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The map label for this gene is insA

Identifier: 51597557

GI number: 51597557

Start: 3834445

End: 3834708

Strand: Reverse

Name: insA

Synonym: YPTB3255

Alternate gene names: 51597557

Gene position: 3834708-3834445 (Counterclockwise)

Preceding gene: 51597558

Following gene: 51597556

Centisome position: 80.82

GC content: 48.11

Gene sequence:

>264_bases
ATGGCTTGCATTAATGTTCATTGTCCCCGTTGTGATTCTGCTTTGGTTTGTCGACATGGTCAGAACCCTAAGGGCCAAAG
CCGTTTCCGCTGCCGTGAGTGCCGCTCTGTATTCCAACTGACCTACACTTATCAGGCCAGAAAGCCCGGAGTTAAAGGGC
AGATCGTTGATATGGCCTTTAACGGCGCAGGTGTGTGGGATATGGCAAGAACGCTAAAAATCGGTATCAATACCGTTATC
CGCACTTTAAAAAACTCTTGCTGA

Upstream 100 bases:

>100_bases
GGTAAAGAATATAAATTAAAAATAGCCGAATAATATTAATTTCGGTAATGACTCCAATTGGTTGAATTTGATGTACAATG
CGATTTTTTGAGGTTTGCTC

Downstream 100 bases:

>100_bases
AGCGAATAACGTCCTCGCCAGTCGCTCATCTGTGAACTCGATGAGCAGTGGAGTTTTGTCGGCAACAAGGCCCTTCAGTA
GAAACTACGGAGTCATCAAT

Product: insertion element protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 87; Mature: 86

Protein sequence:

>87_residues
MACINVHCPRCDSALVCRHGQNPKGQSRFRCRECRSVFQLTYTYQARKPGVKGQIVDMAFNGAGVWDMARTLKIGINTVI
RTLKNSC

Sequences:

>Translated_87_residues
MACINVHCPRCDSALVCRHGQNPKGQSRFRCRECRSVFQLTYTYQARKPGVKGQIVDMAFNGAGVWDMARTLKIGINTVI
RTLKNSC
>Mature_86_residues
ACINVHCPRCDSALVCRHGQNPKGQSRFRCRECRSVFQLTYTYQARKPGVKGQIVDMAFNGAGVWDMARTLKIGINTVIR
TLKNSC

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG3677

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS1 elements insA family [H]

Homologues:

Organism=Escherichia coli, GI1789852, Length=87, Percent_Identity=42.5287356321839, Blast_Score=82, Evalue=6e-18,
Organism=Escherichia coli, GI1788204, Length=87, Percent_Identity=42.5287356321839, Blast_Score=82, Evalue=6e-18,
Organism=Escherichia coli, GI1786204, Length=87, Percent_Identity=42.5287356321839, Blast_Score=82, Evalue=6e-18,
Organism=Escherichia coli, GI1790747, Length=87, Percent_Identity=44.8275862068966, Blast_Score=82, Evalue=9e-18,
Organism=Escherichia coli, GI87081814, Length=87, Percent_Identity=42.5287356321839, Blast_Score=81, Evalue=1e-17,
Organism=Escherichia coli, GI1786471, Length=87, Percent_Identity=42.5287356321839, Blast_Score=81, Evalue=1e-17,
Organism=Escherichia coli, GI1786460, Length=87, Percent_Identity=42.5287356321839, Blast_Score=81, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003220 [H]

Pfam domain/function: PF03811 Ins_element1 [H]

EC number: NA

Molecular weight: Translated: 9778; Mature: 9646

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
11.5 %Cys+Met (Translated Protein)
8.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
10.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACINVHCPRCDSALVCRHGQNPKGQSRFRCRECRSVFQLTYTYQARKPGVKGQIVDMAF
CCEECCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEE
NGAGVWDMARTLKIGINTVIRTLKNSC
CCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ACINVHCPRCDSALVCRHGQNPKGQSRFRCRECRSVFQLTYTYQARKPGVKGQIVDMAF
CEECCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEE
NGAGVWDMARTLKIGINTVIRTLKNSC
CCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]