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The map label for this gene is uidA [H]

Identifier: 50843768

GI number: 50843768

Start: 2537391

End: 2539172

Strand: Direct

Name: uidA [H]

Synonym: PPA2333

Alternate gene names: 50843768

Gene position: 2537391-2539172 (Clockwise)

Preceding gene: 50843767

Following gene: 50843769

Centisome position: 99.11

GC content: 60.38

Gene sequence:

>1782_bases
ATGCTGCGACCACAGGACACCGCCACCCGGGACACGAAGTGTCTGTCGGGGATGTGGGACTTTGCCTTCGACGCCGAGGA
CCGCGGCCAGACAGAGAAATGGTTCACTCGACCGTTGCCCACGTGCACCGAGATGGCAGTTCCGGCGTCGTACCAGGATC
TCTCCACTGATCCCGCCGCCCGCGATCACGTTGGCCCAGTCTGGTACCAACGTGAGGTTCGCATTCCGCGCGGTTGGACC
GATGATCGCGTCGTCGTCCATTTCGAGTCTGCGACTCATGCCGCCACCGTCTGGGTCAACGACGTCGAGGTCGTCAGACA
TGTTGGCGGGTACCTCCCTTTCGAAGCCGATATCACCGATCATGTCTGCGCGGGCAAAAAATGTCGGCTCACGGTTCGTG
TCGATAATCGGTTGTCCTTCCAGACCATTCCGCCGGGGATTATCGTCGACTCTCCCGAGGGCCCGAAGCAGAAGTACTGG
CACGACTTCTTCAATTACGCCGGCATCCACCGCGACGTGTGGCTGTGTCGCCGCCCCCAGGCCCACATTGAGGACGTCAC
CATCACCACTGACATTGACGGAAATGACGGAATCGTCACCTGGTCGGTTCTTTTGCACGATGCTGAACCTTTAACGACTC
GGGTGACAATTTTCGACACCGAGGGCAACGTGGTCGCTGGGGGAACCAGTGCGAACGCCCATGCCCGCATCCCGTCGGTC
CATCTCTGGCAGCCAGGCCACGGGTATCTTTACGAGGCCGAAATCTCCCTAGTCGACGGTGATCAGGTAATCGATACTTA
CCAGCAGGTCTTCGGCGTCCGAACTATCCGTGTCGACGGCGCCCGCCTCCTCGTCAACGGCGAGCCCGTGCACTTGACCG
GATTCGGCATGCACGAGGATCACCAGACCATCGGCAAGGCCCACAACGATGCCCTCATGCTGCGCGACGCCGCCTGTCTA
GAGTGGATCGGGGCGAATTCGCTGCGCACGTCCCATTACCCCTACTCCGAGCGTATCCTCGACTACGCCGACCGTCATGG
TCTGCTCGTTATCGACGAAACCCCTGCTGTCGGCATAAACATGGGTCTGGGTGGCGGCATTTTTGGCGCCCAGGGCTACC
CGACTTTCTCCGCGGAGACCATCAATGACAAGACCCAAAAGGTTCATGCCCAGGTCATCCGAGACCTTATCGCTCGAGAT
AAAAACCACCCCAGCGTCATCATCTGGTCCATCGCCAATGAGCCAGAGTCTGAGACTGAAGCCGCCGAAAACTACTTCCT
CCCCCTCTTCGACGTGGCACGCGCAGCCGACCCCACCCGTCCGGTGAGTTTCGTCAACGTCATGCTCGCTCCGTTCGGAA
AGTGTCGCGTATCGCAGTACTCCGACGTTTTGCTGCTCAACCGCTATTATGGCTGGTACGTCGACACTGGCGACCTGGCC
GCCGCTGAGCGTCACTGGCGAGAAGAGATGGCAGGTTGGGCCAGCGAGAACAAGCCGATCATCATCACCGAATACGGCGC
CGACACCATGCCCGGTCTGCACCAAATCCCCGCCCAGCCATGGAGCGAGGAGTACCAGGTCGAAGTGCTCAAGATGAACG
AGCGGGTCTTCGACTCTTTCGACGCCATCGTCGGTGAGCACATCTGGAACTTCGCGGACTTCGCGACCACCAGTGGCACG
ATGCGCGTCGGAGGCAACCGAAAGGGCATCTTCACCCGGGATCGCCAGCCCAAGTCGGCCGCCTTCCACCTGCGCAAGAG
GTGGCGTGGCGTCGCGCAGTAA

Upstream 100 bases:

>100_bases
TGGCTTTCGCTCTTCGCCCAGGAGATGACCGCGCCATCAAGGTCGTAGCCGACGCCGCCCAGGACTAGAAACCAAACGCC
ACAACCGGAAGGAGATCATG

Downstream 100 bases:

>100_bases
CGTCCGCACAAGTAGACCCGGTGGGCGTGAGAGCGAGCGCCCTTACGCCCACCCCCGCCCGGACCGCTTGCCCGTCTGGC
CACTCCTCCATGGGAACCAC

Product: beta-D-glucuronidase

Products: NA

Alternate protein names: GUS; Beta-D-glucuronoside glucuronosohydrolase [H]

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MLRPQDTATRDTKCLSGMWDFAFDAEDRGQTEKWFTRPLPTCTEMAVPASYQDLSTDPAARDHVGPVWYQREVRIPRGWT
DDRVVVHFESATHAATVWVNDVEVVRHVGGYLPFEADITDHVCAGKKCRLTVRVDNRLSFQTIPPGIIVDSPEGPKQKYW
HDFFNYAGIHRDVWLCRRPQAHIEDVTITTDIDGNDGIVTWSVLLHDAEPLTTRVTIFDTEGNVVAGGTSANAHARIPSV
HLWQPGHGYLYEAEISLVDGDQVIDTYQQVFGVRTIRVDGARLLVNGEPVHLTGFGMHEDHQTIGKAHNDALMLRDAACL
EWIGANSLRTSHYPYSERILDYADRHGLLVIDETPAVGINMGLGGGIFGAQGYPTFSAETINDKTQKVHAQVIRDLIARD
KNHPSVIIWSIANEPESETEAAENYFLPLFDVARAADPTRPVSFVNVMLAPFGKCRVSQYSDVLLLNRYYGWYVDTGDLA
AAERHWREEMAGWASENKPIIITEYGADTMPGLHQIPAQPWSEEYQVEVLKMNERVFDSFDAIVGEHIWNFADFATTSGT
MRVGGNRKGIFTRDRQPKSAAFHLRKRWRGVAQ

Sequences:

>Translated_593_residues
MLRPQDTATRDTKCLSGMWDFAFDAEDRGQTEKWFTRPLPTCTEMAVPASYQDLSTDPAARDHVGPVWYQREVRIPRGWT
DDRVVVHFESATHAATVWVNDVEVVRHVGGYLPFEADITDHVCAGKKCRLTVRVDNRLSFQTIPPGIIVDSPEGPKQKYW
HDFFNYAGIHRDVWLCRRPQAHIEDVTITTDIDGNDGIVTWSVLLHDAEPLTTRVTIFDTEGNVVAGGTSANAHARIPSV
HLWQPGHGYLYEAEISLVDGDQVIDTYQQVFGVRTIRVDGARLLVNGEPVHLTGFGMHEDHQTIGKAHNDALMLRDAACL
EWIGANSLRTSHYPYSERILDYADRHGLLVIDETPAVGINMGLGGGIFGAQGYPTFSAETINDKTQKVHAQVIRDLIARD
KNHPSVIIWSIANEPESETEAAENYFLPLFDVARAADPTRPVSFVNVMLAPFGKCRVSQYSDVLLLNRYYGWYVDTGDLA
AAERHWREEMAGWASENKPIIITEYGADTMPGLHQIPAQPWSEEYQVEVLKMNERVFDSFDAIVGEHIWNFADFATTSGT
MRVGGNRKGIFTRDRQPKSAAFHLRKRWRGVAQ
>Mature_593_residues
MLRPQDTATRDTKCLSGMWDFAFDAEDRGQTEKWFTRPLPTCTEMAVPASYQDLSTDPAARDHVGPVWYQREVRIPRGWT
DDRVVVHFESATHAATVWVNDVEVVRHVGGYLPFEADITDHVCAGKKCRLTVRVDNRLSFQTIPPGIIVDSPEGPKQKYW
HDFFNYAGIHRDVWLCRRPQAHIEDVTITTDIDGNDGIVTWSVLLHDAEPLTTRVTIFDTEGNVVAGGTSANAHARIPSV
HLWQPGHGYLYEAEISLVDGDQVIDTYQQVFGVRTIRVDGARLLVNGEPVHLTGFGMHEDHQTIGKAHNDALMLRDAACL
EWIGANSLRTSHYPYSERILDYADRHGLLVIDETPAVGINMGLGGGIFGAQGYPTFSAETINDKTQKVHAQVIRDLIARD
KNHPSVIIWSIANEPESETEAAENYFLPLFDVARAADPTRPVSFVNVMLAPFGKCRVSQYSDVLLLNRYYGWYVDTGDLA
AAERHWREEMAGWASENKPIIITEYGADTMPGLHQIPAQPWSEEYQVEVLKMNERVFDSFDAIVGEHIWNFADFATTSGT
MRVGGNRKGIFTRDRQPKSAAFHLRKRWRGVAQ

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=623, Percent_Identity=40.2889245585875, Blast_Score=448, Evalue=1e-126,
Organism=Escherichia coli, GI1787903, Length=597, Percent_Identity=49.5812395309883, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI48994920, Length=502, Percent_Identity=27.0916334661355, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI1786539, Length=475, Percent_Identity=23.7894736842105, Blast_Score=96, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17510825, Length=619, Percent_Identity=37.4798061389338, Blast_Score=406, Evalue=1e-113,
Organism=Drosophila melanogaster, GI62471735, Length=626, Percent_Identity=39.6166134185303, Blast_Score=438, Evalue=1e-123,
Organism=Drosophila melanogaster, GI19922582, Length=626, Percent_Identity=39.6166134185303, Blast_Score=438, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24655438, Length=626, Percent_Identity=39.6166134185303, Blast_Score=438, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24651745, Length=636, Percent_Identity=38.3647798742138, Blast_Score=413, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.31 [H]

Molecular weight: Translated: 66712; Mature: 66712

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00719 GLYCOSYL_HYDROL_F2_1 ; PS00608 GLYCOSYL_HYDROL_F2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRPQDTATRDTKCLSGMWDFAFDAEDRGQTEKWFTRPLPTCTEMAVPASYQDLSTDPAA
CCCCCCCCCCHHHHHHCCCCEEECCCCCCCCHHHHCCCCCCHHHHCCCCCCCCCCCCCCC
RDHVGPVWYQREVRIPRGWTDDRVVVHFESATHAATVWVNDVEVVRHVGGYLPFEADITD
CCCCCCEEEEEEEECCCCCCCCEEEEEEECCCCEEEEEECHHHHHHHHCCCCCEECCCCH
HVCAGKKCRLTVRVDNRLSFQTIPPGIIVDSPEGPKQKYWHDFFNYAGIHRDVWLCRRPQ
HHCCCCEEEEEEEECCCEEEEECCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCC
AHIEDVTITTDIDGNDGIVTWSVLLHDAEPLTTRVTIFDTEGNVVAGGTSANAHARIPSV
CCCCEEEEEEECCCCCCEEEEEEEEECCCCCEEEEEEEECCCCEEECCCCCCCCEECCEE
HLWQPGHGYLYEAEISLVDGDQVIDTYQQVFGVRTIRVDGARLLVNGEPVHLTGFGMHED
EEECCCCCEEEEEEEEEECCHHHHHHHHHHHCEEEEEECCEEEEECCCEEEEEECCCCCC
HQTIGKAHNDALMLRDAACLEWIGANSLRTSHYPYSERILDYADRHGLLVIDETPAVGIN
HHHHHCCCCCEEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCEEEE
MGLGGGIFGAQGYPTFSAETINDKTQKVHAQVIRDLIARDKNHPSVIIWSIANEPESETE
ECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHH
AAENYFLPLFDVARAADPTRPVSFVNVMLAPFGKCRVSQYSDVLLLNRYYGWYVDTGDLA
HHHCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCEEEECCCHH
AAERHWREEMAGWASENKPIIITEYGADTMPGLHQIPAQPWSEEYQVEVLKMNERVFDSF
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHCCCCCCCCCCEEEEEECCHHHHHHH
DAIVGEHIWNFADFATTSGTMRVGGNRKGIFTRDRQPKSAAFHLRKRWRGVAQ
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLRPQDTATRDTKCLSGMWDFAFDAEDRGQTEKWFTRPLPTCTEMAVPASYQDLSTDPAA
CCCCCCCCCCHHHHHHCCCCEEECCCCCCCCHHHHCCCCCCHHHHCCCCCCCCCCCCCCC
RDHVGPVWYQREVRIPRGWTDDRVVVHFESATHAATVWVNDVEVVRHVGGYLPFEADITD
CCCCCCEEEEEEEECCCCCCCCEEEEEEECCCCEEEEEECHHHHHHHHCCCCCEECCCCH
HVCAGKKCRLTVRVDNRLSFQTIPPGIIVDSPEGPKQKYWHDFFNYAGIHRDVWLCRRPQ
HHCCCCEEEEEEEECCCEEEEECCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCC
AHIEDVTITTDIDGNDGIVTWSVLLHDAEPLTTRVTIFDTEGNVVAGGTSANAHARIPSV
CCCCEEEEEEECCCCCCEEEEEEEEECCCCCEEEEEEEECCCCEEECCCCCCCCEECCEE
HLWQPGHGYLYEAEISLVDGDQVIDTYQQVFGVRTIRVDGARLLVNGEPVHLTGFGMHED
EEECCCCCEEEEEEEEEECCHHHHHHHHHHHCEEEEEECCEEEEECCCEEEEEECCCCCC
HQTIGKAHNDALMLRDAACLEWIGANSLRTSHYPYSERILDYADRHGLLVIDETPAVGIN
HHHHHCCCCCEEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCEEEE
MGLGGGIFGAQGYPTFSAETINDKTQKVHAQVIRDLIARDKNHPSVIIWSIANEPESETE
ECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHH
AAENYFLPLFDVARAADPTRPVSFVNVMLAPFGKCRVSQYSDVLLLNRYYGWYVDTGDLA
HHHCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCEEEECCCHH
AAERHWREEMAGWASENKPIIITEYGADTMPGLHQIPAQPWSEEYQVEVLKMNERVFDSF
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHCCCCCCCCCCEEEEEECCHHHHHHH
DAIVGEHIWNFADFATTSGTMRVGGNRKGIFTRDRQPKSAAFHLRKRWRGVAQ
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3534890; 8125312; 2103475; 9097039; 9278503 [H]