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The map label for this gene is lacF [H]

Identifier: 50843731

GI number: 50843731

Start: 2489527

End: 2490435

Strand: Reverse

Name: lacF [H]

Synonym: PPA2294

Alternate gene names: 50843731

Gene position: 2490435-2489527 (Counterclockwise)

Preceding gene: 50843732

Following gene: 50843730

Centisome position: 97.27

GC content: 55.78

Gene sequence:

>909_bases
ATGTCCTCATCCACGATGGCTAGTCGCAAGGCCAGACACGAGGCGCTAACTGGCTGGTTATTCGCACTGCCATTCACCAT
CGTCTTCATCATCGTGTTCGTTGCTCCCATCATTGAGAGCATCCGGGCTGGGTTCCACAAGCAGGTTGCCGAGGGAGGCG
GCCTTTATGGCGGAGGCCGGACAGTGGAGAAATTTGTCGGTCTAGAGAACTTCCAGGACGTATGCACGAATCCAGCCTTC
TGGCACGGCATCGGTCGCGTCATGCTATTTGGTGTCTTCCAAATCCCGATCATGATCCTTGCTGCACTGTTCCTCGCTCT
AGTTATCGACTCGTACTTGGTGCGCCATCTCGGGTTCTGGCGCCTTTCGTACTTCTTGCCCTACGCGATCCCAGGGCTTG
TGGCCGCGATTATGTGGGCCTACCTCTACGTGCCCGAGATTTCCCCTTTCGCGGGACATTTGAACTTCAGTCTCTTAAGT
TCTCACGTCATCCTCGCATCAATGGCCAATATGACGACGTGGACCTTTACCGGCTACAACATGTTGATCTTCTTAGCTGC
CCTACAGGCGATTCCCTCGGATCTCTACGAGGCCGCACGTATCGACGGCGCCTCCGGTTGGGAGATCATGACGAGAATTA
AGATCCCAATGGTTCGAGGCGCGGCTATGTTGGCAGTCCTGCTGTCCATCATCGGTACTGTGCAATTGTTCAACGAGCCG
ACCGTTTTGGCCACCGTTGCGACTTGGATGCCGCGTGATTACACGCCCATGATGATGGCTTACAACACTATGAACGGAAA
TATTAGCCCGAGCGGGGCCGGTCCGGCCTCGGCGGTGTCCATCATGATGGCTTTGGTCGCCGGGGTGCTCGCAATGATCT
ACGCCCTGGTCCAGAGGAAGGCGGACTGA

Upstream 100 bases:

>100_bases
GACAGTTTTGATCTGTCATTGACGCAGTCCGCGGCGATCGCCATGGTCGCCGAACGGTGCGGGAAGATCCGCACCGTGAC
AATTCTTCACAGGAGTCGGC

Downstream 100 bases:

>100_bases
CATGGCACACGGCAAGACCGCCGTCACCAAGGATGGCATCCCTTACCGCCCCTCATTGGATGGTCTCCCGCCTCGATCGA
TTGCCCCCTCCAAGGCGGCC

Product: binding-protein-dependent transport system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MSSSTMASRKARHEALTGWLFALPFTIVFIIVFVAPIIESIRAGFHKQVAEGGGLYGGGRTVEKFVGLENFQDVCTNPAF
WHGIGRVMLFGVFQIPIMILAALFLALVIDSYLVRHLGFWRLSYFLPYAIPGLVAAIMWAYLYVPEISPFAGHLNFSLLS
SHVILASMANMTTWTFTGYNMLIFLAALQAIPSDLYEAARIDGASGWEIMTRIKIPMVRGAAMLAVLLSIIGTVQLFNEP
TVLATVATWMPRDYTPMMMAYNTMNGNISPSGAGPASAVSIMMALVAGVLAMIYALVQRKAD

Sequences:

>Translated_302_residues
MSSSTMASRKARHEALTGWLFALPFTIVFIIVFVAPIIESIRAGFHKQVAEGGGLYGGGRTVEKFVGLENFQDVCTNPAF
WHGIGRVMLFGVFQIPIMILAALFLALVIDSYLVRHLGFWRLSYFLPYAIPGLVAAIMWAYLYVPEISPFAGHLNFSLLS
SHVILASMANMTTWTFTGYNMLIFLAALQAIPSDLYEAARIDGASGWEIMTRIKIPMVRGAAMLAVLLSIIGTVQLFNEP
TVLATVATWMPRDYTPMMMAYNTMNGNISPSGAGPASAVSIMMALVAGVLAMIYALVQRKAD
>Mature_301_residues
SSSTMASRKARHEALTGWLFALPFTIVFIIVFVAPIIESIRAGFHKQVAEGGGLYGGGRTVEKFVGLENFQDVCTNPAFW
HGIGRVMLFGVFQIPIMILAALFLALVIDSYLVRHLGFWRLSYFLPYAIPGLVAAIMWAYLYVPEISPFAGHLNFSLLSS
HVILASMANMTTWTFTGYNMLIFLAALQAIPSDLYEAARIDGASGWEIMTRIKIPMVRGAAMLAVLLSIIGTVQLFNEPT
VLATVATWMPRDYTPMMMAYNTMNGNISPSGAGPASAVSIMMALVAGVLAMIYALVQRKAD

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789861, Length=305, Percent_Identity=24.5901639344262, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32991; Mature: 32859

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSTMASRKARHEALTGWLFALPFTIVFIIVFVAPIIESIRAGFHKQVAEGGGLYGGGR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
TVEKFVGLENFQDVCTNPAFWHGIGRVMLFGVFQIPIMILAALFLALVIDSYLVRHLGFW
HHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLSYFLPYAIPGLVAAIMWAYLYVPEISPFAGHLNFSLLSSHVILASMANMTTWTFTGYN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECHHH
MLIFLAALQAIPSDLYEAARIDGASGWEIMTRIKIPMVRGAAMLAVLLSIIGTVQLFNEP
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TVLATVATWMPRDYTPMMMAYNTMNGNISPSGAGPASAVSIMMALVAGVLAMIYALVQRK
HHHHHHHHHCCCCCCHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
AD
CC
>Mature Secondary Structure 
SSSTMASRKARHEALTGWLFALPFTIVFIIVFVAPIIESIRAGFHKQVAEGGGLYGGGR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
TVEKFVGLENFQDVCTNPAFWHGIGRVMLFGVFQIPIMILAALFLALVIDSYLVRHLGFW
HHHHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLSYFLPYAIPGLVAAIMWAYLYVPEISPFAGHLNFSLLSSHVILASMANMTTWTFTGYN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECHHH
MLIFLAALQAIPSDLYEAARIDGASGWEIMTRIKIPMVRGAAMLAVLLSIIGTVQLFNEP
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TVLATVATWMPRDYTPMMMAYNTMNGNISPSGAGPASAVSIMMALVAGVLAMIYALVQRK
HHHHHHHHHCCCCCCHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
AD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]