEASWDEALDLVAARFREAIDQHGPDSVGFYGSGQNLAEETYIANKLFKGC
IGTNNVEGNPRTCMASAVAGFLSTFGKDEPMGNLDDIEHADTFFIIGSNTAEAHPIIYSRITTRKQTGKDVKVILADPRR
HRVADIADIFLPFRPGTDLALLNAFAQVIIEEGLHDPDFIERHTTFQDGNGAITFEQYVAFLQDYTPEKVAPITGLSPDD
IRAAAREIGARGRKTMTLWCMGINQRTVGTWLNNAIYNLHLLTGKICQPGNSPFSLTGQPSACGSVREGGGLSHLLPCGR
QVTNEQHRKEVAAVWGVDYTRMSDKVGYHTMEMFRAAGDGRIKALLISCTNPGHSLPNLNSVRASLEKTFLVVMDAFHNR
TTELADVVLPSALWCEKEGIYGNTERRTQHLAKAVEPKGEARPDVWILLEIAKRLGYGEYFSHYTSNEVIWEEFRKMGGG
GTGYDYAPYERYKQERGLRWPVNDKQPAGTTLRYVEGDDPFVPEGAGIYFYGKPDGKAVIYARPHRDPAEVPDAEYPFYL
STGRILEHWHTITMTKRVPEIMKGAGEFYCEIHEEDAARLGIQNGDLVKLTSRRGQVVATARVGGRAVPQKGLVFLLMHD
DRTERLANFLTNDAVDETSKQMEYKVCAVRVEKA

Specific function: Catalytic subunit of the nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC. Essential function for nitrate assimilation and may have a role in a

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted (Potential). Note=Membrane- associated (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily

Homologues:

Organism=Escherichia coli, GI1788534, Length=833, Percent_Identity=41.296518607443, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI3868721, Length=719, Percent_Identity=33.240611961057, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI3868719, Length=664, Percent_Identity=25.1506024096386, Blast_Score=150, Evalue=4e-37,
Organism=Escherichia coli, GI1787870, Length=783, Percent_Identity=25.5427841634738, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI3868720, Length=668, Percent_Identity=24.7005988023952, Blast_Score=143, Evalue=4e-35,
Organism=Escherichia coli, GI171474008, Length=781, Percent_Identity=24.583866837388, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI87081994, Length=789, Percent_Identity=23.8276299112801, Blast_Score=113, Evalue=6e-26,
Organism=Escherichia coli, GI87081797, Length=809, Percent_Identity=23.6093943139679, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1787778, Length=321, Percent_Identity=25.5451713395639, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAPA_SYMTH (Q67QZ1)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_074746.1
- ProteinModelPortal:   Q67QZ1
- GeneID:   2979700
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH917
- NMPDR:   fig|292459.1.peg.881
- HOGENOM:   HBG715033
- OMA:   IISAPYE
- ProtClustDB:   CLSK748640
- BioCyc:   STHE292459:STH917-MONOMER
- BRENDA:   1.7.99.4
- GO:   GO:0006810
- HAMAP:   MF_01630
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311
- InterPro:   IPR019546
- Gene3D:   G3DSA:2.40.40.20
- SMART:   SM00926
- TIGRFAMs:   TIGR01409

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding; PF10518 TAT_signal; SSF50692 Asp_decarb_fold

EC number: =1.7.99.4

Molecular weight: Translated: 83581; Mature: 83581

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1; PS00490 MOLYBDOPTERIN_PROK_2; PS00932 MOLYBDOPTERIN_PROK_3; PS51318 TAT; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTRRSFVKASALATAMVAAGCSPQPVAPKQNPETEGATWYKTVCRYCGVGCGVMVAAK
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEC
DNRVVAVKGDTENPVNKGLLCVKGYYLDRIMNTEEGRILKPLIRKNGQLTEASWDEALDL
CCEEEEEECCCCCCHHCCEEEEEHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHHHH
VAARFREAIDQHGPDSVGFYGSGQNLAEETYIANKLFKGCIGTNNVEGNPRTCMASAVAG
HHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
FLSTFGKDEPMGNLDDIEHADTFFIIGSNTAEAHPIIYSRITTRKQTGKDVKVILADPRR
HHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEHHHHHHHCCCCCEEEEEECCCH
HRVADIADIFLPFRPGTDLALLNAFAQVIIEEGLHDPDFIERHTTFQDGNGAITFEQYVA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCEECCCCCEEHHHHHH
FLQDYTPEKVAPITGLSPDDIRAAAREIGARGRKTMTLWCMGINQRTVGTWLNNAIYNLH
HHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHEEEEE
LLTGKICQPGNSPFSLTGQPSACGSVREGGGLSHLLPCGRQVTNEQHRKEVAAVWGVDYT
EEECCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHCCHHHCCHHHHHHHHHHHCCCHH
RMSDKVGYHTMEMFRAAGDGRIKALLISCTNPGHSLPNLNSVRASLEKTFLVVMDAFHNR
HHHHHCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
TTELADVVLPSALWCEKEGIYGNTERRTQHLAKAVEPKGEARPDVWILLEIAKRLGYGEY
HHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHH
FSHYTSNEVIWEEFRKMGGGGTGYDYAPYERYKQERGLRWPVNDKQPAGTTLRYVEGDDP
HHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCC
FVPEGAGIYFYGKPDGKAVIYARPHRDPAEVPDAEYPFYLSTGRILEHWHTITMTKRVPE
CCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHH
IMKGAGEFYCEIHEEDAARLGIQNGDLVKLTSRRGQVVATARVGGRAVPQKGLVFLLMHD
HHHCCCCEEEEEECCCHHHCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCEEEEEEEC
DRTERLANFLTNDAVDETSKQMEYKVCAVRVEKA
CHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECC
>Mature Secondary Structure
MKFTRRSFVKASALATAMVAAGCSPQPVAPKQNPETEGATWYKTVCRYCGVGCGVMVAAK
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEC
DNRVVAVKGDTENPVNKGLLCVKGYYLDRIMNTEEGRILKPLIRKNGQLTEASWDEALDL
CCEEEEEECCCCCCHHCCEEEEEHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHHHH
VAARFREAIDQHGPDSVGFYGSGQNLAEETYIANKLFKGCIGTNNVEGNPRTCMASAVAG
HHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
FLSTFGKDEPMGNLDDIEHADTFFIIGSNTAEAHPIIYSRITTRKQTGKDVKVILADPRR
HHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEHHHHHHHCCCCCEEEEEECCCH
HRVADIADIFLPFRPGTDLALLNAFAQVIIEEGLHDPDFIERHTTFQDGNGAITFEQYVA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCEECCCCCEEHHHHHH
FLQDYTPEKVAPITGLSPDDIRAAAREIGARGRKTMTLWCMGINQRTVGTWLNNAIYNLH
HHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHEEEEE
LLTGKICQPGNSPFSLTGQPSACGSVREGGGLSHLLPCGRQVTNEQHRKEVAAVWGVDYT
EEECCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHCCHHHCCHHHHHHHHHHHCCCHH
RMSDKVGYHTMEMFRAAGDGRIKALLISCTNPGHSLPNLNSVRASLEKTFLVVMDAFHNR
HHHHHCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
TTELADVVLPSALWCEKEGIYGNTERRTQHLAKAVEPKGEARPDVWILLEIAKRLGYGEY
HHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHH
FSHYTSNEVIWEEFRKMGGGGTGYDYAPYERYKQERGLRWPVNDKQPAGTTLRYVEGDDP
HHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCC
FVPEGAGIYFYGKPDGKAVIYARPHRDPAEVPDAEYPFYLSTGRILEHWHTITMTKRVPE
CCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHH
IMKGAGEFYCEIHEEDAARLGIQNGDLVKLTSRRGQVVATARVGGRAVPQKGLVFLLMHD
HHHCCCCEEEEEECCCHHHCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCEEEEEEEC
DRTERLANFLTNDAVDETSKQMEYKVCAVRVEKA
CHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA