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The map label for this gene is livJ [C]

Identifier: 51891941

GI number: 51891941

Start: 899898

End: 901184

Strand: Reverse

Name: livJ [C]

Synonym: STH803

Alternate gene names: 51891941

Gene position: 901184-899898 (Counterclockwise)

Preceding gene: 51891942

Following gene: 51891940

Centisome position: 25.27

GC content: 67.37

Gene sequence:

>1287_bases
GTGAGACGTTGGTTCACTGCGATCCTCGCCTGCGCGCTTTCCCTGGCCCTGATCGCCGGGTGCTCGGCGCCGCCCGAGGC
GGAGTCGCCGGGGCAGACCTCCGGCCCGTCCTCCAGCCAGCCGGCCGGGCAGACGGGCGGACAGTCCGGGGAGACCCAGC
CCCGGCGTGAGGTCCAGGGCGTCACCGACACCACCGTCAAGGTCGGCGGCTTCGCCGCCCAGTCCGGCCCCGTGGCGCCG
ATCGGCATCTCGGTGCGCAAGGGCTTCGAGGCCTACATCAAGCACGTCAACGACCAGGGCGGCGTCCACGGCCGGAAGAT
CGAGATCGTGAACATCATCGACGACCAGTTCGACGGCTCCAAGGCCCTGACGGCCGCCAAGCAGCTGGTGGAGTCGGACA
AGGTCTTCGCCCTGGCGCCCATCCTGGGCACCGCCGGCTTCATGGCCAGCTACGACTACTTCGTGGAGAAGCGGATCCCC
GTCATCTACCCGATGACCGGCGTGGAACAGACGGCTCACCCGCCCCAGGAGAACATCTTCGCGGTGCAGCCCAACAACTA
CGACGAGGCCAAGATCCTCACCCAGTACCTGGTGGACGAGCTGGGCCTGAAGAAGATCGCCGTGCTCTGGCAGAACAGCG
ACATGGGTGAGCAGGGCCTGAAGGGCGTGAAGGACTACATCGGCGAGCTCGGGGCTGAACTGGTCTACGACGCCCCGCAC
GACGCCGGCTCGGTGGACTTCACCACCCCGGTGCTCAACCTGAAGCAGAAGGGCGCCGAGGGCGTGGTGATCATGACCAC
CCTGGCGGAGACCATCGGCATCGTGAAGGAGATGCACAAGCAAGGCGTCACCGCCGTGCCCATCACCTCCTACGTCAACG
CCGACCCGGTGAACGTGCCGGCCCTCGCGGGCGAGGCGGCCATCGGCCTCTACGCCGGCGGCTGGGTGCCCGTGGCCGAC
CCCGACGACCCCAACATCCAGAAGTTCTGGGAGATCTACGGCCGGTACAACAACGGCGAGCTGCCTGACGCCTACGGCAC
CGCCGGCTTCATCGCCGCCGAGCTGCTGGTGAAGGGCCTGGAGGACGCCGGCCGCGACCTCACCTACGAGAGCTTCATCC
AGGCGCTCGAGAACCTGAACGACTGGAGCGGCATCATGGCCCGGAACCTGACCTACGGCCCGGGCGAGCGGGCGGGCGTC
AAGGCCTTCTACCTGAACAAGGTCATCGAGCAGGACGGCAACATCGTGATGGTGCAGGTGAACCAGGAGCCGTACCGCCT
CCGGTAA

Upstream 100 bases:

>100_bases
GCATCGGCGGCGGCCAGGGCATCGCCATGGTGGTCGAGCGGGCCTGACCGGTCCGCCTGCACCCTGCACGCATCTCCATC
ACAGATAGGGGGAGACAGAC

Downstream 100 bases:

>100_bases
ACCGAAAGCCCACACCGCGCTCCGCCGGGCCGCACCAGCCGGGTCCGCACGGGTCCTCAGGCGGCCCCGGCGGGAGCGCA
CCCACCACCGCTTCCTGACA

Product: putative branched chain amino acid ABC transporter substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MRRWFTAILACALSLALIAGCSAPPEAESPGQTSGPSSSQPAGQTGGQSGETQPRREVQGVTDTTVKVGGFAAQSGPVAP
IGISVRKGFEAYIKHVNDQGGVHGRKIEIVNIIDDQFDGSKALTAAKQLVESDKVFALAPILGTAGFMASYDYFVEKRIP
VIYPMTGVEQTAHPPQENIFAVQPNNYDEAKILTQYLVDELGLKKIAVLWQNSDMGEQGLKGVKDYIGELGAELVYDAPH
DAGSVDFTTPVLNLKQKGAEGVVIMTTLAETIGIVKEMHKQGVTAVPITSYVNADPVNVPALAGEAAIGLYAGGWVPVAD
PDDPNIQKFWEIYGRYNNGELPDAYGTAGFIAAELLVKGLEDAGRDLTYESFIQALENLNDWSGIMARNLTYGPGERAGV
KAFYLNKVIEQDGNIVMVQVNQEPYRLR

Sequences:

>Translated_428_residues
MRRWFTAILACALSLALIAGCSAPPEAESPGQTSGPSSSQPAGQTGGQSGETQPRREVQGVTDTTVKVGGFAAQSGPVAP
IGISVRKGFEAYIKHVNDQGGVHGRKIEIVNIIDDQFDGSKALTAAKQLVESDKVFALAPILGTAGFMASYDYFVEKRIP
VIYPMTGVEQTAHPPQENIFAVQPNNYDEAKILTQYLVDELGLKKIAVLWQNSDMGEQGLKGVKDYIGELGAELVYDAPH
DAGSVDFTTPVLNLKQKGAEGVVIMTTLAETIGIVKEMHKQGVTAVPITSYVNADPVNVPALAGEAAIGLYAGGWVPVAD
PDDPNIQKFWEIYGRYNNGELPDAYGTAGFIAAELLVKGLEDAGRDLTYESFIQALENLNDWSGIMARNLTYGPGERAGV
KAFYLNKVIEQDGNIVMVQVNQEPYRLR
>Mature_428_residues
MRRWFTAILACALSLALIAGCSAPPEAESPGQTSGPSSSQPAGQTGGQSGETQPRREVQGVTDTTVKVGGFAAQSGPVAP
IGISVRKGFEAYIKHVNDQGGVHGRKIEIVNIIDDQFDGSKALTAAKQLVESDKVFALAPILGTAGFMASYDYFVEKRIP
VIYPMTGVEQTAHPPQENIFAVQPNNYDEAKILTQYLVDELGLKKIAVLWQNSDMGEQGLKGVKDYIGELGAELVYDAPH
DAGSVDFTTPVLNLKQKGAEGVVIMTTLAETIGIVKEMHKQGVTAVPITSYVNADPVNVPALAGEAAIGLYAGGWVPVAD
PDDPNIQKFWEIYGRYNNGELPDAYGTAGFIAAELLVKGLEDAGRDLTYESFIQALENLNDWSGIMARNLTYGPGERAGV
KAFYLNKVIEQDGNIVMVQVNQEPYRLR

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=248, Percent_Identity=24.5967741935484, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 45950; Mature: 45950

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRWFTAILACALSLALIAGCSAPPEAESPGQTSGPSSSQPAGQTGGQSGETQPRREVQG
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
VTDTTVKVGGFAAQSGPVAPIGISVRKGFEAYIKHVNDQGGVHGRKIEIVNIIDDQFDGS
CCCCEEEECCEECCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCH
KALTAAKQLVESDKVFALAPILGTAGFMASYDYFVEKRIPVIYPMTGVEQTAHPPQENIF
HHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEE
AVQPNNYDEAKILTQYLVDELGLKKIAVLWQNSDMGEQGLKGVKDYIGELGAELVYDAPH
EECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCC
DAGSVDFTTPVLNLKQKGAEGVVIMTTLAETIGIVKEMHKQGVTAVPITSYVNADPVNVP
CCCCCEECCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEEHHCCCCCCCCCC
ALAGEAAIGLYAGGWVPVADPDDPNIQKFWEIYGRYNNGELPDAYGTAGFIAAELLVKGL
CCCCCCEEEEEECCEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
EDAGRDLTYESFIQALENLNDWSGIMARNLTYGPGERAGVKAFYLNKVIEQDGNIVMVQV
HHCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEE
NQEPYRLR
CCCCCCCC
>Mature Secondary Structure
MRRWFTAILACALSLALIAGCSAPPEAESPGQTSGPSSSQPAGQTGGQSGETQPRREVQG
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
VTDTTVKVGGFAAQSGPVAPIGISVRKGFEAYIKHVNDQGGVHGRKIEIVNIIDDQFDGS
CCCCEEEECCEECCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCH
KALTAAKQLVESDKVFALAPILGTAGFMASYDYFVEKRIPVIYPMTGVEQTAHPPQENIF
HHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEE
AVQPNNYDEAKILTQYLVDELGLKKIAVLWQNSDMGEQGLKGVKDYIGELGAELVYDAPH
EECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCC
DAGSVDFTTPVLNLKQKGAEGVVIMTTLAETIGIVKEMHKQGVTAVPITSYVNADPVNVP
CCCCCEECCHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEEHHCCCCCCCCCC
ALAGEAAIGLYAGGWVPVADPDDPNIQKFWEIYGRYNNGELPDAYGTAGFIAAELLVKGL
CCCCCCEEEEEECCEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
EDAGRDLTYESFIQALENLNDWSGIMARNLTYGPGERAGVKAFYLNKVIEQDGNIVMVQV
HHCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEE
NQEPYRLR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA