BacMap

Definition	Symbiobacterium thermophilum IAM 14863 chromosome, complete genome.
Accession	NC_006177
Length	3,566,135

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The map label for this gene is braF [H]

Identifier: 51891938

GI number: 51891938

Start: 897038

End: 897832

Strand: Reverse

Name: braF [H]

Synonym: STH800

Alternate gene names: 51891938

Gene position: 897832-897038 (Counterclockwise)

Preceding gene: 51891939

Following gene: 51891937

Centisome position: 25.18

GC content: 69.18

Gene sequence:

>795_bases
ATGGCCCTGCTGGAGCTCAGGCAGGTGACGATGCGCTTCGGCGGCATCGTCGCGGTGGACCGGCTGGACCTCGCGGTGGA
GGAAGGGACCATCCACGGGCTCATCGGCCCCAACGGCGCCGGCAAGTCCACCGTCTTCAACCTCATCAGCCGGATCTACG
ACCCCGTGGGCGGCAGCATCGCCTTCGCGGGAAAGGACCTGCTGCGGATGGCGCCCCACGACGTCATCCATCACGGCATC
GCCCGCACCTTCCAGAACGTGGAGCTCTTCCGCGGCCTGACGGTGCTGGGCAACCTGCTGGTCGGCCAGCACAGCCGCAT
CCGCTACGGCCTCCTCGCCTCGGCCCTGGGGAACCTGGTCCCCGCGGTCCGTCGGGAGGAGCGTGAGGCGGAGGAGCGAG
CCCTGGAGGTGCTGGAGTTCCTGGGCATCGCCGCCTACAAGGACGCCTACCCGCAGCTGCTGCCCTACGGCATCCAGAAG
CGGGTGGAGTTCGGCCGGGCGCTGGTGGCGCAGCCGCGGCTGATCCTCCTGGACGAGCCGGCCGCCGGCATGAACCCGCA
GGAGACGCTGGAACTGGCGGAGCTGATCCGGGAGGTCCGGGACCGGTACAGGGCCACGGTGCTCCTGGTGGAGCACGACA
TGAGCCTGGTAATGCGCATCTGCGAGCGGATCACGGTGATGGATTTCGGCCGGAAGATCGCCGAAGGCACCCCCGCCGAG
GTGCAGTCCGACCCGGCGGTGATCCGGGCCTACCTGGGCGAGGAGGACGATGAGGAGGGTGCCGCTCATGCTTGA

Upstream 100 bases:

>100_bases
GGTGGTGCTCTACCTGCCCCTGGGGCTCGTGGACCTGTGGCGGAGGCTGCAGCACTGGTGGGCCGGGCGCCGGCACGCCG
CGGCCGGGGGGGTGGAGTAG

Downstream 100 bases:

>100_bases
GCTGCAAGGCGTAAGCGCCGCCTACGGCAAGATCCGGGCCCTCTCCGACCTCACCCTCACGGTGCCGGACGGGCAGATCG
TCACCCTGCTCGGGGCCAAC

Product: branched-chain amino acid ABC transporter ATP-binding protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MALLELRQVTMRFGGIVAVDRLDLAVEEGTIHGLIGPNGAGKSTVFNLISRIYDPVGGSIAFAGKDLLRMAPHDVIHHGI
ARTFQNVELFRGLTVLGNLLVGQHSRIRYGLLASALGNLVPAVRREEREAEERALEVLEFLGIAAYKDAYPQLLPYGIQK
RVEFGRALVAQPRLILLDEPAAGMNPQETLELAELIREVRDRYRATVLLVEHDMSLVMRICERITVMDFGRKIAEGTPAE
VQSDPAVIRAYLGEEDDEEGAAHA

Sequences:

>Translated_264_residues
MALLELRQVTMRFGGIVAVDRLDLAVEEGTIHGLIGPNGAGKSTVFNLISRIYDPVGGSIAFAGKDLLRMAPHDVIHHGI
ARTFQNVELFRGLTVLGNLLVGQHSRIRYGLLASALGNLVPAVRREEREAEERALEVLEFLGIAAYKDAYPQLLPYGIQK
RVEFGRALVAQPRLILLDEPAAGMNPQETLELAELIREVRDRYRATVLLVEHDMSLVMRICERITVMDFGRKIAEGTPAE
VQSDPAVIRAYLGEEDDEEGAAHA
>Mature_263_residues
ALLELRQVTMRFGGIVAVDRLDLAVEEGTIHGLIGPNGAGKSTVFNLISRIYDPVGGSIAFAGKDLLRMAPHDVIHHGIA
RTFQNVELFRGLTVLGNLLVGQHSRIRYGLLASALGNLVPAVRREEREAEERALEVLEFLGIAAYKDAYPQLLPYGIQKR
VEFGRALVAQPRLILLDEPAAGMNPQETLELAELIREVRDRYRATVLLVEHDMSLVMRICERITVMDFGRKIAEGTPAEV
QSDPAVIRAYLGEEDDEEGAAHA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI105990541, Length=243, Percent_Identity=30.0411522633745, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI27881501, Length=233, Percent_Identity=27.0386266094421, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI30795238, Length=233, Percent_Identity=27.0386266094421, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI45446740, Length=222, Percent_Identity=29.2792792792793, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI47078218, Length=222, Percent_Identity=29.2792792792793, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI156105685, Length=236, Percent_Identity=29.2372881355932, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI21536376, Length=227, Percent_Identity=27.7533039647577, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI150417984, Length=250, Percent_Identity=29.6, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI31657092, Length=255, Percent_Identity=26.2745098039216, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI27477115, Length=173, Percent_Identity=27.7456647398844, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI153792144, Length=216, Percent_Identity=25.9259259259259, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI116734710, Length=239, Percent_Identity=25.9414225941423, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI9665248, Length=208, Percent_Identity=27.8846153846154, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI27436953, Length=226, Percent_Identity=26.9911504424779, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI6005701, Length=234, Percent_Identity=24.7863247863248, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI42741659, Length=228, Percent_Identity=28.5087719298246, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI21536378, Length=241, Percent_Identity=23.6514522821577, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1789864, Length=252, Percent_Identity=46.031746031746, Blast_Score=230, Evalue=6e-62,
Organism=Escherichia coli, GI1789593, Length=255, Percent_Identity=35.2941176470588, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1789032, Length=246, Percent_Identity=30.0813008130081, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1786398, Length=249, Percent_Identity=30.1204819277108, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788225, Length=259, Percent_Identity=29.3436293436293, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI87081709, Length=256, Percent_Identity=28.125, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1788450, Length=258, Percent_Identity=28.2945736434109, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788761, Length=263, Percent_Identity=28.1368821292776, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI48994943, Length=239, Percent_Identity=29.2887029288703, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87081782, Length=260, Percent_Identity=28.8461538461538, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1790525, Length=244, Percent_Identity=28.2786885245902, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI87081791, Length=245, Percent_Identity=28.5714285714286, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI48995001, Length=221, Percent_Identity=30.7692307692308, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789991, Length=256, Percent_Identity=30.859375, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787029, Length=251, Percent_Identity=27.0916334661355, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790467, Length=247, Percent_Identity=29.1497975708502, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87082267, Length=249, Percent_Identity=30.1204819277108, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI1790190, Length=246, Percent_Identity=27.6422764227642, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1787712, Length=228, Percent_Identity=27.6315789473684, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789672, Length=264, Percent_Identity=27.2727272727273, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788210, Length=252, Percent_Identity=27.7777777777778, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787370, Length=254, Percent_Identity=27.1653543307087, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788472, Length=232, Percent_Identity=25.8620689655172, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1787548, Length=276, Percent_Identity=26.4492753623188, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786872, Length=256, Percent_Identity=26.953125, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787089, Length=238, Percent_Identity=27.3109243697479, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786253, Length=233, Percent_Identity=30.4721030042918, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1786319, Length=228, Percent_Identity=27.1929824561404, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1789962, Length=231, Percent_Identity=24.6753246753247, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1790544, Length=256, Percent_Identity=26.953125, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082268, Length=252, Percent_Identity=27.3809523809524, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786803, Length=251, Percent_Identity=25.4980079681275, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787500, Length=231, Percent_Identity=26.8398268398268, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1789963, Length=250, Percent_Identity=26.8, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87081834, Length=244, Percent_Identity=27.0491803278689, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787792, Length=247, Percent_Identity=26.3157894736842, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787759, Length=257, Percent_Identity=27.6264591439689, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI48994883, Length=254, Percent_Identity=24.0157480314961, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1790739, Length=253, Percent_Identity=26.0869565217391, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786345, Length=236, Percent_Identity=25.8474576271186, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1789586, Length=247, Percent_Identity=25.9109311740891, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788897, Length=227, Percent_Identity=27.3127753303965, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788506, Length=234, Percent_Identity=25.2136752136752, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI1789873, Length=220, Percent_Identity=29.0909090909091, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786563, Length=261, Percent_Identity=27.2030651340996, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786703, Length=208, Percent_Identity=27.8846153846154, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787164, Length=228, Percent_Identity=27.1929824561404, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1790162, Length=261, Percent_Identity=22.6053639846743, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI48994997, Length=248, Percent_Identity=25.8064516129032, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789891, Length=216, Percent_Identity=25.9259259259259, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1787758, Length=258, Percent_Identity=23.6434108527132, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787105, Length=199, Percent_Identity=28.1407035175879, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788644, Length=221, Percent_Identity=23.9819004524887, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787041, Length=240, Percent_Identity=27.0833333333333, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787182, Length=243, Percent_Identity=25.1028806584362, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693107, Length=232, Percent_Identity=24.1379310344828, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787112, Length=225, Percent_Identity=28.8888888888889, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787143, Length=241, Percent_Identity=25.3112033195021, Blast_Score=61, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI71983523, Length=240, Percent_Identity=30, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71983516, Length=240, Percent_Identity=30, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71983510, Length=240, Percent_Identity=30, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI115533608, Length=223, Percent_Identity=30.4932735426009, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17565938, Length=228, Percent_Identity=28.0701754385965, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17565586, Length=221, Percent_Identity=27.6018099547511, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17541710, Length=230, Percent_Identity=28.2608695652174, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI115534520, Length=232, Percent_Identity=23.7068965517241, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI193202349, Length=229, Percent_Identity=27.0742358078603, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17543740, Length=214, Percent_Identity=26.6355140186916, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI115533592, Length=240, Percent_Identity=26.6666666666667, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24659289, Length=248, Percent_Identity=29.8387096774194, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24643674, Length=234, Percent_Identity=23.9316239316239, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI281363262, Length=232, Percent_Identity=26.7241379310345, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI85726357, Length=272, Percent_Identity=25.7352941176471, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24653245, Length=234, Percent_Identity=26.4957264957265, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI23397593, Length=232, Percent_Identity=25.8620689655172, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17136662, Length=224, Percent_Identity=25, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24666092, Length=231, Percent_Identity=24.6753246753247, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 29113; Mature: 28982

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLELRQVTMRFGGIVAVDRLDLAVEEGTIHGLIGPNGAGKSTVFNLISRIYDPVGGSI
CCHHHHHHHHHHHCCEEEEHHHHHEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCE
AFAGKDLLRMAPHDVIHHGIARTFQNVELFRGLTVLGNLLVGQHSRIRYGLLASALGNLV
EEHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
PAVRREEREAEERALEVLEFLGIAAYKDAYPQLLPYGIQKRVEFGRALVAQPRLILLDEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
AAGMNPQETLELAELIREVRDRYRATVLLVEHDMSLVMRICERITVMDFGRKIAEGTPAE
CCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCC
VQSDPAVIRAYLGEEDDEEGAAHA
CCCCHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ALLELRQVTMRFGGIVAVDRLDLAVEEGTIHGLIGPNGAGKSTVFNLISRIYDPVGGSI
CHHHHHHHHHHHCCEEEEHHHHHEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCE
AFAGKDLLRMAPHDVIHHGIARTFQNVELFRGLTVLGNLLVGQHSRIRYGLLASALGNLV
EEHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
PAVRREEREAEERALEVLEFLGIAAYKDAYPQLLPYGIQKRVEFGRALVAQPRLILLDEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
AAGMNPQETLELAELIREVRDRYRATVLLVEHDMSLVMRICERITVMDFGRKIAEGTPAE
CCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCC
VQSDPAVIRAYLGEEDDEEGAAHA
CCCCHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]