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The map label for this gene is prfA [H]

Identifier: 51598456

GI number: 51598456

Start: 193392

End: 194465

Strand: Direct

Name: prfA [H]

Synonym: BG0194

Alternate gene names: 51598456

Gene position: 193392-194465 (Clockwise)

Preceding gene: 51598453

Following gene: 51598457

Centisome position: 21.39

GC content: 28.77

Gene sequence:

>1074_bases
ATGTTTTTAGAAAAATTAAATTCAGCAACAAGTAAGATTAAGTTAATAGAAGAAAAATTGCAAGATATAAATCTAATTAA
AAATCAAAAAAAATATTCAAAAATAATAAAAGAATATACATATTTAGAAAAAATAAATACTAAAAAAATTGAATACGAAA
AAATACTAAGTCAAATAAATGATAACAAAACCATTTTAGAAAAAGAAGATCAACAAGAAATGAAAGAGCTAATAAAACAA
GAACTAATTGATCTAGATAAAAAAAAGGAAGATCTTGAACATCAAATAAAAATACTGCTTTTACCTCAAGATGAAAATGA
TAGTAAAAATATAATAATTGAAATTAGAGCTGGAACAGGGGGAGAAGAAGCTGCTCTTTTTGCAAACAATCTTTACAGTA
TGTATATAAAATATTCGGAAAGAAAAAAATGGAAAACAGAAATCATAAACTTCAATGAAACAGAGCTTGGGGGGTTTAAA
GAAATAGTCTTTGAAATTAAAGGAAAAGATGTATTTAAAAAATTAAAATACGAAAGTGGAGTTCATAGAGTGCAAAGAAT
CCCCATAACAGAGTCTAACGGAAGGCTTCAAACCTCAGCTGCTACTGTAGCAGTACTACCTAATATTGAAGAAACTGAAA
TTGATATCAATGAAAAAGATTTAAGAATTGATGTTTACAGATCATCTGGAGCTGGTGGACAACACGTCAATACAACCGAT
TCTGCAGTAAGAATAACACACCTGCCAACAGGAATTGTTGTACAATGCCAAAACGAAAGAAGTCAACATAAAAACAAAGA
TCAAGCAATGAAAATACTAAGAGCAAGACTTTATGAATTTGAAGATTCTAAAAAACAAGAGCAAAGATCAAGCAATAGAA
AGCAACAAGTCGGCTCAGGAGATAGATCTGAAAGAATTAGAACATATAATTTCCCTCAAAATAGAATAACAGATCATAGA
GCAAACATCACTCTTTATAAATTAGAAGAATTTATGCAAGGAGAGCTTGATCCACTTCTTGATCCTCTAACAATAGAGCT
TCAAGAACAAAAATTTAAAAGCAACAACATATAG

Upstream 100 bases:

>100_bases
AAGCATAAAAATATGCATCCTATCAAGAAACTTGCTAAAAAGTCTAACAAAAAAATCTAAAATTAATATAAACTATTACT
ATCTTTCAGGAGAAATCATA

Downstream 100 bases:

>100_bases
TAATGAATGTAGCCGAAATTATAAATTATGCTAAAAGTAAAAATTTAGATACAATAGAAGCACTACTAATACTTGAATTG
ATTTTAAAAACCAGAAAAGA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MFLEKLNSATSKIKLIEEKLQDINLIKNQKKYSKIIKEYTYLEKINTKKIEYEKILSQINDNKTILEKEDQQEMKELIKQ
ELIDLDKKKEDLEHQIKILLLPQDENDSKNIIIEIRAGTGGEEAALFANNLYSMYIKYSERKKWKTEIINFNETELGGFK
EIVFEIKGKDVFKKLKYESGVHRVQRIPITESNGRLQTSAATVAVLPNIEETEIDINEKDLRIDVYRSSGAGGQHVNTTD
SAVRITHLPTGIVVQCQNERSQHKNKDQAMKILRARLYEFEDSKKQEQRSSNRKQQVGSGDRSERIRTYNFPQNRITDHR
ANITLYKLEEFMQGELDPLLDPLTIELQEQKFKSNNI

Sequences:

>Translated_357_residues
MFLEKLNSATSKIKLIEEKLQDINLIKNQKKYSKIIKEYTYLEKINTKKIEYEKILSQINDNKTILEKEDQQEMKELIKQ
ELIDLDKKKEDLEHQIKILLLPQDENDSKNIIIEIRAGTGGEEAALFANNLYSMYIKYSERKKWKTEIINFNETELGGFK
EIVFEIKGKDVFKKLKYESGVHRVQRIPITESNGRLQTSAATVAVLPNIEETEIDINEKDLRIDVYRSSGAGGQHVNTTD
SAVRITHLPTGIVVQCQNERSQHKNKDQAMKILRARLYEFEDSKKQEQRSSNRKQQVGSGDRSERIRTYNFPQNRITDHR
ANITLYKLEEFMQGELDPLLDPLTIELQEQKFKSNNI
>Mature_357_residues
MFLEKLNSATSKIKLIEEKLQDINLIKNQKKYSKIIKEYTYLEKINTKKIEYEKILSQINDNKTILEKEDQQEMKELIKQ
ELIDLDKKKEDLEHQIKILLLPQDENDSKNIIIEIRAGTGGEEAALFANNLYSMYIKYSERKKWKTEIINFNETELGGFK
EIVFEIKGKDVFKKLKYESGVHRVQRIPITESNGRLQTSAATVAVLPNIEETEIDINEKDLRIDVYRSSGAGGQHVNTTD
SAVRITHLPTGIVVQCQNERSQHKNKDQAMKILRARLYEFEDSKKQEQRSSNRKQQVGSGDRSERIRTYNFPQNRITDHR
ANITLYKLEEFMQGELDPLLDPLTIELQEQKFKSNNI

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=284, Percent_Identity=50.7042253521127, Blast_Score=295, Evalue=3e-80,
Organism=Homo sapiens, GI34577120, Length=336, Percent_Identity=40.1785714285714, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI166795305, Length=185, Percent_Identity=47.027027027027, Blast_Score=191, Evalue=7e-49,
Organism=Escherichia coli, GI1787462, Length=354, Percent_Identity=48.5875706214689, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI2367172, Length=287, Percent_Identity=41.4634146341463, Blast_Score=218, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI17542784, Length=320, Percent_Identity=35.9375, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=334, Percent_Identity=44.9101796407186, Blast_Score=245, Evalue=7e-66,
Organism=Drosophila melanogaster, GI19921226, Length=371, Percent_Identity=38.544474393531, Blast_Score=257, Evalue=9e-69,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41681; Mature: 41681

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLEKLNSATSKIKLIEEKLQDINLIKNQKKYSKIIKEYTYLEKINTKKIEYEKILSQIN
CCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
DNKTILEKEDQQEMKELIKQELIDLDKKKEDLEHQIKILLLPQDENDSKNIIIEIRAGTG
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEEEEEECCCC
GEEAALFANNLYSMYIKYSERKKWKTEIINFNETELGGFKEIVFEIKGKDVFKKLKYESG
CCHHHHHHHHHEEEEEECCCHHHHHHHHCCCCCCCCCCHHHHHEEECCHHHHHHHHHHHH
VHRVQRIPITESNGRLQTSAATVAVLPNIEETEIDINEKDLRIDVYRSSGAGGQHVNTTD
HHHHHCCCCCCCCCEEEECCEEEEEECCCCCCEECCCCCCEEEEEEECCCCCCCCCCCCC
SAVRITHLPTGIVVQCQNERSQHKNKDQAMKILRARLYEFEDSKKQEQRSSNRKQQVGSG
CEEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
DRSERIRTYNFPQNRITDHRANITLYKLEEFMQGELDPLLDPLTIELQEQKFKSNNI
CCCCCCEEECCCHHHCCCCCCCEEEEEHHHHHCCCCCCCCCCEEEEEHHHHHCCCCC
>Mature Secondary Structure
MFLEKLNSATSKIKLIEEKLQDINLIKNQKKYSKIIKEYTYLEKINTKKIEYEKILSQIN
CCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
DNKTILEKEDQQEMKELIKQELIDLDKKKEDLEHQIKILLLPQDENDSKNIIIEIRAGTG
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEEEEEECCCC
GEEAALFANNLYSMYIKYSERKKWKTEIINFNETELGGFKEIVFEIKGKDVFKKLKYESG
CCHHHHHHHHHEEEEEECCCHHHHHHHHCCCCCCCCCCHHHHHEEECCHHHHHHHHHHHH
VHRVQRIPITESNGRLQTSAATVAVLPNIEETEIDINEKDLRIDVYRSSGAGGQHVNTTD
HHHHHCCCCCCCCCEEEECCEEEEEECCCCCCEECCCCCCEEEEEEECCCCCCCCCCCCC
SAVRITHLPTGIVVQCQNERSQHKNKDQAMKILRARLYEFEDSKKQEQRSSNRKQQVGSG
CEEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
DRSERIRTYNFPQNRITDHRANITLYKLEEFMQGELDPLLDPLTIELQEQKFKSNNI
CCCCCCEEECCCHHHCCCCCCCEEEEEHHHHHCCCCCCCCCCEEEEEHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA