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The map label for this gene is amiD [H]

Identifier: 51596718

GI number: 51596718

Start: 2826648

End: 2827412

Strand: Reverse

Name: amiD [H]

Synonym: YPTB2395

Alternate gene names: 51596718

Gene position: 2827412-2826648 (Counterclockwise)

Preceding gene: 51596719

Following gene: 51596717

Centisome position: 59.59

GC content: 42.09

Gene sequence:

>765_bases
ATGTATAATATTGATTATAATAGTTTTCGTTCAGTAAAAGGATTCAATCGACGCGTTCGTTTTCTTGTGATGCATTATAC
GGCCCTCAATTTTAAAGATTCTATCGATGCACTCACGGGGCCTTCGGTAAGCGCTCATTACCTGGTCCCCGATCCGACGG
AACAAACTTATATTGATGCCGGTTTTAAGGATATGCGTATTTTTAATCTTGTTGATGAAAATGAGCGTGCCTGGCATGCA
GGAGTGAGTTATTGGGATGGTCGGAATAATCTTAATGACACCGCTATAGGGATTGAGACCGTTAATCTTGCTACTGATAA
CGATGGCGTATTCACTTTCCCGCCCTACAATGTTACCCAGATAGCGGCAATTAAAGCGTTGGCATCAAATATTCTTTATC
GTTTCCCTGATATTACGCCAGTCAATGTCGTTGGCCACAGTGATATTGCACCTGGTCGAAAAAGTGATCCAGGTGCAGCC
TTTCCGTGGAAGGCGCTTTATGATGCAGGTATTGGTGCCTGGTATGACGATGAAACTAAGCAACGGTATCTGGAGCAGTT
TCTTTGTGGCCTGCCATCAAAAAACGATATTATTTCAAAATTGAAACGATATGGTTATGACACGTCTGGCGCAGTCAGTG
AGGTGGGATATAATCAATTAATCCGTGCTTTCCAACTTCATTTTCGGCCATGTAATTATGATGGTATACCGGATGCAGAA
ACGGTTGCGATCCTCTATGCATTAGTCGATAAATATAAACCTTAA

Upstream 100 bases:

>100_bases
CAAAAAAAAATTAAATATTGATGCGGCGCATACATTGTGGCTGGCTTATATACAGTGATGTGTTAACACTTAAATTCATT
TTTATTGAGTAAGGATTCTT

Downstream 100 bases:

>100_bases
ATATATTTTTTCTACCGCTAATCATAGTTTAAAGGCTCAGCTCATTCTTTGGGCCTTTCTCTACGTTATTCCCCGCACTA
TTTAATGAATTCTGCATGCC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MYNIDYNSFRSVKGFNRRVRFLVMHYTALNFKDSIDALTGPSVSAHYLVPDPTEQTYIDAGFKDMRIFNLVDENERAWHA
GVSYWDGRNNLNDTAIGIETVNLATDNDGVFTFPPYNVTQIAAIKALASNILYRFPDITPVNVVGHSDIAPGRKSDPGAA
FPWKALYDAGIGAWYDDETKQRYLEQFLCGLPSKNDIISKLKRYGYDTSGAVSEVGYNQLIRAFQLHFRPCNYDGIPDAE
TVAILYALVDKYKP

Sequences:

>Translated_254_residues
MYNIDYNSFRSVKGFNRRVRFLVMHYTALNFKDSIDALTGPSVSAHYLVPDPTEQTYIDAGFKDMRIFNLVDENERAWHA
GVSYWDGRNNLNDTAIGIETVNLATDNDGVFTFPPYNVTQIAAIKALASNILYRFPDITPVNVVGHSDIAPGRKSDPGAA
FPWKALYDAGIGAWYDDETKQRYLEQFLCGLPSKNDIISKLKRYGYDTSGAVSEVGYNQLIRAFQLHFRPCNYDGIPDAE
TVAILYALVDKYKP
>Mature_254_residues
MYNIDYNSFRSVKGFNRRVRFLVMHYTALNFKDSIDALTGPSVSAHYLVPDPTEQTYIDAGFKDMRIFNLVDENERAWHA
GVSYWDGRNNLNDTAIGIETVNLATDNDGVFTFPPYNVTQIAAIKALASNILYRFPDITPVNVVGHSDIAPGRKSDPGAA
FPWKALYDAGIGAWYDDETKQRYLEQFLCGLPSKNDIISKLKRYGYDTSGAVSEVGYNQLIRAFQLHFRPCNYDGIPDAE
TVAILYALVDKYKP

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=253, Percent_Identity=43.0830039525692, Blast_Score=183, Evalue=9e-48,
Organism=Escherichia coli, GI1786300, Length=121, Percent_Identity=38.8429752066116, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 28634; Mature: 28634

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNIDYNSFRSVKGFNRRVRFLVMHYTALNFKDSIDALTGPSVSAHYLVPDPTEQTYIDA
CCCCCCHHHHHHHHHHHHEEEEEEEHHHCCCCHHHHHHCCCCCEEEEECCCCCCCCEECC
GFKDMRIFNLVDENERAWHAGVSYWDGRNNLNDTAIGIETVNLATDNDGVFTFPPYNVTQ
CCCCEEEEEEECCCCCCEECCCEEECCCCCCCCCEEEEEEEEEEECCCCEEEECCCCHHH
IAAIKALASNILYRFPDITPVNVVGHSDIAPGRKSDPGAAFPWKALYDAGIGAWYDDETK
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHH
QRYLEQFLCGLPSKNDIISKLKRYGYDTSGAVSEVGYNQLIRAFQLHFRPCNYDGIPDAE
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
TVAILYALVDKYKP
HHHHHHHHHHHCCC
>Mature Secondary Structure
MYNIDYNSFRSVKGFNRRVRFLVMHYTALNFKDSIDALTGPSVSAHYLVPDPTEQTYIDA
CCCCCCHHHHHHHHHHHHEEEEEEEHHHCCCCHHHHHHCCCCCEEEEECCCCCCCCEECC
GFKDMRIFNLVDENERAWHAGVSYWDGRNNLNDTAIGIETVNLATDNDGVFTFPPYNVTQ
CCCCEEEEEEECCCCCCEECCCEEECCCCCCCCCEEEEEEEEEEECCCCEEEECCCCHHH
IAAIKALASNILYRFPDITPVNVVGHSDIAPGRKSDPGAAFPWKALYDAGIGAWYDDETK
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHH
QRYLEQFLCGLPSKNDIISKLKRYGYDTSGAVSEVGYNQLIRAFQLHFRPCNYDGIPDAE
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
TVAILYALVDKYKP
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]