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The map label for this gene is ynfJ [C]

Identifier: 50843687

GI number: 50843687

Start: 2437409

End: 2439136

Strand: Direct

Name: ynfJ [C]

Synonym: PPA2253

Alternate gene names: 50843687

Gene position: 2437409-2439136 (Clockwise)

Preceding gene: 50843686

Following gene: 50843690

Centisome position: 95.2

GC content: 62.27

Gene sequence:

>1728_bases
ATGACCGAGACTGCCATCCACCCCGAGGCCCGGGCCGCATGGGCCAAACAACTCAGGCGCCTGCGCCGTGCCCTCGTCCC
GCACCGTCCCAAGGCCTTCCGAGACTCCCTAAACCGTTGGCTGCTTATGGGCACAGTCATCGGTATCGTCTCTGGCGTCG
GTGCCATTATCTTCTTCTTCTGTCTGAAAACCGGCACGCATTGGCTACTCGAGACGTTGGGCGGCTATACCCCACCCGGC
ACCTTGGGGGAGGGCGGCGGTGTCGCCGGCACCAGGCTCACCCGCCCCTGGGCCATCCCGCTCATCGTCGCCGGAGGCGC
CCTGGTTTCGGGAACCTTGGTGTACTTCGTCGCGCCTACCGCCAAAGGCCATGGCACTGACAACGCCATTCATGCCGTCC
ATCACGACCCGACAGCTCTCAAGGGAAAAGTCGCAGCGGTCAAGATCATCGCATCCTCCCTCGTAATAGGTTCCGGCGGT
TCGGGTGGACGTGAGGGCCCCACTGCCCAGATCAGCGCGACCGTCATCTCCTCACTGTGTAAGAAGCTCAAAATCGCCAC
CCCAGACGCCCGGATTGCCGTCTCAGCAGCCACTGCCTCAGGCATCGGTGCCATCTTCCGCGCCCCGCTGGGCGGTGCGT
TACTAGGTGCGGAGCTGCTCTACCGCGACGACATGGAATCCGACGCGATAATGCCTTCCCTGGTGTCGTCGATCGTCGCC
TTCGTGGTCTTCGGGTCGGTATACGGATTCGAGCCAATCTTCGGATCGCTCAGTGGAGTGCAATTCTCCCAGCCTTTGCA
ACTCATCTGGTTCGTCCTACTGGGCCTCACTGCTGGCCTCATGGGCAAGCTTTATGTTGAGGTCTTCTACAGCGGCACCA
CGCTTTTTGACCGTCTCGACAAGGTTCCTGGCTGGCTCGTCCCGGCTATCGGTGGTCTGGGAGTCGGGCTGCTTGGACTC
CTCATACCGGCAGCACTGGGCACCGGTTACGGCTCAGTGCAACAAGAGATGTTCGCCAACAACCTCGTGCGGATGCCGCT
GCTCATGGTGCTGGCAATCCCCTTCGCCAAGATCCTCTCGACGACCCTGTCCATCGGATCGGGCGGTTCCGGCGGCGTCT
TCGGCCCTGGCATGGTCATCGGCGGAGCCACTGGCGCGGCACTGTGGCGCCTCCTCGAGGGGCTGCCAGGTATCCCATCC
TCACCGATGAGTTTCGTCATTGTCGGCATGATCGCCTGCTTCGGTGCGGTTGCCCATGCCCCACTCGGCGTGCTGCTCAT
GGTTGGCGAGATGACCGGAAACCTGTCGATGCTCGCTCCTGGCATGATCGCCGTCGCCGTCGCTGGCCGAGTTGTCGGGG
ACACTTCGATCTACACCTCTCAGCTCAAGGATCGCCTGGAGGGCCGACGCGTCCATGCCATGGGGCGCGTCACGAAACTA
TCAGAGCCTAAATCTGACGACGAGGTAGCTAGCCCGAAGCCCGCTATCAAGAAGCCATCCGGAAAGAATGCTTCCCTCTC
GAAGCCTGCTGCTGAGCCGCTTGCCAAACCGCAAGCCAAGCCCGCAACAGTTCCCTCCCCCCATCCGACCACGGCTCCCA
CCCCAGCCACTCACCAACGTGATACGCCTCGGATCGTCCCGGAAATTATTCGGGCCAAAGACCGTAAACACGAGCTGCTC
GCTCGGTCCCCCAAACCCCCGAACGACCCGGGACCGTCATCCGCTTGA

Upstream 100 bases:

>100_bases
TCGACACTGTCGTCACCGGATTCACCTTATTTACCCGCGCAGCCGAGGCCAGCGGACACGGCACCCCCAACCGCCCTTGC
GCAGAGGATTCCAACCAGCA

Downstream 100 bases:

>100_bases
GTTCAGTCGGTCGTGGGATGGAAGGGGCTGCGTCCGGCGTGATGGGACCATCCCACTCCAATGACATAGAGGATGACGAC
GAGCGCCCCCGTTGACATCA

Product: putative chloride channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 575; Mature: 574

Protein sequence:

>575_residues
MTETAIHPEARAAWAKQLRRLRRALVPHRPKAFRDSLNRWLLMGTVIGIVSGVGAIIFFFCLKTGTHWLLETLGGYTPPG
TLGEGGGVAGTRLTRPWAIPLIVAGGALVSGTLVYFVAPTAKGHGTDNAIHAVHHDPTALKGKVAAVKIIASSLVIGSGG
SGGREGPTAQISATVISSLCKKLKIATPDARIAVSAATASGIGAIFRAPLGGALLGAELLYRDDMESDAIMPSLVSSIVA
FVVFGSVYGFEPIFGSLSGVQFSQPLQLIWFVLLGLTAGLMGKLYVEVFYSGTTLFDRLDKVPGWLVPAIGGLGVGLLGL
LIPAALGTGYGSVQQEMFANNLVRMPLLMVLAIPFAKILSTTLSIGSGGSGGVFGPGMVIGGATGAALWRLLEGLPGIPS
SPMSFVIVGMIACFGAVAHAPLGVLLMVGEMTGNLSMLAPGMIAVAVAGRVVGDTSIYTSQLKDRLEGRRVHAMGRVTKL
SEPKSDDEVASPKPAIKKPSGKNASLSKPAAEPLAKPQAKPATVPSPHPTTAPTPATHQRDTPRIVPEIIRAKDRKHELL
ARSPKPPNDPGPSSA

Sequences:

>Translated_575_residues
MTETAIHPEARAAWAKQLRRLRRALVPHRPKAFRDSLNRWLLMGTVIGIVSGVGAIIFFFCLKTGTHWLLETLGGYTPPG
TLGEGGGVAGTRLTRPWAIPLIVAGGALVSGTLVYFVAPTAKGHGTDNAIHAVHHDPTALKGKVAAVKIIASSLVIGSGG
SGGREGPTAQISATVISSLCKKLKIATPDARIAVSAATASGIGAIFRAPLGGALLGAELLYRDDMESDAIMPSLVSSIVA
FVVFGSVYGFEPIFGSLSGVQFSQPLQLIWFVLLGLTAGLMGKLYVEVFYSGTTLFDRLDKVPGWLVPAIGGLGVGLLGL
LIPAALGTGYGSVQQEMFANNLVRMPLLMVLAIPFAKILSTTLSIGSGGSGGVFGPGMVIGGATGAALWRLLEGLPGIPS
SPMSFVIVGMIACFGAVAHAPLGVLLMVGEMTGNLSMLAPGMIAVAVAGRVVGDTSIYTSQLKDRLEGRRVHAMGRVTKL
SEPKSDDEVASPKPAIKKPSGKNASLSKPAAEPLAKPQAKPATVPSPHPTTAPTPATHQRDTPRIVPEIIRAKDRKHELL
ARSPKPPNDPGPSSA
>Mature_574_residues
TETAIHPEARAAWAKQLRRLRRALVPHRPKAFRDSLNRWLLMGTVIGIVSGVGAIIFFFCLKTGTHWLLETLGGYTPPGT
LGEGGGVAGTRLTRPWAIPLIVAGGALVSGTLVYFVAPTAKGHGTDNAIHAVHHDPTALKGKVAAVKIIASSLVIGSGGS
GGREGPTAQISATVISSLCKKLKIATPDARIAVSAATASGIGAIFRAPLGGALLGAELLYRDDMESDAIMPSLVSSIVAF
VVFGSVYGFEPIFGSLSGVQFSQPLQLIWFVLLGLTAGLMGKLYVEVFYSGTTLFDRLDKVPGWLVPAIGGLGVGLLGLL
IPAALGTGYGSVQQEMFANNLVRMPLLMVLAIPFAKILSTTLSIGSGGSGGVFGPGMVIGGATGAALWRLLEGLPGIPSS
PMSFVIVGMIACFGAVAHAPLGVLLMVGEMTGNLSMLAPGMIAVAVAGRVVGDTSIYTSQLKDRLEGRRVHAMGRVTKLS
EPKSDDEVASPKPAIKKPSGKNASLSKPAAEPLAKPQAKPATVPSPHPTTAPTPATHQRDTPRIVPEIIRAKDRKHELLA
RSPKPPNDPGPSSA

Specific function: Unknown

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081943, Length=346, Percent_Identity=32.9479768786127, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1786350, Length=398, Percent_Identity=26.6331658291457, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 59555; Mature: 59423

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETAIHPEARAAWAKQLRRLRRALVPHRPKAFRDSLNRWLLMGTVIGIVSGVGAIIFFF
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CLKTGTHWLLETLGGYTPPGTLGEGGGVAGTRLTRPWAIPLIVAGGALVSGTLVYFVAPT
HHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHCEEEEEEECC
AKGHGTDNAIHAVHHDPTALKGKVAAVKIIASSLVIGSGGSGGREGPTAQISATVISSLC
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHH
KKLKIATPDARIAVSAATASGIGAIFRAPLGGALLGAELLYRDDMESDAIMPSLVSSIVA
HHHCCCCCCCCEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FVVFGSVYGFEPIFGSLSGVQFSQPLQLIWFVLLGLTAGLMGKLYVEVFYSGTTLFDRLD
HHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KVPGWLVPAIGGLGVGLLGLLIPAALGTGYGSVQQEMFANNLVRMPLLMVLAIPFAKILS
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTLSIGSGGSGGVFGPGMVIGGATGAALWRLLEGLPGIPSSPMSFVIVGMIACFGAVAHA
HHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHC
PLGVLLMVGEMTGNLSMLAPGMIAVAVAGRVVGDTSIYTSQLKDRLEGRRVHAMGRVTKL
CHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHC
SEPKSDDEVASPKPAIKKPSGKNASLSKPAAEPLAKPQAKPATVPSPHPTTAPTPATHQR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
DTPRIVPEIIRAKDRKHELLARSPKPPNDPGPSSA
CCCHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TETAIHPEARAAWAKQLRRLRRALVPHRPKAFRDSLNRWLLMGTVIGIVSGVGAIIFFF
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CLKTGTHWLLETLGGYTPPGTLGEGGGVAGTRLTRPWAIPLIVAGGALVSGTLVYFVAPT
HHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHCEEEEEEECC
AKGHGTDNAIHAVHHDPTALKGKVAAVKIIASSLVIGSGGSGGREGPTAQISATVISSLC
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHH
KKLKIATPDARIAVSAATASGIGAIFRAPLGGALLGAELLYRDDMESDAIMPSLVSSIVA
HHHCCCCCCCCEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
FVVFGSVYGFEPIFGSLSGVQFSQPLQLIWFVLLGLTAGLMGKLYVEVFYSGTTLFDRLD
HHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KVPGWLVPAIGGLGVGLLGLLIPAALGTGYGSVQQEMFANNLVRMPLLMVLAIPFAKILS
HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTLSIGSGGSGGVFGPGMVIGGATGAALWRLLEGLPGIPSSPMSFVIVGMIACFGAVAHA
HHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHC
PLGVLLMVGEMTGNLSMLAPGMIAVAVAGRVVGDTSIYTSQLKDRLEGRRVHAMGRVTKL
CHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHC
SEPKSDDEVASPKPAIKKPSGKNASLSKPAAEPLAKPQAKPATVPSPHPTTAPTPATHQR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
DTPRIVPEIIRAKDRKHELLARSPKPPNDPGPSSA
CCCHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]