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The map label for this gene is eda

Identifier: 51596376

GI number: 51596376

Start: 2416657

End: 2417298

Strand: Direct

Name: eda

Synonym: YPTB2050

Alternate gene names: 51596376

Gene position: 2416657-2417298 (Clockwise)

Preceding gene: 161760575

Following gene: 51596377

Centisome position: 50.93

GC content: 52.18

Gene sequence:

>642_bases
ATGAAAAGCTGGAAAACGAGCGCAGAACAGATTATGACCGCAGGTCCTGTTGTCCCGGTGATCGTGGTCAATAAGCTCGA
GCAAGCAGTACCGTTGGCAAAAGCTCTGGTGGCAGGCGGTGTTCGCGTACTGGAAGTGACGTTGCGTACCCCTTGTGCTG
TGGAGGCCATCCGCGCGATTGCCAAAGAAGTTCCTGAGGCAATTATTGGTGCTGGAACCGTGCTTAACCCACAACAACTG
GCTGAAGTGGTTGAAGCTGGCGCTCAGTTTGCCATTAGCCCTGGTTTGACTGATGAACTGCTTAAAGCGGCCTGCGAAGG
GTCTATCCCATTGATTCCAGGTATCAGCACCGTTTCAGAACTGATGCTGGGGATGAGCTATGGTTTGCGTGAGTTTAAAT
TCTTCCCGGCTGAAGCTAACGGTGGTGTCAAAGCGTTGCAAGCCATTGGCGGCCCATTCTCGCAAGTCCGTTTCTGCCCA
ACTGGCGGTATCACACCGAATAACTACCGTGATTATTTAGCACTGAAGAGTGTATTGTGCATTGGTGGCTCATGGTTGGT
TCCTGCTGACGCGTTGGAGAGTGGTGACTACGCGCGCATTACTGAATTAGCGAAGCAAGCGGTTGAAGGTGCGGCAGCAT
AA

Upstream 100 bases:

>100_bases
TCGCAGTTGTTCCATCGGGAGCGGCGATCAGAGTGCCATATTTCAACTAACACCTTCGGGTGAGTACAATGCCAGTCGGC
ATAAGTGGAGATAACAACTA

Downstream 100 bases:

>100_bases
GCCGCTCTAACTCCCTGACATAACGGGAGAATCCGTATATTTTCACCAAAGACAGAGCCTCTGCATTGCAGGGGTTTTGT
TTTTTCAGAATCGCTAACTT

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKSWKTSAEQIMTAGPVVPVIVVNKLEQAVPLAKALVAGGVRVLEVTLRTPCAVEAIRAIAKEVPEAIIGAGTVLNPQQL
AEVVEAGAQFAISPGLTDELLKAACEGSIPLIPGISTVSELMLGMSYGLREFKFFPAEANGGVKALQAIGGPFSQVRFCP
TGGITPNNYRDYLALKSVLCIGGSWLVPADALESGDYARITELAKQAVEGAAA

Sequences:

>Translated_213_residues
MKSWKTSAEQIMTAGPVVPVIVVNKLEQAVPLAKALVAGGVRVLEVTLRTPCAVEAIRAIAKEVPEAIIGAGTVLNPQQL
AEVVEAGAQFAISPGLTDELLKAACEGSIPLIPGISTVSELMLGMSYGLREFKFFPAEANGGVKALQAIGGPFSQVRFCP
TGGITPNNYRDYLALKSVLCIGGSWLVPADALESGDYARITELAKQAVEGAAA
>Mature_213_residues
MKSWKTSAEQIMTAGPVVPVIVVNKLEQAVPLAKALVAGGVRVLEVTLRTPCAVEAIRAIAKEVPEAIIGAGTVLNPQQL
AEVVEAGAQFAISPGLTDELLKAACEGSIPLIPGISTVSELMLGMSYGLREFKFFPAEANGGVKALQAIGGPFSQVRFCP
TGGITPNNYRDYLALKSVLCIGGSWLVPADALESGDYARITELAKQAVEGAAA

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=211, Percent_Identity=88.1516587677725, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI48994954, Length=167, Percent_Identity=28.1437125748503, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 22133; Mature: 22133

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSWKTSAEQIMTAGPVVPVIVVNKLEQAVPLAKALVAGGVRVLEVTLRTPCAVEAIRAI
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
AKEVPEAIIGAGTVLNPQQLAEVVEAGAQFAISPGLTDELLKAACEGSIPLIPGISTVSE
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCCCCCHHHHHH
LMLGMSYGLREFKFFPAEANGGVKALQAIGGPFSQVRFCPTGGITPNNYRDYLALKSVLC
HHHHHHHCHHHHEECCCCCCCHHHHHHHHCCCHHHEEECCCCCCCCCCHHHHHHHHHHHH
IGGSWLVPADALESGDYARITELAKQAVEGAAA
HCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKSWKTSAEQIMTAGPVVPVIVVNKLEQAVPLAKALVAGGVRVLEVTLRTPCAVEAIRAI
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
AKEVPEAIIGAGTVLNPQQLAEVVEAGAQFAISPGLTDELLKAACEGSIPLIPGISTVSE
HHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCCCCCCHHHHHH
LMLGMSYGLREFKFFPAEANGGVKALQAIGGPFSQVRFCPTGGITPNNYRDYLALKSVLC
HHHHHHHCHHHHEECCCCCCCHHHHHHHHCCCHHHEEECCCCCCCCCCHHHHHHHHHHHH
IGGSWLVPADALESGDYARITELAKQAVEGAAA
HCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8145647 [H]