Click here to switch to the map view.

The map label for this gene is est [H]

Identifier: 50843749

GI number: 50843749

Start: 2512492

End: 2513421

Strand: Direct

Name: est [H]

Synonym: PPA2312

Alternate gene names: 50843749

Gene position: 2512492-2513421 (Clockwise)

Preceding gene: 50843748

Following gene: 50843766

Centisome position: 98.13

GC content: 61.72

Gene sequence:

>930_bases
ATGAAACTGGATCCACGACTGGCAGAAATCCTGGGTCGTCAACCGAAGGAAGAACAAACGCTCGCCGAGAAGCGGGCGCG
TCAGAATGACGTGGCCGCCCGCCTGCGGGGAAAAATAACGCCTGCGGTCCCTGATTCGGTGATTCTCGAGGACCGCACGG
TCCCCGGACGGACTCACCCGATCCCGGTACTCATTCAGCGGCCGAAGGGGCTCCCAAACCCCACTCCAGTACTGGTATGG
GCCCACGGCGGGGCTTTTGTTCACGGTTCCCCACGCACCGTGCTCTCCCGCACAGCCAGCATCGCCCAGCGAGCGGGGGT
ATGTGTCGTCAGTGTCGATTACCGATTGCTGCCCGAGCACAACTACCCCGAGAACCTACATGATGTCGTTGACGTCGTGC
GGTGGGTAGCCAAAACCGGACTTGACAAGGGGTTTGACACTTCACGCATCCTCGTAGGGGGCGATTCCGCGGGTGGAAAC
CTCGCCGCTGCGACGGGGATCATGTGCCGCGACGAGGGCAACCCCGCAATTCTTGGGCAAATCCTGGAGGTGCCGATTCT
CGACCTTTCTGACACCTCCGAAGCAATGGCCGAGGCTCGTCGTGACGCCCCTGACCTAGCGGCCGCGCTGCACCACAGCC
ACGTGCGTTATCTGTCTCACGGAGCAAGTCTCGACAACCCGCTGACTAACCCTGCCTGCCTGGAGAACGCGCACGGCCTT
CCGCCGACATTTTTGCTCAACTGTGAGGTGGATCCGCTGCGTGACGACTCGATCGCGTGGCAGCACAAGCTCCAGGACGC
CGGGGTGCCGGTAGAACTGACAACAGTCCGTGGCATGGCTCATGGCACCCAAAGTTTCACCCTCGCCTTCCCCCAGGCCC
GCGACGCCGAAGACAAAATCATCACCTGGCTGCGAGATCTCATCTCCTGA

Upstream 100 bases:

>100_bases
CTCGTAAAGACGGTGGGCTATCCCGGACTGTTCACTGCAGCGATCATCCTAGCCATCCTGGGGTCGTTGTGTATCACACG
AGTGAGGAGGGTGAAGTGAC

Downstream 100 bases:

>100_bases
TGCCGTAAAACGGCCGGGCCCGGGATTTTTCGTCACCGGGTCCGGCCGCCTCGGTTCCTGTGGAAGACCTTTCGTGAACA
CGCTCCGCGTAAAAACGCGG

Product: lipase/esterase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKLDPRLAEILGRQPKEEQTLAEKRARQNDVAARLRGKITPAVPDSVILEDRTVPGRTHPIPVLIQRPKGLPNPTPVLVW
AHGGAFVHGSPRTVLSRTASIAQRAGVCVVSVDYRLLPEHNYPENLHDVVDVVRWVAKTGLDKGFDTSRILVGGDSAGGN
LAAATGIMCRDEGNPAILGQILEVPILDLSDTSEAMAEARRDAPDLAAALHHSHVRYLSHGASLDNPLTNPACLENAHGL
PPTFLLNCEVDPLRDDSIAWQHKLQDAGVPVELTTVRGMAHGTQSFTLAFPQARDAEDKIITWLRDLIS

Sequences:

>Translated_309_residues
MKLDPRLAEILGRQPKEEQTLAEKRARQNDVAARLRGKITPAVPDSVILEDRTVPGRTHPIPVLIQRPKGLPNPTPVLVW
AHGGAFVHGSPRTVLSRTASIAQRAGVCVVSVDYRLLPEHNYPENLHDVVDVVRWVAKTGLDKGFDTSRILVGGDSAGGN
LAAATGIMCRDEGNPAILGQILEVPILDLSDTSEAMAEARRDAPDLAAALHHSHVRYLSHGASLDNPLTNPACLENAHGL
PPTFLLNCEVDPLRDDSIAWQHKLQDAGVPVELTTVRGMAHGTQSFTLAFPQARDAEDKIITWLRDLIS
>Mature_309_residues
MKLDPRLAEILGRQPKEEQTLAEKRARQNDVAARLRGKITPAVPDSVILEDRTVPGRTHPIPVLIQRPKGLPNPTPVLVW
AHGGAFVHGSPRTVLSRTASIAQRAGVCVVSVDYRLLPEHNYPENLHDVVDVVRWVAKTGLDKGFDTSRILVGGDSAGGN
LAAATGIMCRDEGNPAILGQILEVPILDLSDTSEAMAEARRDAPDLAAALHHSHVRYLSHGASLDNPLTNPACLENAHGL
PPTFLLNCEVDPLRDDSIAWQHKLQDAGVPVELTTVRGMAHGTQSFTLAFPQARDAEDKIITWLRDLIS

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786682, Length=235, Percent_Identity=26.8085106382979, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17567059, Length=319, Percent_Identity=25.0783699059561, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI72001146, Length=127, Percent_Identity=37.7952755905512, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 33518; Mature: 33518

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDPRLAEILGRQPKEEQTLAEKRARQNDVAARLRGKITPAVPDSVILEDRTVPGRTHP
CCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCC
IPVLIQRPKGLPNPTPVLVWAHGGAFVHGSPRTVLSRTASIAQRAGVCVVSVDYRLLPEH
CEEEEECCCCCCCCCCEEEEECCCEEEECCCHHHHHHHHHHHHHCCEEEEEECEEECCCC
NYPENLHDVVDVVRWVAKTGLDKGFDTSRILVGGDSAGGNLAAATGIMCRDEGNPAILGQ
CCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEHHCCEEEECCCCHHHHHH
ILEVPILDLSDTSEAMAEARRDAPDLAAALHHSHVRYLSHGASLDNPLTNPACLENAHGL
HHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCC
PPTFLLNCEVDPLRDDSIAWQHKLQDAGVPVELTTVRGMAHGTQSFTLAFPQARDAEDKI
CCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEHHHHCCCCEEEEECCCCCCCHHHH
ITWLRDLIS
HHHHHHHHC
>Mature Secondary Structure
MKLDPRLAEILGRQPKEEQTLAEKRARQNDVAARLRGKITPAVPDSVILEDRTVPGRTHP
CCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCC
IPVLIQRPKGLPNPTPVLVWAHGGAFVHGSPRTVLSRTASIAQRAGVCVVSVDYRLLPEH
CEEEEECCCCCCCCCCEEEEECCCEEEECCCHHHHHHHHHHHHHCCEEEEEECEEECCCC
NYPENLHDVVDVVRWVAKTGLDKGFDTSRILVGGDSAGGNLAAATGIMCRDEGNPAILGQ
CCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEHHCCEEEECCCCHHHHHH
ILEVPILDLSDTSEAMAEARRDAPDLAAALHHSHVRYLSHGASLDNPLTNPACLENAHGL
HHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCC
PPTFLLNCEVDPLRDDSIAWQHKLQDAGVPVELTTVRGMAHGTQSFTLAFPQARDAEDKI
CCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEHHHHCCCCEEEEECCCCCCCHHHH
ITWLRDLIS
HHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]