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The map label for this gene is metK [H]

Identifier: 51598632

GI number: 51598632

Start: 384919

End: 386097

Strand: Direct

Name: metK [H]

Synonym: BG0375

Alternate gene names: 51598632

Gene position: 384919-386097 (Clockwise)

Preceding gene: 51598631

Following gene: 51598633

Centisome position: 42.57

GC content: 32.23

Gene sequence:

>1179_bases
ATGAATAAAATACTAGCAGCTAACCAAACTTTAACTTCTGAGGCTGTATCTGAAGGACATCCAGATAAAATTGCAGATCA
AATCTCTGATGCAATCCTTGATGAAATACTAAAAGTAGATAAAAATGCAAAAGTAGCTTGCGAAGTCATAATTACACAAA
ATTTAGTAGTAATAGCAGGAGAAATAAATAGTCCTGTTAAAAAAACCTTAGATATAAAAGAAATTGCTAAAAAAATCATT
AAAGATATAGGCTATACAAATATTGATTATGGGCTTGATTACAAAACAATAACGGTAATAGACGCCATTGGCAATCAGTC
GCAGGACATTATAAACGCAATTAAAAAAAAAGGATCTAATGCTCTTGGAGCAGGTGATCAGGGAATAATATTTGGATATG
CTTGCGATGAAACAAAAAATTTTTTACCTGCTCCTTATGAACTTGCCAATTCAATTTTAAAAAAAGCTAGCAATCTTAGA
AAATCAGGAGCAATAGAATGGTTGAGACCCGACTCAAAATCTCAAGTTACTATAGAATACGATAAAAACAGAAGGCCTGT
AAAAATCAAAAATATTATAGTCTCTCACCAACATCATCCAAATATCTCCCAAAAACTAATAAGAGAACCAATAATTGAAG
AAATTATTAAGCCCACCATTCAAGACAAGTCAATAATTGATGAAAATACTACTTATTGTATTAATCCTTCTGGAAATTTT
GTAATTGGAGGACCTACCGGAGACACAGGTCTTACAGGAAGAAAAATTATTGCCGATAGCTATGGAGGATTTGCAAGGCA
TGGAGGAGGAGCATATAGCGGCAAAGATGCCACAAAAGTAGACAGATCAGCCGCCTACATGGCAAGATATATAGCAAAAA
ATATGGTGGCAGCCGGCATTTCTAAAGAATTCGAACTACAACTTGCATATGCAATTGGAATTGAAAATCCAATATCTATT
CAAATAACTTCAGGAATCAATGATCCCAAATATGCAAATAAAATATTAAATTTCATTGTCAATAACTTCGATTTAACTCC
CAACGGTATAATTGAAAAATTAAAACTAAAACAACCCATATATCTTAAAACTTGTACGTATGGTCAGTTGGGAAAGAATG
AATTTGAATGGGAAAAATTAGATTTTGTAAAAAAAATACAAACGGTGCTAAAAAAATGA

Upstream 100 bases:

>100_bases
ATTTACCAAACAATAAAGACAACCATATAGACTTTAATAAATTTTTAAAAACATCATCAATAAATTCAAGCAAAATGACA
AAGGAACTTATTAGGCTAAT

Downstream 100 bases:

>100_bases
AAAAAATAACAAGCTTTACAATAGATCATACAAAATTAAACCCCGGCATATATGTCTCAAGAAAAGACACCTTTGAAAAT
GTAATATTTACTACAATAGA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MNKILAANQTLTSEAVSEGHPDKIADQISDAILDEILKVDKNAKVACEVIITQNLVVIAGEINSPVKKTLDIKEIAKKII
KDIGYTNIDYGLDYKTITVIDAIGNQSQDIINAIKKKGSNALGAGDQGIIFGYACDETKNFLPAPYELANSILKKASNLR
KSGAIEWLRPDSKSQVTIEYDKNRRPVKIKNIIVSHQHHPNISQKLIREPIIEEIIKPTIQDKSIIDENTTYCINPSGNF
VIGGPTGDTGLTGRKIIADSYGGFARHGGGAYSGKDATKVDRSAAYMARYIAKNMVAAGISKEFELQLAYAIGIENPISI
QITSGINDPKYANKILNFIVNNFDLTPNGIIEKLKLKQPIYLKTCTYGQLGKNEFEWEKLDFVKKIQTVLKK

Sequences:

>Translated_392_residues
MNKILAANQTLTSEAVSEGHPDKIADQISDAILDEILKVDKNAKVACEVIITQNLVVIAGEINSPVKKTLDIKEIAKKII
KDIGYTNIDYGLDYKTITVIDAIGNQSQDIINAIKKKGSNALGAGDQGIIFGYACDETKNFLPAPYELANSILKKASNLR
KSGAIEWLRPDSKSQVTIEYDKNRRPVKIKNIIVSHQHHPNISQKLIREPIIEEIIKPTIQDKSIIDENTTYCINPSGNF
VIGGPTGDTGLTGRKIIADSYGGFARHGGGAYSGKDATKVDRSAAYMARYIAKNMVAAGISKEFELQLAYAIGIENPISI
QITSGINDPKYANKILNFIVNNFDLTPNGIIEKLKLKQPIYLKTCTYGQLGKNEFEWEKLDFVKKIQTVLKK
>Mature_392_residues
MNKILAANQTLTSEAVSEGHPDKIADQISDAILDEILKVDKNAKVACEVIITQNLVVIAGEINSPVKKTLDIKEIAKKII
KDIGYTNIDYGLDYKTITVIDAIGNQSQDIINAIKKKGSNALGAGDQGIIFGYACDETKNFLPAPYELANSILKKASNLR
KSGAIEWLRPDSKSQVTIEYDKNRRPVKIKNIIVSHQHHPNISQKLIREPIIEEIIKPTIQDKSIIDENTTYCINPSGNF
VIGGPTGDTGLTGRKIIADSYGGFARHGGGAYSGKDATKVDRSAAYMARYIAKNMVAAGISKEFELQLAYAIGIENPISI
QITSGINDPKYANKILNFIVNNFDLTPNGIIEKLKLKQPIYLKTCTYGQLGKNEFEWEKLDFVKKIQTVLKK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=372, Percent_Identity=47.3118279569892, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI5174529, Length=372, Percent_Identity=45.9677419354839, Blast_Score=335, Evalue=5e-92,
Organism=Escherichia coli, GI1789311, Length=376, Percent_Identity=49.2021276595745, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17551082, Length=392, Percent_Identity=45.9183673469388, Blast_Score=344, Evalue=4e-95,
Organism=Caenorhabditis elegans, GI17538494, Length=390, Percent_Identity=46.6666666666667, Blast_Score=338, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI17538498, Length=373, Percent_Identity=47.4530831099196, Blast_Score=337, Evalue=7e-93,
Organism=Caenorhabditis elegans, GI25145633, Length=377, Percent_Identity=46.9496021220159, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI32565905, Length=390, Percent_Identity=43.3333333333333, Blast_Score=294, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI32565911, Length=329, Percent_Identity=45.5927051671732, Blast_Score=281, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI25145635, Length=333, Percent_Identity=45.045045045045, Blast_Score=274, Evalue=7e-74,
Organism=Caenorhabditis elegans, GI32565903, Length=332, Percent_Identity=44.2771084337349, Blast_Score=263, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI32565909, Length=82, Percent_Identity=47.5609756097561, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323209, Length=379, Percent_Identity=47.4934036939314, Blast_Score=333, Evalue=3e-92,
Organism=Saccharomyces cerevisiae, GI6320710, Length=373, Percent_Identity=48.2573726541555, Blast_Score=333, Evalue=3e-92,
Organism=Drosophila melanogaster, GI24580527, Length=378, Percent_Identity=47.6190476190476, Blast_Score=322, Evalue=3e-88,
Organism=Drosophila melanogaster, GI24580525, Length=378, Percent_Identity=47.6190476190476, Blast_Score=322, Evalue=3e-88,
Organism=Drosophila melanogaster, GI45552159, Length=378, Percent_Identity=47.3544973544974, Blast_Score=321, Evalue=6e-88,
Organism=Drosophila melanogaster, GI24580529, Length=378, Percent_Identity=47.3544973544974, Blast_Score=321, Evalue=6e-88,
Organism=Drosophila melanogaster, GI24580537, Length=378, Percent_Identity=47.3544973544974, Blast_Score=321, Evalue=6e-88,
Organism=Drosophila melanogaster, GI24580533, Length=378, Percent_Identity=47.3544973544974, Blast_Score=321, Evalue=6e-88,
Organism=Drosophila melanogaster, GI24580535, Length=378, Percent_Identity=47.3544973544974, Blast_Score=321, Evalue=6e-88,
Organism=Drosophila melanogaster, GI24580531, Length=378, Percent_Identity=47.3544973544974, Blast_Score=321, Evalue=6e-88,
Organism=Drosophila melanogaster, GI24580541, Length=375, Percent_Identity=46.4, Blast_Score=305, Evalue=5e-83,
Organism=Drosophila melanogaster, GI24580539, Length=151, Percent_Identity=41.0596026490066, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43166; Mature: 43166

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS00376 ADOMET_SYNTHETASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKILAANQTLTSEAVSEGHPDKIADQISDAILDEILKVDKNAKVACEVIITQNLVVIAG
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEE
EINSPVKKTLDIKEIAKKIIKDIGYTNIDYGLDYKTITVIDAIGNQSQDIINAIKKKGSN
CCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCC
ALGAGDQGIIFGYACDETKNFLPAPYELANSILKKASNLRKSGAIEWLRPDSKSQVTIEY
CCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEEE
DKNRRPVKIKNIIVSHQHHPNISQKLIREPIIEEIIKPTIQDKSIIDENTTYCINPSGNF
CCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEECCCCCE
VIGGPTGDTGLTGRKIIADSYGGFARHGGGAYSGKDATKVDRSAAYMARYIAKNMVAAGI
EEECCCCCCCCCCCEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
SKEFELQLAYAIGIENPISIQITSGINDPKYANKILNFIVNNFDLTPNGIIEKLKLKQPI
CCCCEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCE
YLKTCTYGQLGKNEFEWEKLDFVKKIQTVLKK
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKILAANQTLTSEAVSEGHPDKIADQISDAILDEILKVDKNAKVACEVIITQNLVVIAG
CCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEE
EINSPVKKTLDIKEIAKKIIKDIGYTNIDYGLDYKTITVIDAIGNQSQDIINAIKKKGSN
CCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCC
ALGAGDQGIIFGYACDETKNFLPAPYELANSILKKASNLRKSGAIEWLRPDSKSQVTIEY
CCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEEE
DKNRRPVKIKNIIVSHQHHPNISQKLIREPIIEEIIKPTIQDKSIIDENTTYCINPSGNF
CCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEECCCCCE
VIGGPTGDTGLTGRKIIADSYGGFARHGGGAYSGKDATKVDRSAAYMARYIAKNMVAAGI
EEECCCCCCCCCCCEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
SKEFELQLAYAIGIENPISIQITSGINDPKYANKILNFIVNNFDLTPNGIIEKLKLKQPI
CCCCEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCE
YLKTCTYGQLGKNEFEWEKLDFVKKIQTVLKK
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA