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The map label for this gene is priA [H]

Identifier: 51598277

GI number: 51598277

Start: 12587

End: 14569

Strand: Direct

Name: priA [H]

Synonym: BG0014

Alternate gene names: 51598277

Gene position: 12587-14569 (Clockwise)

Preceding gene: 51598276

Following gene: 51598280

Centisome position: 1.39

GC content: 25.62

Gene sequence:

>1983_bases
ATGGATGAACATTATCATTCAACTTATTACTATGAAATTGCAATAAATATTCCTTTAAATAAACTTTTTATTTACAGGTC
TAACTTGAATCTAGAAATTGGAATAAGAGTAATGGTTAATTTTAATGGCTCCAATAAAATTGGAATAATTACTAAAAAAT
ATCCTAAAAGCGAATTCAAAGAAAAATTTGAATTCAAAATAAAAGAAATTATTAAAATAATAGATAAAACTAAAATAATA
ACAGAACATAACATTGATTTAGCACATTGGATTAGTAGAAAAACATTTTCGGGATTTGGAGAAACTTTATTCTTTGGGTT
GCCCAAAAATTCAAAATCTGAAAAAAATCAAACATTGCCTTCAATAAATGAGTATCCGGATCATAAAAAGCACCTGCAAC
TAAACAACGAACAACAAAATATTTACAAAGAAATTATTGGGTCAGAAAAAAATAATGTTTTTTACCTTTTTGGAATACCT
GGATCTGGAAAAACTGAAATATTTATCAAATTGTGTGAATACTACTTAACACTAGAACAACAAGTACTCTTCTTGATTCC
TGAAATATCATTAGGATATCAAATAATAAAAAGAATAAAATATGCATTAAATATGCATCATAAAATTTATGAATATAACT
CTAAAGTTTCAAATTCTAATAAAAATTTAATATGGAATAAAGTCAAAAATGGAGAAAGTCTGATTGTTGTTGGCATTAAA
AGTGTACTAATGCTGCCTTTTACTAAATTAAAATTAATAATTATGGACGAAGAACACGAAACTACATACAAATCTGAAAA
TATTCCAAGATTTCACTCAAGACACATATCATTTTTTTTGCAAAAAAAATTTAATGCTAAATTCATAATGGGAAGTGCAA
CACCTTCTCTTGAAGCATACCACGCAATGAAACATAACCAAATAAAAAAAATAATAATGCAAAATAAATTCTTTCAAAGC
AAAATAGAAGATATAAAAATAATAAACATGAAAAAAGAACCTAGCACAATTTCATCAGAACTATTATACAGCATACAAAA
AAGTTTAAATAAAAAAAGACAATCTTTAATTTTCATTAATAAAAGAGGATATTTGAAAAATCTTGAATGCAACGAATGCG
GACATACAATTTATTGTCCAAATTGCTCATTTAGTCTGATTTACCACAAAAAAGAAAACAAACTTTTATGCCACTATTGT
AGTTATAAAACAAAAACAGCAAGTAATTGCCCTCAATGCGAATCAAAAGACATTAAATACAAAACTTATGGAATTCAACT
TGTTGAAAAAGAATTAAAAAAATTTTTGCCAAATGCAAAAATTGCAAGAATAGATTCCGATATTACAAAAATAGAAAATA
TAGATTCAATAATTAAGTTCGAAAACAAAGAAATAGACATACTTATCGGAACACAAATCATTGCAAAAGGATTCAATTTT
AAAAATATAAAAACACTAGGTATAATAAACGCAGATATTGGAATAGGATTGCCTGATTTTAGAAGTAGCGAAAGAATTTT
TACAACACTCTCGCAGATTATGGGAAGAGCCGCAAGATTTAAAGATGATAACACAATTATAATACAAACAAAAAATCCTA
ATTATTATGCCATAAAATGTGCTTATAAGAACCAATATGAAGAATTTTACGAGCAAGAGCTAGATATAAGAAAAAAATTA
AACTATCCTCCGTTTAACAAAATTATTAGAATAATATTTAGAAGCAAAAATGAAGAATCTGCTAAACAAAAATGTTGGGA
ATTCTTTGAAAAATCTAAAGAATTTTTGCAAGAAGGAATTGAGCACTTAGGCCCATCAGAAGCTATTATGAAAAAAATAT
CTAAAAATTATAGATACAATATAATATACTTGTCAAAATCCTATGGCTTACTTGAAAAACTAGTCAACAAGACAAGAGAA
AAGGTAAAACTAACAAATACTGTTTACATTGAAATAGATTATTATCCAATATCATTAATTTAG

Upstream 100 bases:

>100_bases
CCATTGGTTTTTACATACCTTCCTACAAATCAAATAAAAAATCCAGAATTAAAAAAAGAAATATGGCAGGATTTAAAAAT
AATCAAAGGCATAATAAAAT

Downstream 100 bases:

>100_bases
ACAAAATCTAGACCTTCTTTACTTAAAGACTTAAGAAACGTTGAAAGCACATAAAGAGCTTTATCATTGTGACCTCCTTC
GGGAATAATAAGATCAGCAT

Product: primosomal protein N

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 660; Mature: 660

Protein sequence:

>660_residues
MDEHYHSTYYYEIAINIPLNKLFIYRSNLNLEIGIRVMVNFNGSNKIGIITKKYPKSEFKEKFEFKIKEIIKIIDKTKII
TEHNIDLAHWISRKTFSGFGETLFFGLPKNSKSEKNQTLPSINEYPDHKKHLQLNNEQQNIYKEIIGSEKNNVFYLFGIP
GSGKTEIFIKLCEYYLTLEQQVLFLIPEISLGYQIIKRIKYALNMHHKIYEYNSKVSNSNKNLIWNKVKNGESLIVVGIK
SVLMLPFTKLKLIIMDEEHETTYKSENIPRFHSRHISFFLQKKFNAKFIMGSATPSLEAYHAMKHNQIKKIIMQNKFFQS
KIEDIKIINMKKEPSTISSELLYSIQKSLNKKRQSLIFINKRGYLKNLECNECGHTIYCPNCSFSLIYHKKENKLLCHYC
SYKTKTASNCPQCESKDIKYKTYGIQLVEKELKKFLPNAKIARIDSDITKIENIDSIIKFENKEIDILIGTQIIAKGFNF
KNIKTLGIINADIGIGLPDFRSSERIFTTLSQIMGRAARFKDDNTIIIQTKNPNYYAIKCAYKNQYEEFYEQELDIRKKL
NYPPFNKIIRIIFRSKNEESAKQKCWEFFEKSKEFLQEGIEHLGPSEAIMKKISKNYRYNIIYLSKSYGLLEKLVNKTRE
KVKLTNTVYIEIDYYPISLI

Sequences:

>Translated_660_residues
MDEHYHSTYYYEIAINIPLNKLFIYRSNLNLEIGIRVMVNFNGSNKIGIITKKYPKSEFKEKFEFKIKEIIKIIDKTKII
TEHNIDLAHWISRKTFSGFGETLFFGLPKNSKSEKNQTLPSINEYPDHKKHLQLNNEQQNIYKEIIGSEKNNVFYLFGIP
GSGKTEIFIKLCEYYLTLEQQVLFLIPEISLGYQIIKRIKYALNMHHKIYEYNSKVSNSNKNLIWNKVKNGESLIVVGIK
SVLMLPFTKLKLIIMDEEHETTYKSENIPRFHSRHISFFLQKKFNAKFIMGSATPSLEAYHAMKHNQIKKIIMQNKFFQS
KIEDIKIINMKKEPSTISSELLYSIQKSLNKKRQSLIFINKRGYLKNLECNECGHTIYCPNCSFSLIYHKKENKLLCHYC
SYKTKTASNCPQCESKDIKYKTYGIQLVEKELKKFLPNAKIARIDSDITKIENIDSIIKFENKEIDILIGTQIIAKGFNF
KNIKTLGIINADIGIGLPDFRSSERIFTTLSQIMGRAARFKDDNTIIIQTKNPNYYAIKCAYKNQYEEFYEQELDIRKKL
NYPPFNKIIRIIFRSKNEESAKQKCWEFFEKSKEFLQEGIEHLGPSEAIMKKISKNYRYNIIYLSKSYGLLEKLVNKTRE
KVKLTNTVYIEIDYYPISLI
>Mature_660_residues
MDEHYHSTYYYEIAINIPLNKLFIYRSNLNLEIGIRVMVNFNGSNKIGIITKKYPKSEFKEKFEFKIKEIIKIIDKTKII
TEHNIDLAHWISRKTFSGFGETLFFGLPKNSKSEKNQTLPSINEYPDHKKHLQLNNEQQNIYKEIIGSEKNNVFYLFGIP
GSGKTEIFIKLCEYYLTLEQQVLFLIPEISLGYQIIKRIKYALNMHHKIYEYNSKVSNSNKNLIWNKVKNGESLIVVGIK
SVLMLPFTKLKLIIMDEEHETTYKSENIPRFHSRHISFFLQKKFNAKFIMGSATPSLEAYHAMKHNQIKKIIMQNKFFQS
KIEDIKIINMKKEPSTISSELLYSIQKSLNKKRQSLIFINKRGYLKNLECNECGHTIYCPNCSFSLIYHKKENKLLCHYC
SYKTKTASNCPQCESKDIKYKTYGIQLVEKELKKFLPNAKIARIDSDITKIENIDSIIKFENKEIDILIGTQIIAKGFNF
KNIKTLGIINADIGIGLPDFRSSERIFTTLSQIMGRAARFKDDNTIIIQTKNPNYYAIKCAYKNQYEEFYEQELDIRKKL
NYPPFNKIIRIIFRSKNEESAKQKCWEFFEKSKEFLQEGIEHLGPSEAIMKKISKNYRYNIIYLSKSYGLLEKLVNKTRE
KVKLTNTVYIEIDYYPISLI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=528, Percent_Identity=28.219696969697, Blast_Score=273, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259
- InterPro:   IPR006935 [H]

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII [H]

EC number: NA

Molecular weight: Translated: 77599; Mature: 77599

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEHYHSTYYYEIAINIPLNKLFIYRSNLNLEIGIRVMVNFNGSNKIGIITKKYPKSEFK
CCCCEEEEEEEEEEEECCCEEEEEEEECCCEEEEEEEEEEECCCCEEEEEEECCCCHHHH
EKFEFKIKEIIKIIDKTKIITEHNIDLAHWISRKTFSGFGETLFFGLPKNSKSEKNQTLP
HHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHCCCC
SINEYPDHKKHLQLNNEQQNIYKEIIGSEKNNVFYLFGIPGSGKTEIFIKLCEYYLTLEQ
CCCCCCCCCHHEEECCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHC
QVLFLIPEISLGYQIIKRIKYALNMHHKIYEYNSKVSNSNKNLIWNKVKNGESLIVVGIK
EEEEEECCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEECCCCCEEEEEEHH
SVLMLPFTKLKLIIMDEEHETTYKSENIPRFHSRHISFFLQKKFNAKFIMGSATPSLEAY
HHHHCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHEEEECCCCEEEEECCCCCHHHH
HAMKHNQIKKIIMQNKFFQSKIEDIKIINMKKEPSTISSELLYSIQKSLNKKRQSLIFIN
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCEEEEEE
KRGYLKNLECNECGHTIYCPNCSFSLIYHKKENKLLCHYCSYKTKTASNCPQCESKDIKY
CCCCCCCCCCCCCCCEEECCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCEE
KTYGIQLVEKELKKFLPNAKIARIDSDITKIENIDSIIKFENKEIDILIGTQIIAKGFNF
EEHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHEECCCEEEEEECCHHHHCCCCC
KNIKTLGIINADIGIGLPDFRSSERIFTTLSQIMGRAARFKDDNTIIIQTKNPNYYAIKC
CCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEE
AYKNQYEEFYEQELDIRKKLNYPPFNKIIRIIFRSKNEESAKQKCWEFFEKSKEFLQEGI
EECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
EHLGPSEAIMKKISKNYRYNIIYLSKSYGLLEKLVNKTREKVKLTNTVYIEIDYYPISLI
HHCCCHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHEEEEEEEEEEEEEEEEEEC
>Mature Secondary Structure
MDEHYHSTYYYEIAINIPLNKLFIYRSNLNLEIGIRVMVNFNGSNKIGIITKKYPKSEFK
CCCCEEEEEEEEEEEECCCEEEEEEEECCCEEEEEEEEEEECCCCEEEEEEECCCCHHHH
EKFEFKIKEIIKIIDKTKIITEHNIDLAHWISRKTFSGFGETLFFGLPKNSKSEKNQTLP
HHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCHHCCCC
SINEYPDHKKHLQLNNEQQNIYKEIIGSEKNNVFYLFGIPGSGKTEIFIKLCEYYLTLEQ
CCCCCCCCCHHEEECCHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHC
QVLFLIPEISLGYQIIKRIKYALNMHHKIYEYNSKVSNSNKNLIWNKVKNGESLIVVGIK
EEEEEECCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEECCCCCEEEEEEHH
SVLMLPFTKLKLIIMDEEHETTYKSENIPRFHSRHISFFLQKKFNAKFIMGSATPSLEAY
HHHHCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHEEEECCCCEEEEECCCCCHHHH
HAMKHNQIKKIIMQNKFFQSKIEDIKIINMKKEPSTISSELLYSIQKSLNKKRQSLIFIN
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCEEEEEE
KRGYLKNLECNECGHTIYCPNCSFSLIYHKKENKLLCHYCSYKTKTASNCPQCESKDIKY
CCCCCCCCCCCCCCCEEECCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCEE
KTYGIQLVEKELKKFLPNAKIARIDSDITKIENIDSIIKFENKEIDILIGTQIIAKGFNF
EEHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHEECCCEEEEEECCHHHHCCCCC
KNIKTLGIINADIGIGLPDFRSSERIFTTLSQIMGRAARFKDDNTIIIQTKNPNYYAIKC
CCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEE
AYKNQYEEFYEQELDIRKKLNYPPFNKIIRIIFRSKNEESAKQKCWEFFEKSKEFLQEGI
EECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
EHLGPSEAIMKKISKNYRYNIIYLSKSYGLLEKLVNKTREKVKLTNTVYIEIDYYPISLI
HHCCCHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHEEEEEEEEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9228761; 9403685 [H]