Click here to switch to the map view.

The map label for this gene is prfB [H]

Identifier: 51598335

GI number: 51598335

Start: 69997

End: 71049

Strand: Direct

Name: prfB [H]

Synonym: BG0073

Alternate gene names: 51598335

Gene position: 69997-71049 (Clockwise)

Preceding gene: 51598334

Following gene: 51598336

Centisome position: 7.74

GC content: 31.15

Gene sequence:

>1053_bases
TTGAACAAGCCGAAGGCATTTGGAGGAAGCTTTGACAAAAAAGAAATTCAAGATAAAATAGAAAAATACGAAAAAGAAAT
ACACCAGAAAAATTTTTGGAATGATCCTAAAAGAGCCCAAGAAGTTATAAAAGCTCAAAGTATCTTGAAAAATAAAATTG
ATCCGTGGGAAGAGCTAATAAACAAAATCAAAGATTTAAGCGATCTTTGTGAAATTGTTGAAAATGAAAAAGATATTAAC
AGCTTAGAAATCGAATTTAATGTACTTGAAAAACAGTACAAAAATTTACTCACAATTTCTTACTTTAAAGAAGAGCTTGA
CATAAACAGCGCTTTTTTAACTATTCATTCTGGTGCTGGAGGCACTGAAGCATGTGACTGGGTTGCAATGCTTTACAGAA
TGTACTCAAGATATGCTGAGAGAAAAAAATATAAAACAGAACTGATTGATTTGCTTGAAGCAGAAGGTGGAATTAAATCT
ATTACAATAGAAATCAAAGGTGAATATGCTTATGGACTTTTAAAAAGTGAAGTTGGAATACATCGCCTTATAAGAATTTC
TCCGTTTGATGCTGCTAAAAAAAGACATACCTCTTTTGCATCAGTATTTGTTGACCCTGTTATTGATGAGAAAATAGAAA
TAATAATTAAACCAGAAGATATAAGAATTGATACATATAGAGCATCGGGAGCTGGGGGGCAACACGTCAACAAAACATCC
TCTGCTGTAAGAATAACTCACATTGAAACAGGAATAGTAACTCAATCTCAAAGCGACCGAAGTCAGCACAAAAACAAAGA
TATGGCAATGAAAGTTTTAAAATCAAGACTTTACGAATACTATAAGAATAAAGAAGATAAAAAAAATAAATCCAAGCAAG
AAGTAAAAAAAGAAATTTCTTGGGGCAACCAAATAAGATCTTACGTATTTCAACCTTACAATTTAGTAAAAGATCACAGA
ACAAAATTTGAAAATTCAAACACCACTTCGGTTATGGACGGCAATATAGATAATTTCATAGAAGAGTATTTAAAATGGAA
AAGTTTAAACTAA

Upstream 100 bases:

>100_bases
TTTATAATGTTTATTTTATCTTTAATTCCAAATATAATTATATTAATATACAATAAATAATAGCGAGAAATAAAAATGAA
AGAAAAAATAAATATATTGT

Downstream 100 bases:

>100_bases
CCCTAGTAATGCCGTTGCTAATATTTACAATATATAAAATACATTCTCAAAGTAATATTGAATACAACTTTTCCTATATC
ATTAATACAAAAAAAGAAAA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MNKPKAFGGSFDKKEIQDKIEKYEKEIHQKNFWNDPKRAQEVIKAQSILKNKIDPWEELINKIKDLSDLCEIVENEKDIN
SLEIEFNVLEKQYKNLLTISYFKEELDINSAFLTIHSGAGGTEACDWVAMLYRMYSRYAERKKYKTELIDLLEAEGGIKS
ITIEIKGEYAYGLLKSEVGIHRLIRISPFDAAKKRHTSFASVFVDPVIDEKIEIIIKPEDIRIDTYRASGAGGQHVNKTS
SAVRITHIETGIVTQSQSDRSQHKNKDMAMKVLKSRLYEYYKNKEDKKNKSKQEVKKEISWGNQIRSYVFQPYNLVKDHR
TKFENSNTTSVMDGNIDNFIEEYLKWKSLN

Sequences:

>Translated_350_residues
MNKPKAFGGSFDKKEIQDKIEKYEKEIHQKNFWNDPKRAQEVIKAQSILKNKIDPWEELINKIKDLSDLCEIVENEKDIN
SLEIEFNVLEKQYKNLLTISYFKEELDINSAFLTIHSGAGGTEACDWVAMLYRMYSRYAERKKYKTELIDLLEAEGGIKS
ITIEIKGEYAYGLLKSEVGIHRLIRISPFDAAKKRHTSFASVFVDPVIDEKIEIIIKPEDIRIDTYRASGAGGQHVNKTS
SAVRITHIETGIVTQSQSDRSQHKNKDMAMKVLKSRLYEYYKNKEDKKNKSKQEVKKEISWGNQIRSYVFQPYNLVKDHR
TKFENSNTTSVMDGNIDNFIEEYLKWKSLN
>Mature_350_residues
MNKPKAFGGSFDKKEIQDKIEKYEKEIHQKNFWNDPKRAQEVIKAQSILKNKIDPWEELINKIKDLSDLCEIVENEKDIN
SLEIEFNVLEKQYKNLLTISYFKEELDINSAFLTIHSGAGGTEACDWVAMLYRMYSRYAERKKYKTELIDLLEAEGGIKS
ITIEIKGEYAYGLLKSEVGIHRLIRISPFDAAKKRHTSFASVFVDPVIDEKIEIIIKPEDIRIDTYRASGAGGQHVNKTS
SAVRITHIETGIVTQSQSDRSQHKNKDMAMKVLKSRLYEYYKNKEDKKNKSKQEVKKEISWGNQIRSYVFQPYNLVKDHR
TKFENSNTTSVMDGNIDNFIEEYLKWKSLN

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=289, Percent_Identity=37.0242214532872, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI34577120, Length=302, Percent_Identity=34.4370860927152, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI166795305, Length=184, Percent_Identity=40.2173913043478, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI2367172, Length=341, Percent_Identity=47.8005865102639, Blast_Score=327, Evalue=5e-91,
Organism=Escherichia coli, GI1787462, Length=245, Percent_Identity=40.4081632653061, Blast_Score=178, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17542784, Length=277, Percent_Identity=31.0469314079422, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=323, Percent_Identity=35.9133126934984, Blast_Score=142, Evalue=7e-35,
Organism=Drosophila melanogaster, GI19921226, Length=262, Percent_Identity=34.3511450381679, Blast_Score=144, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40742; Mature: 40742

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKPKAFGGSFDKKEIQDKIEKYEKEIHQKNFWNDPKRAQEVIKAQSILKNKIDPWEELI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
NKIKDLSDLCEIVENEKDINSLEIEFNVLEKQYKNLLTISYFKEELDINSAFLTIHSGAG
HHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
GTEACDWVAMLYRMYSRYAERKKYKTELIDLLEAEGGIKSITIEIKGEYAYGLLKSEVGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHH
HRLIRISPFDAAKKRHTSFASVFVDPVIDEKIEIIIKPEDIRIDTYRASGAGGQHVNKTS
HHHEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEECCCCCCCCCCCCC
SAVRITHIETGIVTQSQSDRSQHKNKDMAMKVLKSRLYEYYKNKEDKKNKSKQEVKKEIS
CEEEEEEEECCCEECCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
WGNQIRSYVFQPYNLVKDHRTKFENSNTTSVMDGNIDNFIEEYLKWKSLN
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKPKAFGGSFDKKEIQDKIEKYEKEIHQKNFWNDPKRAQEVIKAQSILKNKIDPWEELI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
NKIKDLSDLCEIVENEKDINSLEIEFNVLEKQYKNLLTISYFKEELDINSAFLTIHSGAG
HHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
GTEACDWVAMLYRMYSRYAERKKYKTELIDLLEAEGGIKSITIEIKGEYAYGLLKSEVGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHH
HRLIRISPFDAAKKRHTSFASVFVDPVIDEKIEIIIKPEDIRIDTYRASGAGGQHVNKTS
HHHEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEECCCCCCCCCCCCC
SAVRITHIETGIVTQSQSDRSQHKNKDMAMKVLKSRLYEYYKNKEDKKNKSKQEVKKEIS
CEEEEEEEECCCEECCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
WGNQIRSYVFQPYNLVKDHRTKFENSNTTSVMDGNIDNFIEEYLKWKSLN
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]