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The map label for this gene is ddl [H]

Identifier: 50843773

GI number: 50843773

Start: 2546113

End: 2547096

Strand: Direct

Name: ddl [H]

Synonym: PPA2338

Alternate gene names: 50843773

Gene position: 2546113-2547096 (Clockwise)

Preceding gene: 50843772

Following gene: 50843774

Centisome position: 99.45

GC content: 58.43

Gene sequence:

>984_bases
ATGCCATCATTGTCTTGGCACTGGTTGAATGGGGGCATGTTGCAACCAGTCGTCGTCCTTGCCGGAGGACTCAGCCACCA
GCGCGATATCTCTCTGAAGTCGGGTCGCACCGTTGCACAAGCGCTGCGGCGGGTCGGACACGAAGTCGTGGAAGCCGATA
TTGACTCCGCACTCATTGAGACCTTGCGTGCCAACGACGGTGCCGTCGTGTTTCCCATGTTGCACGGTGGTCTTGGCGAA
AATGGTGCCCTTCTTGAAGTACTTCAGCTCATGGGCATCCCCTATGTGGGCTCCAGTCCAGCAACCTGCCGTATCTCTTT
CGATAAGTCCATCGCATCTCGCGCCGTGGCCGCAGCCGGGATCGTCACACCACAGCAGGTCGCGTTACCCCATGACGTCT
TCCGTGAGCTTGGCGCTCAGACGGTCATGCGTTCGATCGCCGAAAAGTTTGGCCTTCCGGTCATCGTTAAGCCGGCACGT
GGGGGCTCAAGCCTCGGCGTCACAAAAGTCGATGGCGTCGACGATCTTCCTCAGGCCGTCGCGAACGCCTATGCCTATGA
CGACATGGTTGTAGTCGAGGAATTCATTGTGGGCACTGAGCTCGCAATAGGCATGATCACGACGTCTGAAGGCACGCGTG
TGCTGCCAGCCGTCGAGATTCGCCCTGTCGGTGGTGTTTATGATTATTCAGCGATGTACACCGGTGGTGAGACACGACTA
ACAGCTCCTGCAGACATTAGCGATACAGCGGCCCAAACCGCGACGGCGATGGCCCGAGTCGTGCAAAAGGAGCTCGATTT
CTCCGGGATATCTCGTGTCGATGCGATCGTGGACGAGTCCGGTCGCCCAGTTTTCTTGGAGGCCGGTGCTGCTCCCGGGA
TGACAGCTACTTCGCTCGTACCCGTGGCTATGAAAGCTGCCGGTCTAGACCTTGGCGAGGTGTGCTCTCGACTCGTCGAT
GACGTCGCTCGCAACCATGGCTGA

Upstream 100 bases:

>100_bases
AGCATCGGCCCGGCGTGCTTCAACCACGCCTCAGGCGGACGCTTTCCCAAGCTGCTCCCACGTTGCTGCCATTGTCTTAT
AGATACGGGGAGGGTCGGTT

Downstream 100 bases:

>100_bases
CAGTGTGCACATGAAGGGCTCCCGCCACGCCGTGCGCGTCAAGCAGGCATCTGTCGTCTTGCTCGGCGTCGTCCTTGCCA
GTGTGATGGTCTTCCTCGGA

Product: D-alanine--D-alanine ligase B

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MPSLSWHWLNGGMLQPVVVLAGGLSHQRDISLKSGRTVAQALRRVGHEVVEADIDSALIETLRANDGAVVFPMLHGGLGE
NGALLEVLQLMGIPYVGSSPATCRISFDKSIASRAVAAAGIVTPQQVALPHDVFRELGAQTVMRSIAEKFGLPVIVKPAR
GGSSLGVTKVDGVDDLPQAVANAYAYDDMVVVEEFIVGTELAIGMITTSEGTRVLPAVEIRPVGGVYDYSAMYTGGETRL
TAPADISDTAAQTATAMARVVQKELDFSGISRVDAIVDESGRPVFLEAGAAPGMTATSLVPVAMKAAGLDLGEVCSRLVD
DVARNHG

Sequences:

>Translated_327_residues
MPSLSWHWLNGGMLQPVVVLAGGLSHQRDISLKSGRTVAQALRRVGHEVVEADIDSALIETLRANDGAVVFPMLHGGLGE
NGALLEVLQLMGIPYVGSSPATCRISFDKSIASRAVAAAGIVTPQQVALPHDVFRELGAQTVMRSIAEKFGLPVIVKPAR
GGSSLGVTKVDGVDDLPQAVANAYAYDDMVVVEEFIVGTELAIGMITTSEGTRVLPAVEIRPVGGVYDYSAMYTGGETRL
TAPADISDTAAQTATAMARVVQKELDFSGISRVDAIVDESGRPVFLEAGAAPGMTATSLVPVAMKAAGLDLGEVCSRLVD
DVARNHG
>Mature_326_residues
PSLSWHWLNGGMLQPVVVLAGGLSHQRDISLKSGRTVAQALRRVGHEVVEADIDSALIETLRANDGAVVFPMLHGGLGEN
GALLEVLQLMGIPYVGSSPATCRISFDKSIASRAVAAAGIVTPQQVALPHDVFRELGAQTVMRSIAEKFGLPVIVKPARG
GSSLGVTKVDGVDDLPQAVANAYAYDDMVVVEEFIVGTELAIGMITTSEGTRVLPAVEIRPVGGVYDYSAMYTGGETRLT
APADISDTAAQTATAMARVVQKELDFSGISRVDAIVDESGRPVFLEAGAAPGMTATSLVPVAMKAAGLDLGEVCSRLVDD
VARNHG

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=310, Percent_Identity=34.8387096774194, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1786579, Length=259, Percent_Identity=28.957528957529, Blast_Score=108, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 34131; Mature: 33999

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLSWHWLNGGMLQPVVVLAGGLSHQRDISLKSGRTVAQALRRVGHEVVEADIDSALIE
CCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TLRANDGAVVFPMLHGGLGENGALLEVLQLMGIPYVGSSPATCRISFDKSIASRAVAAAG
HHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHC
IVTPQQVALPHDVFRELGAQTVMRSIAEKFGLPVIVKPARGGSSLGVTKVDGVDDLPQAV
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEECCCCHHHHHHH
ANAYAYDDMVVVEEFIVGTELAIGMITTSEGTRVLPAVEIRPVGGVYDYSAMYTGGETRL
HHHHHHCCHHHHHHHHHHHHHEEEEEECCCCCEECCEEEEEECCCEEECCEEEECCCEEE
TAPADISDTAAQTATAMARVVQKELDFSGISRVDAIVDESGRPVFLEAGAAPGMTATSLV
ECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHH
PVAMKAAGLDLGEVCSRLVDDVARNHG
HHHHHHHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSLSWHWLNGGMLQPVVVLAGGLSHQRDISLKSGRTVAQALRRVGHEVVEADIDSALIE
CCCCEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TLRANDGAVVFPMLHGGLGENGALLEVLQLMGIPYVGSSPATCRISFDKSIASRAVAAAG
HHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHC
IVTPQQVALPHDVFRELGAQTVMRSIAEKFGLPVIVKPARGGSSLGVTKVDGVDDLPQAV
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEECCCCHHHHHHH
ANAYAYDDMVVVEEFIVGTELAIGMITTSEGTRVLPAVEIRPVGGVYDYSAMYTGGETRL
HHHHHHCCHHHHHHHHHHHHHEEEEEECCCCCEECCEEEEEECCCEEECCEEEECCCEEE
TAPADISDTAAQTATAMARVVQKELDFSGISRVDAIVDESGRPVFLEAGAAPGMTATSLV
ECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHH
PVAMKAAGLDLGEVCSRLVDDVARNHG
HHHHHHHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA